KEGG   ENZYME: 2.5.1.30
Entry
EC 2.5.1.30                 Enzyme                                 

Name
heptaprenyl diphosphate synthase;
all-trans-heptaprenyl-diphosphate synthase;
heptaprenyl pyrophosphate synthase;
heptaprenyl pyrophosphate synthetase;
HepPP synthase;
HepPS;
heptaprenylpyrophosphate synthetase
Class
Transferases;
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
Sysname
(2E,6E)-farnesyl-diphosphate:isopentenyl-diphosphate farnesyltranstransferase (adding 4 isopentenyl units)
Reaction(IUBMB)
(2E,6E)-farnesyl diphosphate + 4 isopentenyl diphosphate = 4 diphosphate + all-trans-heptaprenyl diphosphate [RN:R09247]
Reaction(KEGG)
R09247
Substrate
(2E,6E)-farnesyl diphosphate [CPD:C00448];
isopentenyl diphosphate [CPD:C00129]
Product
diphosphate [CPD:C00013];
all-trans-heptaprenyl diphosphate [CPD:C04216]
Comment
This enzyme catalyses the condensation reactions resulting in the formation of all-trans-heptaprenyl diphosphate, the isoprenoid side chain of ubiquinone-7 and menaquinone-7. The enzyme adds four isopentenyl diphosphate molecules sequentially to farnesyl diphosphate with trans stereochemistry.
History
EC 2.5.1.30 created 1984, modified 2010
Pathway
ec00900  Terpenoid backbone biosynthesis
ec01110  Biosynthesis of secondary metabolites
Orthology
K00805  heptaprenyl diphosphate synthase component 1
K24873  heptaprenyl diphosphate synthase component 2
Genes
SDO: SD1155_09465 SD1155_09475
BSU: BSU22740(hepT) BSU22760(hepS)
BSR: I33_2334(hepT) I33_2336
BSL: A7A1_2963 A7A1_2965
BSH: BSU6051_22740(hepT) BSU6051_22760(hepS)
BSY: I653_10855 I653_10865
BSUT: BSUB_02441(hepT) BSUB_02443(hepS)
BSUL: BSUA_02441(hepT) BSUA_02443(hepS)
BSUS: Q433_12390 Q433_12400
BSO: BSNT_08671(hepT) BSNT_08673(hepS)
BSQ: B657_22740(hepT) B657_22760(hepS)
BSX: C663_2149(hepS)
BSS: BSUW23_11155(hepT) BSUW23_11165(hepS)
BST: GYO_2502(hepT) GYO_2504
BLI: BL02782(hepT) BL02784(hepS)
BLD: BLi02409(hepT) BLi02411(hepS)
BLH: BaLi_c24980(hepT) BaLi_c25000(hepS)
BAQ: BACAU_2098(hepT) BACAU_2100(hepS)
BYA: BANAU_2241(hepT) BANAU_2243(hepS)
BAML: BAM5036_2017(hepT) BAM5036_2019(hepS)
BAMA: RBAU_2229(hepT) RBAU_2231(hepS)
BAMN: BASU_2018(hepT) BASU_2020(hepS)
BAMB: BAPNAU_1495(hepS) BAPNAU_1497(hepT)
BAMY: V529_23580(hepT) V529_23600(hepS)
BMP: NG74_02189(hepT) NG74_02191(hepS)
BAO: BAMF_2175(hepT) BAMF_2177(hepS)
BAZ: BAMTA208_05585(hepS) BAMTA208_05595(hepT)
BQL: LL3_02459(hepT) LL3_02461(hepS)
BXH: BAXH7_01167(hepS) BAXH7_01169(hepT)
BQY: MUS_2523(hepT) MUS_2525(hepS)
BMOJ: HC660_21540(hepT) HC660_21560(hepS)
BAN: BA_1533 BA_1535(hepT)
BAR: GBAA_1533 GBAA_1535(hepT)
BAI: BAA_1602 BAA_1604(hepT)
BANV: DJ46_355(hepS) DJ46_357(hepT)
BCA: BCE_1639 BCE_1641(hepT)
BCZ: BCE33L1394(hepS) BCE33L1396(hepT)
BCQ: BCQ_1581(hepS) BCQ_1583(hepT)
BCX: BCA_1571 BCA_1573(hepT)
BAL: BACI_c15550(hepS) BACI_c15570(hepT)
BTK: BT9727_1394(hepS) BT9727_1396(hepT)
BTL: BALH_1366 BALH_1368(hepT)
BTG: BTB_c15530(hepS) BTB_c15550(hepT)
BTW: BF38_2731(hepS) BF38_2733(hepT)
BWW: bwei_3478(hepT) bwei_3480(hepS)
BMYO: BG05_4377(hepT) BG05_4379
BMYC: DJ92_4161 DJ92_4163(hepT)
BMET: BMMGA3_10760(hepT) BMMGA3_10770(hepS)
