KEGG   ENZYME: 1.5.3.10
Entry
EC 1.5.3.10                 Enzyme                                 
Name
dimethylglycine oxidase;
dmg (gene name);
N,N-dimethylglycine:oxygen oxidoreductase (demethylating)
Class
Oxidoreductases;
Acting on the CH-NH group of donors;
With oxygen as acceptor
Sysname
N,N-dimethylglycine,5,6,7,8-tetrahydrofolate:oxygen oxidoreductase (demethylating,5,10-methylenetetrahydrofolate-forming)
Reaction(IUBMB)
N,N-dimethylglycine + 5,6,7,8-tetrahydrofolate + O2 = sarcosine + 5,10-methylenetetrahydrofolate + H2O2 [RN:R01564]
Reaction(KEGG)
R01564
Substrate
N,N-dimethylglycine [CPD:C01026];
5,6,7,8-tetrahydrofolate [CPD:C00101];
O2 [CPD:C00007]
Product
sarcosine [CPD:C00213];
5,10-methylenetetrahydrofolate [CPD:C00143];
H2O2 [CPD:C00027]
Comment
A flavoprotein (FAD). The enzyme, characterized from the bacterium Arthrobacter globiformis, contains two active sites connected by a large "reaction chamber". An imine intermediate is transferred between the sites, eliminating the production of toxic formaldehyde. In the absence of folate the enzyme does form formaldehyde. Does not oxidize sarcosine. cf. EC 1.5.8.4, dimethylglycine dehydrogenase.
History
EC 1.5.3.10 created 1992, modified 2022
Pathway
ec00260  Glycine, serine and threonine metabolism
ec01100  Metabolic pathways
Orthology
K00309  dimethylglycine oxidase
Genes
MGRMGG_12421
PPEIPpBr36_02012
TMNUCRPA7_1978
FGRFGSG_03414
FPUFPSE_08388
FVRFVEG_10784
FOXFOXG_12340
NHENECHADRAFT_51705
MAWMAC_06835
MAJMAA_00906
CMTCCM_08027
PLJVFPFJ_09728
VDAVDAG_05053
CFJCFIO01_00388
CLUPCLUP02_17239
CHIGCH63R_09496
PFYPFICI_10893
ANIAN8654.2
AFMAFUA_8G06470
NFINFIA_099100
AORAO090023000142
ANGANI_1_1304134(An15g02590)
AFVAFLA_006506
ALUCAKAW2_60437S
ACHEACHE_20201S
APUUAPUU_10234S
PCSPc13g04270
PDPPDIP_84880
TRGTRUGW13939_11773
PNOSNOG_10285
PTEPTT_07671
BZECOCCADRAFT_109688
BSCCOCSADRAFT_199303
BORCOCMIDRAFT_83186
AALTCC77DRAFT_718683
ZTRMYCGRDRAFT_91876
PFJMYCFIDRAFT_81948
FFUCLAFUR5_13648
VITADP71_15950
VSTLVJ81_05585
PLEOOHA_1_00894(mlr_2)
APCHIMB59_00006330
BMQBMQ_1539
BMDBMD_1520
BMHBMWSH_3690
BMEGBG04_3831(dmg)
PBJVN24_07415
MSAMycsm_03756
MMIMMAR_4420(gcvT_2)
MMAEMMARE11_42450(gcvT_2)
MLIMULP_04640(gcvT_2)
MSHOMSHO_02100
MMCMmcs_2459
MKMMkms_2504
MJLMjls_2496
MGROFZ046_07905
MBAIMB901379_03869(gcvT_3)
MRFMJO55_15790
MGIMflv_3080
MSPMspyr1_23950
MCBMycch_3087
MVQMYVA_3366
MAUUNCTC10437_03331(gcvT_3)
MMAGMMAD_49080
MTYMTOK_13900
MPSCMPSYJ_32520
MARZMARA_42230
MPOFMPOR_36250
MMATMMAGJ_50970
MSEIMSEDJ_08720
ASDAS9A_0968 AS9A_1005
CVACVAR_2042
CTERA606_08170
CPRECsp1_08840(dmg)
CCYCSCMU_10070 SCMU_34040
NBRO3I_018395
NTPCRH09_17885
NOZDMB37_02150
NAHF5544_24870
NYALTV02_18390
RHARHA1_ro01815
ROPROP_14920
ROAPd630_LPD05975
RHBNY08_3778
RFAA3L23_02135(dmg_1)
RHSA3Q41_01248(dmg)
