KEGG   ENZYME: 2.3.1.65
Entry
EC 2.3.1.65                 Enzyme                                 

Name
bile acid-CoA:amino acid N-acyltransferase;
glycine---taurine N-acyltransferase;
amino acid N-choloyltransferase;
BAT;
glycine N-choloyltransferase;
BACAT;
cholyl-CoA glycine-taurine N-acyltransferase;
cholyl-CoA:taurine N-acyltransferase
Class
Transferases;
Acyltransferases;
Transferring groups other than aminoacyl groups
Sysname
choloyl-CoA:glycine N-choloyltransferase
Reaction(IUBMB)
choloyl-CoA + glycine = CoA + glycocholate [RN:R03718]
Reaction(KEGG)
R03718;
(other) R03720 R08744 R08745
Substrate
choloyl-CoA [CPD:C01794];
glycine [CPD:C00037]
Product
CoA [CPD:C00010];
glycocholate [CPD:C01921]
Comment
Also acts on CoA derivatives of other bile acids. Taurine and 2-fluoro-beta-alanine can act as substrates, but more slowly [4]. The enzyme can also conjugate fatty acids to glycine and can act as a very-long-chain acyl-CoA thioesterase [7]. Bile-acid---amino-acid conjugates serve as detergents in the gastrointestinal tract, solubilizing long chain fatty acids, mono- and diglycerides, fat-soluble vitamins and cholesterol [4]. This is the second enzyme in a two-step process leading to the conjugation of bile acids with amino acids; the first step is the conversion of bile acids into their acyl-CoA thioesters, which is catalysed by EC 6.2.1.7, cholate---CoA ligase.
History
EC 2.3.1.65 created 1983, modified 2005
Pathway
ec00120  Primary bile acid biosynthesis
ec00430  Taurine and hypotaurine metabolism
ec01100  Metabolic pathways
Orthology
K00659  bile acid-CoA:amino acid N-acyltransferase
Genes
HSA: 570(BAAT)
PTR: 472997
PPS: 100972861 100992429(BAAT)
GGO: 101140183(BAAT)
PON: 100431907(BAAT) 100461881
NLE: 100598200(BAAT)
MCC: 100429419(BAAT)
MCF: 102132073(BAAT)
CSAB: 103219118(BAAT)
CATY: 105593023(BAAT)
PANU: 101007875(BAAT)
RRO: 104670797(BAAT)
RBB: 108537176(BAAT)
TFN: 117098906(BAAT)
PTEH: 111536312(BAAT)
CJC: 100394190 100396712(BAAT)
SBQ: 101036806
MMUR: 105873759(BAAT) 105873760
MMU: 12012(Baat) 209186(Acnat2) 230161(Acnat1)
MPAH: 110323775(Baat) 110323900
RNO: 29725(Baat) 313220(Acnat2) 681043(Acnat1)
MCOC: 116095310(Baat) 116096003
MUN: 110557337 110557338(Baat)
CGE: 100756252(Baat) 100756541
PLEU: 114702315(Baat) 114702326
HGL: 101716142(Baat) 101717293
CCAN: 109696243(Baat)
OPI: 101519913(BAAT)
TUP: 102472797 102473647(BAAT)
CFA: 481635(BAAT)
VVP: 112930766(BAAT) 112930863
UAH: 113260385 113260386(BAAT)
MPUF: 101671389 101671902(BAAT)
MLX: 118018214(BAAT) 118018215
FCA: 101095691(BAAT) 101096116
PYU: 121034756(BAAT) 121034758
PBG: 122474312 122474485(BAAT)
PTG: 102969591(BAAT) 102969870
HHV: 120224623(BAAT) 120224624
BTA: 530653 786798(BAAT)
BOM: 102268462
BIU: 109563300(BAAT)
CHX: 102174570(BAAT)
ODA: 120863959
CCAD: 122431993
CFR: 102523415(BAAT)
CBAI: 105067405(BAAT)
CDK: 105086911(BAAT)
BACU: 103006095 103017253(BAAT)
LVE: 103081315 103082496(BAAT)
OOR: 101284164(BAAT) 101284403
DLE: 111188015(BAAT)
PCAD: 102984994(BAAT)
ECB: 100054567 100063137(BAAT)
EPZ: 103552469(BAAT) 103568036
MNA: 107546604(BAAT)
HAI: 109395040(BAAT)
DRO: 112304453(BAAT)
SHON: 118979701(BAAT)
AJM: 119045945(BAAT)
MMF: 118629687(BAAT)
PALE: 102892341(BAAT) 102897591
PGIG: 120597018
MJV: 108397381(BAAT)
TOD: 119232402(BAAT)
TMU: 101357199
MDO: 100023136(BAAT)
PCW: 110219134(BAAT)
OAA: 100084056(BAAT)
