KEGG   ENZYME: 1.14.14.86
Entry
EC 1.14.14.86               Enzyme                                 
Name
ent-kaurene monooxygenase;
ent-kaurene oxidase (misleading)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
ent-kaur-16-ene,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (hydroxylating)
Reaction(IUBMB)
ent-kaur-16-ene + 3 [reduced NADPH---hemoprotein reductase] + 3 O2 = ent-kaur-16-en-19-oate + 3 [oxidized NADPH---hemoprotein reductase] + 4 H2O (overall reaction) [RN:R10066];
(1a) ent-kaur-16-ene + [reduced NADPH---hemoprotein reductase] + O2 = ent-kaur-16-en-19-ol + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R06291];
(1b) ent-kaur-16-en-19-ol + [reduced NADPH---hemoprotein reductase] + O2 = ent-kaur-16-en-19-al + [oxidized NADPH---hemoprotein reductase] + 2 H2O [RN:R06292];
(1c) ent-kaur-16-en-19-al + [reduced NADPH---hemoprotein reductase] + O2 = ent-kaur-16-en-19-oate + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R06293]
Reaction(KEGG)
Substrate
ent-kaur-16-ene [CPD:C06090];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007];
ent-kaur-16-en-19-ol [CPD:C11872];
ent-kaur-16-en-19-al [CPD:C11873]
Product
ent-kaur-16-en-19-oate [CPD:C11874];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001];
ent-kaur-16-en-19-ol [CPD:C11872];
ent-kaur-16-en-19-al [CPD:C11873]
Comment
A cytochrome P-450 (heme thiolate) protein found in plants. Catalyses three successive oxidations of the 4-methyl group of ent-kaurene giving kaurenoic acid.
History
EC 1.14.14.86 created 2002 as EC 1.14.13.78, transferred 2018 to EC 1.14.14.86
Pathway
ec00904  Diterpenoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K04122  ent-kaurene oxidase
K21292  ent-kaurene oxidase
Genes
CRG105335893 117682816
ATHAT5G25900(GA3)
ALY9308254
CRB17884655
CSAT104706907 104736615 104771068
EUSEUTSA_v10004043mg
BRP103874435
BNA106348557 106348758
BOE106301555
RSZ108833173 108853944
THJ104803409 104806957
CPAP110825592 110825600 110825618
CIT102626273 102627235
CICCICLE_v10000884mg CICLE_v10000902mg CICLE_v10001179mg
PVY116137812
MINC123229342
TCC18591283
GRA105774074
GHI107935613 121218163
GAB108471487 108480403
DZI111312649
EGR104449362 104449363
GMX100802970(CYP701A16) 100814223
GSJ114377322 114388284
PVUPHAVU_005G183600g
VRA106762922
VAR108336168
VUN114194654
CCAJ109812642 109817002
APRC113857239
MTRMTR_2g105360
CAM101492348 101492682
LJALj0g3v0151459.1(Lj0g3v0151459.1) Lj0g3v0218449.1(Lj0g3v0218449.1) Lj3g3v0002210.2(Lj3g3v0002210.2)
ADU107459820 107483258
AIP107618674 107638960
AHF112703820 112741508 112771108 112796212
LANG109337304 109337306 109350784
FVE101315406
RCN112172744
PPER18791732
PMUM103320777 103320921
PAVI110762553
PDUL117616384
MDM103441571 103441598
PXB103954018 103955469 108865311
ZJU107420337
MNT21397575
CSV101209283
CMO103493505
BHJ120086045
MCHA111010641
CMAX111476578 111481118
CMOS111442772 111446712
CPEP111788623 111797494
RCU8260887
JCU105633700(EKO)
HBR110636133 110636190
MESC110614910
POP18096306
PEU105120662
PALZ118028112
JRE108992364
QSU112002318 112032780 112032781
QLO115974246 115977868
