KEGG   ENZYME: 2.1.1.314
Entry
EC 2.1.1.314                Enzyme                                 
Name
diphthine methyl ester synthase;
S-adenosyl-L-methionine:elongation factor 2 methyltransferase (ambiguous);
diphthine methyltransferase (ambiguous);
Dph5 (ambiguous)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] methyltransferase (diphthine methyl ester-[translation elongation factor 2]-forming)
Reaction(IUBMB)
4 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] = 4 S-adenosyl-L-homocysteine + diphthine methyl ester-[translation elongation factor 2] [RN:R11165]
Reaction(KEGG)
R11165
Substrate
S-adenosyl-L-methionine [CPD:C00019];
2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] [CPD:C04441]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
diphthine methyl ester-[translation elongation factor 2] [CPD:C21151]
Comment
This eukaryotic enzyme is part of the biosynthetic pathway of diphthamide. Different from the archaeal enzyme, which performs only 3 methylations, producing diphthine (cf. EC 2.1.1.98). The relevant histidine of elongation factor 2 is His715 in mammals and His699 in yeast. The order of the 4 methylations is not known.
History
EC 2.1.1.314 created 2015
Orthology
K00586  diphthine methyl ester synthase
Genes
HSA51611(DPH5)
PTR457062(DPH5)
PPS100976633(DPH5)
GGO101127053(DPH5)
PON100453387(DPH5)
NLE100586352(DPH5)
MCC712253(DPH5)
MCF102128354(DPH5)
CSAB103224358(DPH5)
CATY105590133(DPH5)
PANU101012769(DPH5)
TGE112624460(DPH5)
RRO104673263(DPH5)
RBB108519964(DPH5)
TFN117075442(DPH5)
PTEH111525764(DPH5)
CJC100415127(DPH5)
SBQ101042608(DPH5)
CSYR103252396(DPH5)
MMUR105862239(DPH5)
OGA100959084(DPH5)
MMU69740(Dph5)
MCAL110291208(Dph5)
MPAH110320467(Dph5)
RNO295394(Dph5)
MCOC116093671(Dph5)
MUN110551869(Dph5)
CGE100755934
PLEU114682444(Dph5)
NGI103741876(Dph5)
HGL101696986(Dph5)
CPOC100732624(Dph5)
CCAN109698072(Dph5)
DORD105997100(Dph5)
DSP122108752(Dph5)
NCAR124991537
OCU100345003(DPH5)
OPI101536409(DPH5)
TUP102477552(DPH5)
CFA612092(DPH5)
VVP112918478(DPH5)
VLG121486457(DPH5)
AML100481131(DPH5)
UMR103669323(DPH5)
UAH113254339(DPH5)
UAR123795150(DPH5)
ELK111143015
LLV125098429 125104965
MPUF101682000(DPH5)
ORO101369128(DPH5)
EJU114207936(DPH5)
ZCA113934849(DPH5)
MLX118019079(DPH5)
FCA101090957(DPH5)
PYU121027568(DPH5)
PBG122480843(DPH5)
PTG102958220(DPH5)
PPAD109271933(DPH5)
AJU106976419(DPH5)
HHV120226815(DPH5)
BTA508904(DPH5)
BOM102267176(DPH5)
BIU109556005(DPH5)
BBUB102408149(DPH5)
CHX102173522(DPH5)
OAS101115329(DPH5)
ODA120856789(DPH5)
CCAD122436627(DPH5)
SSC100157715(DPH5)
