KEGG   ENZYME: 1.3.1.3
Entry
EC 1.3.1.3                  Enzyme                                 
Name
Delta4-3-oxosteroid 5beta-reductase;
3-oxo-Delta4-steroid 5beta-reductase;
5beta-reductase;
androstenedione 5beta-reductase;
cholestenone 5beta-reductase;
cortisone 5beta-reductase;
cortisone beta-reductase;
cortisone Delta4-5beta-reductase;
steroid 5beta-reductase;
testosterone 5beta-reductase;
Delta4-3-ketosteroid 5beta-reductase;
Delta4-5beta-reductase;
Delta4-hydrogenase;
4,5beta-dihydrocortisone:NADP+ Delta4-oxidoreductase;
3-oxo-5beta-steroid:NADP+ Delta4-oxidoreductase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With NAD+ or NADP+ as acceptor
Sysname
5beta-cholestan-3-one:NADP+ 4,5-oxidoreductase
Reaction(IUBMB)
(1) 5beta-cholestan-3-one + NADP+ = cholest-4-en-3-one + NADPH + H+ [RN:R02609];
(2) 17,21-dihydroxy-5beta-pregnane-3,11,20-trione + NADP+ = cortisone + NADPH + H+ [RN:R02893]
Reaction(KEGG)
Substrate
5beta-cholestan-3-one [CPD:C03091];
NADP+ [CPD:C00006];
17,21-dihydroxy-5beta-pregnane-3,11,20-trione
Product
cholest-4-en-3-one [CPD:C00599];
NADPH [CPD:C00005];
H+ [CPD:C00080];
cortisone [CPD:C00762]
Comment
The enzyme from human efficiently catalyses the reduction of progesterone, androstenedione, 17alpha-hydroxyprogesterone and testosterone to 5beta-reduced metabolites; it can also act on aldosterone, corticosterone and cortisol, but to a lesser extent [8]. The bile acid intermediates 7alpha,12alpha-dihydroxy-4-cholesten-3-one and 7alpha-hydroxy-4-cholesten-3-one can also act as substrates [9].
History
EC 1.3.1.3 created 1961 (EC 1.3.1.23 created 1972, incorporated 2005), modified 2005
Pathway
ec00120  Primary bile acid biosynthesis
ec00140  Steroid hormone biosynthesis
ec01100  Metabolic pathways
Orthology
K00251  3-oxo-5-beta-steroid 4-dehydrogenase
K22419  Delta4-3-oxosteroid 5beta-reductase
Genes
HSA6718(AKR1D1)
PTR472607(AKR1D1)
PPS100985077(AKR1D1)
GGO101127761(AKR1D1)
PON100456267(AKR1D1)
NLE100596284(AKR1D1)
MCC709863(AKR1D1)
MCF102146771(AKR1D1)
MTHB126951141
CSAB103226977(AKR1D1)
CATY105589641(AKR1D1)
PANU101005921(AKR1D1)
TGE112621691(AKR1D1)
RRO104656621(AKR1D1)
RBB108530143(AKR1D1)
TFN117073081(AKR1D1)
PTEH111524786(AKR1D1)
CJC100409275(AKR1D1)
SBQ101047690(AKR1D1)
CSYR103257301(AKR1D1)
MMUR105859116(AKR1D1)
LCAT123647674(AKR1D1)
OGA100951149(AKR1D1)
MMU208665(Akr1d1)
MCAL110296786(Akr1d1)
MPAH110316235(Akr1d1)
RNO192242(Akr1d1)
MCOC116098736(Akr1d1)
MUN110549234(Akr1d1)
CGE100770348(Akr1d1)
MAUA101844953(Akr1d1)
PLEU114687082(Akr1d1)
MORG121463361(Akr1d1)
MFOT126505259
AAMP119806254(Akr1d1)
NGI103726081(Akr1d1)
HGL101716388(Akr1d1)
CPOC100713034(Akr1d1)
CCAN109700596(Akr1d1)
DORD105989555(Akr1d1)
DSP122105925(Akr1d1)
NCAR124990892
OCU100008612(AKR1D1) 100343388
OPI101530477 101531043
TUP102473225(AKR1D1)
CFA482711(AKR1D1)
CLUD112650929 112667108(AKR1D1)
VVP112907014(AKR1D1) 112912510
VLG121488716(AKR1D1)
AML100483237(AKR1D1)
UMR103669050(AKR1D1)
UAH113264742(AKR1D1)
UAR123788904(AKR1D1)
ELK111152033
LLV125081360
MPUF101687850(AKR1D1)
ORO101385978(AKR1D1)
EJU114211725(AKR1D1)
ZCA113911446(AKR1D1)
MLX118021135(AKR1D1)
NSU110582789(AKR1D1)
FCA101100551(AKR1D1)
