KEGG   ENZYME: 2.1.1.157
Entry
EC 2.1.1.157                Enzyme                                 
Name
sarcosine/dimethylglycine N-methyltransferase;
ApDMT;
sarcosine-dimethylglycine methyltransferase;
SDMT;
sarcosine dimethylglycine N-methyltransferase;
S-adenosyl-L-methionine:N,N-dimethylglycine N-methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:sarcosine(or N,N-dimethylglycine) N-methyltransferase [N,N-dimethylglycine(or betaine)-forming]
Reaction(IUBMB)
2 S-adenosyl-L-methionine + sarcosine = 2 S-adenosyl-L-homocysteine + betaine (overall reaction) [RN:R10061];
(1a) S-adenosyl-L-methionine + sarcosine = S-adenosyl-L-homocysteine + N,N-dimethylglycine [RN:R07243];
(1b) S-adenosyl-L-methionine + N,N-dimethylglycine = S-adenosyl-L-homocysteine + betaine [RN:R07244]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
sarcosine [CPD:C00213];
N,N-dimethylglycine [CPD:C01026]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
betaine [CPD:C00719];
N,N-dimethylglycine [CPD:C01026]
Comment
Cells of the oxygen-evolving halotolerant cyanobacterium Aphanocthece halophytica synthesize betaine from glycine by a three-step methylation process. The first enzyme, EC 2.1.1.156, glycine/sarcosine N-methyltransferase, leads to the formation of either sarcosine or N,N-dimethylglycine, which is further methylated to yield betaine (N,N,N-trimethylglycine) by the action of this enzyme. Both of these enzymes can catalyse the formation of N,N-dimethylglycine from sarcosine [3]. The reactions are strongly inhibited by S-adenosyl-L-homocysteine.
History
EC 2.1.1.157 created 2005, modified 2010
Pathway
ec00260  Glycine, serine and threonine metabolism
ec01100  Metabolic pathways
Orthology
K18897  sarcosine/dimethylglycine N-methyltransferase
K24071  glycine/sarcosine/dimethylglycine N-methyltransferase
Genes
SALHHMF8227_02086(gsmT)
LCDclem_04510
TIGTHII_1899
TMBThimo_1799
MPURMARPU_03460
TEETel_06785
THIPN838_15845
THIMKFB96_17235
HHAHhal_1677
HHKHH1059_14690
HHCM911_05555
EBSECTOBSL9_2475
TGRTgr7_1180
TKMTK90_0180
TNITVNIR_0235
TTITHITH_16325
TVRTVD_00880
SPIUSPICUR_05635
SROSBBH56_02800
NAXHC341_02605
WEZIC757_03180
TTCFOKN1_2396
THICTspCOW1_02650
SVASVA_2877
SALNSALB1_0940
BDLAK34_2248
BPYRABD05_28965
BUGBC1001_4028
BGPBGL_1c12680
BPLAbpln_1g12200
BPXBUPH_00610
PARABTO02_25155
APPCAP2UW1_2978 CAP2UW1_3536
GMEGmet_2958
GEOGeob_0241
GBNGEOBRER4_29660
DLIdnl_61890
HOHHoch_6747
MCIMesci_0387
MOPMesop_2713
MESWA9K65_001945
MHUAMCHK_0500
MJREB229_14570
MERDEB233_27850
NIYFQ775_18170
PLAPlav_2857
KMNHW532_17810
RJGCCGE525_02200
FILBN1229_v1_3117
FIYBN1229_v1_2803
RHZRHPLAN_16680
RBMTEF_00780 TEF_13940
LAPACP90_08120
LABRCHH27_01290
LABTFIU93_13755
LAGGB0E33_20175
ACUTMRB58_00815
REDroselon_03243
RIDRIdsm_00428 RIdsm_00793
MALUKU6B_49020 KU6B_49030
RHCRGUI_2989