TWL120009866
VVI100255742
VRI117907815
SLY101267112
SPEN107016073
SOT102588924 102589573
CANN107867102
NTA107766539 107772573
NSY104230322
NTO104089658
NAU109224931
INI109149104(EKO1)
ITR116023321
SIND105159190 105159193 105166213
OEU111381791 111394477
EGT105965462
SSPL121766036 121802241 121809438
HAN110879488 110879531 110879533 110899627 110899628 118479237
ECAD122579177 122589190
LSV111901864 111912922 111912924
CCAV112503346 112503348 112503349 112514541
DCR108192836 108221827 108222415 108223735 108226232 108226927
CSIN114260575 114314457
BVG104883634
SOE110783160
CQI110692482 110727365
NNU104595848
MING122068825 122076995
TSS122662774
PSOM113286201 113294049 113294437 113330131 113337380 113345845
NCOL116255470
OSA4341347 9269379
DOSAOs06t0569900-01(Os06g0569900) Os06t0570100-01(Os06g0570100)
OBR102719778
BDI100826923 100828145 100834998
ATS109764879 109786379
TDC119331213 119332455 119340853 119351884 119365396 119368209 119368221
TAES123041900 123041901 123046473 123049868 123148035 123152475 123161689 123168202 123185940
SBI8065021
ZMA100274015 103638615
SITA101769883 101770285 101770692
SVS117851887 117853039 117853040
PVIR120670617 120670803
PHAI112889472 112889821
PDA103702042 103709580
EGU105040426 105047970
MUS103986614
DCT110093775 110099182
PEQ110032443 110032601
AOF109822294
ATR18425989 18443400
SMOSELMODRAFT_119177 SELMODRAFT_74427(CYP701C1)
PPP112273131 112273132
PPEIPpBr36_02038 PpBr36_02215 PpBr36_07524 PpBr36_09510
FVRFVEG_16575
TRETRIREDRAFT_56687
TRRM419DRAFT_127652
CHIGCH63R_13014 CH63R_14245
BFUBCIN_01g04880
ANIAN1598.2
NFINFIA_009830
ANGANI_1_1208164(An18g02700) ANI_1_2452094(An11g09260)
ALUCAKAW2_21192A
APUUAPUU_31775A
TRGTRUGW13939_10606
CIMCIMG_03089
CPWCPC735_010550
UREUREG_02869
ABEARB_00548
TVETRV_00376
PTEPTT_08290
BZECOCCADRAFT_112173
BSCCOCSADRAFT_185928
BORCOCMIDRAFT_109515 COCMIDRAFT_6219
ZTRMYCGRDRAFT_34461(CYP-58)
FFUCLAFUR5_07314
NPAUCRNP2_330
MOREE1B28_001684 E1B28_003709 E1B28_005347 E1B28_005657 E1B28_006548 E1B28_007143 E1B28_008402 E1B28_009754 E1B28_011394
 » show all
Reference
1  [PMID:2223832]
  Authors
Ashman PJ, Mackenzie A, Bramley PM.
  Title
Characterization of ent-kaurene oxidase activity from Gibberella fujikuroi.
  Journal
Biochim Biophys Acta 1036:151-7 (1990)
DOI:10.1016/0304-4165(90)90027-T
Reference
2  [PMID:1397591]
  Authors
Archer C, Ashman PJ, Hedden P, Bowyer JR, Bramley PM.
  Title
Purification of ent-kaurene oxidase from Gibberella fujikuroi and Cucurbita maxima.
  Journal
Biochem Soc Trans 20:218S (1992)
DOI:10.1042/bst020218s
Reference
3  [PMID:9952446]
  Authors
Helliwell CA, Poole A, Peacock WJ, Dennis ES.
  Title
Arabidopsis ent-kaurene oxidase catalyzes three steps of gibberellin biosynthesis.
  Journal
Plant Physiol 119:507-10 (1999)
DOI:10.1104/pp.119.2.507
  Sequence
[ath:AT5G25900]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.86
IUBMB Enzyme Nomenclature: 1.14.14.86
ExPASy - ENZYME nomenclature database: 1.14.14.86
BRENDA, the Enzyme Database: 1.14.14.86
CAS: 149565-67-3

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