KEGG   ENZYME: 2.3.2.34
Entry
EC 2.3.2.34                 Enzyme                                 

Name
E2 NEDD8-conjugating enzyme;
NEDD8-carrier-protein E2;
NEDD8-conjugating enzyme E2;
UBE2M (gene name);
UBE2F (gene name)
Class
Transferases;
Acyltransferases;
Aminoacyltransferases
Sysname
[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine:[E2 NEDD8-conjugating enzyme] [NEDD8-protein]-yl transferase
Reaction(IUBMB)
[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine + [E2 NEDD8-conjugating enzyme]-L-cysteine = [E1 NEDD8-activating enzyme]-L-cysteine + [E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine
Substrate
[E1 NEDD8-activating enzyme]-S-[NEDD8 protein]-yl-L-cysteine;
[E2 NEDD8-conjugating enzyme]-L-cysteine
Product
[E1 NEDD8-activating enzyme]-L-cysteine;
[E2 NEDD8-conjugating enzyme]-S-[NEDD8-protein]-yl-L-cysteine
Comment
Some RING-type E3 ubiquitin transferases (EC 2.3.2.27) are not able to bind a substrate protein directly. Instead, they form complexes with a cullin scaffold protein and a substrate recognition module, which are known as CRL (Cullin-RING-Ligase) complexes. The cullin protein needs to be activated by the ubiquitin-like protein NEDD8 in a process known as neddylation. Like ubiquitin, the NEDD8 protein ends with two glycine residues. EC 6.2.1.64, E1 NEDD8-activating enzyme, activates NEDD8 in an ATP-dependent reaction by forming a high-energy thioester intermediate between NEDD8 and one of its cysteine residues. The activated NEDD8 is subsequently transferred to a cysteine residue of an E2 NEDD8-conjugating enzyme, and is eventually conjugated to a lysine residue of specific substrates in the presence of the appropriate E3 transferase (EC 2.3.2.32, cullin-RING-type E3 NEDD8 transferase).
History
EC 2.3.2.34 created 2020
Orthology
K10579  ubiquitin-conjugating enzyme E2 M
K10687  ubiquitin-conjugating enzyme E2 F
Genes
HSA: 140739(UBE2F) 9040(UBE2M)
PTR: 460054(UBE2F) 742993(UBE2M)
PPS: 100974415(UBE2F) 100987104(UBE2M)
GGO: 101133122(UBE2F) 101140282(UBE2M)
PON: 100431972(UBE2F) 100453660(UBE2M)
NLE: 100595356(UBE2F) 100599193(UBE2M)
MCC: 695394(UBE2F) 712206(UBE2M)
MCF: 102134194(UBE2F) 102142968(UBE2M)
CSAB: 103218168(UBE2F) 103235466(UBE2M)
CATY: 105577989(UBE2F)
PANU: 101026368(UBE2F)
RRO: 104658041(UBE2F) 104660737(UBE2M)
RBB: 108514338(UBE2M) 108531914(UBE2F) 108543663
TFN: 117070912 117091876(UBE2M) 117094328(UBE2F)
CJC: 100396395(UBE2M) 100409142(UBE2F)
SBQ: 101034041(UBE2F) 101038750(UBE2M)
MMUR: 105864498(UBE2M) 105866404(UBE2F)
MMU: 22192(Ube2m) 67921(Ube2f)
MCAL: 110291906(Ube2f) 110297241(Ube2m) 110306700
MPAH: 110321719(Ube2f) 110324792 110328573 110336512(Ube2m)
RNO: 361509(Ube2m) 363284(Ube2f)
MCOC: 116088762(Ube2f) 116101658(Ube2m)
MUN: 110554313(Ube2f) 110559547 110562936(Ube2m)
CGE: 100767306(Ube2m) 100773164(Ube2f)
PLEU: 114700686(Ube2f) 114703895(Ube2m)
NGI: 103725480(Ube2m) 103733607(Ube2f)
HGL: 101720409(Ube2f) 101725386 106007511(Ube2m)
CCAN: 109683042(Ube2m) 109693293(Ube2f)
