KEGG ENZYME: 1.5.99.14

ENZYME: 1.5.99.14

Entry

EC 1.5.99.14                Enzyme

Name

6-hydroxypseudooxynicotine dehydrogenase

Class

Oxidoreductases;
Acting on the CH-NH group of donors;
With unknown physiological acceptors

Sysname

1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one:acceptor 6-oxidoreductase (hydroxylating)

Reaction(IUBMB)

1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one + acceptor + H2O = 1-(2,6-dihydroxypyridin-3-yl)-4-(methylamino)butan-1-one + reduced acceptor [RN:R07514]

Reaction(KEGG)

R07514

Substrate

1-(6-hydroxypyridin-3-yl)-4-(methylamino)butan-1-one [CPD:C01297];
acceptor [CPD:C00028];
H2O [CPD:C00001]

Product

1-(2,6-dihydroxypyridin-3-yl)-4-(methylamino)butan-1-one [CPD:C15986];
reduced acceptor [CPD:C00030]

Comment

Contains a cytidylyl molybdenum cofactor [3]. The enzyme, which participates in the nicotine degradation pathway, has been characterized from the soil bacterium Arthrobacter nicotinovorans [1,2].

History

EC 1.5.99.14 created 2012

Pathway

ec00760

Nicotinate and nicotinamide metabolism

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K19185

6-hydroxypseudooxynicotine dehydrogenase subunit alpha

K19186

6-hydroxypseudooxynicotine dehydrogenase subunit beta

K19187

6-hydroxypseudooxynicotine dehydrogenase subunit gamma

Genes

PDIO:

PDMSB3_0230

RBK:	E0H22_06170

MOG:	MMB17_04665

ACUT:

MRB58_00995

BACA:

FAY30_04545 FAY30_22140 FAY30_22150

CFIR:

NAF01_14280

SCIA:

HUG15_03260

ROP:	ROP_27460(kdhL) ROP_27510(kdhM) ROP_27520(kdhB)

ROA:	Pd630_LPD07471 Pd630_LPD07476 Pd630_LPD07477

RGOR:

NMQ04_20060

GMG:	NWF22_15110

ARX:	ARZXY2_4556 ARZXY2_4561

PNV:	JMY29_20645 JMY29_20670 JMY29_20675

NCA:	Noca_0606 Noca_0607 Noca_0612

SAIU:

J4H86_13095

BLAP:

MVA48_02370

AACD:

LWP59_13125 LWP59_23005

AIH:	Aiant_73530(cutM)

SBAE:

DSM104329_02309(kdhA_1) DSM104329_05423(kdhA_6)

UAM:	UABAM_05846

» show all

Reference

Authors

Freudenberg, W., Konig, K. and Andreesen, J. R.

Title

Nicotine dehydrogenase from Arthrobacter oxidans: A molybdenum-containing hydroxylase.

Journal

FEMS Microbiology Letters 52:13-18 (1988)

Reference

2 [PMID:7815950]

Authors

Grether-Beck S, Igloi GL, Pust S, Schilz E, Decker K, Brandsch R

Title

Structural analysis and molybdenum-dependent expression of the pAO1-encoded nicotine dehydrogenase genes of Arthrobacter nicotinovorans.

Journal

Mol Microbiol 13:929-36 (1994)
DOI:10.1111/j.1365-2958.1994.tb00484.x

Reference

3 [PMID:16820521]

Authors

Sachelaru P, Schiltz E, Brandsch R

Title

A functional mobA gene for molybdopterin cytosine dinucleotide cofactor biosynthesis is required for activity and holoenzyme assembly of the heterotrimeric nicotine dehydrogenases of Arthrobacter nicotinovorans.

Journal

Appl Environ Microbiol 72:5126-31 (2006)
DOI:10.1128/AEM.00437-06

Other DBs

ExplorEnz - The Enzyme Database:

1.5.99.14

IUBMB Enzyme Nomenclature:

1.5.99.14

ExPASy - ENZYME nomenclature database:

1.5.99.14

UM-BBD (Biocatalysis/Biodegradation Database):

1.5.99.14

BRENDA, the Enzyme Database:

1.5.99.14

DBGET integrated database retrieval system