KEGG   ENZYME: 2.4.1.246
Entry
EC 2.4.1.246                Enzyme                                 
Name
mannosylfructose-phosphate synthase;
mannosylfructose-6-phosphate synthase;
MFPS
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
GDP-mannose:D-fructose-6-phosphate 2-alpha-D-mannosyltransferase
Reaction(IUBMB)
GDP-mannose + D-fructose 6-phosphate = GDP + beta-D-fructofuranosyl-alpha-D-mannopyranoside 6F-phosphate [RN:R08947]
Reaction(KEGG)
R08947
Substrate
GDP-mannose [CPD:C00096];
D-fructose 6-phosphate [CPD:C00085]
Product
GDP [CPD:C00035];
beta-D-fructofuranosyl-alpha-D-mannopyranoside 6F-phosphate [CPD:C18050]
Comment
This enzyme, from the soil proteobacterium and plant pathogen Agrobacterium tumefaciens strain C58, requires Mg2+ or Mn2+ for activity. GDP-mannose can be replaced by ADP-mannose but with a concomitant decrease in activity. The product of this reaction is dephosphorylated by EC 3.1.3.79 (mannosylfructose-phosphate phosphatase) to form the non-reducing disaccharide mannosylfructose, which is the major endogenous osmolyte produced by several alpha-proteobacteria in response to osmotic stress. The F in the product name is used to indicate that the fructose residue of sucrose carries the substituent.
History
EC 2.4.1.246 created 2008
Orthology
K13058  mannosylfructose-phosphate synthase
Genes
MESMEJ066_25975
ATUAtu0661
ATAAWN88_22935
AGRAGROH133_04182
ATFAch5_05810
AGCBSY240_1856(mfpsA)
AROB0909_10205
AGTEYD00_01525
ALFCFBP5473_12495
ARUIG6M88_10360
AVQHRR99_02170
RIRBN877_I0631(mfpsA)
RPUSCFBP5875_01810
RHVBA939_12235
ROYG3A56_07800
PDESFE840_007985
MEYTM49_21055
MAADAZF01_03530
MMEDMame_02236(mfpsA)
MLUTJET14_04550
LAPACP90_19440
LABRCHH27_00890
LABTFIU93_25995(mfpsA1)
LAGGB0E33_03120
OTEOter_3275
VBHCMV30_07430
OLEK0B96_13030
OBTOPIT5_27835
RUFIK0V07_16125
LUOHHL09_01615
PSLPsta_2175
LCREPla8534_51990(mfpsA)
PHMPSMK_07010
MCADPan265_24610(mfpsA)
SSMSpirs_0903
SLRL21SP2_2636
POBLPB03_05960
PRNBW723_17535
PLITK8354_01845
SZEAW14_07280
AHZAPS56_07825
TAJC1A40_11730
MARFCJ739_416
 » show all
Reference
1  [PMID:17728402]
  Authors
Torres LL, Salerno GL
  Title
A metabolic pathway leading to mannosylfructose biosynthesis in Agrobacterium tumefaciens uncovers a family of mannosyltransferases.
  Journal
Proc Natl Acad Sci U S A 104:14318-23 (2007)
DOI:10.1073/pnas.0706709104
  Sequence
[atu:Atu0661]
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.246
IUBMB Enzyme Nomenclature: 2.4.1.246
ExPASy - ENZYME nomenclature database: 2.4.1.246
BRENDA, the Enzyme Database: 2.4.1.246
CAS: 92480-04-1

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