HDHG5B40_07345
GAKX907_0698
AAYWYH_00220
ELQGa0102493_112273
SHUMSTHU_55140
SVCSTVA_54880
DEXHWD60_10300
MAGQMGMAQ_3233
NTHNther_0676
NCDACONDI_00157
MSAMycsm_00494
MMIMMAR_0861
MMAEMMARE11_08120
MLIMULP_00886
MPSEMPSD_09910
MSHOMSHO_26200
MKNMKAN_20335
MDXBTO20_00935
MSHGMSG_01046
MFJMFLOJ_23410
MSTOMSTO_27360
MSIMMSIM_14570
MNVMNVI_18340
MCOOMCOO_34280
MBAIMB901379_00735
MSEOMSEO_49750
MSPGF6B93_03465
MDUMDUV_36420
MPSCMPSYJ_29420
MPOFMPOR_13070
SVLStrvi_1838
SRCM271_16300
SMALSMALA_5460
SSOII1A49_30960
STREGZL_06301
SLDT261_1518
SGSAVL59_21380
SALWCP975_03075
SNRSNOUR_29320
SNWBBN63_13005
SAUOBV401_31165
SGVB1H19_02410
SALFSMD44_05398 SMD44_06865
SALJSMD11_6312
SFKKY5_7985c
SKACP970_42925
SBATG4Z16_08455
SSPBCP982_35650
SHUNDWB77_06607
SMOBJ7W19_32210
SGOBtest1122_02690
SAOVG3H79_06490 G3H79_13800
NPCKUV85_07820
NGVCDO52_05885
STRREKD16_20715
SAIUJ4H86_12575
ACTWF7P10_26560
SROSros_5201
SENSACE_3901
SACGFDZ84_36205
SVISvir_27050
PAUTPdca_14790
ACTIUA75_05195
ACADUA74_05190
PMADBAY61_22795
SACEGIY23_10125
MAUMicau_2701
MILML5_5694
MTUACSH63_11670
MICHFJK98_17165
MCABHXZ27_14045
CATICS0771_62280
NHYJQS43_05010
SNASnas_2966
AEYCDG81_09050
ATQGH723_14055
AQZKSP35_12380
ERZER308_18520
DSLDacsa_3636
HHGXM38_033970
HAOPCC7418_3511
ENNFRE64_13780
TERTery_2447 Tery_4388
MPROBJP34_03740 BJP34_03745 BJP34_29375
NPYNPRO_06340
AMUCPan181_00950
KSTKSMBR1_0461
PBUL21SP3_00980
PBPSTSP1_02232
ALUSSTSP2_01991
VBLL21SP4_02070
FBCFB2170_09406
PROCPtc2401_00945(rebM)
CTSCtha_2187
MMHMmah_0525
MHAZBHR79_00795
MEVMetev_0931
MPLMpal_2451
HALJG9465_06325
HTUHtur_0668
HSALJMJ58_05750
LOKILokiarch_49740 Lokiarch_52720
 » show all
Reference
1  [PMID:10896953]
  Authors
Nyyssola A, Kerovuo J, Kaukinen P, von Weymarn N, Reinikainen T
  Title
Extreme halophiles synthesize betaine from glycine by methylation.
  Journal
J Biol Chem 275:22196-201 (2000)
DOI:10.1074/jbc.M910111199
  Sequence
Reference
2  [PMID:11319079]
  Authors
Nyyssola A, Reinikainen T, Leisola M
  Title
Characterization of glycine sarcosine N-methyltransferase and sarcosine dimethylglycine N-methyltransferase.
  Journal
Appl Environ Microbiol 67:2044-50 (2001)
DOI:10.1128/AEM.67.5.2044-2050.2001
  Sequence
Reference
3  [PMID:12466265]
  Authors
Waditee R, Tanaka Y, Aoki K, Hibino T, Jikuya H, Takano J, Takabe T, Takabe T
  Title
Isolation and functional characterization of N-methyltransferases that catalyze betaine synthesis from glycine in a halotolerant photosynthetic organism Aphanothece halophytica.
  Journal
J Biol Chem 278:4932-42 (2003)
DOI:10.1074/jbc.M210970200
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.157
IUBMB Enzyme Nomenclature: 2.1.1.157
ExPASy - ENZYME nomenclature database: 2.1.1.157
BRENDA, the Enzyme Database: 2.1.1.157

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