KEGG   ENZYME: 1.3.3.8
Entry
EC 1.3.3.8                  Enzyme                                 

Name
tetrahydroberberine oxidase;
(S)-THB oxidase
Class
Oxidoreductases;
Acting on the CH-CH group of donors;
With oxygen as acceptor
Sysname
(S)-tetrahydroberberine:oxygen oxidoreductase
Reaction(IUBMB)
(S)-tetrahydroberberine + 2 O2 = berberine + 2 H2O2 [RN:R02880]
Reaction(KEGG)
R02880;
(other) R03722 R04703 R08795 R11748
Substrate
(S)-tetrahydroberberine [CPD:C03329];
O2 [CPD:C00007]
Product
berberine [CPD:C00757];
H2O2 [CPD:C00027]
Comment
The enzyme from Berberis sp. is a flavoprotein; that from Coptis japonica is not. (R)-Tetrahydroberberines are not oxidized.
History
EC 1.3.3.8 created 1990 (EC 1.5.3.8 created 1989, incorporated 1992)
Pathway
ec00950  Isoquinoline alkaloid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K22089  tetrahydroprotoberberine oxidase
Genes
PSOM: 113274966 113303200
Reference
1
  Authors
Amann, M., Nagakura, N. and Zenk, M.H.
  Title
(S)-Tetrahydroprotoberberine oxidase the final enzyme in protoberberine biosynthesis.
  Journal
Tetrahedron Lett 25:953-954 (1984)
Reference
2
  Authors
Okada, N., Shinmyo, A., Okada, H. and Yamada, Y.
  Title
Purification and characterization of (S)-tetrahydroberberine oxidase from cultured Coptis japonica cells.
  Journal
Phytochemistry 27:979-982 (1988)
Other DBs
ExplorEnz - The Enzyme Database: 1.3.3.8
IUBMB Enzyme Nomenclature: 1.3.3.8
ExPASy - ENZYME nomenclature database: 1.3.3.8
BRENDA, the Enzyme Database: 1.3.3.8
CAS: 114705-00-9

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