tylH1 (gene name)
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen into the other donor
20-oxo-5-O-beta-mycaminosyltylactone,reduced ferredoxin:oxygen oxidoreductase (23-hydroxylating)
20-oxo-5-O-beta-mycaminosyltylactone + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H+ + O2 = 5-O-beta-mycaminosyltylonolide + 2 oxidized ferredoxin [iron-sulfur] cluster + H2O [RN:
reduced ferredoxin [iron-sulfur] cluster [CPD:
oxidized ferredoxin [iron-sulfur] cluster [CPD:
A cytochrome P-450 (heme-thiolate) protein. Involved in the biosynthetic pathway of the macrolide antibiotic tylosin, which is produced by several species of Streptomyces bacteria.
EC 188.8.131.52 created 2014 as EC 184.108.40.206, transferred 2018 to EC 220.127.116.11
ec00522 Biosynthesis of 12-, 14- and 16-membered macrolides
ec01110 Biosynthesis of secondary metabolites
K15991 20-oxo-5-O-mycaminosyltylactone 23-monooxygenase
Baltz RH, Seno ET
Properties of Streptomyces fradiae mutants blocked in biosynthesis of the macrolide antibiotic tylosin.
Reeves CD, Ward SL, Revill WP, Suzuki H, Marcus M, Petrakovsky OV, Marquez S, Fu H, Dong SD, Katz L
Production of hybrid 16-membered macrolides by expressing combinations of polyketide synthase genes in engineered Streptomyces fradiae hosts.