CFR102518705(DPH5)
CBAI105065363(DPH5)
CDK105084897(DPH5)
VPC102537923(DPH5)
BACU103000130(DPH5)
LVE103089744(DPH5)
OOR101285892(DPH5)
DLE111180109(DPH5)
PCAD102988442(DPH5) 112062807
PSIU116739752(DPH5)
ECB100050115(DPH5)
EPZ103550054(DPH5)
EAI106837770(DPH5)
MYB102248566(DPH5)
MYD102755928(DPH5)
MMYO118673470(DPH5)
MLF102436513(DPH5)
MNA107530684(DPH5)
PKL118727455(DPH5)
HAI109371355(DPH5)
DRO112313814(DPH5)
SHON118990626(DPH5)
AJM119038137 119054959(DPH5)
PDIC114488982(DPH5)
PHAS123804702(DPH5)
MMF118637891(DPH5)
RFQ117014701(DPH5)
PALE102884526(DPH5)
PGIG120607846(DPH5)
PVP105298160(DPH5)
RAY107504391(DPH5)
MJV108402213(DPH5)
TOD119236039(DPH5)
SARA101552034(DPH5)
LAV100660768(DPH5)
TMU101346258
DNM101417099(DPH5)
MDO100032968(DPH5)
GAS123235390 123243960(DPH5)
SHR100914997(DPH5)
PCW110217583(DPH5)
OAA100074886(DPH5)
GGA424463(DPH5)
PCOC116243533(DPH5)
MGP100549424(DPH5)
CJO107317334(DPH5)
NMEL110402399(DPH5)
APLA101793155(DPH5)
ACYG106039414(DPH5)
AFUL116491820(DPH5)
TGU100219750(DPH5)
LSR110477492(DPH5)
SCAN103814859(DPH5)
PMOA120506388(DPH5)
OTC121345232(DPH5)
PRUF121362196(DPH5)
GFR102036363(DPH5)
FAB101821538(DPH5)
PHI102114227(DPH5)
PMAJ107208069(DPH5)
CCAE111932728(DPH5)
CCW104695384(DPH5)
ETL114061651(DPH5)
ZAB102066581(DPH5)
FPG101921285(DPH5)
FCH102056137(DPH5)
CLV102086197(DPH5)
EGZ104134012(DPH5)
NNI104015521(DPH5)
ACUN113482993(DPH5)
TALA104360730(DPH5)
PADL103925092(DPH5)
ACHC115348770(DPH5)
AAM106499746(DPH5)
AROW112966828(DPH5)
NPD112951118(DPH5)
DNE112979777(DPH5)
ASN102388223(DPH5)
AMJ102573130(DPH5)
CPOO109307235(DPH5)
GGN109286888(DPH5)
PSS102462255(DPH5)
CMY102931019(DPH5)
CPIC101944042(DPH5)
TST117881427(DPH5)
CABI116839201(DPH5)
MRV120370732(DPH5)
ACS100562876(dph5)
PVT110076528(DPH5)
SUND121929331(DPH5)
PBI103049292(DPH5)
PMUR107302499(DPH5)
PGUT117677271(DPH5)
VKO123024529(DPH5)
PMUA114598700(DPH5)
ZVI118089424(DPH5)
GJA107107446(DPH5)
XLA380263(dph5.S)
XTR448611(dph5)
NPR108791464(DPH5)
RTEM120945617(DPH5)
BBUF120979469(DPH5)
BGAR122943107(DPH5)
DRE492514(dph5)
SRX107715097 107757489(dph5)
SANH107669825
SGH107588067(dph5) 107592713
CCAR109047508
CAUA113040132(dph5)
PPRM120486777(dph5)
IPU108271146(dph5)
PHYP113536511(dph5)
SMEO124391159(dph5)
TFD113650737(dph5)
AMEX103037464(dph5)
EEE113574556(dph5)
TRU101065858(dph5)
TNGGSTEN00008282G001
LCO104936355(dph5)
NCC104954007(dph5)
ELY117253951(dph5)
PLEP121940894(dph5)
SLUC116059029(dph5)
PFLV114561013(dph5)
GAT120824138(dph5)
PPUG119216181(dph5)
MSAM119888720 119889122
CUD121511893(dph5)
MZE101480932(dph5)
ONL100691910(dph5)
OAU116320806(dph5)
