KEGG   ENZYME: 1.14.14.93
Entry
EC 1.14.14.93               Enzyme                                 

Name
3,9-dihydroxypterocarpan 6a-monooxygenase;
3,9-dihydroxypterocarpan 6a-hydroxylase;
3,9-dihydroxypterocarpan 6alpha-monooxygenase (erroneous);
CYP93A1 (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
(6aR,11aR)-3,9-dihydroxypterocarpan,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (6a-hydroxylating)
Reaction(IUBMB)
(6aR,11aR)-3,9-dihydroxypterocarpan + [reduced NADPH---hemoprotein reductase] + O2 = (6aS,11aS)-3,6a,9-trihydroxypterocarpan + [oxidized NADPH---hemoprotein reductase] + H2O [RN:R03452]
Reaction(KEGG)
R03452
Substrate
(6aR,11aR)-3,9-dihydroxypterocarpan [CPD:C04271];
[reduced NADPH---hemoprotein reductase] [CPD:C03024];
O2 [CPD:C00007]
Product
(6aS,11aS)-3,6a,9-trihydroxypterocarpan [CPD:C01263];
[oxidized NADPH---hemoprotein reductase] [CPD:C03161];
H2O [CPD:C00001]
Comment
A cytochrome P-450 (heme-thiolate) protein found in soybean. The product of the reaction is the biosynthetic precursor of the glyceollin phytoalexins.
History
EC 1.14.14.93 created 1989 as EC 1.14.13.28, transferred 2018 to EC 1.14.14.93
Pathway
ec00943  Isoflavonoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K13261  3,9-dihydroxypterocarpan 6a-monooxygenase
Genes
GMX: 100776878(CYP93A1) 100808223
GSJ: 114399397 114406771
Reference
1  [PMID:6540173]
  Authors
Hagmann ML, Heller W, Grisebach H.
  Title
Induction of phytoalexin synthesis in soybean. Stereospecific 3,9-dihydroxypterocarpan 6a-hydroxylase from elicitor-induced soybean cell cultures.
  Journal
Eur J Biochem 142:127-31 (1984)
DOI:10.1111/j.1432-1033.1984.tb08259.x
Reference
2  [PMID:9720921]
  Authors
Schopfer CR, Kochs G, Lottspeich F, Ebel J
  Title
Molecular characterization and functional expression of dihydroxypterocarpan 6a-hydroxylase, an enzyme specific for pterocarpanoid phytoalexin biosynthesis in soybean (Glycine max L.).
  Journal
FEBS Lett 432:182-6 (1998)
DOI:10.1016/S0014-5793(98)00866-7
  Sequence
[gmx:100776878]
Other DBs
ExplorEnz - The Enzyme Database: 1.14.14.93
IUBMB Enzyme Nomenclature: 1.14.14.93
ExPASy - ENZYME nomenclature database: 1.14.14.93
BRENDA, the Enzyme Database: 1.14.14.93
CAS: 92584-16-2

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