KEGG   ENZYME: 1.14.17.1
Entry
EC 1.14.17.1                Enzyme                                 

Name
dopamine beta-monooxygenase;
dopamine beta-hydroxylase;
MDBH (membrane-associated dopamine beta-monooxygenase);
SDBH (soluble dopamine beta-monooxygenase);
dopamine-B-hydroxylase;
3,4-dihydroxyphenethylamine beta-oxidase;
4-(2-aminoethyl)pyrocatechol beta-oxidase;
dopa beta-hydroxylase;
dopamine beta-oxidase;
dopamine hydroxylase;
phenylamine beta-hydroxylase;
(3,4-dihydroxyphenethylamine)beta-mono-oxygenase;
DbetaM (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With reduced ascorbate as one donor, and incorporation of one atom of oxygen into the other donor
Sysname
dopamine,ascorbate:oxygen oxidoreductase (beta-hydroxylating)
Reaction(IUBMB)
dopamine + 2 ascorbate + O2 = noradrenaline + 2 monodehydroascorbate + H2O [RN:R02535]
Reaction(KEGG)
R02535
Substrate
dopamine [CPD:C03758];
ascorbate [CPD:C00072];
O2 [CPD:C00007]
Product
noradrenaline [CPD:C00547];
monodehydroascorbate [CPD:C01041];
H2O [CPD:C00001]
Comment
A copper protein. The enzyme, found in animals, binds two copper ions with distinct roles during catalysis. Stimulated by fumarate.
History
EC 1.14.17.1 created 1965 as EC 1.14.2.1, transferred 1972 to EC 1.14.17.1, modified 2020
Pathway
ec00350  Tyrosine metabolism
ec01100  Metabolic pathways
Orthology
K00503  dopamine beta-monooxygenase
Genes
HSA: 1621(DBH)
PTR: 464827(DBH)
PPS: 100985587(DBH)
GGO: 101154486(DBH)
PON: 100461514(DBH)
NLE: 100597609(DBH)
MCC: 574105(DBH)
MCF: 102139527(DBH)
CSAB: 103239676(DBH)
CATY: 105580523(DBH)
PANU: 101016746
RRO: 104669786(DBH)
RBB: 108513277(DBH)
TFN: 117099202(DBH)
PTEH: 111523984(DBH)
CJC: 100406041(DBH)
SBQ: 101046079(DBH)
MMUR: 105880739(DBH)
MMU: 13166(Dbh)
MCAL: 110290214(Dbh)
MPAH: 110319201(Dbh)
RNO: 25699(Dbh)
MCOC: 116089882(Dbh)
MUN: 110557516(Dbh)
CGE: 100761411(Dbh)
PLEU: 114684222(Dbh)
NGI: 103742818(Dbh)
HGL: 101724312(Dbh)
CCAN: 109677868(Dbh)
OCU: 103347199(DBH)
TUP: 102468703(DBH)
CFA: 448806(DBH)
VVP: 112906947(DBH)
VLG: 121473853(DBH)
AML: 100480347(DBH)
UMR: 103679922(DBH)
UAH: 113266575(DBH)
ORO: 101362871(DBH)
ELK: 111146214
MPUF: 101687995(DBH)
EJU: 114201020(DBH)
MLX: 118018100(DBH)
FCA: 101082957(DBH)
PTG: 102961078(DBH)
PPAD: 109258287(DBH)
AJU: 106988258(DBH)
HHV: 120247318(DBH)
BTA: 280758(DBH)
BOM: 102286194(DBH)
BIU: 109566610(DBH)
BBUB: 102409301(DBH)
CHX: 102176244(DBH)
OAS: 114113851(DBH)
ODA: 120852173(DBH)
SSC: 733609(DBH)
CFR: 102523816(DBH)
CBAI: 105068611(DBH)
CDK: 105106327(DBH)
BACU: 103001548(DBH)
LVE: 103082302(DBH)
OOR: 101269198(DBH)
DLE: 111177039(DBH)
PCAD: 102990638(DBH)
ECB: 791247(DBH)
EPZ: 103564043(DBH)