OLA101171985(dph5)
OML112136738(dph5)
XMA102236269(dph5)
XCO114141461(dph5)
XHE116726233(dph5)
PRET103463161(dph5)
PFOR103150015(dph5)
PLAI106954343(dph5)
PMEI106922598(dph5)
GAF122838560(dph5)
CVG107094698(dph5)
CTUL119784589(dph5)
GMU124874003(dph5)
NFU107393084(dph5)
KMR108248681(dph5)
ALIM106525066(dph5)
NWH119430474(dph5)
AOCE111583774(dph5)
CSEM103398551(dph5)
POV109644652(dph5)
SSEN122780969(dph5)
HHIP117759848(dph5)
LCF108874223(dph5)
SDU111236192(dph5)
SLAL111668215(dph5)
XGL120790601(dph5)
HCQ109506828(dph5)
MALB109965234(dph5)
SASA106573967(dph5) 106613590
OTW112250700
OMY110524102(dph5)
OGO123995730(dph5) 123997364
ONE115108141(dph5)
SALP111952694(dph5) 111975882
SNH120054002(dph5) 120054659
ELS105011378(dph5)
SFM108919061(dph5)
PKI111844640(dph5)
AANG118225790(dph5)
LOC102698591(dph5)
PSPA121326539(dph5)
ARUT117417302(dph5)
LCM102345453(DPH5)
CMK103188848(dph5)
RTP109915341(dph5)
BFO118432428
BBEL109475271
CIN100181968
SCLV120348207
SPU577892
APLC110977432
SKO100366716
DMEDmel_CG31289(Dph5)
DER6555113
DSE6607456
DSIDsimw501_GD18446(Dsim_GD18446)
DYADyak_GE24024(Dyak_Dph5)
DAN6498942
DSR110188269
DPODpse_GA16151
DPE6594873
DMN108156735
DWI6647360
DGR6567370
DMODmoj_GI10523
DAZ108616603
DNV108653623
DHE111595896
DVI6629689
CCAT101453064
BOD106616144
MDE101896586
SCAC106083398
LCQ111684683
AGAAgaP_AGAP004412
ACOZ120950438
AARA120895999
AAG5576116
AALB109403854 115264229
CQUCpipJ_CPIJ008675
CNS116346963
AME412557
ACER107998482
ALAB122715034
BIM100747247
BBIF117213118
BVK117232627
BVAN117156320
BTER100646946
BPYO122567498
CCAL108624624
OBB114880580
MGEN117221319
NMEA116423853
CGIG122398977
SOC105197794
MPHA105837618
AEC105152429
ACEP105626466
PBAR105427617
VEM105559747
HST105184193
DQU106750767
CFO105254164
FEX115237380
LHU105668887
PGC109857302
OBO105277059
PCF106790251
PFUC122517591
VPS122638173
NVI100120480
CSOL105367362
TPRE106651955
MDL103572391
CGLO123275103
FAS105268572
DAM107036001
AGIF122854429
CCIN107264162
DPA109537206
SOY115887322
ATD109598953
CSET123308655
AGB108914858
LDC111506996
NVL108567934
APLN108739515
PPYR116160474
OTU111423904
BMOR101746700
BMAN114240393
MSEX115443394
DPLKGM_202307
BANY112044307
PMAC106721558
PPOT106101098
PXU106120183 106127380 106128886
PRAP110997434
ZCE119828645
HAW110373393
TNL113504715
SLIU111350253
OFU114352254
PXY105382354
API100160206
DNX107166759
RMD113556934
BTAB109032879
DCI103506226 103506253
CLEC106669101
HHAL106690655
NLU111061408
PHUPhum_PHUM004200
FOC113206276
ZNE110831424
CSEC111863378
FCD110859232
DPXDAPPUDRAFT_331033
DMK116934882
PVM113811958
PJA122260754
