KEGG   ENZYME: 1.14.14.54
Entry
EC 1.14.14.54               Enzyme                                 
Name
phenylacetate 2-hydroxylase;
CYP504;
phaA (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
phenylacetate,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (2-hydroxylating)
Reaction(IUBMB)
phenylacetate + [reduced NADPH---hemoprotein reductase] + O2 = (2-hydroxyphenyl)acetate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R05487]
Reaction(KEGG)
R05487
Substrate
phenylacetate [CPD:C07086];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
2-hydroxyphenylacetate [CPD:C05852];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
This cytochrome P-450 (heme-thiolate) enzyme, found in Aspergillus nidulans, is involved in the degradation of phenylacetate.
History
EC 1.14.14.54 created 2017
Pathway
ec00360  Phenylalanine metabolism
ec00643  Styrene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K10437  phenylacetate 2-hydroxylase
Genes
QSU112006183 112014358
PPAPAS_chr3_0864
DHADEHA2D03168g
PICPICST_49761
PGUPGUG_01534
SPAASPAPADRAFT_48604
CALCAALFM_C404360WA(CaO19.1411)
CTPCTRG_03930
COTCORT_0E04490
CDUCD36_44030
CTENCANTEDRAFT_117516
YLIYALI0F03663g
CLUCLUG_05046
CLUSA9F13_03g01892
CAURCJI97_001232
SLBAWJ20_2948
BNNFOA43_001054
BBRXBRETT_003642
OPAHPODL_00882 HPODL_02307
MGRMGG_04684
PPEIPpBr36_05425
FGRFGSG_03741
FPUFPSE_08438
FVRFVEG_08703
FOXFOXG_09779
NHENECHADRAFT_102133 NECHADRAFT_54339
TRETRIREDRAFT_4726
MAWMAC_08608
MAJMAA_10366
CMTCCM_02553
PLJVFPFJ_10353 VFPFJ_11202
CFJCFIO01_05930
SAPOSAPIO_CDS6228 SAPIO_CDS6571
ELAUCREL1_9178
PFYPFICI_13076 PFICI_15387
SSLSS1G_06874
BFUBCIN_11g01060
MBEMBM_06099
PSCOLY89DRAFT_640579 LY89DRAFT_698231
GLZGLAREA_00187 GLAREA_06077
ANIAN8078.2
AFMAFUA_5G01710
ACTACLA_003600
NFINFIA_040380
AORAO090003001361
ANGANI_1_128144(An16g01030) ANI_1_1740104(An12g04210)
AFVAFLA_000316
ALUCAKAW2_60307S AKAW2_60829S AKAW2_70076A
APUUAPUU_21170S APUU_41450A APUU_41480A
PCSPc21g14280(pahA) Pc22g02230
PDPPDIP_47610
TMFPMAA_085890 PMAA_087970 PMAA_098630
TRGTRUGW13939_03396 TRUGW13939_07699 TRUGW13939_10129
CIMCIMG_07017
CPWCPC735_033760
UREUREG_05848
PBLPAAG_08664
PBNPADG_07269
ABEARB_03198
TVETRV_03913
BGHBDBG_00176
PNOSNOG_06873 SNOG_07254
PTEPTT_06566 PTT_19143
BZECOCCADRAFT_96181 COCCADRAFT_97535
BSCCOCSADRAFT_148288 COCSADRAFT_148767
BORCOCMIDRAFT_40411 COCMIDRAFT_4778 COCMIDRAFT_7585
AALTCC77DRAFT_1030168 CC77DRAFT_779096
ZTRMYCGRDRAFT_32609(CYP-22) MYCGRDRAFT_83782(CYP-33)
PFJMYCFIDRAFT_185117 MYCFIDRAFT_201503
FFUCLAFUR5_00972 CLAFUR5_01627
BCOMBAUCODRAFT_188720
NPAUCRNP2_1360 UCRNP2_5654
TASAA1Q1_06336
SLASERLADRAFT_414563
UMAUMAG_01424
PFPPFL1_00039
 » show all
Reference
1  [PMID:10329644]
  Authors
Mingot JM, Penalva MA, Fernandez-Canon JM
  Title
Disruption of phacA, an Aspergillus nidulans gene encoding a novel cytochrome P450 monooxygenase catalyzing phenylacetate 2-hydroxylation, results in penicillin overproduction.
  Journal
J Biol Chem 274:14545-50 (1999)
DOI:10.1074/jbc.274.21.14545
  Sequence
[ani:AN8078.2]
Reference
2  [PMID:11544206]
  Authors
Rodriguez-Saiz M, Barredo JL, Moreno MA, Fernandez-Canon JM, Penalva MA, Diez B.
  Title
Reduced function of a phenylacetate-oxidizing cytochrome p450 caused strong genetic improvement in early phylogeny of penicillin-producing strains.
  Journal
J Bacteriol 183:5465-71 (2001)
DOI:10.1128/JB.183.19.5465-5471.2001
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.54
IUBMB Enzyme Nomenclature: 1.14.14.54
ExPASy - ENZYME nomenclature database: 1.14.14.54
BRENDA, the Enzyme Database: 1.14.14.54

DBGET integrated database retrieval system