EAI: 106845350(DBH)
MYB: 102264141(DBH)
MYD: 102763656(DBH)
MMYO: 118666900(DBH)
MNA: 107524669(DBH)
HAI: 109381156(DBH)
DRO: 112306436(DBH)
SHON: 118980160(DBH)
AJM: 119036119(DBH)
MMF: 118629977(DBH)
PALE: 102882740(DBH)
PGIG: 120618872(DBH)
RAY: 107506265(DBH)
MJV: 108398386(DBH)
TOD: 119231230(DBH)
LAV: 100675803(DBH)
TMU: 101344432
MDO: 100018814(DBH)
SHR: 100925655(DBH)
PCW: 110205374(DBH)
OAA: 100073905(DBH)
GGA: 395549(DBH)
PCOC: 116234079(DBH)
MGP: 100544779(DBH)
CJO: 107321868(DBH)
NMEL: 110406867(DBH)
APLA: 101793381(DBH)
ACYG: 106044068(DBH)
TGU: 100224356(DBH)
LSR: 110477727(DBH)
SCAN: 103818963(DBH)
PMOA: 120512423(DBH)
GFR: 102038113(DBH)
FAB: 101810825(DBH)
PHI: 102100810(DBH)
PMAJ: 107211985(DBH)
CCAE: 111937045(DBH)
CCW: 104688340(DBH)
ETL: 114059130(DBH)
FPG: 101923335(DBH)
FCH: 102048380(DBH)
CLV: 102097674(DBH)
EGZ: 104129357(DBH)
NNI: 104023198(DBH)
ACUN: 113486646(DBH)
PADL: 103915345(DBH)
AAM: 106484479(DBH)
ASN: 102377750(DBH)
AMJ: 102562465(DBH)
CPOO: 109313992(DBH)
GGN: 109293013(DBH)
PSS: 102455557(DBH)
CMY: 102935981(DBH)
CPIC: 101944381(DBH)
TST: 117867238(DBH)
CABI: 116817450(DBH)
ACS: 100566843(dbh)
PVT: 110089291(DBH)
PBI: 103050577(DBH)
PMUR: 107291028(DBH)
TSR: 106552853(DBH)
PGUT: 117671327(DBH)
PMUA: 114588914(DBH)
ZVI: 118084255(DBH)
GJA: 107106563(DBH)
XLA: 108698178(dbh.L) 108699900(dbh.S)
XTR: 100492247(dbh)
NPR: 108792841(DBH)
DRE: 30505(dbh)
SGH: 107595570(dbh) 107597665
IPU: 108276829(dbh)
PHYP: 113544042(dbh)
AMEX: 103038160(dbh)
EEE: 113576331(dbh)
TRU: 101066621(dbh)
LCO: 104923914(dbh)
NCC: 104968179(dbh)
CGOB: 115016923(dbh)
ELY: 117269510(dbh)
PLEP: 121960296(dbh)
SLUC: 116034955
ECRA: 117959052(dbh)
PFLV: 114571332
GAT: 120832241(dbh)
MSAM: 119882201 119914708(dbh)
CUD: 121525273(dbh)
MZE: 101464817(dbh)
ONL: 100708156(dbh)
OAU: 116335170(dbh)
OLA: 101157870(dbh)
OML: 112162907(dbh)
XMA: 102218690(dbh)
XCO: 114149436(dbh)
XHE: 116724091(dbh)
PRET: 103473626(dbh)
CVG: 107088692(dbh)
CTUL: 119782614(dbh)
NFU: 107372400(dbh)
KMR: 108232649(dbh)
ALIM: 106514559(dbh)
AOCE: 111567020(dbh)
CSEM: 103389065(dbh)
POV: 109626136(dbh)
LCF: 108879254(dbh)
SDU: 111238762(dbh)
SLAL: 111666075(dbh)
XGL: 120806361(dbh)
HCQ: 109517071(dbh)
BPEC: 110174164(dbh)
MALB: 109963454(dbh)
SASA: 106568424(dbh)
OTW: 112257386
OMY: 110536983(dbh)
SALP: 111980057
SNH: 120025633(dbh)
ELS: 105014776(dbh)
SFM: 108934136(dbh)
PKI: 111854168(dbh)
AANG: 118212852(dbh)
LOC: 102682868(dbh)
ARUT: 117422501(dbh)
LCM: 102359119(DBH)
CMK: 103182970(dbh)
BFO: 118421819
BBEL: 109482305
CIN: 100176680
APLC: 110976390
SKO: 102804261
DME: Dmel_CG1543(Tbh)
DER: 6551562
DSE: 6620197
DSI: Dsimw501_GD16874(Dsim_Tbh)
DAN: 6503980
