KEGG   ENZYME: 1.14.14.48
Entry
EC 1.14.14.48               Enzyme                                 
Name
jasmonoyl-L-amino acid 12-hydroxylase;
CYP94B1 (gene name);
CYP94B3 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
jasmonoyl-L-amino acid,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (12-hydroxylating)
Reaction(IUBMB)
a jasmonoyl-L-amino acid + [reduced NADPH---hemoprotein reductase] + O2 = a 12-hydroxyjasmonoyl-L-amino acid + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11806]
Reaction(KEGG)
R11806
Substrate
jasmonoyl-L-amino acid [CPD:C21509];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
12-hydroxyjasmonoyl-L-amino acid [CPD:C21696];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P450 (heme thiolate) enzyme found in plants. The enzyme acts on jasmonoyl-L-amino acid conjugates, catalysing the hydroxylation of the C-12 position of jasmonic acid. While the best studied substrate is (+)-7-epi-jasmonoyl-L-isoleucine, the enzyme was shown to be active with jasmonoyl-L-valine and jasmonoyl-L-phenylalanine, and is likely to be active with other jasmonoyl-amino acid conjugates.
History
EC 1.14.14.48 created 2017
Orthology
K20665  jasmonoyl-L-amino acid 12-hydroxylase
Genes
ATHAT3G01900(CYP94B2) AT3G48520(CYP94B3) AT5G63450(CYP94B1)
ALY9300923 9311979 9318229
CRB17874658 17885442 17892234
CSAT104711346 104720538 104726960 104741179 104762484 104763703 104773500 104780667 104791069
EUSEUTSA_v10005707mg EUTSA_v10011125mg
BRP103837470 103848967 103855044 103873137 103873527 117125840
BNA106351873 106364122 106382901 106405321 106409295 106428634 106430785 106431245 106443168 111208658 111213368
BOE106294052 106308148 106316769 106325388 106333823
RSZ108809281 108825449 108825450 108834123 108856870 108859677 108860504
THJ104818188 104821513 104822524
CPAP110807430 110820488
CIT102610480 102629732
CICCICLE_v10017920mg CICLE_v10019793mg
PVY116105682 116110514 116130733
MINC123195622 123211695 123223852
TCC108661791 18605092
GRA105781059 105788686 105798446 105802102
GHI107888050 107892164 107899717 107912087 107923036 107950185 107960454
GAB108454222 108459965 108466763 108477615
DZI111290941 111292713 111303810 111315893
EGR104416900 104416919 104419143 104422706 104434222 104434223 104434224 104434225
GMX100786130 100797785 100800427 100810294 100815295
GSJ114371922 114390206 114399625 114406286 114406288
PVUPHAVU_001G094200g PHAVU_010G074400g
VRA106753837 106773363
VAR108328352 108346692
VUN114165352 114171458 114180532
CCAJ109809705 109814574 109814578
APRC113858257
MTRMTR_3g080580 MTR_7g029275
CAM101507161 101511677
LJALj0g3v0055949.1(Lj0g3v0055949.1)
ADU107494813
AIP107605023
AHF112697200 112755098
LANG109344366
FVE101291070 105351203
RCN112178560 112188217
PPER18776547 18783641
PMUM103330340 103338054
PAVI110763155 110764345
PDUL117621128 117627092
MDM103404195 103409661 114826857
PXB103950019 103958049 103960885 103964059
ZJU107418019 107422044 107427926
MNT21388965 21394049
CSV101202989 101207598
CMO103491786 103502328
BHJ120071683 120084009
MCHA111007253
CMAX111466028 111481487 111485059
CMOS111429724 111435168 111440917 111441980
CPEP111780340 111790737 111792218 111793378
RCU8269245 8271459
JCU105634463 105640206
HBR110665259 110672803
MESC110605550 110616961 110631223
POP7484471 7495780
PEU105110545 105119630 105139432
PALZ118044706 118063345
JRE108994307 108994389 109013637 109021892
QSU112005217 112012674
QLO115964193 115976448
TWL120003769 120009844
VVI100245827 100251407
VRI117905025 117930395
SLY101262587 101263302 101263399 101263598
SPEN107008968 107013853 107013910 107013946
SOT102585085 102585163 102585498 102586169