OCU: 100341057(UBE2M) 100345408 100348202(UBE2F)
TUP: 102479857(UBE2F) 102484472(UBE2M)
CFA: 477421(UBE2F) 484221(UBE2M)
VVP: 112908778(UBE2F) 112908800(UBE2M)
VLG: 121484190(UBE2M) 121497018(UBE2F)
AML: 100465277(UBE2F) 100484638(UBE2M)
UMR: 103662623(UBE2F) 103677213(UBE2M)
UAH: 113248037(UBE2M) 113250156(UBE2F)
ORO: 101370249(UBE2F) 101382819(UBE2M) 101385331
MPUF: 101685939(UBE2F)
EJU: 114200959(UBE2M) 114210789(UBE2F)
MLX: 117997894(UBE2M) 118011894(UBE2F)
FCA: 101087858(UBE2F) 101088703(UBE2M)
PYU: 121014365(UBE2F) 121023417(UBE2M)
PBG: 122481965(UBE2F) 122494671(UBE2M)
PTG: 102954941(UBE2F) 102959074(UBE2M)
PPAD: 109260448(UBE2M) 109264837(UBE2F)
AJU: 106987929(UBE2M) 106988617(UBE2F)
HHV: 120227096(UBE2M) 120229506(UBE2F)
BTA: 613343(UBE2M) 617083(UBE2F)
BOM: 102272903(UBE2F) 102285274(UBE2M)
BIU: 109556574(UBE2F) 109571630(UBE2M)
BBUB: 102403498(UBE2M) 102403704(UBE2F)
CHX: 102173633(UBE2F) 102183722(UBE2M)
OAS: 101121449(UBE2M) 101123160(UBE2F)
ODA: 120856501(UBE2F) 120861577 120871841(UBE2M)
CCAD: 122420679(UBE2M) 122437304(UBE2F)
SSC: 100517862(UBE2F) 100621774(UBE2M)
CFR: 102520246(UBE2F) 102521589(UBE2M)
CBAI: 105063551(UBE2F) 105074459(UBE2M)
CDK: 105096792(UBE2M) 105102868(UBE2F)
BACU: 103000196(UBE2F) 103020382(UBE2M)
LVE: 103068611(UBE2F) 103087191(UBE2M)
OOR: 101272216(UBE2F) 101282617(UBE2M)
DLE: 111170063(UBE2M) 111171976(UBE2F)
PCAD: 102976571(UBE2M) 102981670(UBE2F)
ECB: 100057575(UBE2F) 100064883(UBE2M)
EPZ: 103557361(UBE2F) 103567896(UBE2M)
EAI: 106832191(UBE2F) 106846647(UBE2M)
MYB: 102239176(UBE2F) 102258559(UBE2M)
MYD: 102752745(UBE2M) 102757897(UBE2F)
MMYO: 118649860 118657433(UBE2M) 118661146(UBE2F)
MNA: 107530030(UBE2F) 107539363(UBE2M)
HAI: 109374627(UBE2F) 109377094(UBE2M)
DRO: 112307771(UBE2F) 112313630(UBE2M)
SHON: 118988194(UBE2M) 119003060(UBE2F)
AJM: 119051615(UBE2M) 119061023(UBE2F)
MMF: 118625213(UBE2F) 118638233(UBE2M)
PALE: 102892642(UBE2M) 102895482(UBE2F)
PGIG: 120601000(UBE2F) 120607146(UBE2M)
RAY: 107500478(UBE2M) 107510598(UBE2F)
MJV: 108386303(UBE2F) 108408241(UBE2M)
TOD: 119249808(UBE2M) 119256339(UBE2F)
LAV: 100668100(UBE2F) 100677054(UBE2M)
MDO: 100023491(UBE2F)
SHR: 100918650(UBE2F) 100927599(UBE2M)
PCW: 110197870(UBE2M) 110198032(UBE2F)
OAA: 100076471(UBE2F) 114814639(UBE2M)
GGA: 107049465(UBE2M) 424015(UBE2F)
PCOC: 116236051(UBE2F) 116240043(UBE2M)
MGP: 100545352(UBE2M) 100549981(UBE2F)
CJO: 107316475(UBE2F)
NMEL: 110391006(UBE2M) 110400127(UBE2F)
APLA: 101795615(UBE2F)
ACYG: 106034524(UBE2F)
TGU: 100219250(UBE2F) 115491823(UBE2M)
LSR: 110473519(UBE2F) 110481919(UBE2M)
SCAN: 103814581(UBE2F)
PMOA: 120505373(UBE2M) 120511467(UBE2F)
GFR: 102037965(UBE2F) 102040866(UBE2M)
FAB: 101814292(UBE2F)
PHI: 102100393(UBE2M) 102112379(UBE2F)
PMAJ: 107207593(UBE2F)
CCAE: 111931865(UBE2F)
CCW: 104694551(UBE2F) 120412272(UBE2M)
ETL: 114060651(UBE2F) 114072657(UBE2M)
FPG: 101922503(UBE2F) 101924990(UBE2M)
FCH: 102052086(UBE2M) 102053054(UBE2F)
CLV: 102089847 102093842(UBE2F)
EGZ: 104123244(UBE2F)
NNI: 104009968(UBE2F)
ACUN: 113482146(UBE2F)
PADL: 103921638(UBE2F)
