KEGG ENZYME: 1.4.7.1

ENZYME: 1.4.7.1

Entry

EC 1.4.7.1                  Enzyme

Name

glutamate synthase (ferredoxin);
ferredoxin-dependent glutamate synthase;
ferredoxin-glutamate synthase;
glutamate synthase (ferredoxin-dependent)

Class

Oxidoreductases;
Acting on the CH-NH2 group of donors;
With an iron-sulfur protein as acceptor

Sysname

L-glutamate:ferredoxin oxidoreductase (transaminating)

Reaction(IUBMB)

2 L-glutamate + 2 oxidized ferredoxin = L-glutamine + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+ (overall reaction) [RN:R00021];
(1a) L-glutamate + NH3 = L-glutamine + H2O [RN:R00256];
(1b) L-glutamate + 2 oxidized ferredoxin + H2O = NH3 + 2-oxoglutarate + 2 reduced ferredoxin + 2 H+ [RN:R10086]

Reaction(KEGG)

R00021 R00256 R10086

Substrate

L-glutamate [CPD:C00025];
oxidized ferredoxin [CPD:C00139];
NH3 [CPD:C00014];
H2O [CPD:C00001]

Product

L-glutamine [CPD:C00064];
2-oxoglutarate [CPD:C00026];
reduced ferredoxin [CPD:C00138];
H+ [CPD:C00080];
H2O [CPD:C00001];
NH3 [CPD:C00014]

Comment

Binds a [3Fe-4S] cluster as well as FAD and FMN. The protein is composed of two domains, one hydrolysing L-glutamine to NH3 and L-glutamate (cf. EC 3.5.1.2, glutaminase), the other combining the produced NH3 with 2-oxoglutarate to produce a second molecule of L-glutamate. The NH3 is channeled through a 24 A channel in the active protein. No hydrolysis of glutamine takes place without ferredoxin and 2-oxoglutarate being bound to the protein [5,6].

History

EC 1.4.7.1 created 1976, modified 2012

Pathway

ec00630

Glyoxylate and dicarboxylate metabolism

ec00910

Nitrogen metabolism

ec01120

Microbial metabolism in diverse environments

Orthology

K00284

glutamate synthase (ferredoxin)

Genes

ATH:	AT2G41220(GLU2) AT5G04140(GLU1)

ALY:	9309205 9315983

CRB:	17882431 17889161

CSAT:

104708708 104734430 104768604 104782609 104785266 104793031

EUS:	EUTSA_v10012425mg EUTSA_v10016134mg

BRP:	103847326 103857972 103865867

BNA:	106352787 106372673 106391644 106431110 125593008

BOE:	106317861 106335201 106342177

RSZ:	108817709 108854286

THJ:	104803262 104816414

CPAP:

110822870

CIT:

102609089

CIC:	CICLE_v10004137mg

PVY:

116132388

MINC:

123197667 123205484

TCC:

18598741

GRA:

105786202

GHI:	107921512 107921972

GAB:

108466881

DZI:	111293883 111311455

EGR:

104444178

GMX:	100809299 548016

GSJ:	114400728 114406643

PVU:	PHAVU_001G123900g

VRA:

106752554

VAR:

108328874

VUN:

114186821

CCAJ:

109815625

APRC:

113863103

MTR:

11415038

TPRA:

123893182

CAM:

101506279

PSAT:

127126429

LJA:	Lj1g3v4154900.1(Lj1g3v4154900.1) Lj1g3v4154900.2(Lj1g3v4154900.2) Lj1g3v4154900.3(Lj1g3v4154900.3)

ADU:

107492689

AIP:

107645553

AHF:	112695943 112756317

LANG:

109343289

PCIN:

129287538

FVE:

101314871

RCN:

112188077

PPER:

18770391

PMUM:

103341391

PAVI:

110758098

PDUL:

117633614

MDM:

114820893

MSYL:

126598854

PXB:

103928813

ZJU:	107433784 107435873

MNT:

21402329

CSAV:

115699366

CSV:

101214380

CMO:

103486888

BHJ:

120090125

MCHA:

111016634

CMAX:

111469981 111482225

CMOS:

111441624 111446589

CPEP:

111789977 111800589

RCU:

8270489

HBR:	110658224 110664043

MESC:

110622961

POP:	7458925 7495696

PEU:	105108000 105127577

PALZ:

118033263 118053033

JRE:	108995228 109011587

CILL:

122275774 122278315

QSU:

112004190

QLO:

115952760

TWL:	119992974 119998497

VVI:

100233082

VRI:

117920766

SLY:

101246028

SPEN:

107013679

SOT:

102600112

SSTN:

125857428

CANN:

107852264

NTA:	107781744 107793691

NSY:

104241431

NTO:

104118686

NAU:

109233296

INI:

109161932

ITR:

116024931

SIND:

105159899

OEU:	111365860 111379166

EGT:

105949319

SSPL:

121795818 121799482

SMIL:

131002714

APAN:

127256579

HAN:

110928568

ECAD:

122581218

LSV:

111891706

CCAV:

112512315

DCR:

108226274

CSIN:

114293314

RVL:

131319555

BVG:

104889481

SOE:

110777056

CQI:

110710572

ATRI:

130826142 130827301

MOF:

131168155

NNU:

104611350

MING:

122057294

TSS:

122668165

PSOM:

113309085 113320934

OSA:

4344164

DOSA:

Os07t0658400-01(Os07g0658400)

OBR:

102718939

OGL:

127779142

BDI:

100827937

ATS:

109733233

TDC:	119353479 119362636

TAES:

123043871 123051744 123187649

LPER:

127331782

LRD:	124670619 124670718

SBI:

8073135

ZMA:

542710

SITA:

101766581

SVS:

117843736

PVIR:

120662072 120694741

PHAI:

112879687

PDA:	103701654 103716926

EGU:	105049124 105057601

MUS:

103976157

ZOF:	122051435 122055660

DCT:

110101016

PEQ:

110034398

AOF:

109849057

NCOL:

116246228

ATR:

18444596

SMO:	SELMODRAFT_155359 SELMODRAFT_161275 SELMODRAFT_448225

PPP:	112284730 112286024

CRE:	CHLRE_12g514050v5

VCN:	VOLCADRAFT_127156(glu1)

MNG:	MNEG_13125

CSL:	COCSUDRAFT_22625

CVR:	CHLNCDRAFT_142154

APRO:

F751_5489

OLU:	OSTLU_51965

OTA:	OT_ostta14g01900

BPG:	Bathy12g01040

MIS:	MICPUN_63658(GLU)

MPP:	MICPUCDRAFT_70244(GLU)

CME:	CymeCp051(gltB)

GSL:	Gasu_40050 JL72_p025(gltB)

CCP:	CHC_1080(gltB)

PTI:	PHATRDRAFT_56605

FCY:	FRACYDRAFT_225787

TPS:	THAPSDRAFT_269900(glsF)

AAF:	AURANDRAFT_37118(FG)

EHX:	EMIHUDRAFT_225692

GTT:	GUITHDRAFT_88543

EBS:	ECTOBSL9_2331

GER:	KP004_06430(gltB)

GSUB:

KP001_08935(gltB)

GNT:	KP003_05915(gltB)

GPL:	M1B72_14475(gltB)

AORY:

AMOR_34870(gltB)

APAU:

AMPC_36480(gltB)

PCAY:

FRD00_24080(gltB)

BBAE:

FRD01_18100(gltB)

BHAI:

KJK41_06010(gltB)

HNZ:	P9989_20685(gltB)

MSEM:

GMB29_09955(gltB)

CTHU:

HUR95_04910(gltB)

SLMS:

MM221_01525(gltB)

PTHI:

NDS46_25040(gltB)

PDY:	QJQ58_27090(gltB)

CCHL:

FPL14_09895(gltB)

CHEB:

HH215_17895(gltB)

CINT:

HZF06_00990(gltB)

RGV:	NQ502_17400(gltB)

SVJ:	NQ490_13625(gltB)

LASA:

L9O85_08735(gltB)

PETR:

QKW49_11705(gltB)

BCRO:

NQ527_04160(gltB)

BLIQ:

INP51_06770(gltB)

BWX:	NQ550_06850(gltB)

BMAS:

LK422_05140(gltB)

CASP:

NQ535_15715(gltB)