RKOJWS14_09300 JWS14_16740
ROZCBI38_23385
RPSKJWS13_36545
GPOGPOL_c02700
GORKTR9_1190
GOMD7316_03170(dmg)
GAMGII34_03040
GOILK459_08500
GHNMVF96_06420
GMGNWF22_10500
SRTSrot_2474
SMASAVERM_6951
SCBSCAB_6961
SVLStrvi_5720
SHYSHJG_2829
SHOSHJGH_2593
SRCM271_43595
SMALSMALA_0689
SSOII1A49_07040
SBHSBI_00369
SDVBN159_7405
SALSSLNWT_1480
SALUDC74_1378
SALLSAZ_07405
SVTSVTN_37030
STREGZL_07820
SLDT261_7397
SLCSL103_14825
SRWTUE45_01810(mlr_1)
SPAVSpa2297_05215
SCLFBB341_19095
SGSAVL59_25750
STSIA4E84_39335
SALWCP975_05110
SGMGCM10017557_69210
SCADDN051_31695
SPHWNFX46_27495
SLSSLINC_8171
SNRSNOUR_35730
SAUOBV401_07295
SSIAA7J05_30010
SPUNBFF78_40630
SGVB1H19_07005
SALFSMD44_01424
STROSTRMOE7_05000
SFKKY5_1140
SNZDC008_04090
SGEDWG14_07333(dmg)
SLKSLUN_37375
SGDELQ87_32780
SCYAEJ357_07090
SKACP970_37115
SGALCP966_05380
SPADDVK44_29080
SFYGFH48_33555
SAQUEJC51_07185
SSEOD0Z67_03935
SRIMCP984_35535
SPHVF9278_44460
SPACB1H29_30600
SCHACP983_39600
SCOECP976_02350
SSPBCP982_08295
SPLACP981_03560
SBROGQF42_41155
STUIGCM10017668_68780
SCYGS1361_07765(mlr1)
SDWK7C20_03430
SAUHSU9_002685
SROIIAG44_37905
SXNIAG42_07500
SHKJ2N69_05045
SANUK7396_32050
SMAOCAG99_15110
SGOBtest1122_25615
SINEKI385_05965
SLFJEQ17_38800
SDECL3078_42340
SAOVG3H79_31790
SDURM4V62_35110
ARTArth_3718
ARRARUE_c04530
ARHAHiyo8_21700
ARYATC04_01645
ARZAUT26_02035
ARUASPU41_10500
ARQBWQ92_12560 BWQ92_12630
ARNCGK93_02470
ARXARZXY2_3454
ACRYAC20117_06445 AC20117_10905
ARTHC3B78_00870
ARTPE5206_00090
ASUFMNQ99_15645
ARTYAHiyo4_00980 AHiyo4_02080
AAUAAur_0484
PUEFV140_02605
PNVJMY29_02220
ACHAchl_3463 Achl_3469
APNAsphe3_02290
PSULAU252_09435
PSNINIBR502771_07425 NIBR502771_07445
PSEYGU243_15190
GARAOZ07_16460
GCRGcLGCM259_2841
GLUF0M17_15520
GMINMP99_16080
GMYXH9_09775
KFVAS188_03570
KRSEQG70_16575
SATKSA2016_0189 SA2016_3446
NAEBHE16_09345
PAEYKUF55_01330
BSAUDWV08_05330
BLYA2T55_01520
BLINBLSMQ_0189
BCAUI6G59_04785
NGVCDO52_10660
STRREKD16_17065(mlr2)
SAIUJ4H86_06200
SROSros_3855
NOABKM31_33565
NOWGBF35_11145
NCXNocox_10415(mlr2)
NGNLCN96_11830
FRIFraEuI1c_1272
NAKEH165_03045
GOBGobs_2879
BSDBLASA_3541
MMARMODMU_2885
SENSACE_3149 SACE_4496 SACE_5616
SACGFDZ84_03475 FDZ84_32380
SVISvir_18850
AMDAMED_7548
AMNRAM_38785
AMMAMES_7436
AMZB737_7436
AOIAORI_6809
AJAAJAP_05070
AMQAMETH_4486
AMYCCU254_17875
AMYBBKN51_27315
AABA4R43_09625
AMYYYIM_25575(mlr4)
AORISD37_06610
AACDLWP59_25030
APRTMUY14_46680
AROONQK81_31955
PSEAWY02_22370
PSEEFRP1_20290
PSEQAD006_27705
PECQAD017_07300
PHHAFB00_18135
PAUTPdca_14210
PBROHOP40_33600
KALKALB_3171
KPHYAOZ06_00305
LEDBBK82_00765
AHMTL08_06780
ACTIUA75_06595
ACADUA74_06595
AHGAHOG_06315(mlr)
PMADBAY61_26185
SACEGIY23_13960 GIY23_15620
AMSAMIS_66490
ACTNL083_4087
AFSAFR_18435
ACTRAsp14428_12640
AEYCDG81_08445
RXYRxyl_0762
RRDRradSPS_0628
RUBGBA63_03815
RMARGBA65_09885
