KEGG   ENZYME: 2.1.1.41
Entry
EC 2.1.1.41                 Enzyme                                 
Name
sterol 24-C-methyltransferase;
Delta24-methyltransferase;
Delta24-sterol methyltransferase;
zymosterol-24-methyltransferase;
S-adenosyl-4-methionine:sterol Delta24-methyltransferase;
SMT1;
24-sterol C-methyltransferase;
S-adenosyl-L-methionine:Delta24(23)-sterol methyltransferase;
phytosterol methyltransferase
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:zymosterol 24-C-methyltransferase
Reaction(IUBMB)
S-adenosyl-L-methionine + 5alpha-cholesta-8,24-dien-3beta-ol = S-adenosyl-L-homocysteine + 24-methylene-5alpha-cholest-8-en-3beta-ol [RN:R04427]
Reaction(KEGG)
R04427;
(other) R07481
Substrate
S-adenosyl-L-methionine [CPD:C00019];
5alpha-cholesta-8,24-dien-3beta-ol [CPD:C05437]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
24-methylene-5alpha-cholest-8-en-3beta-ol [CPD:C04525]
Comment
Requires glutathione. Acts on a range of sterols with a 24(25)-double bond in the sidechain. While zymosterol is the preferred substrate it also acts on desmosterol, 5alpha-cholesta-7,24-dien-3beta-ol, 5alpha-cholesta-5,7,24-trien-3beta-ol, 4alpha-methylzymosterol and others. S-Adenosyl-L-methionine attacks the Si-face of the 24(25) double bond and the C-24 hydrogen is transferred to C-25 on the Re face of the double bond.
History
EC 2.1.1.41 created 1972, modified 2001
Pathway
ec00100  Steroid biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K00559  sterol 24-C-methyltransferase
Genes
BMYBM_BM4647(Bma-strm-1)
NAINECAME_03546
AQU100639491
ATHAT5G13710(SMT1)
ALY9307650
CRB17882375
CSAT104705624 104735382 104769590
EUSEUTSA_v10014014mg
BRP103850891 103856028
BNA106371823 106384356 106392091 106431054 106440921
BOE106322658 106329263
RSZ108839810 108844631
THJ104809850 104819084 104819086 104819087
CPAP110809321
CIT102628258
CICCICLE_v10031748mg
PVY116132255 116132258
MINC123197557 123214703 123216668 123216669 123216670
TCC18598956 18614599 18614600
GRA105790583
GHI107915814 107950679
GAB108470188
DZI111305861 111313689
EGR104454641 104454642 104455966
GMX100796978 100817534 100818061 548073
GSJ114372208 114378245 114379538 114386323
PVUPHAVU_005G023700g PHAVU_011G011800g PHAVU_011G011900g
VRA106760797
VAR108332339
VUN114165537
CCAJ109799147 109806010
APRC113854972 113865316
MTR11415411
CAM101497864
LJALj3g3v0825030.1(Lj3g3v0825030.1) Lj3g3v0825030.2(Lj3g3v0825030.2)
ADU107471578 107479358
AIP107622756 107630475
AHF112716746 112738324 112783834 112790500
LANG109333713 109346157
FVE101293342
RCN112181939
PPER18785581
PMUM103330640
PAVI110765835
PDUL117619806
MDM103418631 103438422 114823791
ZJU107425735 107431451
MNT112091791 21388176
CSV101217151 101217389
CMO103500325 103500326
BHJ120089794 120091030
MCHA111020326 111020366
CMAX111481504 111481505 111493094
CMOS111441927 111442447 111452027
CPEP111787513 111789805 111789806
RCU8259297 8286298
JCU105628730
HBR110634256 110659984