AAM: 106492477(UBE2F) 106497830(UBE2M)
AROW: 112976180(UBE2F)
NPD: 112955699(UBE2F)
DNE: 112995956(UBE2M) 112996033(UBE2F)
ASN: 102372051(UBE2F) 102387524(UBE2M)
AMJ: 102562327(UBE2M) 102570819(UBE2F)
CPOO: 109311667(UBE2F)
GGN: 109301934(UBE2F)
PSS: 102452238(UBE2F) 102462380(UBE2M)
CMY: 102943228(UBE2F) 102944014(UBE2M)
CPIC: 101933458(UBE2F) 101944436(UBE2M)
TST: 117884758(UBE2F) 117887114(UBE2M)
CABI: 116839416(UBE2F)
ACS: 100559377(ube2f) 100561385(ube2m)
PVT: 110081891(UBE2F) 110091319(UBE2M)
PBI: 103050957(UBE2F) 103067550(UBE2M)
PMUR: 107282388(UBE2M) 107295129 107300190 107302998(UBE2F)
TSR: 106541855(UBE2F) 106542185(UBE2M) 106548057
PGUT: 117669037(UBE2M) 117670775(UBE2F)
PMUA: 114582406(UBE2M) 114583211(UBE2F)
ZVI: 118095092(UBE2M) 118097640(UBE2F)
GJA: 107121185(UBE2F) 107122671(UBE2M)
XLA: 108697716(ube2m.S) 108701421 432085(ube2f.S) 446800(ube2m.L)
XTR: 394553(ube2m) 496657(ube2f)
NPR: 108789832(UBE2F) 108802228(UBE2M)
DRE: 406606(ube2f) 564377(si:ch1073-205c8.3)
IPU: 108257490(ube2m) 108258865(ube2f)
PHYP: 113535821(ube2f)
AMEX: 103027775(ube2f) 103036171(ube2m)
EEE: 113581154(ube2m) 113588844(ube2f)
TRU: 101069584(ube2f) 105418275(ube2m)
LCO: 104923141(ube2f) 104936300(ube2m)
NCC: 104955814(ube2f)
CGOB: 115026599(ube2f)
ELY: 117251456(ube2f) 117268617(ube2m)
PLEP: 121949030(ube2f) 121956821(ube2m)
SLUC: 116044461(ube2f) 116062293(ube2m)
ECRA: 117952830(ube2f)
PFLV: 114564052(ube2f) 114569626(ube2m)
GAT: 120819820 120828068(ube2m) 120833788(ube2f)
MSAM: 119896595(ube2m) 119903864(ube2f)
CUD: 121522588(ube2f) 121529083(ube2m)
MZE: 101467147(ube2f) 101474703(ube2m)
ONL: 100696605(ube2m) 100699014(ube2f)
OAU: 116311519(ube2m) 116325706(ube2f)
OLA: 101158243(ube2f) 111947719(ube2m)
OML: 112139410(ube2m) 112155142(ube2f)
XMA: 102219600(ube2m) 102231843(ube2f)
XCO: 114148651(ube2f) 114160054(ube2m)
XHE: 116723432(ube2f) 116735673(ube2m)
PRET: 103460261(ube2f) 103469463(ube2m)
CVG: 107094136(ube2f) 107097644(ube2m)
CTUL: 119796540(ube2f) 119796895(ube2m)
NFU: 107372565 107389395(ube2f) 107395197(ube2m)
KMR: 108229416(ube2m) 108238496(ube2f)
ALIM: 106528063(ube2m) 106529439(ube2f)
AOCE: 111563048(ube2m) 111573017(ube2f)
CSEM: 103392467(ube2f) 103393415(ube2m)
POV: 109631209 109645556(ube2m)
LCF: 108887096(ube2f) 108891035(ube2m)
SDU: 111218665(ube2f) 111226830(ube2m)
SLAL: 111646356(ube2m) 111667442(ube2f)
XGL: 120788341(ube2m) 120801550(ube2f)
HCQ: 109512318(ube2f) 109513758(ube2m)
BPEC: 110170932(ube2f)
MALB: 109968559(ube2f) 109973439(ube2m)
SASA: 100380794(ubc12) 100380844(ube2f) 106582389 106600804
ELS: 105013286(ube2m) 105026253(ube2f)
SFM: 108929010(ube2f) 108933567(ube2m)
PKI: 111850204(ube2f) 111850823(ube2m)
AANG: 118217395(ube2m) 118224376(ube2f)
LOC: 102685546(ube2f)
ARUT: 117400649(ube2f) 117407745(ube2m) 117426447 117967268
LCM: 102361697(UBE2F) 102367640(UBE2M)
CMK: 103171625(ube2m) 103187934(ube2f)
RTP: 109911060(ube2f) 109919636(ube2m)
DME: Dmel_CG7375(UbcE2M)
DER: 6544398
DSE: 6604834
DSI: Dsimw501_GD13010(Dsim_GD13010)
DAN: 6507331