PYU121028274(AKR1D1)
PBG122488370(AKR1D1)
LRUF124524272
PTG102949203(AKR1D1)
PPAD109269068(AKR1D1)
AJU106970424(AKR1D1)
HHV120232950(AKR1D1)
BTA513855(AKR1D1)
BOM102264964(AKR1D1)
BIU109557677(AKR1D1)
BBUB102400967(AKR1D1)
CHX102170484(AKR1D1)
OAS101103641(AKR1D1)
ODA120877627(AKR1D1)
CCAD122438694(AKR1D1)
SSC100520813(AKR1D1)
CFR102510401(AKR1D1)
CBAI105063045(AKR1D1)
CDK105091623(AKR1D1)
VPC102545225(AKR1D1)
BACU102998601(AKR1D1)
LVE103071980(AKR1D1)
OOR101283232(AKR1D1)
DLE111177911(AKR1D1)
PCAD102983669(AKR1D1)
PSIU116759871(AKR1D1)
ECB100066919(AKR1D1)
EPZ103541705(AKR1D1)
EAI106829989(AKR1D1)
MYB102243036(AKR1D1) 102246475
MYD102756994 102775514(AKR1D1)
MMYO118665735 118665774 118666354
MLF102419360 102430403 102430721 102440785 102441086
MNA107544502(AKR1D1)
PKL118713464
HAI109388534(AKR1D1)
DRO112299914(AKR1D1)
SHON118989102(AKR1D1)
AJM119059566(AKR1D1)
PDIC114507959(AKR1D1)
PHAS123829666(AKR1D1)
MMF118620122(AKR1D1)
RFQ117018329(AKR1D1)
PALE102882463(AKR1D1)
PGIG120601113(AKR1D1)
PVP105291623(AKR1D1)
RAY107499059(AKR1D1)
MJV108401937(AKR1D1)
TOD119253801(AKR1D1)
SARA101553779(AKR1D1)
LAV100665738(AKR1D1)
TMU101348700
DNM101441310(AKR1D1)
MDO100022753(AKR1D1)
GAS123250738(AKR1D1)
SHR100926677(AKR1D1)
PCW110216322(AKR1D1)
OAA100076664(AKR1D1)
GGA418107(AKR1D1)
PCOC116226801(AKR1D1)
MGP100547967(AKR1D1)
CJO107316041(AKR1D1)
NMEL110394267(AKR1D1)
APLA101802488(AKR1D1)
ACYG106031015(AKR1D1)
AFUL116493651(AKR1D1)
TGU100228769(AKR1D1)
LSR110475223(AKR1D1)
SCAN103819697(AKR1D1)
PMOA120501669(AKR1D1)
OTC121332789(AKR1D1)
PRUF121364338(AKR1D1)
GFR102038244(AKR1D1)
FAB101820121(AKR1D1)
PHI102107003(AKR1D1)
PMAJ107204921(AKR1D1)
CCAE111937017(AKR1D1)
CCW104696483(AKR1D1)
CBRC103623384(AKR1D1)
ETL114056172(AKR1D1)
ZAB102075168(AKR1D1)
ACHL103799941(AKR1D1)
FPG101910289(AKR1D1)
FCH102047333(AKR1D1)
CLV102085311(AKR1D1)
EGZ104135569(AKR1D1)
NNI104012395(AKR1D1)
PLET104620912(AKR1D1)
PCRI104028119
ACUN113491106(AKR1D1)
TALA104367230(AKR1D1)
PADL103924517(AKR1D1)
AFOR103906493(AKR1D1)
ACHC115352443(AKR1D1)
HALD104315756
CCRI104167113
CSTI104557165
EHS104514908
CMAC104485944(AKR1D1)
MUI104545819
FGA104079106
GSTE104255773
LDI104349543
MNB103782512
OHA104338306(AKR1D1)
NNT104406352(AKR1D1)
DPUB104296550(AKR1D1)
PGUU104458967(AKR1D1)
ACAR104529203(AKR1D1)
AVIT104268300
CVF104288606(AKR1D1)
AAM106488268(AKR1D1)
AROW112974672(AKR1D1)
NPD112945631(AKR1D1)
TGT104567349(AKR1D1)
DNE112995034(AKR1D1)
SCAM104147396(AKR1D1)
ASN102377874(AKR1D1)
AMJ102564670(AKR1D1)
CPOO109316537(AKR1D1)
GGN109296149(AKR1D1)
PSS102453451(AKR1D1)
CMY102942570(AKR1D1)
CPIC101950885(AKR1D1)
TST117881635(AKR1D1)
CABI116825721(AKR1D1)
MRV120396071(AKR1D1)
ACS100555605(akr1d1)
PVT110082711(AKR1D1)
SUND121931010(AKR1D1)
PBI103055499(AKR1D1)
PMUR107291327(AKR1D1)
CTIG120316511(AKR1D1)
TSR106542905(AKR1D1)
PGUT117655403(AKR1D1)
VKO123028853(AKR1D1)
PMUA114605306(AKR1D1)
ZVI118091002(AKR1D1)
GJA107115496(AKR1D1)
STOW125435628(AKR1D1)
XLA399023(akr1d1.S) 734699(akr1d1.L)
XTR594997(akr1d1)
NPR108799286(AKR1D1)
RTEM120932839(AKR1D1)
BBUF121003676
BGAR122928538
IPU100528314(akr1d1)
PHYP113531734
SMEO124400790
TFD113645710
AMEX103027718 103036813