PCHN125042568
PTRU123503472
EAF111713117
ISCIscW_ISCW015932
DSV119453817
RSAN119399618
RMP119175568 119179931
VDE111244279
VJA111269643
TUT107360805
DPTE113788857
DFR124498003
CSCU111634931
PTEP107451453
SDM118192405
CELCELE_B0491.7(B0491.7)
CBRCBG_02918
BMYBM_BM11348(Bm11348)
LOALOAG_02159
NAINECAME_15246
TSPTsp_10674
HROHELRODRAFT_169662
LGILOTGIDRAFT_213693
PCAN112574548
BGT106068956
GAE121375442
HRF124116728
HRJ124255685
CRG105329665 117684754
MYI110440663
PMAX117325476
MMER123525490
OBI106876766
OSN115217989 115227937
LAK106177069
SMMSmp_025570.1
OVIT265_03076
EGLEGR_02199
NVE5517180
EPA110255079
ATEN116293077
ADF107327155 107330171 107330804 107358146
AMIL114974404
PDAM113683056
SPIS111331566
DGT114515703
XEN124440175
HMG105845166
AQU100639400
ATHAT4G31790
ALY9305383
CRB17879784
CSAT104717011 104721658 104730135
EUSEUTSA_v10025950mg
BRP103851539
BNA106354744 106372680 106375398
BOE106309997 106324828
RSZ108822679
THJ104825772
CPAP110813746
CIT102624591
CICCICLE_v10002090mg
PVY116141785
MINC123203256 123228828
TCC18589955
GRA105790074
GHI107938393 121220142
GAB108467847
DZI111301173 111303931
EGR104448830
GMX100784092 100787586
GSJ114401518 114419736
PVUPHAVU_001G002400g
VRA106767046
VAR108341879
VUN114176477
CCAJ109805078
APRC113860093
MTRMTR_1g107520 MTR_3g113750 MTR_3g114210
CAM101492091
LJALj5g3v1807020.1(Lj5g3v1807020.1) Lj5g3v1807020.2(Lj5g3v1807020.2)
ADU107460175 107465594
AIP107609938 107635861
AHF112705640 112706912 112722746 112765890
LANG109338598
FVE101310189
RCN112171856
PPER18792326
PMUM103321264
PAVI110771090
PDUL117616530
MDM103428613 103450379
PXB103927928
ZJU107408931 107435849
MNT21399109
CSV101207207
CMO103488034 103497544
BHJ120075855
MCHA111013896
CMAX111492052
CMOS111462364
CPEP111806080
RCU8274645
JCU105639907
HBR110673830
MESC110616237 110625574
POP7490655
PEU105128853
PALZ118052715
JRE109021810
QSU111990871 112014730 112021410 112024862
QLO115974524
TWL120009391
VVI100242000
VRI117906452
SLY101259434
SPEN107015939
SOT102593085
CANN107866886
NTA107819004 107821395
NSY104231683
NTO104104526
NAU109241656
INI109152797
ITR116026784
SIND105159337
OEU111378944
EGT105958950
SSPL121754213 121755951
HAN110865884
ECAD122605183
LSV111920725 111920726 111920734
CCAV112502490
DCR108215109
CSIN114291072
BVG104897101
SOE110791440
CQI110737681
NNU104588007
MING122066589 122073320
TSS122661827
PSOM113330376 113361575
NCOL116246740 116248953
OSA4332866
DOSAOs03t0355800-01(Os03g0355800)
OBR102714357
BDI100846135
ATS109767419
TDC119301100 119308623
TAES123103313 123111503 123120725
SBI8082642
ZMA100191377
