KEGG   ENZYME: 1.14.19.41
Entry
EC 1.14.19.41               Enzyme                                 
Name
sterol 22-desaturase;
ERG5 (gene name);
CYP710A (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With oxidation of a pair of donors resulting in the reduction of O2 to two molecules of water
Sysname
ergosta-5,7,24(28)-trien-3beta-ol,NADPH:oxygen oxidoreductase (22,23-dehydrogenating)
Reaction(IUBMB)
ergosta-5,7,24(28)-trien-3beta-ol + NADPH + H+ + O2 = ergosta-5,7,22,24(28)-tetraen-3beta-ol + NADP+ + 2 H2O [RN:R07506]
Reaction(KEGG)
R07506;
(other) R07489 R11097
Substrate
ergosta-5,7,24(28)-trien-3beta-ol [CPD:C15780];
NADPH [CPD:C00005];
H+ [CPD:C00080];
O2 [CPD:C00007]
Product
ergosta-5,7,22,24(28)-tetraen-3beta-ol [CPD:C05440];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
A heme-thiolate protein (P-450). The enzyme, found in yeast and plants, catalyses the introduction of a double bond between the C-22 and C-23 carbons of certain sterols. In yeast the enzyme acts on ergosta-5,7,24(28)-trien-3beta-ol, a step in the biosynthesis of ergosterol. The enzyme from the plant Arabidopsis thaliana acts on sitosterol and 24-epi-campesterol, producing stigmasterol and brassicasterol, respectively.
History
EC 1.14.19.41 created 2015
Pathway
ec00100  Steroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K09831  sterol 22-desaturase
K09832  sterol 22-desaturase
Genes
ATHAT2G28850(CYP710A3) AT2G28860(CYP710A4) AT2G34490(CYP710A2) AT2G34500(CYP710A1)
ALY9317081 9317405 9317406
CRB17887986 17889424 17889482
CSAT104699232 104699233 104700630 104750004 104750006 104753438 104781842 104781843 104786110 104786111 104786814 104789238 104789239 104789240 104792202 104792203
EUSEUTSA_v10016547mg EUTSA_v10016552mg
BRP103865343 103867449
BNA106396203 106432759 106432788 106447495
BOE106339100 106339917 106340751 106341405
RSZ108807244 108833237 108852806
THJ104814293
CPAP110807637
CIT102618952
CICCICLE_v10014973mg
PVY116141721
MINC123192456 123218316
TCC18596516
GRA105769297 105769298 105772027 105774040
GHI107901888 107905877 107905878 107942637 121203644
GAB108452825 108452842
DZI111280976 111318668
EGR104440796
GMX100787616 100808148
GSJ114377625 114387884
PVUPHAVU_006G163800g
VRA106776160
VAR108340983
VUN114188144
CCAJ109789198
APRC113852140
MTRMTR_2g019640
CAM101489453
ADU107468177
AIP107623676
AHF112714308 112782610
LANG109328906 109329075 109345270
FVE105350241
RCN112166774
PPER18778941
PMUM103325114
PAVI110749808 110773983
PDUL117625864
MDM103434989
ZJU107413371
MNT21404536
CSV101221194
CMO103484375
BHJ120074625
CMAX111478743 111496674
CMOS111433344 111433531
CPEP111782177 111803380
HBR110640583 110645097
MESC110609904 110621253
POP7472572
PEU105117943
PALZ118050840
JRE109021374
QSU112004265 112009839 112037010
