KEGG   ENZYME: 1.14.99.44
Entry
EC 1.14.99.44               Enzyme                                 

Name
diapolycopene oxygenase;
crtP (ambiguous)
Class
Oxidoreductases;
Acting on paired donors, with incorporation or reduction of molecular oxygen;
Miscellaneous
Sysname
4,4'-diapolycopene,AH2:oxygen oxidoreductase (4,4'-hydroxylating)
Reaction(IUBMB)
4,4'-diapolycopene + 4 reduced acceptor + 4 O2 = 4,4'-diapolycopenedial + 4 acceptor + 6 H2O [RN:R09671]
Reaction(KEGG)
R09671;
(other) R07654 R09727 R09728
Substrate
4,4'-diapolycopene [CPD:C19797];
reduced acceptor [CPD:C00030];
O2 [CPD:C00007]
Product
4,4'-diapolycopenedial [CPD:C19798];
acceptor [CPD:C00028];
H2O [CPD:C00001]
Comment
Little activity with neurosporene or lycopene. Involved in the biosynthesis of C30 carotenoids such as staphyloxanthin. The enzyme oxidizes each methyl group to the hydroxymethyl and then a dihydroxymethyl group, followed by the spontaneous loss of water to give an aldehyde group.
History
EC 1.14.99.44 created 2011
Pathway
ec00906  Carotenoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K10210  diapolycopene oxygenase
Genes
MMT: Metme_3774
MDN: JT25_002365
MDH: AYM39_15930
MKO: MKLM6_3290
METL: U737_16970(crtI)
MPAD: KEF85_14155(crtI)
MPSY: CEK71_14805
KUS: B9G99_06335
KMA: B9H00_08720
MEA: Mex_1p3665(ctrI)
MDI: METDI4240(ctrI)
MEX: Mext_3436
MCH: Mchl_3745
MPO: Mpop_3632
MET: M446_3156
MOR: MOC_5734
META: Y590_16875
MMES: MMSR116_03635(crtI)
GOH: B932_2266
GTI: FXF46_11910(crtI) FXF46_15790(crtI)
ABG: Asbog_01465(crtI)
RMUC: FOB66_05790(crtI)
NTN: D5366_08600(crtI)
LCK: HN018_16745(crtI)
AOZ: HUE56_02795(crtI)
SKT: IGS68_00375(crtI)
BPU: BPUM_2690
BPUM: BW16_14510
BPUS: UP12_13760
BACW: QR42_13585
BBEV: BBEV_0577(crtP-1) BBEV_1363(crtP-2)
BALT: CFN77_14445(crtI)
BMQ: BMQ_2021(crtI) BMQ_2949
BMD: BMD_1977(crtI) BMD_2978
BMEG: BG04_4322(crtP) BG04_5481(crtI)
BPF: BpOF4_14565(crtP)
CGOT: J1899_01870(crtI)
OIH: OB2459
AFL: Aflv_2195
HHD: HBHAL_1313 HBHAL_2763(crtNb)
TAID: KS242_09280(crtI)
PPSR: I6J18_04190(crtI)
PSYH: D0S48_20355(crtI)
POF: GS400_07995(crtI)
MEKU: HUW50_18265(crtI)
RMF: D5E69_12340(crtI)
SAU: SA2351
SAV: SAV2564
SAW: SAHV_2548
SAM: MW2485
SAS: SAS2450
SAR: SAR2646
SAC: SACOL2579
SAE: NWMN_2464(crtI)
SAD: SAAV_2630
SUJ: SAA6159_02460(crtI)
SUK: SAA6008_02603(crtI)
SUC: ECTR2_2417(crtI)
SUQ: HMPREF0772_10625(crtI)
SUZ: MS7_2571(crtP)
SUG: SAPIG2615
SAUA: SAAG_00385
SAUS: SA40_2318
SAUU: SA957_2402
SAUG: SA268_2480
SAUV: SAI7S6_1019090(crtP)
SAUW: SAI5S5_1019030(crtP)
SAUX: SAI6T6_1019040(crtP)
SAUY: SAI8T7_1019070(crtP)
SAUF: X998_2547
SAB: SAB2437c
SAUB: C248_2622
SAUC: CA347_2640(crtP)
SAUR: SABB_01120(crtP)
SAUI: AZ30_13435
SAUD: CH52_06180
SAMS: NI36_12830
SHA: SH0488
SHH: ShL2_00389(crtI)
SXO: SXYL_00051(crtP)
SCAP: AYP1020_1735(crtP)
SARL: SAP2_00910(crtP)
SPIC: SAMEA4384060_0087(crtP)
SSH: NCTC13712_02520(crtP)
SSIM: SAMEA4384339_2370(crtP)
MLEN: H3V22_08605(crtI)
LGZ: NCTC10812_02741(crtP)
EAN: Eab7_0711
