KEGG   ENZYME: 2.3.1.301
Entry
EC 2.3.1.301                Enzyme                                 
Name
mycobacterial beta-ketoacyl-[acyl carrier protein] synthase III;
fabH (gene name) (ambiguous);
mycobacterial 3-oxoacyl-[acyl carrier protein] synthase III
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
dodecanoyl-CoA:malonyl-[acyl-carrier protein] C-acyltransferase
Reaction(IUBMB)
dodecanoyl-CoA + a malonyl-[acyl-carrier protein] = a 3-oxotetradecanoyl-[acyl-carrier protein] + CoA + CO2 [RN:R12735]
Reaction(KEGG)
R12735
Substrate
dodecanoyl-CoA [CPD:C01832];
malonyl-[acyl-carrier protein] [CPD:C01209]
Product
3-oxotetradecanoyl-[acyl-carrier protein] [CPD:C05759];
CoA [CPD:C00010];
CO2 [CPD:C00011]
Comment
The enzyme, characterized from mycobacteria, provides a link between the type I and type II fatty acid synthase systems (FAS-I and FAS-II, respectively) found in these organisms. The enzyme acts on medium- and long-chain acyl-CoAs (C12-C16) produced by the FAS-I system, condensing them with malonyl-[acyl-carrier protein] (malonyl-AcpM) and forming starter molecules for the FAS-II system, which elongates them into meromycolic acids. The enzyme has no activity with short-chain acyl-CoAs (e.g. acetyl-CoA), which are used by EC 2.3.1.180, beta-ketoacyl-[acyl-carrier-protein] synthase III, or branched-chain acyl-CoAs, which are used by EC 2.3.1.300, branched-chain beta-ketoacyl-[acyl-carrier-protein] synthase.
History
EC 2.3.1.301 created 2021
Orthology
K11608  mycobacterial beta-ketoacyl-[acyl-carrier-protein] synthase III
Genes
BQBJ4P68_0030340
MTURv0533c(fabH)
MTVRVBD_0533c
MTCMT0557(fabH)
MRAMRA_0540(fabH)
MTFTBFG_10544
MTBTBMG_00539
MTKTBSG_00545
MTZTBXG_000536
MTGMRGA327_03370
MTIMRGA423_03345
MTURCFBS_0557(fabH)
MTOMTCTRI2_0541(fabH)
MTDUDA_0533c(fabH)
MTJJ112_02855
MTUBMT7199_0547
MTUCJ113_03805
MTUEJ114_02855
MTXM943_02765
MTUHI917_03830
MTULTBHG_00531
MTUTHKBT1_0557(fabH)
MTUUHKBT2_0557(fabH)
MTQHKBS1_0557(fabH)
MBOBQ2027_MB0547C(fabH)
MBBBCG_0577c(fabH)
MBTJTY_0547(fabH)
MBMBCGMEX_0548c(fabH)
MBKK60_005660
MBXBCGT_0317
MAFMAF_05400(fabH)
MMICRN08_0596
MCEMCAN_05361(fabH)
MCQBN44_10593(fabH)
MCVBN43_10584(fabH)
MCXBN42_20271(fabH)
MCZBN45_10598(fabH)
MPAMAP_4028c(fabH)
MAOMAP4_4151
MAVIRC58_20595
MAVURE97_20645
MAVMAV_4612
MITOCO_44980
MIAOCU_44750
MIDMIP_06835
MYOOEM_45190
MCHIAN480_23535
MIROCQ_46100
MMALCKJ54_21430
MLPMLM_3777
MMANMMAN_33270(fabH_2)
MULMUL_0632(fabH)
MMIMMAR_0879(fabH)
MMAEMMARE11_08290(fabH)
MMMW7S_22645
MLIMULP_00907(fabH)
MKNMKAN_18700
MSHGMSG_00757(fabH)
MFJMFLOJ_27180(fabH)
MSTOMSTO_31080(fabH)
MSIMMSIM_17160(fabH)
MKUI2456_04475
MPAGC0J29_04745
MGORH0P51_04385
MBAIMB901379_00748(fabH)
MSEOMSEO_46770(fabH)
MPSEMPSD_10100(fabH)
MSHOMSHO_26380(fabH)
MLJMLAC_36200(fabH)
MBRDMBRA_09820(fabH)
MSHJMSHI_29050(fabH)
MKRMKOR_23540(fabH)
MHERK3U94_02295
MSPGF6B93_03570(fabH)
MOTLTS72_21600
MVMMJO54_02050
MFXMFAL_31900(fabH)
MIJMINS_11360(fabH)
MAUBMAUB_45000(fabH)
MPHUMPHO_24330(fabH)
MMUCC1S78_006165
MABMAB_1141c
MMVMYCMA_0605
MABBMASS_1137
MABLMMASJCM_1150
MIZBAB75_06060
MSTEMSTE_01096(fabH)
MSAOMYCSP_04930
MSALDSM43276_00997(fabH_1)
MMINMMIN_21150(fabH)
MHIBMHIB_39220(fabH)
ASDAS9A_2905(fabH) AS9A_2908(fabH3)
MPAKMIU77_15455
NFANFA_52820(fabH) NFA_53970(fabH3)
NFRERS450000_04471(fabH_4) ERS450000_04599(fabH_5) ERS450000_04925(fabH_6)
NCYNOCYR_1634(fabH) NOCYR_5108(fabH) NOCYR_5244(fabH)
