KEGG   ENZYME: 2.1.1.98
Entry
EC 2.1.1.98                 Enzyme                                 
Name
diphthine synthase;
S-adenosyl-L-methionine:elongation factor 2 methyltransferase (ambiguous);
diphthine methyltransferase (ambiguous);
S-adenosyl-L-methionine:2-(3-carboxy-3-aminopropyl)-L-histidine-[translation elongation factor 2] methyltransferase;
Dph5 (ambiguous)
Class
Transferases;
Transferring one-carbon groups;
Methyltransferases
Sysname
S-adenosyl-L-methionine:2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] methyltransferase (diphthine-[translation elongation factor 2]-forming)
Reaction(IUBMB)
3 S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] = 3 S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2] (overall reaction) [RN:R10306];
(1a) S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] = S-adenosyl-L-homocysteine + 2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2] [RN:R04481];
(1b) S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2] = S-adenosyl-L-homocysteine + 2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2] [RN:R08468];
(1c) S-adenosyl-L-methionine + 2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2] = S-adenosyl-L-homocysteine + diphthine-[translation elongation factor 2] [RN:R08469]
Reaction(KEGG)
Substrate
S-adenosyl-L-methionine [CPD:C00019];
2-[(3S)-3-carboxy-3-aminopropyl]-L-histidine-[translation elongation factor 2] [CPD:C04441];
2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2] [CPD:C04692];
2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2] [CPD:C16720]
Product
S-adenosyl-L-homocysteine [CPD:C00021];
diphthine-[translation elongation factor 2] [CPD:C01573];
2-[(3S)-3-carboxy-3-(methylamino)propyl]-L-histidine-[translation elongation factor 2] [CPD:C04692];
2-[(3S)-3-carboxy-3-(dimethylamino)propyl]-L-histidine-[translation elongation factor 2] [CPD:C16720]
Comment
This archaeal enzyme produces the trimethylated product diphthine, which is converted into diphthamide by EC 6.3.1.14, diphthine---ammonia ligase. Different from the eukaryotic enzyme, which produces diphthine methyl ester (cf. EC 2.1.1.314). In the archaeon Pyrococcus horikoshii the enzyme acts on His600 of elongation factor 2.
History
EC 2.1.1.98 created 1990, modified 2013, modified 2015
Orthology
K20215  diphthine synthase
Genes
MANIDP067_03580
MJAMJ_1274
MFEMefer_0044
MVUMetvu_1279
MFSMFS40622_0657
MIFMetin_0832
MJHJH146_0451
MESGMLAUSG7_0028(dphB)
MESAMLASG1_1208(dphB)
MIGMetig_1174
MMPMMP0588(dph5)
MMQMmarC5_1019
MMXMmarC6_0305
MMZMmarC7_1609
MMDGYY_03540
MMAKMMKA1_06360
MMAOMMOS7_06310
MMADMMJJ_03970
MAEMaeo_0435
MVNMevan_1459
MVOMvol_0810
MOKMetok_0033
METFCFE53_00595
MTHMTH_1874
MMGMTBMA_c04450(dphB)
METCMTCT_1712
MWOMWSIV6_0405(dphB)
METEtca_01810
METZMETMT2_0209
METKFVF72_02335(dph5)
MTHMFZP57_01900(dph5)
METJFZP68_06930(dph5)
MTEEMTTB_11810
MSTMsp_1017(dphB)
METBAW729_10810
MRUmru_1764(dphB)
MSIMsm_0801
MEBAbm4_1315(dphB)