MESC110618568 110620358 110623913
POP18095100 18106161 7467341
PEU105117376 105126337 105131854 105141731
PALZ118027720 118047027 118051651
JRE108987693 108988702 109019397
QSU112022102 112025906 112027940 112029097
QLO115953357 115967771 115986962
TWL120011980 120014669
VVI100252116 100263590
VRI117919779 117928779 117928780
SLY101252433 101256047
SPEN107008176 107032350
SOT102581721 102589132
SSTN125853521 125866069
CANN107839483 107845155
NTA107772103 107806298 107813194 107813488 107821929 107825894
NSY104213731 104221372 104248706
NTO104104807 104117345 104120777
NAU109216067 109225324 109237978
INI109156026 109156027
ITR115995625 115997067
SIND105169293
OEU111410481 111410482
EGT105969542
SSPL121759270 121791175 121805340 121809614
HAN110888576 110918161 110921577
ECAD122595873
LSV111900335 111912645 111912658
CCAV112522572
DCR108196395 108226507
CSIN114279643 114312087 114318153
BVG104907757
SOE110778345
CQI110702765 110705694
NNU104597375
MING122071445 122071905 122091901
TSS122642777 122642790 122642791
PSOM113313126 113322869 113354420
NCOL116249160
OSA4342690 9269350
DOSAOs03t0807600-00(Os03g0807600) Os07t0206700-01(Os07g0206700) Os11t0296900-00(Os11g0296900)
OBR102713236
BDI100826864 100834229
ATS109734415 109756778
TDC119291992 119304087
TAES123093722 123105904 543246
TUA125529735
SBI8057107 8064726 8078421 8081208
ZMA100282501 100286381 541682
SITA101753762 101756008 101780888
SVS117834191 117845345 117866533
PVIR120639714 120644214 120644215 120644245 120645629 120649372 120659670 120665784
PHAI112872433 112877095 112883041
PDA103696988 103696989 103697536 103717656 103717657 113461272 120104527
EGU105035517 105046511 105055167
MUS103979889 103980161 103981384 103993068
DCT110109799 110110369 110116065
PEQ110018546 110018549
AOF109833715
ATR18428920 18428921 18428922
SMOSELMODRAFT_449609(SMT1-2) SELMODRAFT_449613(SMT1-1)
PPP112291818
MNGMNEG_11110
OLUOSTLU_30710
OTAOT_ostta03g03830
BPGBathy09g04120
MISMICPUN_93596(SMT1)
MPPMICPUCDRAFT_50903
CMECYME_CMP275C
GSLGasu_26310 Gasu_60150
SCEYML008C(ERG6)
SEUBDI49_4081
SPAOSPAR_M01190
AGOAGOS_ADR196W
ERCEcym_4063
KLAKLLA0_A01738g
KMXKLMA_30671(ERG6)
LTHKLTH0G03806g
VPOKpol_1004p23
ZROZYRO0D12606g
CGRCAGL0H04653g
NCSNCAS_0H02570(NCAS0H02570)
NDINDAI_0C01050(NDAI0C01050)
TPFTPHA_0D02350(TPHA0D02350)
TBLTBLA_0D01630(TBLA0D01630)
TDLTDEL_0A03930(TDEL0A03930)
TGBHG536_0A05480
KAFKAFR_0C05410(KAFR0C05410)
KNGKNAG_0B03670(KNAG0B03670)
ZMKHG535_0D04960
PPAPAS_chr3_0371
DHADEHA2D17622g
PICPICST_80971
PGUPGUG_00661
SPAASPAPADRAFT_62031
LELLELG_02736
CALCAALFM_C302150CA(ERG6)
CTPCTRG_02396
COTCORT_0C04430
CDUCD36_82130(ERG6)
CTENCANTEDRAFT_114072
YLIYALI0F08701g
CLUCLUG_02673
CLUSA9F13_07g01804
CAURCJI97_005423
SLBAWJ20_2170(ERG6)
PKZC5L36_0E02630
BNNFOA43_002150(ERG6)
BBRXBRETT_003844(ERG6)
OPAHPODL_01877
SLUDSCDLUD_003309
NCRNCU02502 NCU03006(erg-4)
NTENEUTE1DRAFT150553(NEUTE1DRAFT_150553) NEUTE1DRAFT73978(NEUTE1DRAFT_73978)