SINT:

K5I26_15105(gltB)

WCP:	H9Q76_10615(gltB)

QDO:	H9Q78_13590(gltB)

WHJ:	H9Q79_04290(gltB)

MFOR:

NQ534_15145(gltB)

KIB:	RBB56_03475(gltB)

CBIL:

EUBC25_26330

EVN:	NQ558_06390(gltB)

ALAC:

JYE50_14545(gltB)

AHC:	JYE49_14795(gltB)

TMR:

Tmar_0631

THEP:

DYI95_002945

AMUO:

KWG62_00095(gltB)

ATQ:	GH723_08305

EUZ:	DVS28_a2831

SYN:	sll1499(gltB) sll1502(gltB)

SYZ:	MYO_130230(gltB) MYO_14470(gltB)

SYY:	SYNGTS_0442(gltB) SYNGTS_2989(gltB)

SYT:	SYNGTI_0442(gltB) SYNGTI_2988(gltB)

SYS:	SYNPCCN_0442(gltB) SYNPCCN_2987(gltB)

SYQ:	SYNPCCP_0442(gltB) SYNPCCP_2987(gltB)

SYJ:	D082_27210(glsF) D082_30770(gltB)

SYO:	C7I86_02300 C7I86_15650

SYC:	syc0650_c(glsF)

SYU:	M744_12285

SYF:	Synpcc7942_0890

SYD:	Syncc9605_0326

SYE:	Syncc9902_2016

SYG:

sync_0387

SYR:	SynRCC307_2164(gltS)

SYX:	SynWH7803_0385(gltS)

SYP:	SYNPCC7002_A2393(gltS)

CYA:	CYA_2704(gltS)

CYB:	CYB_1253(gltS)

SYNE:

Syn6312_2924

SYNP:

Syn7502_00626

SYNK:

KR100_03040

SYNR:

KR49_10635

SYND:

KR52_00805

SYH:	Syncc8109_0331(glsF)

SYNW:

SynWH8103_02451(glsF)

SYV:	AWQ23_11920

SYL:	AWQ21_12565

SYNC:

CB0101_10210(gltB)

SYNY:

BM449_08435

DSL:	Dacsa_3392

SYW:	SYNW2132(glsF)

CGC:	Cyagr_1859

CYI:	CBM981_1898(gltS)

PMA:	Pro_1668(glsF)

PMM:	PMM1512(glsF)

PMT:

PMT_1777

PMN:	PMN2A_1078

PMI:	PMT9312_1604

PMB:	A9601_17161(gltB)

PMC:	P9515_16921(gltB)

PMF:	P9303_23581(gltB)

PMG:	P9301_17041(gltB)

PMH:	P9215_17801(gltB)

PMJ:	P9211_16351(gltB)

PME:	NATL1_19531(gltB)

PRM:

EW15_2017

CMP:	Cha6605_3414 Cha6605_4807

AMR:	AM1_1662(gltB) AM1_6366

WNA:	KA717_33855 KA717_38915(gltB)

TSZ:	OOK60_10605(gltB) OOK60_13930

TVN:	NIES2134_111810(glsF)

SLW:	BRW62_01870

TEL:	tll1368(glsF)

THN:	NK55_02305(glsF)

THEC:

FFX45_09225

LEP:	Lepto7376_1007

LEN:	LEP3755_14620 LEP3755_25630

LET:	O77CONTIG1_00377(gltS_2) O77CONTIG1_04635(gltB_2)

LBO:	LBWT_33050 LBWT_36910

KOV:	K9N68_24780(gltB) K9N68_26565

LSC:	KIK02_11125(gltB) KIK02_23740(gltB)

LSK:	J5X98_13485(gltB) J5X98_15655(gltB)

HHG:	XM38_009140(gltS)

PSEU:

Pse7367_2837

PSER:

ABRG53_0383(gltB) ABRG53_2376(gltS)

PGX:	OA858_07755(gltB) OA858_12645(gltB)

THEU:

HPC62_07970(gltB) HPC62_18475(gltB)

TSIN:

OXH18_09335(gltB) OXH18_18125

GLP:	Glo7428_3738

GEN:	GM3709_3385 GM3709_950

GEE:	GM3708_2694 GM3708_514

CHON:

NIES4102_36890(glsF)

MAR:	MAE_07560(gltB) MAE_29110(glsF)

MPK:	VL20_163 VL20_5286

MIQ:	B5D77_15080 B5D77_21585

MVZ:	myaer102_05130(glsF) myaer102_42050(gltB)

CAN:	Cyan10605_0489

CSN:	Cyast_0608 Cyast_0917

CYL:	AA637_10340(gltB) AA637_11000

HAO:	PCC7418_1264 PCC7418_1589

ENN:	FRE64_12340(gltB)

CYU:	UCYN_03690

CYT:	cce_4353(glsF)

CWA:	CwatDRAFT_5417

CYP:	PCC8801_3730

CYH:	Cyan8802_3782

CYC:	PCC7424_1468 PCC7424_3654

CYJ:	Cyan7822_2992 Cyan7822_4149

CYN:	Cyan7425_0526

TER:

Tery_0466

ARP:	NIES39_J05540(glsF)

PAGH:

NIES204_16940 NIES204_23340(glsF)

PPSU:

NO713_01836(gltS) NO713_05437(gltB)

OXY:	HCG48_19975

MVAG:

D0A34_12415 D0A34_16000

LFS:	HFV01_04520(gltB)

MIC:	Mic7113_1425 Mic7113_6228

GEI:	GEI7407_2827

OAC:	Oscil6304_3505 Oscil6304_4177

ONI:	Osc7112_1580 Osc7112_2538

MPRO:

BJP34_31320

PHOR:

JWS08_08250(gltB) JWS08_11275(gltB)

PYH:	NEA10_01415(gltB)

CEP:	Cri9333_0783 Cri9333_3015

GVI:	glr1508(glsF)

GLJ:	GKIL_0118(gltS)

GML:	ISF26_17045

ANA:	alr4344(gltS)

NPU:	Npun_R3877

NOS:	Nos7107_2676

NOP:	Nos7524_4221

NON:	NOS3756_08790

NFL:	COO91_04534

NOE:	CLI64_22005

NSH:

GXM_03059

NED:	HUN01_14830

AVA:

Ava_1294

NAZ:

Aazo_0653

ANB:	ANA_C10135(gltS)

ACY:	Anacy_2495

AWA:	AA650_06545

ANN:	EH233_11960

CSG:	Cylst_5006

RSIN:

B6N60_00992

HBQ:	QI031_02670(gltB)

CALO:

Cal7507_2925

CALT:

Cal6303_1504

CALH:

IJ00_19245

CALN:

NIES2098_46520

RIV:	Riv7116_1068

FIS:	FIS3754_01900

NSPH:

BDGGKGIB_02156(gltS)

DOU:	BMF77_02710(gltS)

DFS:	HGD76_03835(gltB)

DHT:	NG743_20300

CCUR:

IAR63_15135

CRK:	L3I90_16415

AFLO:

HEQ12_18625

TOQ:	HCG51_11690(gltB)

NCN:	BZZ01_24715

CTHE:

Chro_3057 Chro_3459

PLP:	Ple7327_4235 Ple7327_4643

SCS:	Sta7437_2250 Sta7437_2290

STAN:

STA3757_00340 STA3757_02320(gltB)

GCY:	LQF76_09870

CEO:	ETSB_0993(glsF)

CER:	RGRSB_0946(glsF)

CEEC:

P3F56_04940(gltB)

RRS:	RoseRS_2985

RCA:

Rcas_1903

CAU:

Caur_3258

CHL:	Chy400_3517

CAG:

Cagg_0145

HAU:

Haur_4476

LEK:	hrd7_07070(gltB)

CAP:	CLDAP_26600(gltB)

KBS:	EPA93_24905(gltB)

THUG:

KNN16_03195(gltB)

TRO:

trd_1105

TRA:

Trad_0209

OPR:	Ocepr_1168

MHD:	Marky_1283

FGI:	OP10G_3032

VBH:	CMV30_08635

OLE:	K0B96_16910(gltB)

OBT:	OPIT5_11435

RUFI:

K0V07_15545(gltB)

PPHT:

GA004_01940(gltB)

ROO:	G5S37_25000(gltB)

