KEGG   ENZYME: 2.1.3.10
Entry
EC 2.1.3.10                 Enzyme                                 
Name
malonyl-S-ACP:biotin-protein carboxyltransferase;
malonyl-S-acyl-carrier protein:biotin-protein carboxyltransferase;
MadC/MadD;
MadC,D;
malonyl-[acyl-carrier protein]:biotinyl-[protein] carboxyltransferase
Class
Transferases;
Transferring one-carbon groups;
Carboxy- and carbamoyltransferases
Sysname
malonyl-[acyl-carrier protein]:biotinyl-[protein] carboxytransferase
Reaction(IUBMB)
a malonyl-[acyl-carrier protein] + a biotinyl-[protein] = an acetyl-[acyl-carrier protein] + a carboxybiotinyl-[protein] [RN:R08936]
Reaction(KEGG)
R08936
Substrate
malonyl-[acyl-carrier protein] [CPD:C01209];
biotinyl-[protein] [CPD:C18046]
Product
acetyl-[acyl-carrier protein] [CPD:C03939];
carboxybiotinyl-[protein] [CPD:C18047]
Comment
Derived from the components MadC and MadD of the anaerobic bacterium Malonomonas rubra, this enzyme is a component of EC 7.2.4.4, biotin-dependent malonate decarboxylase. The carboxy group is transferred from malonate to the prosthetic group of the biotin protein (MadF) with retention of configuration [2]. Similar to EC 4.1.1.87, malonyl-S-ACP decarboxylase, which forms part of the biotin-independent malonate decarboxylase (EC 4.1.1.88), this enzyme also follows on from EC 2.3.1.187, acetyl-S-ACP:malonate ACP transferase, and results in the regeneration of the acetyl-[acyl-carrier protein] [3].
History
EC 2.1.3.10 created 2008, modified 2018
Orthology
K20510  malonyl-S-ACP:biotin-protein carboxyltransferase subunit MadC
K20511  malonyl-S-ACP:biotin-protein carboxyltransferase subunit MadD
Genes
EFEEFER_3130 EFER_3131
KPZKPNIH27_22440 KPNIH27_22445
KPBFH42_14170 FH42_14175
KLWDA718_03335(mdcE) DA718_03340
KGRJJJ10_02970(mdcE) JJJ10_02975
CAMAF384_01155 F384_01160
CTELGBC03_24695 GBC03_24700(mdcE)
SFOZ042_24845 Z042_26175
DESDSOUD_0385 DSOUD_0386
RPBRPB_3295 RPB_3296
RPCRPC_1059 RPC_1060
RCPRCAP_rcp00127(mdcE) RCAP_rcp00128(mdcB)
RAPRHOA_4940(madC) RHOA_4941(madD)
ATIAL072_31130 AL072_31135
AZZDEW08_20130(mdcE) DEW08_20135
RRURru_A1324 Rru_A1325
RRFF11_06850 F11_06855
PFTJBW_01392 JBW_01393
 » show all
Reference
1  [PMID:9128730]
  Authors
Berg M, Hilbi H, Dimroth P
  Title
Sequence of a gene cluster from Malonomonas rubra encoding components of the malonate decarboxylase Na+ pump and evidence for their function.
  Journal
Eur J Biochem 245:103-15 (1997)
DOI:10.1111/j.1432-1033.1997.00103.x
  Sequence
Reference
2
  Authors
Micklefield, J., Harris, K.J., Groger, S., Mocek, U., Hilbi, H., Dimroth, P. and Floss, H.G.
  Title
Stereochemical course of malonate decarboxylase in Malonomonas rubra has biotin decarboxylation with retention.
  Journal
J Am Chem Soc 117:1153-1154 (1995)
Reference
3  [PMID:11902724]
  Authors
Dimroth P, Hilbi H
  Title
Enzymic and genetic basis for bacterial growth on malonate.
  Journal
Mol Microbiol 25:3-10 (1997)
DOI:10.1046/j.1365-2958.1997.4611824.x
Other DBs
ExplorEnz - The Enzyme Database: 2.1.3.10
IUBMB Enzyme Nomenclature: 2.1.3.10
ExPASy - ENZYME nomenclature database: 2.1.3.10
BRENDA, the Enzyme Database: 2.1.3.10

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