QLO115980600 115992293
TWL119995149
VVI100255505
VRI117922864
SLY100136886(SD1)
SPEN107011895
SOT102581211
CANN107859112
NTA107763057 107771426(SD1)
NSY104234654
NTO104096813
NAU109208410
INI109192033
ITR116017561
SIND105167297 105172499
OEU111407278 111412227
EGT105951371
SSPL121805298 121808148
HAN110911670 110937398
ECAD122593276
LSV111890946
CCAV112504656
DCR108199974
CSIN114313482
BVG104883528
SOE110778797 110778903
CQI110730201 110732522
NNU104597733
MING122060078 122092751
TSS122639679 122650136
PSOM113329902 113338741 113345304 113355287
NCOL116265070
OSA4325562 4325563 4325564 9267882
DOSAOs01t0210900-01(Os01g0210900) Os01t0211000-00(Os01g0211000) Os01t0211200-00(Os01g0211200) Os01t0211600-01(Os01g0211600)
OBR102709230 102711741
BDI100827562
ATS109746138 109757150 109772439
TDC119267541 119275444 119292328 119298617 119304413 119338528 119338529 119343557 119344120
TAES123060655 123069249 123077753 123094069 123099277 123157569 123162932
TUA125507657 125543216
SBI8079522
ZMA107305676
SITA101764868 101770311
SVS117837101 117858509
PVIR120676345 120705614
PHAI112893543
PDA103702545 103703021
EGU105040643 105048162
MUS103995072 103999677 104000847
DCT110110071
PEQ110029296
AOF109828814
ATR18435199
SMOSELMODRAFT_234389 SELMODRAFT_75218(CYP710A21)
PPP112277322 112285903
SCEYMR015C(ERG5)
SEUBDI49_4102
SPAOSPAR_M01390
AGOAGOS_ABR053C
KLAKLLA0_D11242g
KMXKLMA_60417(cyp524A1)
LTHKLTH0D07832g
VPOKpol_1056p10
ZROZYRO0E06204g
CGRCAGL0M07656g
NCSNCAS_0A12700(NCAS0A12700)
NDINDAI_0B01560(NDAI0B01560)
TPFTPHA_0I02420(TPHA0I02420)
TBLTBLA_0H02650(TBLA0H02650)
TDLTDEL_0B06510(TDEL0B06510)
TGBHG536_0G01250
KAFKAFR_0A02110(KAFR0A02110)
KNGKNAG_0M02100(KNAG0M02100)
ZMKHG535_0G03560
PPAPAS_chr3_0507
DHADEHA2D06182g
PICPICST_73043(ERG5)
PGUPGUG_05085
SPAASPAPADRAFT_63544(ERG5)
LELLELG_05188
CALCAALFM_C702840CA(ERG5)
CTPCTRG_04983
COTCORT_0G04140
CDUCD36_72510
CTENCANTEDRAFT_115386
YLIYALI0A18062g
CLUCLUG_04246
CLUSA9F13_04g02805
CAURCJI97_001893
SLBAWJ20_75(ERG5)
PKZC5L36_0B12520
BNNFOA43_004793(ERG5)
BBRXBRETT_002777(ERG5)
OPAHPODL_01958
NCRNCU05278
NTENEUTE1DRAFT116692(NEUTE1DRAFT_116692)
SMPSMAC_01710
PANPODANSg6262 PODANSg7995
TTTTHITE_128412
MTMMYCTH_73679
CTHRCTHT_0029760
MGRMGG_06047
PPEIPpBr36_02869
TMNUCRPA7_7946
SSCKSPSK_00476
FGRFGSG_01959 FGSG_03686
FPUFPSE_01847 FPSE_09181
FVRFVEG_07284 FVEG_08786
FOXFOXG_04166 FOXG_09685
NHENECHADRAFT_72313 NECHADRAFT_72652
TRETRIREDRAFT_122166 TRIREDRAFT_58689
TRRM419DRAFT_141218 M419DRAFT_74177
MAWMAC_08636
MAJMAA_01109
CMTCCM_02962
PLJVFPFJ_00660 VFPFJ_08344
VALVDBG_00192 VDBG_00755
VDAVDAG_00367 VDAG_08476
CFJCFIO01_05732 CFIO01_13734
CHIGCH63R_04552 CH63R_14243
SAPOSAPIO_CDS4161 SAPIO_CDS7005
ELAUCREL1_11694
PFYPFICI_04403 PFICI_13034
SSLSS1G_09832