LAMB:

KBB96_08710(gltB)

SOA:	G3M56_005025(gltB)

FPES:

NXS98_03635(gltB)

MFH:	MFUM_2397(gltB)

MEAP:

MTHMO_1987(gltS)

VBA:	IMCC26134_00205

GES:	VT84_11790(gltS)

GMS:	SOIL9_14000

TIM:	GMBLW1_42690

FTJ:

FTUN_8218

ULI:	ETAA1_22910(gltB)

TSPH:

KIH39_16360

MCAD:

Pan265_27950(gltB_2)

TAER:

GT409_07855(gltB)

LPRO:

PQO03_15725(gltB)

ACA:	ACP_2997(gltB)

ACM:	AciX9_1897

GMA:	AciX8_2097

GRW:	FTO74_06975

GRB:	GOB94_02530

TSA:	AciPR4_2050

TRS:	Terro_1714

TALB:

FTW19_14165

EDA:	GWR55_04770

EDG:	H7846_10995

ADIN:

H7849_08145(gltB)

CVL:	J8C06_04680(gltB)

CHLO:

J8C02_06370(gltB)

SCOR:

J3U87_09960(gltB)

GEX:	GETHOR_14040

MSEA:

METESE_00650

GAU:	GAU_3081(gltB)

GPH:	GEMMAAP_15705

GGR:	HKW67_20475(gltB)

LPAL:

LDL79_15180(gltB)

LUY:	QZH61_12435(gltB)

SRM:	SRM_01351(gltB)

CTS:

Ctha_0711

SCHV:

BRCON_1495

HDL:	HALDL1_06165

HHB:	Hhub_1568(gltB)

HNO:	LT974_02880(gltB)

HLU:	LT972_10785(gltB)

HSU:	HLASF_0085(gltA1)

HSF:	HLASA_0085(gltA1)

SALR:

FQU85_01415(gltB)

HALR:

EFA46_006790(gltB)

HJE:	HacjB3_13680

HDO:	MUK72_07660(gltB)

HALX:

M0R89_12695(gltB)

HAXZ:

M0R88_13050(gltB)

HAYC:

NGM10_04565(gltB)

HAXY:

NGM07_18015(gltB)

SALI:

L593_09320

SAIM:

K0C01_03750(gltB)

HARA:

AArcS_2470(gltB)

HMA:	rrnAC0169(gltB)

HHI:	HAH_0919(gltB)

HHN:	HISP_04735

HAB:	SG26_12425

HTA:	BVU17_04490

HALJ:

G9465_07175(gltB) G9465_07910(gltB)

HSIN:

KDQ40_08925(gltB)

NPH:	NP_1794A(gltB)

NMO:	Nmlp_2987(gltB)

NHO:	HWV23_13415(gltB)

NSAL:

HWV07_05345(gltB)

HALA:

Hrd1104_06280

HASV:

SVXHr_1984(gltB)

HMU:

Hmuk_3135

HALZ:

E5139_02915(gltB)

HALL:

LC1Hm_2478(gltS)

HAZP:

GBQ70_02915(gltB)

HALI:

BV210_03985

HSN:	DV733_03050 DV733_16480

HDS:	HSR122_1784(gltB) HSR122_2266(gltB3)

HRR:	HZS55_11310(gltB)

HPEL:

HZS54_09415(gltB)

HLT:	I7X12_01920(gltB)

SSAI:

N0B31_00390(gltB)

HAAD:

MW046_08420(gltB)

HWA:	HQ_1714A(gltB)

HWC:	Hqrw_1833(gltB)

HVO:	HVO_0869(gltB)

HME:	HFX_0844(gltS)

HGI:	ABY42_04180

HALE:

G3A49_04365(gltB)

HLS:	KU306_08970(gltB)

HLN:	SVXHx_0827(glts)

HBO:	Hbor_23510

HAQ:	DU484_08370

HAJ:	DU500_08410

HAER:

DU502_05770

HRA:	EI982_08020

HLM:	DV707_13265

HALM:

FCF25_11350(gltB)

HLA:

Hlac_0912

HALP:

DOS48_03595

HALB:

EKH57_07395

HEZZ:

EO776_04990

HALQ:

Hrr1229_002420(gltB)

