KEGG   ENZYME: 1.14.14.70
Entry
EC 1.14.14.70               Enzyme                                 
Name
ent-sandaracopimaradiene 3-hydroxylase;
CYP701A;
OsKOL4
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
ent-sandaracopimaradiene,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (ent-sandaracopimaradien-3beta-ol-forming)
Reaction(IUBMB)
ent-sandaracopimaradiene + [reduced NADPH---hemoprotein reductase] + O2 = ent-sandaracopimaradien-3beta-ol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R11656]
Reaction(KEGG)
R11656
Substrate
ent-sandaracopimaradiene [CPD:C11877];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
ent-sandaracopimaradien-3beta-ol [CPD:C21560];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein isolated from Oryza sativa (rice). Participates in the pathway for the biosynthesis of oryzalexins, a group of related phytoalexins produced by rice. Can also use 9beta-pimara-7,15-diene as substrate (cf. EC 1.14.14.68, syn-pimaradiene 3-monooxygenase).
History
EC 1.14.14.70 created 2014 as EC 1.14.13.191, transferred 2018 to EC 1.14.14.70
Pathway
ec00904  Diterpenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K21719  ent-sandaracopimaradiene 3-hydroxylase / syn-pimaradiene 3-monooxygenase
Genes
OSA4341340 4341343
DOSAOs06t0568600-01(Os06g0568600) Os06t0569500-01(Os06g0569500)
OBR102701085
Reference
1  [PMID:22247270]
  Authors
Wang Q, Hillwig ML, Wu Y, Peters RJ
  Title
CYP701A8: a rice ent-kaurene oxidase paralog diverted to more specialized diterpenoid metabolism.
  Journal
Plant Physiol 158:1418-25 (2012)
DOI:10.1104/pp.111.187518
  Sequence
[osa:4341343]
Reference
2  [PMID:23795884]
  Authors
Wu Y, Wang Q, Hillwig ML, Peters RJ
  Title
Picking sides: distinct roles for CYP76M6 and CYP76M8 in rice oryzalexin biosynthesis.
  Journal
Biochem J 454:209-16 (2013)
DOI:10.1042/BJ20130574
  Sequence
[osa:4341343]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.70
IUBMB Enzyme Nomenclature: 1.14.14.70
ExPASy - ENZYME nomenclature database: 1.14.14.70
BRENDA, the Enzyme Database: 1.14.14.70

DBGET integrated database retrieval system