KEGG   ENZYME: 1.17.1.3
Entry
EC 1.17.1.3                 Enzyme                                 
Name
leucoanthocyanidin reductase;
leucocyanidin reductase
Class
Oxidoreductases;
Acting on CH or CH2 groups;
With NAD+ or NADP+ as acceptor
Sysname
(2R,3S)-catechin:NADP+ 4-oxidoreductase
Reaction(IUBMB)
(2R,3S)-catechin + NADP+ + H2O = 2,3-trans-3,4-cis-leucocyanidin + NADPH + H+ [RN:R06532]
Reaction(KEGG)
R06532;
(other) R06533 R06615
Substrate
(2R,3S)-catechin [CPD:C06562];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Product
2,3-trans-3,4-cis-leucocyanidin [CPD:C05906];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
The enzyme catalyses the synthesis of catechin, catechin-4beta-ol (leucocyanidin) and the related flavan-3-ols afzelechin and gallocatechin, which are initiating monomers in the synthesis of plant polymeric proanthocyanidins or condensed tannins. While 2,3-trans-3,4-cis-leucocyanidin is the preferred flavan-3,4-diol substrate, 2,3-trans-3,4-cis-leucodelphinidin and 2,3-trans-3,4-cis-leucopelargonidin can also act as substrates, but more slowly. NADH can replace NADPH but is oxidized more slowly.
History
EC 1.17.1.3 created 2003
Pathway
ec00941  Flavonoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K13081  leucoanthocyanidin reductase
Genes
CPAP110824010
CIT102621411
CICCICLE_v10033821mg
PVY116124900
MINC123213776 123215765
TCC18604056(LAR) 18610545
GRA105764571 105765625
GHI107931042 107934794 107946451 121224433
GAB108454436 108476741
DZI111290788 111316766 111318411
EGR104426102 104426103 104433307
GMX100786879(LAR1) 100795100
GSJ114370702 114402606
PVUPHAVU_007G102100g
VRA106771362
VAR108324973
VUN114190745
CCAJ109813851
APRC113869601 113873201
MTRMTR_1g093720
CAM101493305
LJALj2g3v1172450.1(Lj2g3v1172450.1)
ADU107491282
AIP107643784
AHF112750525 112802690
LANG109356072
FVE101306809
RCN112199978
PPER18789589
PMUM103341429
PAVI110744276 110754077
PDUL117616123 117627654
MDM103402727(LAR1) 103451680(LAR2)
PXB103929197 103938522 103947071
ZJU107418854 107429483
MNT112091008 21405124
RCU8269513
JCU105641976
HBR110649288 110666021
MESC110617547 110630746
POP7466920 7475620 7483928
PEU105113933 105123652
PALZ118037319 118039969 118047583
JRE108993261 108994581 109000235 109002691
QSU112024706 112036510
QLO115963778 115965626 115991055
TWL120001998 120008990
VVI100232982(LAR1) 100233112(LAR2)
VRI117911022
SPEN114077302
SOT102594738
SSTN125868902
NTA107793724 107815501
NSY104222673
NTO104107265
NAU109235276
OEU111369334
HAN110917901
CSIN114263727 114266906 114323746
BVG104908227
SOE110789492
CQI110697307 110726068
NNU104601230 104603047
MING122080354 122084111
TSS122641046 122663687
PSOM113273916 113277573
NCOL116259067 116262645 116262876 116262877 116262878
OSA4332311
DOSAOs03t0259400-00(Os03g0259400)
OBR102717501
BDI100839668
ATS109751108
TDC119288727 119293758 119320953
TAES123081808 123094982 123098003 123175387
TUA125553985 125553987
PDA103696132 103700891
EGU105041053 105048528
MUS103974395
AOF109834931
ATR18423736
 » show all
Reference
1  [PMID:8470799]
  Authors
Tanner GJ, Kristiansen KN.
  Title
Synthesis of 3,4-cis-[3H]leucocyanidin and enzymatic reduction to catechin.
  Journal
Anal Biochem 209:274-7 (1993)
DOI:10.1006/abio.1993.1119
Reference
2  [PMID:12788945]
  Authors
Tanner GJ, Francki KT, Abrahams S, Watson JM, Larkin PJ, Ashton AR
  Title
Proanthocyanidin biosynthesis in plants. Purification of legume leucoanthocyanidin reductase and molecular cloning of its cDNA.
  Journal
J Biol Chem 278:31647-56 (2003)
DOI:10.1074/jbc.M302783200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 1.17.1.3
IUBMB Enzyme Nomenclature: 1.17.1.3
ExPASy - ENZYME nomenclature database: 1.17.1.3
BRENDA, the Enzyme Database: 1.17.1.3
CAS: 776323-46-7

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