GGA: 769879(BAAT)
PCOC: 116242919
MGP: 100539658
CJO: 107314177
NMEL: 110400838
APLA: 101797844
ACYG: 106048282
TGU: 115495423
LSR: 110485078
SCAN: 103822142
PMOA: 120499625
OTC: 121339708
PRUF: 121355202
GFR: 102037789
FAB: 101815920
PHI: 102101793
PMAJ: 107206148
CCAE: 111930612
ETL: 114063343
FPG: 101924136
FCH: 102059453
CLV: 102094683
EGZ: 104134405(BAAT)
NNI: 104008979(BAAT)
ACUN: 113481099
AAM: 106494986
AROW: 112960829(BAAT)
NPD: 112952722
DNE: 112988642
CMY: 102939533(BAAT) 102947935
CPIC: 101932563 101938826(BAAT)
TST: 117876003 117879669(BAAT)
CABI: 116825950(BAAT)
ACS: 100566890(baat)
PVT: 110078385(BAAT)
PBI: 103065567(BAAT)
TSR: 106538123(BAAT)
PGUT: 117667061(BAAT)
PMUA: 114600844(BAAT)
ZVI: 118089420(BAAT)
GJA: 107106123 107106126(BAAT)
XLA: 100137716(XB5872576.L) 108707337(baat.S)
SRX: 107726829
CCAR: 109068937
IPU: 108280978
EEE: 113584003
CGOB: 115008157
SLUC: 116066678
ECRA: 117944007
PFLV: 114553794
GAT: 120835251
PPUG: 119193948
CUD: 121506802
ONL: 109198392
OAU: 116329896
OLA: 101166298
OML: 112145720
XMA: 102226402
XCO: 114135870
XHE: 116711136
PRET: 103465285
CTUL: 119791826
NFU: 107385610
SDU: 111221490
SLAL: 111667779
HCQ: 109522368
MALB: 109956248
ELS: 105010081
SFM: 108933858
AANG: 118211819
HAME: 121871783
HAZT: 108682704
MYI: 110465077
OBI: 106881808
LAK: 106169569
NVE: 5519541
ADF: 107346883
AMIL: 114966874
 » show all
Reference
1  [PMID:7372637]
  Authors
Czuba B, Vessey DA.
  Title
Kinetic characterization of cholyl-CoA glycine-taurine N-acyltransferase from bovine liver.
  Journal
J Biol Chem 255:5296-9 (1980)
Reference
2
  Authors
Jordan, T.W., Lee, R. and Lim, W.C.
  Title
Isoelectric focussing of soluble and particulate benzoyl-CoA and cholyl-CoA:amino acid N-acyltransferases from rat liver.
  Journal
Biochem Int 1:325-330 (1980)
Reference
3  [PMID:422567]
  Authors
Vessey DA.
  Title
The co-purification and common identity of cholyl CoA:glycine- and cholyl CoA:taurine-N-acyltransferase activities from bovine liver.
  Journal
J Biol Chem 254:2059-63 (1979)
Reference
4  [PMID:2037576]
  Authors
Johnson MR, Barnes S, Kwakye JB, Diasio RB.
  Title
Purification and characterization of bile acid-CoA:amino acid N-acyltransferase from human liver.
  Journal
J Biol Chem 266:10227-33 (1991)
  Sequence
[hsa:570]
Reference
5  [PMID:12454267]
  Authors
Falany CN, Xie X, Wheeler JB, Wang J, Smith M, He D, Barnes S.
  Title
Molecular cloning and expression of rat liver bile acid CoA ligase.
  Journal
J Lipid Res 43:2062-71 (2002)
DOI:10.1194/jlr.m200260-jlr200
Reference
6  [PMID:12951368]
  Authors
He D, Barnes S, Falany CN.
  Title
Rat liver bile acid CoA:amino acid N-acyltransferase: expression, characterization, and peroxisomal localization.
  Journal
J Lipid Res 44:2242-9 (2003)
DOI:10.1194/jlr.M300128-JLR200
  Sequence
[rno:29725]
Reference
7  [PMID:12810727]
  Authors
O'Byrne J, Hunt MC, Rai DK, Saeki M, Alexson SE.
  Title
The human bile acid-CoA:amino acid N-acyltransferase functions in the conjugation of fatty acids to glycine.
  Journal
J Biol Chem 278:34237-44 (2003)
DOI:10.1074/jbc.M300987200
  Sequence
[hsa:570]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.1.65
IUBMB Enzyme Nomenclature: 2.3.1.65
ExPASy - ENZYME nomenclature database: 2.3.1.65
BRENDA, the Enzyme Database: 2.3.1.65
CAS: 65979-40-0

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