CANN107860765 107862677 107862678 107866012
NTA107766030 107781029 107791570 107796932 107801204 107814708 107827898
NSY104214653 104217827 104232917 104241490
NTO104096380 104099650 104101764 104112197
NAU109223615 109239328 109239329 109244265
INI109171722
ITR116000316
SIND105166192 105166954
OEU111385797 111390746
EGT105951673 105964231
SSPL121747247 121751253 121761150 121763652
HAN110868163 110911674 110925776
ECAD122580456 122608462 122609539
LSV111877549 111920155
CCAV112511704 112513077
DCR108194102 108207998
CSIN114256410
BVG104893484
SOE110787294
CQI110724296 110724297 110739743
NNU104592904 104592906
MING122077558
TSS122653955
PSOM113302893
NCOL116246669 116247098 116249972 116250319 116251037
OSA4350521 4352145
DOSAOs12t0443000-00(Os12g0443000)
OBR102715166 121055701
BDI100823617
ATS109782849
TDC119288066 119349182
TAES123064325 123142111 123182780
SBI110435249
ZMA100381980 103652921(ts5)
SITA101768903 101774825
SVS117850389 117866210
PVIR120644341 120667857 120686613 120699353
PHAI112901932
PDA103706541
EGU105051167
MUS103979622 103989822 103996040
DCT110093521 110093522
PEQ110018426
ATR18438139
 » show all
Reference
1  [PMID:21576464]
  Authors
Koo AJ, Cooke TF, Howe GA
  Title
Cytochrome P450 CYP94B3 mediates catabolism and inactivation of the plant hormone jasmonoyl-L-isoleucine.
  Journal
Proc Natl Acad Sci U S A 108:9298-303 (2011)
DOI:10.1073/pnas.1103542108
  Sequence
[ath:AT3G48520]
Reference
2  [PMID:21849397]
  Authors
Kitaoka N, Matsubara T, Sato M, Takahashi K, Wakuta S, Kawaide H, Matsui H, Nabeta K, Matsuura H
  Title
Arabidopsis CYP94B3 encodes jasmonyl-L-isoleucine 12-hydroxylase, a key enzyme in the oxidative catabolism of jasmonate.
  Journal
Plant Cell Physiol 52:1757-65 (2011)
DOI:10.1093/pcp/pcr110
Reference
3  [PMID:22215670]
  Authors
Heitz T, Widemann E, Lugan R, Miesch L, Ullmann P, Desaubry L, Holder E, Grausem B, Kandel S, Miesch M, Werck-Reichhart D, Pinot F
  Title
Cytochromes P450 CYP94C1 and CYP94B3 catalyze two successive oxidation steps of plant hormone Jasmonoyl-isoleucine for catabolic turnover.
  Journal
J Biol Chem 287:6296-306 (2012)
DOI:10.1074/jbc.M111.316364
  Sequence
[ath:AT3G48520]
Reference
4  [PMID:24467969]
  Authors
Kitaoka N, Kawaide H, Amano N, Matsubara T, Nabeta K, Takahashi K, Matsuura H
  Title
CYP94B3 activity against jasmonic acid amino acid conjugates and the elucidation  of 12-O-beta-glucopyranosyl-jasmonoyl-L-isoleucine as an additional metabolite.
  Journal
Phytochemistry 99:6-13 (2014)
DOI:10.1016/j.phytochem.2013.12.019
Reference
5  [PMID:25210037]
  Authors
Koo AJ, Thireault C, Zemelis S, Poudel AN, Zhang T, Kitaoka N, Brandizzi F, Matsuura H, Howe GA
  Title
Endoplasmic reticulum-associated inactivation of the hormone jasmonoyl-L-isoleucine by multiple members of the cytochrome P450 94 family in Arabidopsis.
  Journal
J Biol Chem 289:29728-38 (2014)
DOI:10.1074/jbc.M114.603084
  Sequence
Reference
6  [PMID:26164240]
  Authors
Widemann E, Grausem B, Renault H, Pineau E, Heinrich C, Lugan R, Ullmann P, Miesch L, Aubert Y, Miesch M, Heitz T, Pinot F
  Title
Sequential oxidation of Jasmonoyl-Phenylalanine and Jasmonoyl-Isoleucine by multiple cytochrome P450 of the CYP94 family through newly identified aldehyde intermediates.
  Journal
Phytochemistry 117:388-99 (2015)
DOI:10.1016/j.phytochem.2015.06.027
  Sequence
[ath:AT3G48520]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.48
IUBMB Enzyme Nomenclature: 1.14.14.48
ExPASy - ENZYME nomenclature database: 1.14.14.48
BRENDA, the Enzyme Database: 1.14.14.48

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