KEGG ENZYME: 1.7.1.4

ENZYME: 1.7.1.4

Entry

EC 1.7.1.4                  Enzyme

Name

nitrite reductase [NAD(P)H];
nitrite reductase (reduced nicotinamide adenine dinucleotide (phosphate));
assimilatory nitrite reductase (ambiguous);
nitrite reductase [NAD(P)H2];
NAD(P)H2:nitrite oxidoreductase;
nit-6 (gene name)

Class

Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With NAD+ or NADP+ as acceptor

Sysname

ammonia:NAD(P)+ oxidoreductase

Reaction(IUBMB)

NH3 + 3 NAD(P)+ + 2 H2O = nitrite + 3 NAD(P)H + 5 H+ [RN:R00787 R00789]

Reaction(KEGG)

R00787 R00789

Substrate

NH3 [CPD:C00014];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]

Product

nitrite [CPD:C00088];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]

Comment

An iron-sulfur flavoprotein (FAD) containing siroheme. The enzymes from the fungi Neurospora crassa [1], Emericella nidulans [2] and Candida nitratophila [4] can use either NADPH or NADH as electron donor. cf. EC 1.7.1.15, nitrite reductase (NADH).

History

EC 1.7.1.4 created 1961 as EC 1.6.6.4, transferred 2002 to EC 1.7.1.4, modified 2013

Pathway

ec00910

Nitrogen metabolism

Orthology

K17877

nitrite reductase (NAD(P)H)

Genes

QSU:	111999790 112036833

DHA:	DEHA2G21890g

BBRX:

BRETT_005342

OPA:	HPODL_02386

NCR:	NCU04720(nit-6)

NTE:	NEUTE1DRAFT87025(NEUTE1DRAFT_87025)

SMP:	SMAC_03842

PAN:	PODANSg3187

TTT:	THITE_2110175

MTM:	MYCTH_2306799

CTHR:

CTHT_0041190

MGR:

MGG_00634

PPEI:

PpBr36_08516

TMN:	UCRPA7_4641

SSCK:

SPSK_02946

FGR:	FGSG_08402

FPU:	FPSE_09019

FVR:	FVEG_02069

FOX:	FOXG_03192

NHE:	NECHADRAFT_77775

TRE:	TRIREDRAFT_69291

TRR:	M419DRAFT_13349

MAW:

MAC_03493

MAJ:

MAA_02214

PLJ:	VFPFJ_02751

VAL:	VDBG_08065

VDA:	VDAG_01561

CFJ:	CFIO01_11897

CHIG:

CH63R_08030

SAPO:

SAPIO_CDS10292

ELA:	UCREL1_8516

PFY:	PFICI_14163

SSL:	SS1G_04479

BFU:	BCIN_01g05790(BcniiA)

PSCO:

LY89DRAFT_578424 LY89DRAFT_582902

GLZ:	GLAREA_07493

ANI:

AN1007.2

AFM:	AFUA_1G12840

ACT:	ACLA_022630

NFI:	NFIA_012720

AOR:	AO090012000624

ANG:	ANI_1_804074(An08g05640)

AFV:	AFLA_002347

ALUC:

AKAW2_30507A

ACHE:

ACHE_30394S

APUU:

APUU_50779A

PCS:	Pc13g11420

PDP:	PDIP_28110

TMF:	PMAA_068100

TRG:	TRUGW13939_09493

PNO:	SNOG_06023

PTE:

PTT_08193

BZE:	COCCADRAFT_6545

BSC:	COCSADRAFT_162163

BOR:	COCMIDRAFT_37533

AALT:

CC77DRAFT_937904

ZTR:	MYCGRDRAFT_76215

PFJ:	MYCFIDRAFT_186832

FFU:	CLAFUR5_02626

BCOM:

BAUCODRAFT_476493

NPA:	UCRNP2_1294

TML:	GSTUM_00010229001

TVS:	TRAVEDRAFT_112003

DSQ:	DICSQDRAFT_182461

PCO:	PHACADRAFT_182112

SHS:	STEHIDRAFT_171639

HIR:	HETIRDRAFT_104673

PSQ:	PUNSTDRAFT_98111

GTR:	GLOTRDRAFT_45467

LBC:	LACBIDRAFT_291348

MRR:	Moror_15985

CCI:	CC1G_07228

SLA:	SERLADRAFT_449021(NiR)

UMA:	UMAG_11104

PFP:	PFL1_06434

PIF:	PITG_13013

PSOJ:

PHYSODRAFT_317690

» show all

Reference

1 [PMID:14426899]

Authors

NICHOLAS DJ, MEDINA A, JONES OT

Title

A nitrite reductase from Neurospora crassa.

Journal

Biochim Biophys Acta 37:468-76 (1960)
DOI:10.1016/0006-3002(60)90503-5

Reference

2 [PMID:4382427]

Authors

Pateman JA, Rever BM, Cove DJ

Title

Genetic and biochemical studies of nitrate reduction in Aspergillus nidulans.

Journal

Biochem J 104:103-11 (1967)
DOI:10.1042/bj1040103

Reference

Authors

Rivas, J., Guerrero, M. G., Paneque, A. and Losada, M.

Title

Characterization of the nitrate-reducing system of the yeast Torulopsis nitratophila.

Journal

Plant Sci Lett 1:105-113 (1973)

Reference

4 [PMID:4154942]

Authors

Lafferty MA, Garrett RH

Title

Purification and properties of the Neurospora crassa assimilatory nitrite reductase.

Journal

J Biol Chem 249:7555-67 (1974)

Reference

5 [PMID:126995]

Authors

Vega JM, Garrett RH

Title

Siroheme: a prosthetic group of the Neurospora crassa assimilatory nitrite reductase.

Journal

J Biol Chem 250:7980-9 (1975)

Reference

6 [PMID:150863]

Authors

Greenbaum P, Prodouz KN, Garrett RH

Title

Preparation and some properties of homogeneous Neurospora crassa assimilatory NADPH-nitrite reductase.

Journal

Biochim Biophys Acta 526:52-64 (1978)
DOI:10.1016/0005-2744(78)90289-9

Reference

7 [PMID:6457037]

Authors

Prodouz KN, Garrett RH

Title

Neurospora crassa NAD(P)H-nitrite reductase. Studies on its composition and structure.

Journal

J Biol Chem 256:9711-7 (1981)

Reference

8 [PMID:8096840]

Authors

Exley GE, Colandene JD, Garrett RH

Title

Molecular cloning, characterization, and nucleotide sequence of nit-6, the structural gene for nitrite reductase in Neurospora crassa.

Journal

J Bacteriol 175:2379-92 (1993)
DOI:10.1128/JB.175.8.2379-2392.1993

Sequence

[ncr:NCU04720]

Reference

9 [PMID:8798648]

Authors

Colandene JD, Garrett RH.

Title

Functional dissection and site-directed mutagenesis of the structural gene for NAD(P)H-nitrite reductase in Neurospora crassa.

Journal

J Biol Chem 271:24096-104 (1996)
DOI:10.1074/jbc.271.39.24096

Other DBs

ExplorEnz - The Enzyme Database:

1.7.1.4

IUBMB Enzyme Nomenclature:

1.7.1.4

ExPASy - ENZYME nomenclature database:

1.7.1.4

UM-BBD (Biocatalysis/Biodegradation Database):

1.7.1.4

BRENDA, the Enzyme Database:

1.7.1.4

CAS:

9029-29-2

DBGET integrated database retrieval system