EEE113573542(akr1d1)
TRU101070818(akr1d1)
LCO104921014 104933494
NCC104955509 104966316
CGOB115010013 115028230
ELY117248912 117262373
EFO125883159 125888077
PLEP121950382 121961182
SLUC116038388 116039770
ECRA117938861 117948477
ESP116673051 116676518 116694088
PFLV114549980 114560328
GAT120809633 120817231
PPUG119196682 119210485
MSAM119894433 119896779
CUD121505584 121514445
ALAT119024852 119032114
MZE101470353 101485185 106675749 112430079
ONL100710661 109194203
OAU116311272 116328738
OLA101170970 101175316
OML112152470 112156489
XMA102229839 102235761
XCO114153943 114160926
XHE116736728 116737466
PRET103459507 103465917
PFOR103134490 103156136
PLAI106953618 106960935
PMEI106916617 106917221
GAF122826458 122835658
CVG107094041 107094558
CTUL119776217 119785771
GMU124883186 124883189 124883190 124883191
NFU107383989 107388423
KMR108240983 108250100
ALIM106521816 106529753
NWH119415859 119428234
AOCE111573167 111577765
MCEP124999848 125015397
CSEM103380316 103382893
POV109624271 109632831(akr1d1)
SSEN122766821 122776017
HHIP117757641 117763322
HSP118101558 124851056
LCF108883064 108890588
SDU111224733 111229638
SLAL111650358 111651988
XGL120793768 120798183
HCQ109523861 109529813
BPEC110164799 110168059
MALB109974454(akr1d1)
BSPL114857915 114862576
SASA100286547(akr1d1)
STRU115196975(akr1d1)
OTW112218178
OMY110493394
OGO124038526
ONE115134781(akr1d1)
SALP111962216(akr1d1)
SNH120066149
CCLU121531721
ELS105018484 105020260
SFM108925654 114910603
PKI111858589(akr1d1)
AANG118219304
LOC102690612(akr1d1)
PSPA121318520 121319578
ARUT117415834 117419641
LCM102361835(AKR1D1)
CMK103171668
RTP109937167
HRF124150563
ATHAT4G24220(VEP1)
ALY9303747
CRB17877518 17879957
CSAT104722573 104731036
EUSEUTSA_v10025425mg
BRP103834914 103861471 103861849
BNA106352270 106376025 106415732 106427862 106442739
BOE106306475 106306528 106312412
RSZ108820562 108820574 108843172 108848791 108848794
THJ104799795 104800325 104807087 104825749 104825751
CPAP110818782
CIT102615692 102616181 102620547 102623230 102624573 102627045 102627336 102627624 102628308 102629281 112499560
CICCICLE_v10001370mg CICLE_v10001374mg CICLE_v10001388mg CICLE_v10001393mg CICLE_v10001412mg CICLE_v10001414mg CICLE_v10001420mg CICLE_v10001422mg CICLE_v10001445mg CICLE_v100016291m CICLE_v100016292m CICLE_v10003867mg CICLE_v10003925mg CICLE_v10006945mg CICLE_v10013989mg
PVY116129046 116129047
MINC123203547 123228556 123230124
TCC18592015 18608051
GRA105769314 105769315 105795195 105797302
GHI107886961 107905623 107905624 107908857 107910310 107942462 107958170 107958171
GAB108453067 108453068 108461381 108484246
DZI111275787 111308999 111315455
EGR104447829
GMX100813467
GSJ114414755
PVUPHAVU_009G070100g
VRA106759746
VAR108330564
VUN114164335
CCAJ109798087
APRC113865348 113874127 113875000 113875001
MTR11419174 11420385 11422455 11429177 25489935 25489936 25489937
CAM101490393 101510608 105852198
LJALj0g3v0358119.1(Lj0g3v0358119.1)
ADU107491418 107495841 107495842
AIP107643987 107647756 107647759
AHF112697779 112697780 112734717 112750748 112756079 112756080 112757903 112802512
LANG109326718 109328348 109338953 109343868
FVE101293643 101299678 101305374