MMILsm9_1721(dphB)
MEYETL18_07670
MOLYLM1_1219
MAROMarbSA_07220
MELMetbo_0693
MEWMSWAN_1562
METHMBMB1_1281(dphB)
MFCBRM9_0500(dphB)
MFIDSM1535_0480(dphB)
MCUBMCBB_1499(dphB)
MSUBBK009_05330
METNBK008_00875
METTCIT01_08420
METOCIT02_11640
MEMEHYG87_08875
MFVMfer_0700
MKAMK0747(dph5)
AFUAF_0381
AFGAFULGI_00003810
APOArcpr_0161
AVEArcve_1504
ASTAsulf_00669
FPLFerp_1481
GACGACE_1631
GAHGAH_00943
PFUPF0595
PFIPFC_02105
PHOPH0725(PH0725)
PABPAB1501
PYNPNA2_1369
PYAPYCH_07540
PYSPy04_0739
PYCTQ32_04245
TKOTK0106
TONTON_1372
TGATGAM_0314(dph5)
TSITSIB_0124
TBATERMP_01321
THEGQS_08695
THATAM4_1375
THMCL1_1363
TLTOCC_06971
THSTES1_1349
TNUBD01_2145
TEUTEU_05045
TGYX802_05025
THVADU37_CDS00450
TCHCHITON_1118
TPEPA0127_03225
TPIEA7C91_02410
TGGA3K92_07750
TCEA3L02_04310
TBSA3L01_07595
THHCDI07_07985
TSLA3L11_00445
TTDA3L14_09950
TPRFA3L09_00145
TRLA3L10_06995
TPAFA3L08_00780
THYA3L12_02145
TICFH039_10525
TCQTIRI35C_1840(dphB)
THEMFPV09_10635
PPACPAP_06625
MBAMbar_A2900
MBYMSBRM_1431
MBWMSBRW_1838
MBARMSBR2_0974
MBAKMSBR3_0271
MACMA_1370(dph5)
MMAMM_2355
MMAZMmTuc01_2405
MMJMSMAS_2344
MMACMSMAC_2101
MVCMSVAZ_1780
MEKMSKOL_1756
MLSMSLAZ_0954
METMMSMTP_2166
MEFMSWH1_2299
MEQMSWHS_2776
MSJMSSAC_1376
MSZMSSIH_1580
MSWMSSIT_1666
MTHRMSTHT_0849
MTHEMSTHC_2422
MHORMSHOH_2966
MFZAOB57_012250
MBUMbur_0971
MMETMCMEM_1474
MELOJ7W08_06130
MMHMmah_0645
MHAZBHR79_00140
MEVMetev_1417
MZHMzhil_0765
MPYMpsy_3166
MZIHWN40_07825
MHZMetho_1698
MTPMthe_1120
MCJMCON_1449(dphB)
MHIMhar_0531
MHUMhun_0788
MRTJKHC33_09675(dph5)
MLAMlab_0386
MEMMemar_1783
MBGBN140_0607(dphB)
MEMAMMAB1_0789(dphB)
MCHKMchiMG62_20420
MPIMpet_0734
MBNMboo_2068
MFOMetfor_0785
MPLMpal_0116
MPDMCP_0924(dphB)
MEZMtc_2082
RCIRRC519(dphB)
HALVNG_1118G(lds)
HSLOE_2613R(dph5)
HDLHALDL1_10650
HHBHhub_2319(dph5)
HAGSJT689_05820
HABOJRZ79_04285(dph5)
HNOLT974_07305(dph5)
HLULT972_06360(dph5)
HANRLJ422_04565(dph5)
HJEHacjB3_08510
HALHHTSR_1064(dph5)
HHSRHSR6_1101(dph5)
HSUHLASF_1105
HSFHLASA_1094
SALRFQU85_10295
HALREFA46_008765
HARAAArcS_0737(dph5)
HAADMW046_05470(dph5)
HALXM0R89_11905(dph5)
HMArrnAC1406(dph5)
HHIHAH_1989(dph5)
HHNHISP_10135
HABSG26_02425
HTABVU17_09690
HALJG9465_14495
HSINKDQ40_03505
NPHNP_1884A(dph5)
NMONmlp_1959(dph5)
NHOHWV23_04205
NSALHWV07_03245
HUTHuta_0243
HTIHTIA_0426
HALAHrd1104_10395
HMUHmuk_1953
HALZE5139_12875
HALLLC1Hm_1275(dph5)
HAZPGBQ70_12890
HALIBV210_14315
HSNDV733_02505
HDSHSR122_2165(dph5)
HRRHZS55_11000
HPELHZS54_15390
HLTI7X12_03015
HARCHARCEL1_11525(dph5)
HDOMUK72_06720(dph5)
HWAHQ_1603A(dph5)
HWCHqrw_1714(dph5)
HVOHVO_0916(dph5)
HMEHFX_0887(dph5)
HGIABY42_04405
HALEG3A49_04580
HBOHbor_23080
HAQDU484_14240
HAJDU500_14270
HAERDU502_12385
HRAEI982_17120
HLMDV707_10280
HALMFCF25_11605
HLAHlac_0892
HALPDOS48_03675
HALBEKH57_07480
HEZZEO776_04900
HALQHrr1229_002320
HSSJ7656_11935
HSAIHPS36_02260
HAZZKI388_02455(dph5)
SRUBC2R22_05630
HAEhalTADL_1046
HALNB4589_000415
HALGHUG10_14700
HALUHUG12_01595
HMPK6T50_10855(dph5)
