KEGG   ENZYME: 1.14.20.6
Entry
EC 1.14.20.6                Enzyme                                 
Name
flavonol synthase;
FLS (gene name)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
With 2-oxoglutarate as one donor, and the other dehydrogenated
Sysname
dihydroflavonol,2-oxoglutarate:oxygen oxidoreductase
Reaction(IUBMB)
a dihydroflavonol + 2-oxoglutarate + O2 = a flavonol + succinate + CO2 + H2O [RN:R07368]
Reaction(KEGG)
Substrate
dihydroflavonol [CPD:C15570];
2-oxoglutarate [CPD:C00026];
O2 [CPD:C00007]
Product
flavonol [CPD:C01495];
succinate [CPD:C00042];
CO2 [CPD:C00011];
H2O [CPD:C00001]
Comment
In addition to the desaturation of (2R,3R)-dihydroflavonols to flavonols, the enzyme from Citrus unshiu (satsuma mandarin) also has a non-specific activity that trans-hydroxylates the flavanones (2S)-naringenin and the unnatural (2R)-naringenin at C-3 to kaempferol and (2R,3R)-dihydrokaempferol, respectively [2]. Requires Fe2+.
History
EC 1.14.20.6 created 2004 as EC 1.14.11.23, transferred 2018 to EC 1.14.20.6
Pathway
ec00941  Flavonoid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K05278  flavonol synthase
Genes
ATHAT5G08640(FLS1) AT5G43935(FLS6) AT5G63580(FLS2) AT5G63590(FLS3) AT5G63595(FLS4) AT5G63600(FLS5)
ALY9300932 9300933 9300934 9301468 9302635 9309443 9316690 9317565
CRB17874951 17874978 17875062 17875365 17875649 17876064 17876240 17876394 17876899 17884061 17887601
CSAT104708215 104725029 104726949 104726979 104726980 104726983 104726984 104726985 104726986 104727463 104729053 104734897 104741256 104741513 104741522 104741531 104741542 104741553 104741561 104741572 104741592 104741603 104762497 104762498 104762499 104762501 104762503 104769085 104778112 104784343 109124872
EUSEUTSA_v10004662mg EUTSA_v10004668mg EUTSA_v10005710mg EUTSA_v10014054mg EUTSA_v10028827mg EUTSA_v10029534mg
BRP103837496 103847177 103855056 103855057 103873165 103873769 103873770
BNA106348278 106348289 106348297 106351881 106351882 106364152 106382906 106397936 106405224 106409586 106419433 106444537 111201386
BOE106313418 106316306 106320382 106328324 106332816 106341567
RSZ108806153 108812047 108814290 108825420 108856337 108856338 108859840 108861252
THJ104819356
CPAP110816514
CIT102618406 102626432 102629970
CICCICLE_v10003741mg CICLE_v10015856mg CICLE_v10026028mg
PVY116110546 116110548 116110558 116133801 116145683
MINC123199612 123199749 123199750 123202765 123202986 123206089 123206097 123218347
TCC18592290 18592292 18599277
GRA105770783 105780413 105780414
GHI107904987 107923058 107940452 107940456 107959505 107959507
GAB108452122 108457975 108459765
DZI111275455 111314969
EGR104428803 104444489 104450481 104450482
GMX100810937 100816829 778157(FLS1)
GSJ114380973 114412340 114414096
PVUPHAVU_004G052400g PHAVU_008G1660000
VRA106763888 106769199
VAR108337532 108345477
VUN114180972 114193841
CCAJ109804443 109808020 109816831
APRC113848076 113848139 113853130
MTR11415879 11415909 11435413 11435414
CAM101504604 101509377
PSAT127088203 127118888 127118889
LJALj1g3v0705350.1(Lj1g3v0705350.1) Lj1g3v0775780.1(Lj1g3v0775780.1) Lj1g3v1380920.1(Lj1g3v1380920.1) Lj5g3v1865700.1(Lj5g3v1865700.1)
ADU107458261 107465302
AIP107609791 107617070
AHF112702733 112712950 112766372 112777054
LANG109326685 109356468 109357523 109360027
FVE101295969 101296827
RCN112170431 112173037
PPER18788166 18788410 18789071 18792331
PMUM103326444 103332906 103332909 103332910
PAVI110753589 110753698 110754141