RCN112168846 112168847 112169759 112169760 112169761
PPER18768093 18789471 18789486 18789508
PMUM103320398 103320460
PAVI110744881 110744883 110744884 110744886 110747742 110748783 110761998 110767981
PDUL117618478 117636548
MDM103410710 103415423 103420085 103424664 103431404 103455922 114825100
PXB103945296 103950796 103950843 103950858
ZJU107415767 107416244 107420554 107433676
MNT112093364 21394609
CSV101204090
CMO103489839
BHJ120087114
MCHA111026111
CMAX111477228 111480841
CMOS111442928 111445997
CPEP111787529 111797206
RCU8280990 8288518
JCU105650747
HBR110648142 110656806 110658141
MESC110615860 110625289
POP18104808 18109150 18109482 18109483 7472673 7497815
PEU105120216 105120579 105120582 105124313 105128848 105128857 105137942
PALZ118034852 118036549 118036557 118036563 118036575 118036582 118050001
JRE108996072 109001182 109001183 109002733 109017840
QSU112009411 112016509 112016510 112016511 112016526 112016533
QLO115967592 115973930 115973931 115973932 115973934 115973942 115977957
TWL120008549 120010703
VVI100247199 100264565
VRI117907358 117911806
SLY101055557 101257813 101259882
SPEN107002486 107028301 107032160
SOT102581216 102585153 102589154 102593127
SSTN125859660 125863992 125865296 125865297
CANN107848268 107854378 107854386 107877750 124890083
NTA107759409 107769538 107770580 107792196 107805824 107816081
NSY104214621 104221871 104221872
NTO104090341 104095344
NAU109219824 109226813
INI109161500 109165002 109165003
ITR116029969 116031692 116032856 116033642 116033643 116033644
SIND105155583 105169475 105169477 105169487 105172681
OEU111375829 111379316 111394633 111411979 111411981
EGT105957495 105957496
SSPL121753539 121758211 121759028 121805627 121806177
HAN110885341 110908254 110918315
ECAD122591302 122595170 122595358 122595359 122595422
LSV111877205 111884723
CCAV112500760 112506394 112516316 112516647
DCR108198789 108203561 108228116
CSIN114291800 114291805 114300518 114316018
BVG104889979 104901635
SOE110777898 110801569
CQI110696025 110716584 110716984 110736212
NNU104607744 104611667
MING122073678
TSS122661247 122661248
PSOM113289204
NCOL116250088
OSA107275612 4333193 4343823
DOSAOs03t0422600-00(Os03g0422600) Os03t0436400-01(Os03g0436400) Os07t0601600-00(Os07g0601600) Os11t0351555-01(Os11g0351555)
OBR102714167 102722456
BDI100833464
ATS109748315 109748316 109766846 109768392
TDC119270602 119270606 119273200 119278842 119278848 119279384 119334202 119340010
TAES123059503 123063548 123063549 123063553 123063559 123066863 123072676 123080859 123080862 123151670 123159117 123167325
TUA125522107 125530461 125546250
SBI110432343 8057144 8063222 8063223
ZMA100272352 100381428 103636683
SITA101767985 101768386
SVS117838595 117838596
PVIR120648084 120648765 120651988 120684530 120691350
PHAI112873238 112873239 112874862 112874863 112902975
PDA103707365 103707777
EGU105032501 105032502 105058029 109506055
MUS103979849 103981863 103989009
DCT110115669
PEQ110027269 110035037
AOF109847720
ATR18437608
SMOSELMODRAFT_402259 SELMODRAFT_444825 SELMODRAFT_74145 SELMODRAFT_86439 SELMODRAFT_86462 SELMODRAFT_92604
PPP112274793
 » show all
Reference
1  [PMID:13376613]
  Authors
DORFMAN RI, FORCHIELLI E.