HSS:	J7656_11845(gltB)

HSAI:

HPS36_02170(gltB)

HAZZ:

KI388_02545(gltB)

SRUB:

C2R22_04850

HAE:	halTADL_0125

HALN:

B4589_007465(gltB)

HALG:

HUG10_00530(gltB)

HALU:

HUG12_17205(gltB)

HMP:	K6T50_14680(gltB)

HRE:	K6T36_14635(gltB)

HRM:	K6T25_02120(gltB)

HACB:

Hbl1158_06740(gltB)

HDF:	AArcSl_1360(gltB)

HTU:

Htur_3198

HSAL:

JMJ58_12465(gltB)

HAKZ:

J0X25_03350(gltB)

NMG:	Nmag_2529(gltB)

HXA:	Halxa_3804

NAT:

NJ7G_2607

NPE:	Natpe_0471

NVR:	FEJ81_02705(gltB)

NPL:	FGF80_08195(gltB)

NAY:	HYG81_08095(gltB)

HALY:

HYG82_11285(gltB)

HJT:	DVR14_11645

HLO:	J0X27_05560(gltB)

NGE:	Natgr_3664

SAWL:

NGM29_09860(gltB)

HALV:

NGM15_01985(gltB)

NOU:	Natoc_2112

HLR:	HALLA_17405

HLC:	CHINAEXTREME00065

NAJ:	B1756_00660

NAG:	AArcMg_1525

NAN:	AArc1_2117

NARA:

QQ977_09830(gltB)

NBG:	DV706_09260

NAS:	GCU68_02815(gltB)

HDA:	BB347_10485

» show all

Reference

1 [PMID:24254054]

Authors

Galvan F, Marquez AJ, Vega JM

Title

Purification and molecular properties of ferredoxin-glutamate synthase from Chlamydomonas reinhardii.

Journal

Planta 162:180-7 (1984)
DOI:10.1007/BF00410216

Reference

2 [PMID:4423889]

Authors

Lea PJ, Miflin BJ.

Title

Alternative route for nitrogen assimilation in higher plants.

Journal

Nature 251:614-6 (1974)
DOI:10.1038/251614a0

Reference

3 [PMID:12069605]

Authors

Ravasio S, Dossena L, Martin-Figueroa E, Florencio FJ, Mattevi A, Morandi P, Curti B, Vanoni MA

Title

Properties of the recombinant ferredoxin-dependent glutamate synthase of Synechocystis PCC6803. Comparison with the Azospirillum brasilense NADPH-dependent enzyme and its isolated alpha subunit.

Journal

Biochemistry 41:8120-33 (2002)
DOI:10.1021/bi020083r

Reference

4 [PMID:10898944]

Authors

Navarro F, Martin-Figueroa E, Candau P, Florencio FJ

Title

Ferredoxin-dependent iron-sulfur flavoprotein glutamate synthase (GlsF) from the Cyanobacterium synechocystis sp. PCC 6803: expression and assembly in Escherichia coli.

Journal

Arch Biochem Biophys 379:267-76 (2000)
DOI:10.1006/abbi.2000.1894

Reference

5 [PMID:11967268]

Authors

van den Heuvel RH, Ferrari D, Bossi RT, Ravasio S, Curti B, Vanoni MA, Florencio FJ, Mattevi A

Title

Structural studies on the synchronization of catalytic centers in glutamate synthase.

Journal

J Biol Chem 277:24579-83 (2002)
DOI:10.1074/jbc.M202541200

Reference

6 [PMID:12818206]

Authors

van den Heuvel RH, Svergun DI, Petoukhov MV, Coda A, Curti B, Ravasio S, Vanoni MA, Mattevi A

Title

The active conformation of glutamate synthase and its binding to ferredoxin.

Journal

J Mol Biol 330:113-28 (2003)
DOI:10.1016/S0022-2836(03)00522-9

Other DBs

ExplorEnz - The Enzyme Database:

1.4.7.1

IUBMB Enzyme Nomenclature:

1.4.7.1

ExPASy - ENZYME nomenclature database:

1.4.7.1

BRENDA, the Enzyme Database:

1.4.7.1

CAS:	62213-56-3

DBGET integrated database retrieval system