camphor 5-exo-methylene hydroxylase;
camphor methylene hydroxylase
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
(+)-camphor,reduced putidaredoxin:oxygen oxidoreductase (5-hydroxylating)
(+)-camphor + reduced putidaredoxin + O2 = (+)-exo-5-hydroxycamphor + oxidized putidaredoxin + H2O [RN:
oxidized putidaredoxin [CPD:
A heme-thiolate protein (P-450). Also acts on (-)-camphor and 1,2-campholide, forming 5-exo-hydroxy-1,2-campholide.
EC 184.108.40.206 created 1972, modified 1986
K21569 camphor 5-monooxygenase
Hedegaard J, Gunsalus IC.
Mixed function oxidation. IV. An induced methylene hydroxylase in camphor oxidation.
J Biol Chem 240:4038-43 (1965)
Tyson CA, Lipscomb JD, Gunsalus IC.
The role of putidaredoxin and P450 cam in methylene hydroxylation.
J Biol Chem 247:5777-84 (1972)
ExPASy - ENZYME nomenclature database: 220.127.116.11
UM-BBD (Biocatalysis/Biodegradation Database): 18.104.22.168