KEGG   ENZYME: 2.1.1.150
Entry
EC 2.1.1.150                Enzyme                                 
Name
isoflavone 7-O-methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:hydroxyisoflavone 7-O-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + a 7-hydroxyisoflavone = S-adenosyl-L-homocysteine + a 7-methoxyisoflavone [RN:R07375]
Reaction(KEGG)
R07375 > R06794 R07724
Substrate
S-adenosyl-L-methionine [CPD:C00019];
7-hydroxyisoflavone [CPD:C15615]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
7-methoxyisoflavone [CPD:C15616]
Comment
The enzyme from alfalfa can methylate daidzein, genistein and 6,7,4'-trihydroxyisoflavone but not flavones or flavanones.
History
EC 2.1.1.150 created 2003
Pathway
ec00943  Isoflavonoid biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K13262  isoflavone-7-O-methyltransferase
Genes
GMX100776336 100779638 100791825 100800021 100800912 100811609 100812688 100813225 100818724 100818947 100819257 100819796 100819853 100819957
GSJ114366990 114366991 114369283 114370383 114370796 114395435 114395780 114395852 114395926 114402360 114402638 114402878 114422730
PVUPHAVU_007G114000g PHAVU_008G032600g PHAVU_008G032700g
VRA106758582 106759051 106759056 106771418 111241498
VAR108323040 108323056
VUN114182971 114183378 114186064 114186125 114186248
CCAJ109795035 109802220 109802855 109802960 109818703
APRC113863219 113872610 113872612
MTRMTR_1g076930 MTR_1g076940 MTR_4g088340 MTR_4g088345 MTR_7g014510 MTR_7g014570
CAM101505606
LJALj1g3v3023790.1(Lj1g3v3023790.1)
ADU107463257 107464082 107486238 107486358 107486360 107486373 107486439 107486555 107486622 107486623 107486645 107489657
AIP107612696 107635412 107635414 107635489 107635490 107635608 107635609 107638164 107638166 107638167 107638188 107644738
AHF112707909 112709830 112740687 112740691 112740702 112740703 112740704 112740705 112744553 112744558 112744580 112744581 112744582 112744659 112744806 112744812 112744813 112744814 112751677 112772937 112794481 112794482 112794487 112794489 112794490 112794491 112794492 112794493 112797857 112797874 112797876 112798095 112798101 112798102 112798104 112801706 114927210 114927646
LANG109329173 109333424 109350006 109353780
SSPL121787256
 » show all
Reference
1
  Authors
Edwards, R. and Dixon, R.A.
  Title
Isoflavone O-methyltransferase activities in elicitor-treated cell suspension cultures of Medicago sativa.
  Journal
Phytochemistry 30:2597-2606 (1991)
Reference
2  [PMID:11006341]
  Authors
He XZ, Dixon RA.
  Title
Genetic manipulation of isoflavone 7-O-methyltransferase enhances biosynthesis of 4'-O-methylated isoflavonoid phytoalexins and disease resistance in alfalfa.
  Journal
Plant Cell 12:1689-702 (2000)
DOI:10.1105/tpc.12.9.1689
Reference
3  [PMID:8951042]
  Authors
He XZ, Dixon RA
  Title
Affinity chromatography, substrate/product specificity, and amino acid sequence analysis of an isoflavone O-methyltransferase from alfalfa (Medicago sativa L.).
  Journal
Arch Biochem Biophys 336:121-9 (1996)
DOI:10.1006/abbi.1996.0539
  Sequence
Reference
4  [PMID:9484461]
  Authors
He XZ, Reddy JT, Dixon RA
  Title
Stress responses in alfalfa (Medicago sativa L). XXII. cDNA cloning and characterization of an elicitor-inducible isoflavone 7-O-methyltransferase.
  Journal
Plant Mol Biol 36:43-54 (1998)
DOI:10.1023/A:1005938121453
  Sequence
Reference
5  [PMID:11752378]
  Authors
Liu CJ, Dixon RA.
  Title
Elicitor-induced association of isoflavone O-methyltransferase with endomembranes prevents the formation and 7-O-methylation of daidzein during isoflavonoid phytoalexin biosynthesis.
  Journal
Plant Cell 13:2643-58 (2001)
DOI:10.1105/tpc.010382
Reference
6  [PMID:11224575]
  Authors
Zubieta C, He XZ, Dixon RA, Noel JP.
  Title
Structures of two natural product methyltransferases reveal the basis for substrate specificity in plant O-methyltransferases.
  Journal
Nat Struct Biol 8:271-9 (2001)
DOI:10.1038/85029
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.150
IUBMB Enzyme Nomenclature: 2.1.1.150
ExPASy - ENZYME nomenclature database: 2.1.1.150
BRENDA, the Enzyme Database: 2.1.1.150
CAS: 136111-54-1

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