KEGG   ENZYME: 1.7.2.6
Entry
EC 1.7.2.6                  Enzyme                                 

Name
hydroxylamine dehydrogenase;
HAO (ambiguous);
hydroxylamine oxidoreductase (ambiguous);
hydroxylamine oxidase (misleading)
Class
Oxidoreductases;
Acting on other nitrogenous compounds as donors;
With a cytochrome as acceptor
Sysname
hydroxylamine:ferricytochrome-c oxidoreductase
Reaction(IUBMB)
(1) hydroxylamine + H2O + 4 ferricytochrome c = nitrite + 4 ferrocytochrome c + 5 H+ [RN:R10164];
(2) hydroxylamine + 3 ferricytochrome c = nitric oxide + 3 ferrocytochrome c + 3 H+ [RN:R10165]
Reaction(KEGG)
R10164 R10165
Substrate
hydroxylamine [CPD:C00192];
H2O [CPD:C00001];
ferricytochrome c [CPD:C00125]
Product
nitrite [CPD:C00088];
ferrocytochrome c [CPD:C00126];
H+ [CPD:C00080];
nitric oxide [CPD:C00533]
Comment
The enzymes from the nitrifying bacterium Nitrosomonas europaea [1,4] and the methylotrophic bacterium Methylococcus capsulatus [5] are hemoproteins with seven c-type hemes and one specialized P-460-type heme per subunit. The enzyme converts hydroxylamine to nitrite via an enzyme-bound nitroxyl intermediate [3]. While nitrite is the main product, the enzyme from Nitrosomonas europaea can produce nitric oxide as well [2].
History
EC 1.7.2.6 created 1972 as EC 1.7.3.4, part transferred 2012 to EC 1.7.2.6
Pathway
ec00910  Nitrogen metabolism
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K10535  hydroxylamine dehydrogenase
Genes
VFL: AL536_13820
PGH: FH974_09640
GHO: AL542_00990
SSE: Ssed_3758 Ssed_4366
SHL: Shal_1091
SWD: Swoo_4755
SKH: STH12_01223(hao1)
HALC: EY643_14770
MCA: MCA0956
METU: GNH96_08475
MAH: MEALZ_1830(hao)
MMAI: sS8_3996
NOC: Noc_0892
NHL: Nhal_1085
NWA: Nwat_0758
NTT: TAO_0474(haoA)
NTG: NSCAC_1127(hao)
THIP: N838_23495
SVA: SVA_2722
RFR: Rfer_0059
NEU: NE0962(hao2) NE2044(hao1) NE2339(hao3)
HCE: HCW_02425
HCM: HCD_03625
CFZ: CSG_11900
CGRA: CGRAC_2027
CHYO: CHH_0728
CGEO: CGEO_1057
CBLA: CBLAS_1476
CMUC: CMCT_0574
AMOL: AMOL_1575
HEBR: AEBR_2203
NAM: NAMH_1280
GME: Gmet_0142
GUR: Gura_1628
GLO: Glov_1044
GEM: GM21_0983
GBM: Gbem_3667
DEU: DBW_1324
DDE: Dde_0300
DFL: DFE_0004
DBA: Dbac_0217
DOL: Dole_1634
ADE: Adeh_1764
SFU: Sfum_2550
MSC: BN69_3242
SIL: SPOA0201
MAGQ: MGMAQ_1797
MGM: Mmc1_0550
MIN: Minf_2434(cccA)
MKC: kam1_2135
LCRE: Pla8534_10350(hao1)
PLH: VT85_05655(hao1)
PBOR: BSF38_05477(hao1)
AGV: OJF2_56510(hao1)
KST: KSMBR1_1220(hdh) KSMBR1_1764(hao_1) KSMBR1_2163(hao_2) KSMBR1_2670(hao_3) KSMBR1_3040(hao_4) KSMBR1_3397(hao_5) KSMBR1_3422(hao_6) KSMBR1_3442(hao_7) KSMBR1_3792(hao_8)
PCOR: KS4_29250(hao1)
ALUS: STSP2_00683(hao1)
IAL: IALB_0863
MRO: MROS_1112
NIO: NITINOP_0065(hao)
NJA: NSJP_2412
MOX: DAMO_2285 DAMO_2473(hao)
MBU: Mbur_0467
IHO: Igni_0955
IIS: EYM_05715
 » show all
Reference
1  [PMID:5758552]
  Authors
Rees MK.
  Title
Studies of the hydroxylamine metabolism of Nitrosomonas europaea. I. Purification of hydroxylamine oxidase.
  Journal
Biochemistry 7:353-66 (1968)
DOI:10.1021/bi00841a045
Reference
2  [PMID:497235]
  Authors
Hooper AB, Terry KR
  Title
Hydroxylamine oxidoreductase of Nitrosomonas. Production of nitric oxide from hydroxylamine.
  Journal
Biochim Biophys Acta 571:12-20 (1979)
DOI:10.1016/0005-2744(79)90220-1
Reference
3  [PMID:7117545]
  Authors
Hooper AB, Balny C.
  Title
Reaction of oxygen with hydroxylamine oxidoreductase of Nitrosomonas: fast kinetics.
  Journal
FEBS Lett 144:299-303 (1982)
DOI:10.1016/0014-5793(82)80658-3
Reference
4  [PMID:6289867]
  Authors
Lipscomb JD, Hooper AB.
  Title
Resolution of multiple heme centers of hydroxylamine oxidoreductase from Nitrosomonas. 1. Electron paramagnetic resonance spectroscopy.
  Journal
Biochemistry 21:3965-72 (1982)
DOI:10.1021/bi00260a010
Reference
5  [PMID:18650926]
  Authors
Poret-Peterson AT, Graham JE, Gulledge J, Klotz MG
  Title
Transcription of nitrification genes by the methane-oxidizing bacterium, Methylococcus capsulatus strain Bath.
  Journal
ISME J 2:1213-20 (2008)
DOI:10.1038/ismej.2008.71
Other DBs
ExplorEnz - The Enzyme Database: 1.7.2.6
IUBMB Enzyme Nomenclature: 1.7.2.6
ExPASy - ENZYME nomenclature database: 1.7.2.6
BRENDA, the Enzyme Database: 1.7.2.6
CAS: 9075-43-8

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