DSR: 110183632
DPE: 6599481
DMN: 108152462
DWI: 6645404
DGR: 6558845
DAZ: 108614001
DNV: 108652064
DHE: 111592838
DVI: 6622137
CCAT: 101453065
MDE: 101900864
LCQ: 111690006
API: 100160864
DNX: 107166515
AGS: 114121238
RMD: 113550151
DPTE: 113792009
CEL: CELE_R09B3.4(ubc-12)
CBR: CBG11949 CBG18691(Cbr-ubc-12)
BMY: BM_BM13724(Bma-ubc-12)
TSP: Tsp_01616
EGL: EGR_00111
ATH: AT2G18600 AT4G36800(RCE1)
CCAJ: 109800720
APRC: 113870305
CAM: 101490453
LJA: Lj2g3v1803100.1(Lj2g3v1803100.1)
ADU: 107487884
AIP: 107642845
RCU: 8264183
EGT: 105965696
BVG: 104904391
SOE: 110800374
NCOL: 116266101
DOSA: Os08t0374100-01(Os08g0374100) Os09t0321900-01(Os09g0321900) Os10t0190000-01(Os10g0190000)
DCT: 110107924
ATR: 18445913
MNG: MNEG_6166
APRO: F751_6769
SCE: YLR306W(UBC12)
ERC: Ecym_8371
KMX: KLMA_30316(UBC12)
NCS: NCAS_0A05940(NCAS0A05940)
NDI: NDAI_0D02910(NDAI0D02910)
TPF: TPHA_0F00390(TPHA0F00390)
TBL: TBLA_0E01570(TBLA0E01570)
TDL: TDEL_0E05560(TDEL0E05560)
KAF: KAFR_0F03040(KAFR0F03040)
CAL: CAALFM_C300520WA(CaO19.5411)
NCR: NCU03610
NTE: NEUTE1DRAFT58553(NEUTE1DRAFT_58553)
MGR: MGG_01807
SSCK: SPSK_07977
MAW: MAC_03819
MAJ: MAA_02998
CMT: CCM_01981
MBE: MBM_08060
ANG: ANI_1_496084(An09g04000)
ABE: ARB_00725
TVE: TRV_00178
PTE: PTT_14980
SPO: SPCC777.10c(ubc12)
CNE: CNJ01020
CNB: CNBJ2460
TASA: A1Q1_06417
ABP: AGABI1DRAFT110695(AGABI1DRAFT_110695)
ABV: AGABI2DRAFT189402(AGABI2DRAFT_189402)
MGL: MGL_3956
MRT: MRET_2805
DDI: DDB_G0281725(ube2m)
DFA: DFA_09823(ube2m)
EHI: EHI_003010(98.t00007)
PCB: PCHAS_146080(PC000397.01.0)
TAN: TA05945
TPV: TP01_0844
BBO: BBOV_IV009300(23.m06353)
CPV: cgd8_220
SMIN: v1.2.037828.t1(symbB.v1.2.037828.t1)
 » show all
Reference
1  [PMID:9694792]
  Authors
Osaka F, Kawasaki H, Aida N, Saeki M, Chiba T, Kawashima S, Tanaka K, Kato S
  Title
A new NEDD8-ligating system for cullin-4A.
  Journal
Genes Dev 12:2263-8 (1998)
DOI:10.1101/gad.12.15.2263
  Sequence
[hsa:9040]
Reference
2  [PMID:10207026]
  Authors
Gong L, Yeh ET
  Title
Identification of the activating and conjugating enzymes of the NEDD8 conjugation pathway.
  Journal
J Biol Chem 274:12036-42 (1999)
DOI:10.1074/jbc.274.17.12036
Reference
3  [PMID:15361859]
  Authors
Huang DT, Miller DW, Mathew R, Cassell R, Holton JM, Roussel MF, Schulman BA
  Title
A unique E1-E2 interaction required for optimal conjugation of the ubiquitin-like protein NEDD8.
  Journal
Nat Struct Mol Biol 11:927-35 (2004)
DOI:10.1038/nsmb826
  Sequence
[hsa:9040]
Reference
4  [PMID:19250909]
  Authors
Huang DT, Ayrault O, Hunt HW, Taherbhoy AM, Duda DM, Scott DC, Borg LA, Neale G, Murray PJ, Roussel MF, Schulman BA
  Title
E2-RING expansion of the NEDD8 cascade confers specificity to cullin modification.
  Journal
Mol Cell 33:483-95 (2009)
DOI:10.1016/j.molcel.2009.01.011
  Sequence
[hsa:140739]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.2.34
IUBMB Enzyme Nomenclature: 2.3.2.34
ExPASy - ENZYME nomenclature database: 2.3.2.34
BRENDA, the Enzyme Database: 2.3.2.34

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