KEGG   ENZYME: 2.3.2.35
Entry
EC 2.3.2.35                 Enzyme                                 

Name
capsaicin synthase;
CS (gene name) (ambiguous);
Pun1 (locus name)
Class
Transferases;
Acyltransferases;
Aminoacyltransferases
Sysname
(6E)-8-methylnon-6-enoyl-CoA:vanillylamine 8-methylnon-6-enoyltransferase
Reaction(IUBMB)
(6E)-8-methylnon-6-enoyl-CoA + vanillylamine = CoA + capsaicin [RN:R12626]
Reaction(KEGG)
R12626
Substrate
(6E)-8-methylnon-6-enoyl-CoA [CPD:C22289];
vanillylamine [CPD:C16666]
Product
CoA [CPD:C00010];
capsaicin [CPD:C06866]
Comment
The enzyme, found only in plants that belong to the Capsicum genus, catalyses the last step in the biosynthesis of capsaicinoids. The enzyme catalyses the acylation of vanillylamine by a branched-chain fatty acid. The exact structure of the fatty acid determines the type of capsaicinoid formed.
History
EC 2.3.2.35 created 2020
Pathway
ec00998  Biosynthesis of various secondary metabolites - part 2
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K24277  capsaicin synthase
Genes
SLY: 101255851 101256149
SPEN: 107010618
SOT: 102597608
CANN: 107859694(AT3-1)
ITR: 116026080 116026905
Reference
1  [PMID:12175073]
  Authors
Blum E, Liu K, Mazourek M, Yoo EY, Jahn M, Paran I
  Title
Molecular mapping of the C locus for presence of pungency in Capsicum.
  Journal
Genome 45:702-5 (2002)
DOI:10.1139/g02-031
Reference
2  [PMID:15918882]
  Authors
Stewart C Jr, Kang BC, Liu K, Mazourek M, Moore SL, Yoo EY, Kim BD, Paran I, Jahn MM
  Title
The Pun1 gene for pungency in pepper encodes a putative acyltransferase.
  Journal
Plant J 42:675-88 (2005)
DOI:10.1111/j.1365-313X.2005.02410.x
  Sequence
[cann:107859694]
Reference
3  [PMID:24441736]
  Authors
Kim S, Park M, Yeom SI, Kim YM, Lee JM, Lee HA, Seo E, Choi J, Cheong K, Kim KT, Jung K, Lee GW, Oh SK, Bae C, Kim SB, Lee HY, Kim SY, Kim MS, Kang BC, Jo YD, Yang HB, Jeong HJ, Kang WH, Kwon JK, Shin C, Lim JY, Park JH, Huh JH, Kim JS, Kim BD, Cohen O, Paran I, Suh MC, Lee SB, Kim YK, Shin Y, Noh SJ, Park J, Seo YS, Kwon SY, Kim HA, Park JM, Kim HJ, Choi SB, Bosland PW, Reeves G, Jo SH, Lee BW, Cho HT, Choi HS, Lee MS, Yu Y, Do Choi Y, Park BS, van Deynze A, Ashrafi H, Hill T, Kim WT, Pai HS, Ahn HK, Yeam I, Giovannoni JJ, Rose JK, Sorensen I, Lee SJ, Kim RW, Choi IY, Choi BS, Lim JS, Lee YH, Choi D
  Title
Genome sequence of the hot pepper provides insights into the evolution of pungency in Capsicum species.
  Journal
Nat Genet 46:270-8 (2014)
DOI:10.1038/ng.2877
Other DBs
ExplorEnz - The Enzyme Database: 2.3.2.35
IUBMB Enzyme Nomenclature: 2.3.2.35
ExPASy - ENZYME nomenclature database: 2.3.2.35
BRENDA, the Enzyme Database: 2.3.2.35

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