SMPSMAC_01214 SMAC_05969
PANPODANSg3800 PODANSg4053 PODANSg4704
TTTTHITE_68084
MTMMYCTH_110670 MYCTH_42269
CTHRCTHT_0006340
MGRMGG_04346 MGG_10568 MGG_10860
PPEIPpBr36_03960 PpBr36_10036
TMNUCRPA7_4321 UCRPA7_5376 UCRPA7_8731
SSCKSPSK_06325 SPSK_06557 SPSK_06653
FGRFGSG_02783 FGSG_05740
FPUFPSE_05610 FPSE_08317
FVRFVEG_05904 FVEG_13091
FOXFOXG_08765 FOXG_15629
NHENECHADRAFT_101488 NECHADRAFT_33136 NECHADRAFT_59530
TRETRIREDRAFT_121166 TRIREDRAFT_56236
TRRM419DRAFT_108988 M419DRAFT_72858
MAWMAC_04281 MAC_08238
MAJMAA_02798 MAA_10087
CMTCCM_04684 CCM_08656
PLJVFPFJ_10371 VFPFJ_11119
VALVDBG_01914 VDBG_08910
VDAVDAG_01643 VDAG_06572
CFJCFIO01_00904 CFIO01_00910 CFIO01_12167
CLUPCLUP02_08421 CLUP02_11674 CLUP02_11681
CHIGCH63R_07675 CH63R_11163 CH63R_11195
SAPOSAPIO_CDS4855 SAPIO_CDS6557
ELAUCREL1_1004 UCREL1_1267 UCREL1_1354
PFYPFICI_00782 PFICI_01643 PFICI_06847 PFICI_10966
SSLSS1G_03467 SS1G_03686
BFUBCIN_04g01200 BCIN_08g01540
MBEMBM_02476 MBM_07594
PSCOLY89DRAFT_628125 LY89DRAFT_684462 LY89DRAFT_725473 LY89DRAFT_789176
GLZGLAREA_04347 GLAREA_05164
ANIAN1614.2 AN7146.2
AFMAFUA_4G03630 AFUA_4G09190
ACTACLA_048560 ACLA_054780
NFINFIA_029790 NFIA_106980
AORAO090011000289 AO090012000932 AO090023000624
ANGANI_1_1324144(An16g00300) ANI_1_256124(An14g01590) ANI_1_752184(An04g04210)
AFVAFLA_002655 AFLA_006968 AFLA_007687
ALUCAKAW2_40856S(ERG6) AKAW2_51426S
ACHEACHE_40345A ACHE_60580A(ERG6)
APUUAPUU_21581S APUU_22198S(ERG6_1) APUU_80754S(ERG6_2)
PCSPc12g14840 Pc21g10300
PDPPDIP_13570 PDIP_13580 PDIP_57520 PDIP_76970
TMFPMAA_006040
TRGTRUGW13939_07336
CIMCIMG_06139 CIMG_08444 CIMG_09630
CPWCPC735_061140
UREUREG_02519 UREG_04245
PBLPAAG_01575
PBNPADG_00204
ABEARB_05210 ARB_05997
TVETRV_02353 TRV_06345
AJEHCAG_07564
BGHBDBG_08700
PNOSNOG_08443
PTEPTT_11273
BZECOCCADRAFT_22623
BSCCOCSADRAFT_39890
BORCOCMIDRAFT_90360
AALTCC77DRAFT_1024280 CC77DRAFT_984927
ZTRMYCGRDRAFT_33363 MYCGRDRAFT_70113(ERG6) MYCGRDRAFT_70899 MYCGRDRAFT_98820
PFJMYCFIDRAFT_33221 MYCFIDRAFT_64115 MYCFIDRAFT_84237
FFUCLAFUR5_00943 CLAFUR5_09835
BCOMBAUCODRAFT_129875 BAUCODRAFT_37733 BAUCODRAFT_61348
NPAUCRNP2_1782 UCRNP2_3336
TMLGSTUM_00004489001
SPOSPBC16E9.05(erg6)
CNECNB03100
CNBCNBB2600
CNGCNAG_03819
CGICGB_A3260C
TMSTREMEDRAFT_44172
TASAA1Q1_06727
PPLPOSPLDRAFT_89636
TVSTRAVEDRAFT_74073
DSQDICSQDRAFT_141756
PCOPHACADRAFT_252042
SHSSTEHIDRAFT_119615
HIRHETIRDRAFT_388200
PSQPUNSTDRAFT_126433
ADLAURDEDRAFT_110716 AURDEDRAFT_121438 AURDEDRAFT_139717
FMEFOMMEDRAFT_23815
GTRGLOTRDRAFT_122852
LBCLACBIDRAFT_184995(erg6)
CCICC1G_05622 CC1G_06149 CC1G_07728
ABPAGABI1DRAFT115299(AGABI1DRAFT_115299)
ABVAGABI2DRAFT194260(AGABI2DRAFT_194260)
PCUBJR316_0008774
MRRMoror_12565
MOREE1B28_012717
SCMSCHCO_02052297(SCHCODRAFT_02052297) SCHCO_02512248(SCHCODRAFT_02512248) SCHCO_02601905(SCHCODRAFT_02601905) SCHCO_02627257(SCHCODRAFT_02627257) SCHCO_02680330(SCHCODRAFT_02680330) SCHCO_02751485(SCHCODRAFT_02751485)
CPUTCONPUDRAFT_138796
