KEGG   ENZYME: 2.3.1.252
Entry
EC 2.3.1.252                Enzyme                                 
Name
mycolipanoate synthase;
msl3 (gene name);
Pks3/4;
mycolipanoic acid synthase;
long-chain acyl-CoA:methylmalonyl-CoA C-acyltransferase (mycolipanoate-forming)
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
long-chain acyl-CoA:(S)-methylmalonyl-CoA C-acyltransferase (mycolipanoate-forming)
Reaction(IUBMB)
a long-chain acyl-CoA + 3 (S)-methylmalonyl-CoA + 6 NADPH + 6 H+ + holo-[mycolipanoate synthase] = mycolipanoyl-[mycolipanoate synthase] + 4 CoA + 3 CO2 + 6 NADP+ + 3 H2O
Reaction(KEGG)
(other) R05189 R11451
Substrate
long-chain acyl-CoA [CPD:C02843];
(S)-methylmalonyl-CoA [CPD:C00683];
NADPH [CPD:C00005];
H+ [CPD:C00080];
holo-[mycolipanoate synthase]
Product
mycolipanoyl-[mycolipanoate synthase];
CoA [CPD:C00010];
CO2 [CPD:C00011];
NADP+ [CPD:C00006];
H2O [CPD:C00001]
Comment
This mycobacterial enzyme accepts long-chain fatty acyl groups from their CoA esters and extends them by incorporation of three methylmalonyl (but not malonyl) residues, forming trimethyl-branched fatty-acids such as (2S,4S,6S)-2,4,6-trimethyltetracosanoate (C27-mycolipanoate). Since the enzyme lacks a thioesterase domain, the product remains bound to the enzyme and requires additional enzyme(s) for removal.
History
EC 2.3.1.252 created 2016, modified 2019
Orthology
K12432  mycolipanoate synthase
Genes
MTURv1180(pks3) Rv1181(pks4)
MTVRVBD_1181
MTCMT1218(mas-1)
MRAMRA_1191(pks3)
MTFTBFG_11205
MTBTBMG_02801
MTKTBSG_02815
MTZTBXG_002781
MTECCDC5079_1092
MTURCFBS_1259(pks3)
MTLCCDC5180_1085
MTOMTCTRI2_1212
MTDUDA_1180(pks3) UDA_1181(pks4)
MTNERDMAN_1323(pks4)
MTJJ112_06380
MTUBMT7199_1210(pks3_4)
MTUCJ113_08285
MTUEJ114_06380
MTXM943_06185
MTULTBHG_01164
MTUTHKBT1_1257(pks3)
MTUUHKBT2_1263(pks3)
MTQHKBS1_1261(pks3)
MBOBQ2027_MB1213(pks3)
MBBBCG_1243(pks3)
MBTJTY_1216(pks3)
MBMBCGMEX_1215(pks3)
MBKK60_012730
MBXBCGT_1012
MAFMAF_11990(pks3) MAF_12000(pks4)
MMICRN08_1325
MCEMCAN_11911(pks3) MCAN_11921(pks4)
MCQBN44_11320(pks)
MCVBN43_30248(pks)
MCXBN42_21046(pks)
MCZBN45_30243(pks)
MLEML1229(pks3)
MLBMLBr01229(pks3)
MPAMAP_2603c MAP_2604c
MAOMAP4_1214 MAP4_1215
MAVMAV_1321
MITOCO_12290
MIAOCU_12250
MIDMIP_01963
MYOOEM_12440
MCHIAN480_07195
MIROCQ_12310
MMALCKJ54_05970
MLPMLM_1249
MMANMMAN_27710(pks3)
MMMW7S_06000
MHADB586_07465
MSHGMSG_03827(pks3)
MSAKMSAS_21100(pks3)
MGROFZ046_08050
MSEOMSEO_26040(pks3)
MLMMLPF_1673
MVMMJO54_23320
MCHTMCHIJ_40910
MMAGMMAD_05460(msl3)
MMATMMAGJ_61720(msl3)
MBOKMBOE_44850(pks3)
MMINMMIN_17140(pks3)
MHIBMHIB_35870(pks3)
 » show all
Reference
1  [PMID:11278910]
  Authors
Sirakova TD, Thirumala AK, Dubey VS, Sprecher H, Kolattukudy PE
  Title
The Mycobacterium tuberculosis pks2 gene encodes the synthase for the hepta- and octamethyl-branched fatty acids required for sulfolipid synthesis.
  Journal
J Biol Chem 276:16833-9 (2001)
DOI:10.1074/jbc.M011468200
Reference
2  [PMID:12207710]
  Authors
Dubey VS, Sirakova TD, Kolattukudy PE
  Title
Disruption of msl3 abolishes the synthesis of mycolipanoic and mycolipenic acids required for polyacyltrehalose synthesis in Mycobacterium tuberculosis H37Rv and causes cell aggregation.
  Journal
Mol Microbiol 45:1451-9 (2002)
DOI:10.1046/j.1365-2958.2002.03119.x
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.252
IUBMB Enzyme Nomenclature: 2.3.1.252
ExPASy - ENZYME nomenclature database: 2.3.1.252
BRENDA, the Enzyme Database: 2.3.1.252

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