NBRO3I_037595 O3I_039605 O3I_040210
NNONONO_c71510(fabH1) NONO_c72620(fabH2)
NSLBOX37_05545 BOX37_30280 BOX37_30855
NSRNS506_00291 NS506_01121 NS506_05990
NTPCRH09_37520 CRH09_38000
NOZDMB37_18410 DMB37_19015
NODFOH10_19325 FOH10_33185 FOH10_33695
NAHF5544_42075(fabH) F5544_42770(fabH)
NADNCTC11293_00255(fabH_1) NCTC11293_00382(fabH_2) NCTC11293_02068(fabH_4)
NWLNWFMUON74_49000(fabH1) NWFMUON74_67100(fabH_2) NWFMUON74_68100(fabH_3)
NIEKV110_36795 KV110_38765 KV110_39345
NTCKHQ06_03950 KHQ06_04480 KHQ06_30995
NHUH0264_10545 H0264_37245 H0264_37850
NYALTV02_04610 LTV02_33155 LTV02_33715
NGPLTT66_09560 LTT66_10155
RHARHA1_ro05206
RERRER_11360(fabH)
REYO5Y_05155
REBXU06_05400
RQIC1M55_05605
ROPROP_52560(fabH)
ROAPd630_LPD01731
RPYY013_11460
RBYCEJ39_22365
RHBNY08_3715
RAVAAT18_22395
RFAA3L23_01861(fabH)
RHSA3Q41_01504(fabH)
RHWBFN03_00840
RRZCS378_12215
RHUA3Q40_02272(fabH)
RHQIM25_03165
RHODAOT96_16005
RRT4535765_04192(fabH_2)
RCRNCTC10994_00252(fabB_1) NCTC10994_03167(fabH)
RTMG4H71_06280
RKOJWS14_28525 JWS14_39915
RGOKYT97_13280
ROZCBI38_25285
REQREQ_28490(fabB) REQ_40910(fabH)
GBRGbro_1530 Gbro_1536
GPOGPOL_c13590(fabH1) GPOL_c13640(fabH2)
GORKTR9_1451 KTR9_1454
GOQACH46_04630 ACH46_04660
GTABCM27_08040 BCM27_08055
GOCCXX93_17340 CXX93_17380
GITC6V83_05515 C6V83_05535
GRUGCWB2_07615(fabH2)
GOMD7316_01743(fabH_2)
GAVC5O27_20195
GODGKZ92_07235(fabH)
GAMGII34_06710(fabH) GII34_10700(fabH)
GPDGII33_06160(fabH) GII33_10350(fabH)
GJIH1R19_07445 H1R19_07460
GOILK459_03245 LK459_03250
GHNMVF96_07450 MVF96_07470
GAMIIHQ52_10380
SPINKV203_02315 KV203_02375
TPRTpau_2193
TSMASU32_11935
TPULTPB0596_23890(fabH1)
DTMBJL86_3010
DITC3V38_03975
DIZCT688_14605
DPCA6048_15280
DLUA6035_14580
DKNNHB83_14780
TOYFO059_01830
LMOIVV02_03980
NSNEXE58_08650
NOQLN652_12270
AMYYYIM_23715(fabH2)
 » show all
Reference
1  [PMID:11278743]
  Authors
Scarsdale JN, Kazanina G, He X, Reynolds KA, Wright HT.
  Title
Crystal structure of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthase III.
  Journal
J Biol Chem 276:20516-22 (2001)
DOI:10.1074/jbc.M010762200
  Sequence
[mtu:Rv0533c]
Reference
2  [PMID:15713483]
  Authors
Musayev F, Sachdeva S, Scarsdale JN, Reynolds KA, Wright HT.
  Title
Crystal structure of a substrate complex of Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthase III (FabH) with lauroyl-coenzyme A.
  Journal
J Mol Biol 346:1313-21 (2005)
DOI:10.1016/j.jmb.2004.12.044
  Sequence
[mtu:Rv0533c]
Reference
3  [PMID:16040614]
  Authors
Brown AK, Sridharan S, Kremer L, Lindenberg S, Dover LG, Sacchettini JC, Besra GS.
  Title
Probing the mechanism of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthase III mtFabH: factors influencing catalysis and substrate specificity.
  Journal
J Biol Chem 280:32539-47 (2005)
DOI:10.1074/jbc.M413216200
  Sequence
[mtu:Rv0533c]
Reference
4  [PMID:18420147]
  Authors
Sachdeva S, Musayev FN, Alhamadsheh MM, Scarsdale JN, Wright HT, Reynolds KA.
  Title
Separate entrance and exit portals for ligand traffic in Mycobacterium tuberculosis FabH.
  Journal
Chem Biol 15:402-12 (2008)
DOI:10.1016/j.chembiol.2008.03.007
  Sequence
[mtu:Rv0533c]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.301
IUBMB Enzyme Nomenclature: 2.3.1.301
ExPASy - ENZYME nomenclature database: 2.3.1.301
BRENDA, the Enzyme Database: 2.3.1.301

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