CYP82N4 (gene name)
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen into the other donor
(S)-N-methylcanadine,[reduced NADPH---hemoprotein reductase]:oxygen oxidoreductase (14-hydroxylating)
(S)-N-methylcanadine + [reduced NADPH---hemoprotein reductase] + O2 = allocryptopine + [oxidized NADPH---hemoprotein reductase] + H2O [RN:
[reduced NADPH---hemoprotein reductase] [CPD:
[oxidized NADPH---hemoprotein reductase] [CPD:
A cytochrome P-450 (heme-thiolate) protein found in plants.
EC 184.108.40.206 created 1990 as EC 220.127.116.11, transferred 2018 to EC 18.104.22.168
ec00950 Isoquinoline alkaloid biosynthesis
ec01110 Biosynthesis of secondary metabolites
K21692 methyltetrahydroprotoberberine 14-monooxygenase
Rueffer, M. and Zenk, M.H.
Enzymatic formation of protopines by a microsomal cytochrome-P-450 system of Corydalis vaginans.
Tetrahedron Lett 28:5307-5310 (1987)
Beaudoin GA, Facchini PJ
Isolation and characterization of a cDNA encoding (S)-cis-N-methylstylopine 14-hydroxylase from opium poppy, a key enzyme in sanguinarine biosynthesis.