SITA101779467
SVS117837863
PVIR120652326 120652327 120688614
PHAI112874462 112874463
PDA103719882
EGU105044158
MUS103976150
DCT110104280
PEQ110033313
AOF109838810
ATR18436337 18440152
SMOSELMODRAFT_232025 SELMODRAFT_232835
PPP112277156
CRECHLRE_05g233300v5
VCNVOLCADRAFT_109194
MNGMNEG_14986
CSLCOCSUDRAFT_18056
CVRCHLNCDRAFT_54649
APROF751_4143
OLUOSTLU_40543
OTAOT_ostta16g01480
BPGBathy16g01060
MISMICPUN_88722
MPPMICPUCDRAFT_22456
CMECYME_CMJ181C
GSLGasu_02030
CCPCHC_T00008650001
SCEYLR172C(DPH5)
SEUBDI49_3689
SPAOSPAR_L02070
AGOAGOS_AFR123W
ERCEcym_2725
KLAKLLA0_F22836g
KMXKLMA_40290(DPH5)
LTHKLTH0H11748g
VPOKpol_1061p22
ZROZYRO0E05258g
CGRCAGL0B04961g
NCSNCAS_0A08800(NCAS0A08800)
NDINDAI_0G05220(NDAI0G05220)
TPFTPHA_0B03580(TPHA0B03580)
TBLTBLA_0C01150(TBLA0C01150)
TDLTDEL_0B06200(TDEL0B06200)
TGBHG536_0C02610
KAFKAFR_0I02070(KAFR0I02070)
KNGKNAG_0I01400(KNAG0I01400)
ZMKHG535_0B04490
PPAPAS_chr2-1_0577
DHADEHA2E23914g
PICPICST_78373(DPH5)
PGUPGUG_04761
SPAASPAPADRAFT_61281
LELLELG_03884
CALCAALFM_C503640WA(CaO19.6676) CAALFM_C503700CA(CaO19.6682)
CTPCTRG_05527 CTRG_05542
COTCORT_0E05000
CDUCD36_53390 CD36_53440
CTENCANTEDRAFT_97340
YLIYALI0F17138g
CLUCLUG_05937
CLUSA9F13_05g00110
CAURCJI97_001766
SLBAWJ20_4140(DPH5)
PKZC5L36_0C02340
BNNFOA43_004172(DPH5)
BBRXBRETT_001946(DPH5)
OPAHPODL_01338
NCRNCU07266
NTENEUTE1DRAFT61330(NEUTE1DRAFT_61330)
SMPSMAC_00801
PANPODANS72p127
TTTTHITE_2114762
MTMMYCTH_2298356
CTHRCTHT_0035270
MGRMGG_10496
PPEIPpBr36_00109
TMNUCRPA7_3374
SSCKSPSK_09544
FGRFGSG_09628
FPUFPSE_05908
FVRFVEG_04193
FOXFOXG_06341
NHENECHADRAFT_58379
TRETRIREDRAFT_74943
TRRM419DRAFT_5624
MAWMAC_06371
MAJMAA_05103
CMTCCM_04207
PLJVFPFJ_00834
VALVDBG_01098
VDAVDAG_00707
CFJCFIO01_03647
CHIGCH63R_13946
SAPOSAPIO_CDS8449
ELAUCREL1_9292
PFYPFICI_04920
SSLSS1G_09828
BFUBCIN_11g05290(Bcdph5)
MBEMBM_01613
PSCOLY89DRAFT_707325
GLZGLAREA_12726
ANIAN0720.2
AFMAFUA_1G14020
ACTACLA_021450
NFINFIA_011450
AORAO090012000475
ANGANI_1_1012074(An08g07220)
AFVAFLA_002196
ALUCAKAW2_30367S(DPH5)
ACHEACHE_30546A(DPH5)
APUUAPUU_11269S(DPH5)
PCSPc16g12550
PDPPDIP_67100
TMFPMAA_075010
TRGTRUGW13939_08930
CIMCIMG_03364
CPWCPC735_008450
UREUREG_07192
PBLPAAG_02178
PBNPADG_00839
ABEARB_00078
TVETRV_00926
AJEHCAG_06727
BGHBDBG_03028
PNOSNOG_03141
PTEPTT_18731
BZECOCCADRAFT_9650
BSCCOCSADRAFT_138369
BORCOCMIDRAFT_31430
AALTCC77DRAFT_996074
ZTRMYCGRDRAFT_93945
PFJMYCFIDRAFT_38626
FFUCLAFUR5_09631
BCOMBAUCODRAFT_109835
NPAUCRNP2_4698
TMLGSTUM_00008315001
SPOSPCC576.14(dph5)
CNECNF03650
CNBCNBF1170
CNGCNAG_06656
CGICGB_F1570W
TMSTREMEDRAFT_34182
