KEGG   ENZYME: 1.14.14.88
Entry
EC 1.14.14.88               Enzyme                                 
Name
isoflavone 3'-hydroxylase;
isoflavone 3'-monooxygenase;
CYP81E9
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
formononetin,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (3'-hydroxylating)
Reaction(IUBMB)
formononetin + [reduced NADPH---hemoprotein reductase] + O2 = calycosin + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R03006]
Reaction(KEGG)
R03006;
(other) R07776
Substrate
formononetin [CPD:C00858];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
calycosin [CPD:C01562];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein. Also acts on biochanin A and other isoflavones with a 4'-methoxy group. Involved in the biosynthesis of the pterocarpin phytoalexins medicarpin and maackiain.
History
EC 1.14.14.88 created 1992 as EC 1.14.13.52, transferred 2018 to EC 1.14.14.88
Pathway
ec00943  Isoflavonoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K22932  isoflavone 3'-hydroxylase
Genes
GMX100784120
GSJ114424365
PVUPHAVU_002G187800g
VRA106766214
VAR108319111
VUN114176660
CCAJ109806741
APRC113845824 113858428
MTRMTR_2g437880
CAM101503563(cyp81E5) 101503888(cyp81E4)
ADU107482085 107482086 107483700
AIP107631471 107634717 107639094
AHF112734653 112734656 112734657 112741794 112792436 112796479
 » show all
Reference
1
  Authors
Hinderer, W., Flentje, U. and Barz, W.
  Title
Microsomal isoflavone 2'-hydroxylases and 3'-hydroxylases from chickpea (Cicer arietinum L) cell-suspensions induced for pterocarpan phytoalexin formation.
  Journal
FEBS Lett 214:101-106 (1987)
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.88
IUBMB Enzyme Nomenclature: 1.14.14.88
ExPASy - ENZYME nomenclature database: 1.14.14.88
BRENDA, the Enzyme Database: 1.14.14.88
CAS: 110183-50-1

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