CAPCLDAP_16610
FLCKJS93_18725
HJEHacjB3_10225
HALREFA46_013930
HAADMW046_16085
HMApNG7370(gcvT1)
HHIHAH_5196(gcvT4)
HHNHISP_19155
HABSG26_17100
HTABVU17_16770
HALJG9465_20185
HSINKDQ40_19490
HDOMUK72_06290
HWAHQ_1761A(dmg2)
HWCHqrw_1891(dmg2)
HVOHVO_B0014(dmg1)
HGIABY42_15525
HALEG3A49_07115
HAQDU484_17270
HAJDU500_00310
HAERDU502_15680
HRAEI982_15310
HLMDV707_15295
HALBEKH57_15480
HALUHUG12_01570
HTUHtur_4706
HSALJMJ58_24020
HXAHalxa_1398
NVRFEJ81_00105
NAYHYG81_10490
NOUNatoc_3307
NASGCU68_06365
 » show all
Reference
1
  Authors
Mori, N., Kawakami, B., Tani, Y. and Yamada, H.
  Title
Purification and properties of dimethylglycine oxidase from Cylindrocarpon didymum M-1.
  Journal
Agric Biol Chem 44:1383-1389 (1980)
Reference
2  [PMID:11422368]
  Authors
Meskys R, Harris RJ, Casaite V, Basran J, Scrutton NS
  Title
Organization of the genes involved in dimethylglycine and sarcosine degradation in Arthrobacter spp.: implications for glycine betaine catabolism.
  Journal
Eur J Biochem 268:3390-8 (2001)
DOI:10.1046/j.1432-1327.2001.02239.x
  Sequence
Reference
3  [PMID:11926836]
  Authors
Basran J, Bhanji N, Basran A, Nietlispach D, Mistry S, Meskys R, Scrutton NS.
  Title
Mechanistic aspects of the covalent flavoprotein dimethylglycine oxidase of Arthrobacter globiformis studied by stopped-flow spectrophotometry.
  Journal
Biochemistry 41:4733-43 (2002)
DOI:10.1021/bi025519h
  Sequence
Reference
4  [PMID:12912903]
  Authors
Leys D, Basran J, Scrutton NS.
  Title
Channelling and formation of 'active' formaldehyde in dimethylglycine oxidase.
  Journal
EMBO J 22:4038-48 (2003)
DOI:10.1093/emboj/cdg395
  Sequence
Reference
5  [PMID:16964976]
  Authors
Basran J, Fullerton S, Leys D, Scrutton NS.
  Title
Mechanism of FAD reduction and role of active site residues His-225 and Tyr-259 in Arthrobacter globiformis dimethylglycine oxidase: analysis of mutant structure and catalytic function.
  Journal
Biochemistry 45:11151-61 (2006)
DOI:10.1021/bi061094d
Reference
6  [PMID:19369258]
  Authors
Tralau T, Lafite P, Levy C, Combe JP, Scrutton NS, Leys D.
  Title
An internal reaction chamber in dimethylglycine oxidase provides efficient protection from exposure to toxic formaldehyde.
  Journal
J Biol Chem 284:17826-34 (2009)
DOI:10.1074/jbc.M109.006262
Reference
7  [PMID:21188587]
  Authors
Casaite V, Poviloniene S, Meskiene R, Rutkiene R, Meskys R.
  Title
Studies of dimethylglycine oxidase isoenzymes in Arthrobacter globiformis cells.
  Journal
Curr Microbiol 62:1267-73 (2011)
DOI:10.1007/s00284-010-9852-6
Other DBs
ExplorEnz - The Enzyme Database: 1.5.3.10
IUBMB Enzyme Nomenclature: 1.5.3.10
ExPASy - ENZYME nomenclature database: 1.5.3.10
BRENDA, the Enzyme Database: 1.5.3.10
CAS: 37256-30-7

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