EAP: KB235_12905(crtI) KB235_14645(crtI)
EACE: KKI46_02915(crtI) KKI46_04105(crtI)
PMW: B2K_16040
PLUT: EI981_26715(crtI)
PBK: Back11_13210(crtP)
PBAC: HUB98_14510(crtI)
COHN: KCTCHS21_30250(crtP_1) KCTCHS21_30350(crtP_2)
SACA: FFV09_16680(crtI)
SIV: SSIL_3759
PDEC: H1Q58_03735(crtI) H1Q58_14400(crtI)
SPOS: DV702_13350(crtI)
PANC: E2636_00235(crtI)
PLAY: DNR44_006400(crtI) DNR44_016995(crtI)
KPUL: GXN76_05120(crtI)
ECAS: ECBG_01335
EMU: EMQU_0489
CRN: CAR_c19220(crtP)
JDA: BW727_102018(crtP)
RCA: Rcas_2535
CAU: Caur_2410
CAG: Cagg_2983
HAU: Haur_0609
ROO: G5S37_15655(crtI)
RBA: RB11943
PIR: VN12_20065(crtP)
RUL: UC8_21150(crtP)
RML: FF011L_10680(crtP)
ROL: CA51_34620(crtP)
AHEL: Q31a_22280(crtP)
RLC: K227x_30590(crtP)
PND: Pla175_40250(crtP)
PLM: Plim_2740
PEH: Spb1_35070(crtP)
PLS: VT03_32435(crtP)
PLH: VT85_17065(crtP)
GES: VT84_20055(carA2)
FTJ: FTUN_7583
ULI: ETAA1_31530(crtP)
TSPH: KIH39_01650(crtI)
PBOR: BSF38_05169(crtP)
AGV: OJF2_67080(crtP)
PHM: PSMK_03760(crtI)
HBS: IPV69_01380(crtI)
ACA: ACP_2301
ABAS: ACPOL_3674
CVL: J8C06_02330(crtI)
CHLO: J8C02_09335(crtI)
DYS: G7050_08290(crtI)
BLQ: L21SP5_01096(crtP)
PGIN: FRZ67_12085(crtI)
FLS: GLV81_02010(crtI)
LACS: H4075_02190(crtI)
SGN: SGRA_1067
PEJ: FYC62_10480(crtI)
PEK: FFJ24_025770(crtI)
PROE: H9L23_26270(crtI)
PEX: IZT61_17310(crtI)
MUH: HYN43_029955(crtI)
MGK: FSB76_11385(crtI)
MRUB: DEO27_031165(crtI)
MGOS: DIU38_019705(crtI)
MGIK: GO620_013835(crtI)
SPIB: G8759_08155(crtI)
STAE: HNV11_05550(crtI)
RUP: DTQ70_22340(crtI)
FAE: FAES_0082(crtI)
PSEZ: HME7025_02380(crtI)
RHOZ: GXP67_14360(crtI)
HSW: Hsw_4190
HYH: D3Y59_13295(crtI)
HYJ: FHG12_08555(crtI)
HRS: HER32_10695(crtI)
HBN: GUY19_13355(crtI)
NIB: GU926_12040(crtI)
ADD: HUW48_24355(crtI)
ASWU: HUW51_08650(crtI)
FLM: MY04_1513(crtI)
FLL: EI427_04945(crtI)
FYA: KMW28_08635(crtI)
FKA: KM029_05570(crtI)
FUV: JR347_10465(crtI)
MARE: EJ994_07520(crtI)
NDO: DDD_2394
NOJ: EJ995_11890(crtI)
POM: MED152_02575(crtI1)
SPON: HME9304_02555(crtI)
AQB: D1818_13185(crtI)
AQA: D1815_18000(crtI)
AQD: D1816_14625(crtI)
OCI: FEZ18_11935(crtI)
CAGG: HYG79_01990(crtI)
CHYD: H4K34_00090(crtI)
CTS: Ctha_0974
IAL: IALB_0396(crtI)
CPRV: CYPRO_2658
 » show all
Reference
1  [PMID:15850982]
  Authors
Mijts BN, Lee PC, Schmidt-Dannert C
  Title
Identification of a carotenoid oxygenase synthesizing acyclic xanthophylls: combinatorial biosynthesis and directed evolution.
  Journal
Chem Biol 12:453-60 (2005)
DOI:10.1016/j.chembiol.2005.02.010
  Sequence
[sav:SAV2564]
Reference
2  [PMID:15933032]
  Authors
Tao L, Schenzle A, Odom JM, Cheng Q
  Title
Novel carotenoid oxidase involved in biosynthesis of 4,4'-diapolycopene dialdehyde.
  Journal
Appl Environ Microbiol 71:3294-301 (2005)
DOI:10.1128/AEM.71.6.3294-3301.2005
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.14.99.44
IUBMB Enzyme Nomenclature: 1.14.99.44
ExPASy - ENZYME nomenclature database: 1.14.99.44
BRENDA, the Enzyme Database: 1.14.99.44

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