DSR: 110178084
DPE: 6600415
DMN: 108165110
DWI: 6641182
DGR: 6566217
DAZ: 108620140
DNV: 115564952
DHE: 111598821
DVI: 6633815
CCAT: 101458576
MDE: 101897009
LCQ: 111684501
ACOZ: 120959168
AARA: 120903805
AAG: 5574019
CPII: 120420172
AME: 751102(Tbh)
ACER: 107998169
BIM: 100743458
BBIF: 117209243
BTER: 100649446
CCAL: 108629262
OBB: 114880623
MGEN: 117217919
NMEA: 116432070
CGIG: 122398220
SOC: 105201088
MPHA: 105837371
AEC: 105145835
ACEP: 105618961
PBAR: 105434118
VEM: 105570585
HST: 105182135
DQU: 106743489
CFO: 105258572
FEX: 115236740
LHU: 105668349
PGC: 109857916
OBO: 105277857
PCF: 106792493
NVI: 100119029
CSOL: 105359023
MDL: 103579714
CCIN: 107268727
TCA: 663011(TcTBH)
ATD: 109600593
AGB: 108905603
LDC: 111509121
NVL: 108563606
APLN: 108737152
PPYR: 116179966
OTU: 111417408
BMOR: 100862766(Tbh)
BMAN: 114239450
MSEX: 115439887
BANY: 112048211
PMAC: 106717834
PPOT: 106108479
PXU: 106116017
PRAP: 110994553
ZCE: 119838682
HAW: 110383622
TNL: 113501985
API: 100166363
DNX: 107169334
AGS: 114121829
RMD: 113558464
BTAB: 109037049
CLEC: 106667484
NLU: 111053896
ZNE: 110838985
CSEC: 111873648
FCD: 110851639
DMK: 116927591
HAME: 121860904
HAZT: 108669948
EAF: 111710478
DSV: 119437209
RSAN: 119400178
RMP: 119161520
VDE: 111253578
VJA: 111272129
TUT: 107360804
DPTE: 113793264
CSCU: 111615103
PTEP: 107447331
SDM: 118182174
CEL: CELE_H13N06.6(tbh-1)
CBR: CBG07714(Cbr-tbh-1)
OBI: 106875923
 » show all
Reference
1  [PMID:14416204]
  Authors
LEVIN EY, LEVENBERG B, KAUFMAN S.
  Title
The enzymatic conversion of 3,4-dihydroxyphenylethylamine to norepinephrine.
  Journal
J Biol Chem 235:2080-6 (1960)
Reference
2  [PMID:5846992]
  Authors
Friedman S, Kaufman S.
  Title
3,4-dihydroxyphenylethylamine beta-hydroxylase. Physical properties, copper content, and role of copper in the catalytic acttivity.
  Journal
J Biol Chem 240:4763-73 (1965)
Reference
3  [PMID:7388045]
  Authors
Skotland T, Ljones T
  Title
Direct spectrophotometric detection of ascorbate free radical formed by dopamine  beta-monooxygenase and by ascorbate oxidase.
  Journal
Biochim Biophys Acta 630:30-5 (1980)
DOI:10.1016/0304-4165(80)90134-8
Reference
4  [PMID:12966104]
  Authors
Evans JP, Ahn K, Klinman JP
  Title
Evidence that dioxygen and substrate activation are tightly coupled in dopamine beta-monooxygenase. Implications for the reactive oxygen species.
  Journal
J Biol Chem 278:49691-8 (2003)
DOI:10.1074/jbc.M300797200
Other DBs
ExplorEnz - The Enzyme Database: 1.14.17.1
IUBMB Enzyme Nomenclature: 1.14.17.1
ExPASy - ENZYME nomenclature database: 1.14.17.1
BRENDA, the Enzyme Database: 1.14.17.1
CAS: 9013-38-1

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