BFUBCIN_07g01120(Bcerg5)
MBEMBM_06688
PSCOLY89DRAFT_257563 LY89DRAFT_584410
GLZGLAREA_02023 GLAREA_06590
ANIAN4042.2
AFMAFUA_1G03950
ACTACLA_008020 ACLA_030640
NFINFIA_020740
AORAO090003000253 AO090003000954
ANGANI_1_350014(An01g02810) ANI_1_446094(An11g03230)
AFVAFLA_000720 AFLA_001436
ALUCAKAW2_20450S(ERG5_1) AKAW2_80298S(ERG5_2)
ACHEACHE_11570S(ERG5)
APUUAPUU_40308A(ERG5) APUU_60358A
PCSPc16g08330 Pc22g07520
PDPPDIP_21270
TMFPMAA_054960
TRGTRUGW13939_00550 TRUGW13939_08662
CIMCIMG_05009
CPWCPC735_068810
UREUREG_06546
PBLPAAG_11073(PAAG_00230)
PBNPADG_07898
ABEARB_03814
TVETRV_05093
AJEHCAG_06013
BGHBDBG_03201
PNOSNOG_06055
PTEPTT_19871
BZECOCCADRAFT_88203
BSCCOCSADRAFT_221542
BORCOCMIDRAFT_93916
AALTCC77DRAFT_991604
ZTRMYCGRDRAFT_55438(CYP-70)
PFJMYCFIDRAFT_49450
FFUCLAFUR5_02386
BCOMBAUCODRAFT_489380
NPAUCRNP2_2755
TMLGSTUM_00005882001
SPOSPAC19A8.04(erg5)
CNECNF03720
CNBCNBF1100
CNGCNAG_06644
CGICGB_F1500C
TMSTREMEDRAFT_40696
TASAA1Q1_00690
TVSTRAVEDRAFT_140384
DSQDICSQDRAFT_66936
PCOPHACADRAFT_246788
SHSSTEHIDRAFT_123767
HIRHETIRDRAFT_141009(cpm111)
PSQPUNSTDRAFT_59236
ADLAURDEDRAFT_109660
FMEFOMMEDRAFT_106824 FOMMEDRAFT_155371
GTRGLOTRDRAFT_53715
LBCLACBIDRAFT_187166(ERG5)
MRRMoror_12729
MOREE1B28_009893
CCICC1G_01671
SCMSCHCODRAFT_13845
ABPAGABI1DRAFT66440(AGABI1DRAFT_66440)
ABVAGABI2DRAFT189837(AGABI2DRAFT_189837)
CPUTCONPUDRAFT_94730
SLASERLADRAFT_459032
WSEWALSEDRAFT_69870
WICJ056_001310
UMAUMAG_00350
PFPPFL1_03644
MGLMGL_0310
MRTMRET_0994
MSYMMSY001_2710
 » show all
Reference
1  [PMID:8543054]
  Authors
Kelly SL, Lamb DC, Corran AJ, Baldwin BC, Parks LW, Kelly DE
  Title
Purification and reconstitution of activity of Saccharomyces cerevisiae P450 61,  a sterol delta 22-desaturase.
  Journal
FEBS Lett 377:217-20 (1995)
DOI:10.1016/0014-5793(95)01342-3
Reference
2  [PMID:8635732]
  Authors
Skaggs BA, Alexander JF, Pierson CA, Schweitzer KS, Chun KT, Koegel C, Barbuch R, Bard M
  Title
Cloning and characterization of the Saccharomyces cerevisiae C-22 sterol desaturase gene, encoding a second cytochrome P-450 involved in ergosterol biosynthesis.
  Journal
Gene 169:105-9 (1996)
DOI:10.1016/0378-1119(95)00770-9
  Sequence
[sce:YMR015C]
Reference
3  [PMID:16531502]
  Authors
Morikawa T, Mizutani M, Aoki N, Watanabe B, Saga H, Saito S, Oikawa A, Suzuki H, Sakurai N, Shibata D, Wadano A, Sakata K, Ohta D.
  Title
Cytochrome P450 CYP710A encodes the sterol C-22 desaturase in Arabidopsis and tomato.
  Journal
Plant Cell 18:1008-22 (2006)
DOI:10.1105/tpc.105.036012
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.19.41
IUBMB Enzyme Nomenclature: 1.14.19.41
ExPASy - ENZYME nomenclature database: 1.14.19.41
BRENDA, the Enzyme Database: 1.14.19.41

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