KEGG   ENZYME: 2.4.1.309
Entry
EC 2.4.1.309                Enzyme                                 

Name
UDP-Gal:alpha-L-Fuc-1,2-beta-Gal-1,3-alpha-GalNAc-1,3-alpha-GalNAc-diphosphoundecaprenol alpha-1,3-galactosyltransferase;
WbnI
Class
Transferases;
Glycosyltransferases;
Hexosyltransferases
Sysname
UDP-alpha-D-galactose:alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol alpha-1,3-galactosyltransferase
Reaction(IUBMB)
UDP-alpha-D-galactose + alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol = UDP + alpha-D-Gal-(1->3)-(alpha-L-Fuc-(1->2))-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol [RN:R10440]
Reaction(KEGG)
R10440
Substrate
UDP-alpha-D-galactose [CPD:C00052];
alpha-L-Fuc-(1->2)-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol [CPD:C20625]
Product
UDP [CPD:C00015];
alpha-D-Gal-(1->3)-(alpha-L-Fuc-(1->2))-beta-D-Gal-(1->3)-alpha-D-GalNAc-(1->3)-alpha-D-GalNAc-diphospho-ditrans,octacis-undecaprenol [CPD:C20626]
Comment
The enzyme is involved in the the biosynthesis of the O-polysaccharide repeating unit of the bacterium Escherichia coli serotype O86.
History
EC 2.4.1.309 created 2013
Orthology
K21368  O86-antigen biosynthesis alpha-1,3-galactosyltransferase
Reference
1  [PMID:15713070]
  Authors
Yi W, Shao J, Zhu L, Li M, Singh M, Lu Y, Lin S, Li H, Ryu K, Shen J, Guo H, Yao Q, Bush CA, Wang PG
  Title
Escherichia coli O86 O-antigen biosynthetic gene cluster and stepwise enzymatic synthesis of human blood group B antigen tetrasaccharide.
  Journal
J Am Chem Soc 127:2040-1 (2005)
DOI:10.1021/ja045021y
  Sequence
Reference
2  [PMID:16839526]
  Authors
Yi W, Zhu L, Guo H, Li M, Li J, Wang PG
  Title
Formation of a new O-polysaccharide in Escherichia coli O86 via disruption of a glycosyltransferase gene involved in O-unit assembly.
  Journal
Carbohydr Res 341:2254-60 (2006)
DOI:10.1016/j.carres.2006.06.009
Reference
3  [PMID:20418877]
  Authors
Woodward R, Yi W, Li L, Zhao G, Eguchi H, Sridhar PR, Guo H, Song JK, Motari E, Cai L, Kelleher P, Liu X, Han W, Zhang W, Ding Y, Li M, Wang PG
  Title
In vitro bacterial polysaccharide biosynthesis: defining the functions of Wzy and Wzz.
  Journal
Nat Chem Biol 6:418-23 (2010)
DOI:10.1038/nchembio.351
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.4.1.309
IUBMB Enzyme Nomenclature: 2.4.1.309
ExPASy - ENZYME nomenclature database: 2.4.1.309
BRENDA, the Enzyme Database: 2.4.1.309

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