KEGG   ENZYME: 1.7.1.4
Entry
EC 1.7.1.4                  Enzyme                                 
Name
nitrite reductase [NAD(P)H];
nitrite reductase (reduced nicotinamide adenine dinucleotide (phosphate));
assimilatory nitrite reductase (ambiguous);
nitrite reductase [NAD(P)H2];
NAD(P)H2:nitrite oxidoreductase;
nit-6 (gene name)
Class
Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With NAD+ or NADP+ as acceptor
Sysname
ammonia:NAD(P)+ oxidoreductase
Reaction(IUBMB)
NH3 + 3 NAD(P)+ + 2 H2O = nitrite + 3 NAD(P)H + 5 H+ [RN:R00787 R00789]
Reaction(KEGG)
R00787 R00789
Substrate
NH3 [CPD:C00014];
NAD+ [CPD:C00003];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Product
nitrite [CPD:C00088];
NADH [CPD:C00004];
NADPH [CPD:C00005];
H+ [CPD:C00080]
Comment
An iron-sulfur flavoprotein (FAD) containing siroheme. The enzymes from the fungi Neurospora crassa [1], Emericella nidulans [2] and Candida nitratophila [4] can use either NADPH or NADH as electron donor. cf. EC 1.7.1.15, nitrite reductase (NADH).
History
EC 1.7.1.4 created 1961 as EC 1.6.6.4, transferred 2002 to EC 1.7.1.4, modified 2013
Pathway
ec00910  Nitrogen metabolism
Orthology
K17877  nitrite reductase (NAD(P)H)
Genes
QSU111999790 112036833
DHADEHA2G21890g
BBRXBRETT_005342
OPAHPODL_02386
NCRNCU04720(nit-6)
NTENEUTE1DRAFT87025(NEUTE1DRAFT_87025)
SMPSMAC_03842
PANPODANSg3187
TTTTHITE_2110175
MTMMYCTH_2306799
CTHRCTHT_0041190
MGRMGG_00634
PPEIPpBr36_08516
TMNUCRPA7_4641
SSCKSPSK_02946
FGRFGSG_08402
FPUFPSE_09019
FVRFVEG_02069
FOXFOXG_03192
NHENECHADRAFT_77775
TRETRIREDRAFT_69291
TRRM419DRAFT_13349
MAWMAC_03493
MAJMAA_02214
PLJVFPFJ_02751
VALVDBG_08065
VDAVDAG_01561
CFJCFIO01_11897
CHIGCH63R_08030
SAPOSAPIO_CDS10292
ELAUCREL1_8516
PFYPFICI_14163
SSLSS1G_04479
BFUBCIN_01g05790(BcniiA)
PSCOLY89DRAFT_578424 LY89DRAFT_582902
GLZGLAREA_07493
ANIAN1007.2
AFMAFUA_1G12840
ACTACLA_022630
NFINFIA_012720
AORAO090012000624
ANGANI_1_804074(An08g05640)
AFVAFLA_002347
ALUCAKAW2_30507A
ACHEACHE_30394S
APUUAPUU_50779A
PCSPc13g11420
PDPPDIP_28110
TMFPMAA_068100
TRGTRUGW13939_09493
PNOSNOG_06023
PTEPTT_08193
BZECOCCADRAFT_6545
BSCCOCSADRAFT_162163
BORCOCMIDRAFT_37533
AALTCC77DRAFT_937904
ZTRMYCGRDRAFT_76215
PFJMYCFIDRAFT_186832
FFUCLAFUR5_02626
BCOMBAUCODRAFT_476493
NPAUCRNP2_1294
TMLGSTUM_00010229001
TVSTRAVEDRAFT_112003
DSQDICSQDRAFT_182461
PCOPHACADRAFT_182112
SHSSTEHIDRAFT_171639
HIRHETIRDRAFT_104673
PSQPUNSTDRAFT_98111
GTRGLOTRDRAFT_45467
LBCLACBIDRAFT_291348
MRRMoror_15985
CCICC1G_07228
SLASERLADRAFT_449021(NiR)
UMAUMAG_11104
PFPPFL1_06434
PIFPITG_13013
PSOJPHYSODRAFT_317690
 » show all
Reference
1  [PMID:14426899]
  Authors
NICHOLAS DJ, MEDINA A, JONES OT
  Title
A nitrite reductase from Neurospora crassa.
  Journal
Biochim Biophys Acta 37:468-76 (1960)
DOI:10.1016/0006-3002(60)90503-5
Reference
2  [PMID:4382427]
  Authors
Pateman JA, Rever BM, Cove DJ
  Title
Genetic and biochemical studies of nitrate reduction in Aspergillus nidulans.
  Journal
Biochem J 104:103-11 (1967)
DOI:10.1042/bj1040103
Reference
3
  Authors
Rivas, J., Guerrero, M. G., Paneque, A. and Losada, M.
  Title
Characterization of the nitrate-reducing system of the yeast Torulopsis nitratophila.
  Journal
Plant Sci Lett 1:105-113 (1973)
Reference
4  [PMID:4154942]
  Authors
Lafferty MA, Garrett RH
  Title
Purification and properties of the Neurospora crassa assimilatory nitrite reductase.
  Journal
J Biol Chem 249:7555-67 (1974)
Reference
5  [PMID:126995]
  Authors
Vega JM, Garrett RH
  Title
Siroheme: a prosthetic group of the Neurospora crassa assimilatory nitrite reductase.
  Journal
J Biol Chem 250:7980-9 (1975)
Reference
6  [PMID:150863]
  Authors
Greenbaum P, Prodouz KN, Garrett RH
  Title
Preparation and some properties of homogeneous Neurospora crassa assimilatory NADPH-nitrite reductase.
  Journal
Biochim Biophys Acta 526:52-64 (1978)
DOI:10.1016/0005-2744(78)90289-9
Reference
7  [PMID:6457037]
  Authors
Prodouz KN, Garrett RH
  Title
Neurospora crassa NAD(P)H-nitrite reductase. Studies on its composition and structure.
  Journal
J Biol Chem 256:9711-7 (1981)
Reference
8  [PMID:8096840]
  Authors
Exley GE, Colandene JD, Garrett RH
  Title
Molecular cloning, characterization, and nucleotide sequence of nit-6, the structural gene for nitrite reductase in Neurospora crassa.
  Journal
J Bacteriol 175:2379-92 (1993)
DOI:10.1128/JB.175.8.2379-2392.1993
  Sequence
[ncr:NCU04720]
Reference
9  [PMID:8798648]
  Authors
Colandene JD, Garrett RH.
  Title
Functional dissection and site-directed mutagenesis of the structural gene for NAD(P)H-nitrite reductase in Neurospora crassa.
  Journal
J Biol Chem 271:24096-104 (1996)
DOI:10.1074/jbc.271.39.24096
Other DBs
ExplorEnz - The Enzyme Database: 1.7.1.4
IUBMB Enzyme Nomenclature: 1.7.1.4
ExPASy - ENZYME nomenclature database: 1.7.1.4
UM-BBD (Biocatalysis/Biodegradation Database): 1.7.1.4
BRENDA, the Enzyme Database: 1.7.1.4
CAS: 9029-29-2

DBGET integrated database retrieval system