  Title
Separation of delta 4-5 alpha-hydrogenases from rat liver homogenates.
  Journal
J Biol Chem 223:443-8 (1956)
Reference
2  [PMID:13805063]
  Authors
BROWN-GRANT K, FORCHIELLI E, DORFMAN RI.
  Title
The delta4-hydrogenases of guinea pig adrenal gland.
  Journal
J Biol Chem 235:1317-20 (1960)
Reference
3
  Authors
Levy, H.R. and Talalay, P.
  Title
Enzymatic introduction of double bonds into steroid ring A.
  Journal
J Am Chem Soc 79:2658-2659 (1957)
Reference
4  [PMID:13416214]
  Authors
TOMKINS GM.
  Title
The enzymatic reduction of delta 4-3-ketosteroids.
  Journal
J Biol Chem 225:13-24 (1957)
Reference
5  [PMID:2278855]
  Authors
Sugimoto Y, Yoshida M, Tamaoki B.
  Title
Purification of 5 beta-reductase from hepatic cytosol fraction of chicken.
  Journal
J Steroid Biochem Mol Biol 37:717-24 (1990)
DOI:10.1016/0960-0760(90)90356-p
Reference
6  [PMID:3828348]
  Authors
Furuebisu M, Deguchi S, Okuda K.
  Title
Identification of cortisone 5 beta-reductase as delta 4-3-ketosteroid 5 beta-reductase.
  Journal
Biochim Biophys Acta 912:110-4 (1987)
DOI:10.1016/0167-4838(87)90253-6
Reference
7  [PMID:6736016]
  Authors
Okuda A, Okuda K.
  Title
Purification and characterization of delta 4-3-ketosteroid 5 beta-reductase.
  Journal
J Biol Chem 259:7519-24 (1984)
Reference
8  [PMID:11342103]
  Authors
Charbonneau A, The VL.
  Title
Genomic organization of a human 5beta-reductase and its pseudogene and substrate selectivity of the expressed enzyme.
  Journal
Biochim Biophys Acta 1517:228-35 (2001)
DOI:10.1016/S0167-4781(00)00278-5
  Sequence
[hsa:6718]
Reference
9  [PMID:7508385]
  Authors
Kondo KH, Kai MH, Setoguchi Y, Eggertsen G, Sjoblom P, Setoguchi T, Okuda KI, Bjorkhem I
  Title
Cloning and expression of cDNA of human delta 4-3-oxosteroid 5 beta-reductase and substrate specificity of the expressed enzyme.
  Journal
Eur J Biochem 219:357-63 (1994)
DOI:10.1111/j.1432-1033.1994.tb19947.x
  Sequence
[hsa:6718]
Other DBs
ExplorEnz - The Enzyme Database: 1.3.1.3
IUBMB Enzyme Nomenclature: 1.3.1.3
ExPASy - ENZYME nomenclature database: 1.3.1.3
BRENDA, the Enzyme Database: 1.3.1.3
CAS: 9029-08-7

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