TASAA1Q1_05769
PPLPOSPLDRAFT_88734
TVSTRAVEDRAFT_60574
DSQDICSQDRAFT_107082
PCOPHACADRAFT_125502
SHSSTEHIDRAFT_142522
HIRHETIRDRAFT_127120
PSQPUNSTDRAFT_121848
ADLAURDEDRAFT_113528
FMEFOMMEDRAFT_131067
GTRGLOTRDRAFT_42497 GLOTRDRAFT_8414
LBCLACBIDRAFT_334053
MRRMoror_1008
MOREE1B28_010254
CCICC1G_04639
SCMSCHCODRAFT_57471
ABPAGABI1DRAFT56726(AGABI1DRAFT_56726)
ABVAGABI2DRAFT222176(AGABI2DRAFT_222176)
CPUTCONPUDRAFT_125363
SLASERLADRAFT_456781
WSEWALSEDRAFT_60529
WICJ056_003731
UMAUMAG_04406
PFPPFL1_05725
MGLMGL_2369
MRTMRET_3286
MSYMMSY001_1711
PGRPGTG_12290
MLRMELLADRAFT_37443
ECUECU11_1430
EINEint_111320
EHEEHEL_111320
EROEROM_111320
NCENCER_101909
MBRMONBRDRAFT_33663
DDIDDB_G0276115(dph5)
DPPDICPUDRAFT_37635
DFADFA_02534(dph5)
EHIEHI_127240(129.t00009)
EDIEDI_190670
EIVEIN_379350 EIN_379610
PFAPF3D7_1009000
PFHPFHG_02658
PREIPRSY57_1008400
PGABPGSY75_1009000
PYOPY17X_1210500
PCBPCHAS_120800(PC000390.05.0)
PBEPBANKA_1207300
PVVPVVCY_1200810
PKNPKNH_0808700
PVXPVX_094665
PCYPCYB_081860
TANTA03050
TPVTP01_0634
TOTTOT_010000583
BEQBEWA_045850
BBOBBOV_IV002920(21.m02908)
BMICBMR1_03g01210
BBIGBBBOND_0111270
CPVcgd6_1200
CHOChro.60154
TGOTGME49_249920
TETTTHERM_00833850
PTMGSPATT00026998001 GSPATT00028621001
PTIPHATRDRAFT_5236
FCYFRACYDRAFT_186536
TPSTHAPSDRAFT_20788
AAFAURANDRAFT_29030
PIFPITG_11907
SPARSPRG_18083
EHXEMIHUDRAFT_236620 EMIHUDRAFT_246613
GTTGUITHDRAFT_158589 GUITHDRAFT_49003
TBRTb927.4.4650
TBGTbgDal_IV4830
TCR407477.30 511389.130
LMALMJF_31_1610
LIFLINJ_31_1640
LDOLDBPK_311640
LMILMXM_30_1610
LBZLBRM_31_1820
LPANLPMP_311540
GLAGL50803_0028666
 » show all
Reference
1  [PMID:3042777]
  Authors
Chen JY, Bodley JW.
  Title
Biosynthesis of diphthamide in Saccharomyces cerevisiae. Partial purification and characterization of a specific S-adenosylmethionine:elongation factor 2 methyltransferase.
  Journal
J Biol Chem 263:11692-6 (1988)
Reference
2  [PMID:3346227]
  Authors
Moehring JM, Moehring TJ.
  Title
The post-translational trimethylation of diphthamide studied in vitro.
  Journal
J Biol Chem 263:3840-4 (1988)
Reference
3  [PMID:24739148]
  Authors
Lin Z, Su X, Chen W, Ci B, Zhang S, Lin H
  Title
Dph7 catalyzes a previously unknown demethylation step in diphthamide biosynthesis.
  Journal
J Am Chem Soc 136:6179-82 (2014)
DOI:10.1021/ja5009272
  Sequence
[sce:YLR172C]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.314
IUBMB Enzyme Nomenclature: 2.1.1.314
ExPASy - ENZYME nomenclature database: 2.1.1.314
BRENDA, the Enzyme Database: 2.1.1.314

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