SLASERLADRAFT_457598
WSEWALSEDRAFT_15798
WICJ056_002357
UMAUMAG_03182
PFPPFL1_05601
MGLMGL_2877
MRTMRET_3473
MSYMMSY001_0359
PGRPGTG_02203 PGTG_17346
MLRMELLADRAFT_41694 MELLADRAFT_73949
MBRMONBRDRAFT_33702
DDIDDB_G0288907(smt1)
DPPDICPUDRAFT_92595
DFADFA_09943(smt1)
ACANACA1_193300 ACA1_237870
SMINv1.2.010320.t1(symbB.v1.2.010320.t1) v1.2.020967.t1(symbB.v1.2.020967.t1) v1.2.040208.t1(symbB.v1.2.040208.t1)
PTIPHATRDRAFT_10824
TPSTHAPSDRAFT_32029 THAPSDRAFT_36203(SMT1)
AAFAURANDRAFT_21939 AURANDRAFT_52760 AURANDRAFT_59110
SPARSPRG_05001
EHXEMIHUDRAFT_432780
GTTGUITHDRAFT_63072
TBRTb10.6k15.3820
TBGTbgDal_X8450
TCR504191.10 505683.10 510185.10
LMALMJF_36_2380 LMJF_36_2390(LmSMT)
LIFLINJ_36_2510 LINJ_36_2520
LDOLDBPK_362510 LDBPK_362520
LMILMXM_36_2380 LMXM_36_2390
LBZLBRM_35_2600
LPANLPMP_352450(LMSMT)
NGRNAEGRDRAFT_81379 NAEGRDRAFT_83028
TMCLMI_2904
SALOEF888_11230
SRISELR_21420
SRKFGW37_00260
NDKI601_4050(rebM_4)
AEBC6I20_03155
FRIFraEuI1c_2154
ABAIIMCC26256_112265
SUSAcid_3267
AEVEI546_13790
 » show all
Reference
1  [PMID:5354959]
  Authors
Moore JT, Gaylor JL.
  Title
Isolation and purification of an S-adenosylmethionine: delta 24-sterol methyltransferase from yeast.
  Journal
J Biol Chem 244:6334-40 (1969)
Reference
2  [PMID:8597586]
  Authors
Venkatramesh M, Guo DA, Jia Z, Nes WD.
  Title
Mechanism and structural requirements for transformation of substrates by the (S)-adenosyl-L-methionine:delta 24(25)-sterol methyl transferase from Saccharomyces cerevisiae.
  Journal
Biochim Biophys Acta 1299:313-24 (1996)
DOI:10.1016/0005-2760(95)00218-9
Reference
3
  Authors
Tong, Y., McCourt, B.S., Guo, D., Mangla, A.T., Zhou, W.X., Jenkins, M.D., Zhou, W., Lopez, M. and Nes, W.D.
  Title
, Stereochemical features of C-methylation on the path to Delta24(28)-methylene and Delta24(28)-ethylidene sterols: studies on the recombinant phytosterol methyl transferase from Arabidopsis thaliana.
  Journal
Tetrahedron Lett 38:6115-6118 (1997)
Reference
4  [PMID:9746350]
  Authors
Bouvier-Nave P, Husselstein T, Benveniste P
  Title
Two families of sterol methyltransferases are involved in the first and the second methylation steps of plant sterol biosynthesis.
  Journal
Eur J Biochem 256:88-96 (1998)
DOI:10.1046/j.1432-1327.1998.2560088.x
  Sequence
[osa:4342690]
Reference
5  [PMID:9606964]
  Authors
Nes WD, McCourt BS, Zhou WX, Ma J, Marshall JA, Peek LA, Brennan M.
  Title
Overexpression, purification, and stereochemical studies of the recombinant (S)-adenosyl-L-methionine: delta 24(25)- to delta 24(28)-sterol methyl transferase enzyme from Saccharomyces cerevisiae.
  Journal
Arch Biochem Biophys 353:297-311 (1998)
DOI:10.1006/abbi.1998.0665
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.41
IUBMB Enzyme Nomenclature: 2.1.1.41
ExPASy - ENZYME nomenclature database: 2.1.1.41
BRENDA, the Enzyme Database: 2.1.1.41
CAS: 37257-07-1

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