HREK6T36_10640(dph5)
HRMK6T25_05420(dph5)
HACBHbl1158_02650(dph5)
HDFAArcSl_0117(dph5)
HAHHalar_0827
HTUHtur_2752
HDABB347_09440
HJTDVR14_14165
HSALJMJ58_14720
HLOJ0X27_11790
HAKZJ0X25_01980(dph5)
NMGNmag_0897(dph5)
HXAHalxa_2402
NATNJ7G_1756
NPENatpe_0971
NVRFEJ81_05005
NPLFGF80_05800
NAYHYG81_06255(dph5)
HALYHYG82_08855
NGENatgr_0089
HRUHalru_1978
NOUNatoc_2717
SALIL593_14760
SAIMK0C01_02330(dph5)
HLRHALLA_08355
HLCCHINAEXTREME08855
NAJB1756_01955
NAGAArcMg_0796
NANAArc1_2709
NBGDV706_10350
NASGCU68_01725
NACAQV86_05655
MELUMTLP_09690
MEAMMU439_07250(dph5)
TACTa0883
TVOTVG1034064(TVG1034064)
PTOPTO1233
FACFACI_IFERC01G1335
FAIFAD_0348
CDIVCPM_1291
TARTALC_01129
MAXMMALV_11700
MERMMINT_14280
MEARMpt1_c02170(dphB)
MARCAR505_1284
ABIAboo_0591
ACFAciM339_0455
APEAPE_0931(dphB)
ACJACAM_0608(dphB)
SMRSmar_0509
SHCShell_0288
IHOIgni_0657
IISEYM_06210
DKADKAM_1445
DFDDesfe_0069
DMUDesmu_0139
TAGTagg_1079
IAGIgag_0048
THGTCELL_0750
HBUHbut_0128
PFMPyrfu_1089
PDLPyrde_0677
STOSTK_12780(dphB)
SOHD1869_07400
SSOSSO0953
SOLSsol_1929
SSOASULA_1961
SSOLSULB_1962
SSOFSULC_1960
SSHIJ5U23_02525
SCASSACC_08370
SAISaci_1334
SACNSacN8_06505
SACRSacRon12I_06495
SACSSUSAZ_06375
SISLS215_1360
SIAM1425_1273
SIMM1627_1323
SIDM164_1257
SIYYG5714_1258
SINYN1551_1590
SIILD85_1385
SIHSiH_1218
SIRSiRe_1136
SICSiL_1133
SULABFU36_01670
SULEGFS03_07145
SULLEWF20_08575
MSEMsed_1735
MCNMcup_0493
MHKDFR87_09985
MPRUDFR88_09560
MTENGWK48_02290
MJNMjAS7_2863
AHOAhos_1400
AMANB6F84_11460
ABRIDFR85_10815
ASULDFR86_09775
AAMBD1866_01935
ACIHHS5_28120
SACDHS1genome_1023
SAZOD1868_04320
CSTYKN1_24530
STEPIC006_1161
SAHSHS7_18650
PAIPAE1139
PISPisl_1283
PCLPcal_0705
PASPars_0230
PYRP186_2537
POGPogu_2233
TNETneu_0216
PYWPYWP30_00283
CMACmaq_1990
TTNTTX_0095(dph5)
TUZTUZN_1249
VDIVdis_2028
VMOVMUT_0435
TPETpen_0689
THBN186_04685
TCBTCARB_1964
THELIG193_03945(dph5)
ASCASAC_0414
CLGCalag_1209
FFOFFONT_1021
NMRNmar_1297
NIRNSED_07195
NIDNPIRD3C_0827
NINNADRNF5_0659
NIWNisw_07245(dph5)
NCLC5F47_06985(dph5)
NOXC5F49_06995(dph5)
NUEC5F50_09750(dph5)
NIPNsoK4_01555(dph5)
NCTNMSP_0391(dphB)
NICDSQ20_07950(dph5)
CSYCENSYa_0126
NGANgar_c14540
NVNNVIE_002640
NEVNTE_01580
TAANMY3_02230
NFNNFRAN_2992
NCVNCAV_1393
CSUCSUB_C1500
NBVT478_1052(dph5)
TAHSU86_009035
NIUDSQ19_03180(dph5)
NDVNDEV_1632(dph)
CCAINAS2_0256
BARCAOA65_2396(dphB)
BARBAOA66_1245(dphB)
NEQNEQ422
NAANps_01115
MARHMia14_0002
FLTSv326_0989
MIYMicr_00043(dphB_2)
NCONLC1Nh_0925(dph5)
AGWQT03_C0001G0336
ARGQT11_C0001G0598
 » show all
Reference
1  [PMID:20873788]
  Authors
Zhu X, Kim J, Su X, Lin H
  Title
Reconstitution of diphthine synthase activity in vitro.
  Journal
Biochemistry 49:9649-57 (2010)
DOI:10.1021/bi100812h
  Sequence
[pho:PH0725]
Other DBs
ExplorEnz - The Enzyme Database: 2.1.1.98
IUBMB Enzyme Nomenclature: 2.1.1.98
ExPASy - ENZYME nomenclature database: 2.1.1.98
BRENDA, the Enzyme Database: 2.1.1.98
CAS: 114514-25-9

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