PDUL117613713 117613942 117628886 117628896
MDM103413102(FLS) 103441195 114821553 114821784
PXB103933697 103956721
ZJU107415994 107421006
MNT21396296 21403067 21406496 21406497 21406498
CSV101219245
CMO103488630
BHJ120075949
MCHA111011799
CMAX111486888 111489355
CMOS111444648 111464499
CPEP111780272 111809053
RCU8274085 8274087 8275129
JCU105639310
HBR110654972 110655043 110665573
MESC110606120 110607889 110609989
POP18097776 18109811 7458869 7495105
PEU105108614 105114941 105116982 105127840
PALZ118041763 118050782 118050795 118051939 118058366
JRE108981974 108982002 108988364 108991214 109012769 118349241
QSU112003934 112004171 112033039
QLO115959418 115963835 115973387 115993005
TWL120003005 120010136
VVI100232938(FLS1) 100232939(FLS1) 100243852(FLS2) 100249002(FLS3) 100267819(FLS5)
VRI117905961 117906026 117906419 117907623
SLY101249699
SPEN107004995
SOT102577717(FLS)
SSTN125844784
CANN107843086 107869735
NTA107794305(FLS1) 107814657(NtFLS)
NSY104236078
NTO104101386
NAU109237082
INI109175998
ITR116002506
SIND105155397 105176681
OEU111397470 111409089
EGT105974437
SSPL121795166
APAN127257191
HAN110878613 110878615
ECAD122587212
LSV111900153
CCAV112515207 112515212
DCR108203535 108221899
CSIN114257498 114257531 114283320 114314253
BVG104883093 104901038
SOE110778214 110795044
CQI110693494 110714529 110725334
NNU104596714 104608517 104609672 104609673
MING122068147 122072145 122075321 122076036 122076177
TSS122662359 122663411
PSOM113346311 113346474 113346476 113356202 113356203 113361278
OSA4330843
DOSAOs02t0767300-01(Os02g0767300)
OBR102712047
BDI100831956
ATS109764595 109768154 109784747
TDC119294168 119304118 119318079 119321892
TAES123089513 123098991 123108371 123131062 123134660 123141888
TUA125511422 125512561 125512649
LPER127305944 127319118 127336784
SBI8078926
ZMA100272992 103627785
SITA101780580
SVS117842212
PVIR120642963 120653872
PHAI112882371
PDA103712517
EGU105058232
MUS103969181 103969182 103977541 103995223
PEQ110019794
AOF109820207
NCOL116259344 116261229
ATR18425268 18425281 18426066
 » show all
Reference
1  [PMID:12180990]
  Authors
Wellmann F, Lukacin R, Moriguchi T, Britsch L, Schiltz E, Matern U
  Title
Functional expression and mutational analysis of flavonol synthase from Citrus unshiu.
  Journal
Eur J Biochem 269:4134-42 (2002)
DOI:10.1046/j.1432-1033.2002.03108.x
  Sequence
Reference
2  [PMID:12620339]
  Authors
Lukacin R, Wellmann F, Britsch L, Martens S, Matern U.
  Title
Flavonol synthase from Citrus unshiu is a bifunctional dioxygenase.
  Journal
Phytochemistry 62:287-92 (2003)
DOI:10.1016/S0031-9422(02)00567-8
Reference
3  [PMID:12782296]
  Authors
Martens S, Forkmann G, Britsch L, Wellmann F, Matern U, Lukacin R
  Title
Divergent evolution of flavonoid 2-oxoglutarate-dependent dioxygenases in parsley.
  Journal
FEBS Lett 544:93-8 (2003)
DOI:10.1016/S0014-5793(03)00479-4
  Sequence
Reference
4  [PMID:14570878]
  Authors
Turnbull JJ, Nakajima J, Welford RW, Yamazaki M, Saito K, Schofield CJ.
  Title
Mechanistic studies on three 2-oxoglutarate-dependent oxygenases of flavonoid biosynthesis: anthocyanidin synthase, flavonol synthase, and flavanone 3beta-hydroxylase.
  Journal
J Biol Chem 279:1206-16 (2004)
DOI:10.1074/jbc.M309228200
Other DBs
ExplorEnz - The Enzyme Database: 1.14.20.6
IUBMB Enzyme Nomenclature: 1.14.20.6
ExPASy - ENZYME nomenclature database: 1.14.20.6
BRENDA, the Enzyme Database: 1.14.20.6
CAS: 146359-76-4

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