KEGG   ENZYME: 2.3.3.17
Entry
EC 2.3.3.17                 Enzyme                                 

Name
methylthioalkylmalate synthase;
MAM1 (gene name);
MAM3 (gene name);
acetyl-CoA:omega-(methylthio)-2-oxoalkanoate C-acetyltransferase
Class
Transferases;
Acyltransferases;
Acyl groups converted into alkyl groups on transfer
Sysname
acetyl-CoA:omega-(methylsulfanyl)-2-oxoalkanoate C-acetyltransferase
Reaction(IUBMB)
an omega-(methylsulfanyl)-2-oxoalkanoate + acetyl-CoA + H2O = a 2-[omega-(methylsulfanyl)alkyl]malate + CoA
Reaction(KEGG)
Substrate
omega-(methylsulfanyl)-2-oxoalkanoate;
acetyl-CoA [CPD:C00024];
H2O [CPD:C00001]
Product
2-[omega-(methylsulfanyl)alkyl]malate;
CoA [CPD:C00010]
Comment
The enzyme, characterized from the plant Arabidopsis thaliana, is involved in the L-methionine side-chain elongation pathway, forming substrates for the biosynthesis of aliphatic glucosinolates. Two forms are known - MAM1 catalyses only only the first two rounds of methionine chain elongation, while MAM3 catalyses all six cycles, up to formation of L-hexahomomethionine.
History
EC 2.3.3.17 created 2016
Pathway
ec00966  Glucosinolate biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K15741  methylthioalkylmalate synthase 1
K15742  methylthioalkylmalate synthase 3
Genes
HAZT: 108682265
ATH: AT5G23010(MAM1) AT5G23020(IMS2)
ALY: 9308098 9308099 9308100
CRB: 17881318 17883723 17883860
CSAT: 104706601 104706602 104706603 104736315 104736316 104736317 104770650 104770651 104770652
EUS: EUTSA_v10003976mg EUTSA_v10004072mg EUTSA_v10005522mg
BRP: 103853322 103854915 103860537(MAM3-1) 103860538(MAM1-1) 103861112
BNA: 106375002 106377268 106377269 106382716 106434594 106434595 106434598 106434605 106444270 111203418 111205591
BOE: 106301761 106324531 106324637 106325524
RSZ: 108813467 108828349 108832673 108835158 108848454
Reference
1  [PMID:14740211]
  Authors
Textor S, Bartram S, Kroymann J, Falk KL, Hick A, Pickett JA, Gershenzon J
  Title
Biosynthesis of methionine-derived glucosinolates in Arabidopsis thaliana: recombinant expression and characterization of methylthioalkylmalate synthase, the condensing enzyme of the chain-elongation cycle.
  Journal
Planta 218:1026-35 (2004)
DOI:10.1007/s00425-003-1184-3
  Sequence
[ath:AT5G23010]
Reference
2  [PMID:17369439]
  Authors
Textor S, de Kraker JW, Hause B, Gershenzon J, Tokuhisa JG
  Title
MAM3 catalyzes the formation of all aliphatic glucosinolate chain lengths in Arabidopsis.
  Journal
Plant Physiol 144:60-71 (2007)
DOI:10.1104/pp.106.091579
  Sequence
[ath:AT5G23020]
Other DBs
ExplorEnz - The Enzyme Database: 2.3.3.17
IUBMB Enzyme Nomenclature: 2.3.3.17
ExPASy - ENZYME nomenclature database: 2.3.3.17
BRENDA, the Enzyme Database: 2.3.3.17

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