BACL: BS34A_25030(hepT) BS34A_25050(hepS)
BGY: BGLY_2684(hepT) BGLY_2686(hepS)
BBEV: BBEV_1441(hepT)
BFD: NCTC4823_02031(hepS) NCTC4823_02033(hepT)
BMQ: BMQ_4328(hepT) BMQ_4330(hepS)
BMD: BMD_4315(hepT) BMD_4317(hepS)
BMH: BMWSH_0902(hepS) BMWSH_0904(hepT)
BMEG: BG04_1247(hepT) BG04_1249
BCOA: BF29_608(hepT) BF29_610
BHA: BH1648(gerCA) BH1653(gerCC)
BCL: ABC1885 ABC1889(hepA)
BPF: BpOF4_15395(hepS) BpOF4_15420(hepT)
BKW: BkAM31D_10955(hepS) BkAM31D_10980(hepT)
OIH: OB1788(gerCC) OB1790(gerCA)
GKA: GK2210(gerCC) GK2212(gerCA)
GGH: GHH_c22990(hepT) GHH_c23010(hepS)
GEA: GARCT_02187(hepT) GARCT_02189(hepS)
PCAL: BV455_03200(hepS) BV455_03202(hepT)
AFL: Aflv_1093 Aflv_1095(hepT)
ANM: GFC28_141 GFC28_143(hepT)
AAMY: GFC30_2409(hepT) GFC30_2411(hepS)
LSP: Bsph_1932(gerCA) Bsph_1934
HHD: HBHAL_3282(hepT) HBHAL_3284(hepS)
HLI: HLI_00460 HLI_00470(hepT)
VPN: A21D_03370(hepS) A21D_03372(hepT)
VIM: GWK91_06330(hepT)
PASA: BAOM_3531(hepST) BAOM_3533(hepST)
PSYO: PB01_07585 PB01_07595(hepT)
GRC: GI584_12455(hepT)
SALE: EPH95_01185(hepT)
BLEN: NCTC4824_01941(hepS) NCTC4824_01943(hepT)
BSE: Bsel_2132
SAU: SA1302(gerCC) SA1304
SAV: SAV1470(gerCC) SAV1472
SAW: SAHV_1458(gerCC) SAHV_1460
SAM: MW1359(gerCC) MW1361
SAE: NWMN_1379(hepT) NWMN_1381
SUZ: MS7_1424(hepT) MS7_1426
SAUE: RSAU_001340(gerCC) RSAU_001342
SAUC: CA347_1406(hepT) CA347_1408
SAUR: SABB_00045 SABB_00047(hepT)
SEPS: DP17_120(hepT) DP17_122
SHA: SH1442 SH1444(gerCC)
SHH: ShL2_01325 ShL2_01327(gerCC)
SCA: SCA_1102(gerCC) SCA_1104
LMOL: LMOL312_1940(hepT) LMOL312_1942(hepS)
LMQ: LMM7_2023(ispB) LMM7_2025
LML: lmo4a_1987(hepT) lmo4a_1989(hepS)
LWE: lwe1956(hepB) lwe1958(hepS)
LSG: lse_1916(hepB) lse_1918
LGZ: NCTC10812_00886(hepS) NCTC10812_00888(hepT_1)
EAN: Eab7_1648(hepT) Eab7_1650(hepS)
BBE: BBR47_24620(hepS) BBR47_24670(hepB)
PPM: PPSC2_14605(hepT) PPSC2_14630(hepS)
PPO: PPM_2936(hepT) PPM_2941(hepS)
PRI: PRIO_4477(hepT) PRIO_4482
ASOC: CB4_03073(hepT) CB4_03078(hepS)
AAC: Aaci_1694
AAD: TC41_1590
KZO: NCTC404_01702(hepS) NCTC404_01704(hepT)
SWO: Swol_0680
SLP: Slip_1436
SALQ: SYNTR_0453
DSY: DSY2232
DHD: Dhaf_3361
DRM: Dred_2166
DAE: Dtox_1248
PTH: PTH_0969(IspA)
DAU: Daud_1350
SGY: Sgly_1713
HMO: HM1_1960
HCV: FTV88_1664(hepT)
CTHM: CFE_1715
CHY: CHY_1206(hepT)
MTA: Moth_1252
MTHO: MOTHE_c12330(hepT2)
MTHZ: MOTHA_c13190(hepT2)
ADG: Adeg_1262
TPZ: Tph_c15430(hepT2)
MSHO: MSHO_05940
ACIT: HPK19_02915(hepT)
ASER: Asera_34000(ispB)
 » show all
Reference
1  [PMID:6768722]
  Authors
Takahashi I, Ogura K, Seto S.
  Title
Heptaprenyl pyrophosphate synthetase from Bacillus subtilis.
  Journal
J Biol Chem 255:4539-43 (1980)
Reference
2  [PMID:9748348]
  Authors
Zhang YW, Koyama T, Marecak DM, Prestwich GD, Maki Y, Ogura K
  Title
Two subunits of heptaprenyl diphosphate synthase of Bacillus subtilis form a catalytically active complex.
  Journal
Biochemistry 37:13411-20 (1998)
DOI:10.1021/bi972926y
Reference
3  [PMID:10545188]
  Authors
Zhang YW, Li XY, Sugawara H, Koyama T
  Title
Site-directed mutagenesis of the conserved residues in component I of Bacillus subtilis heptaprenyl diphosphate synthase.
  Journal
Biochemistry 38:14638-43 (1999)
DOI:10.1021/bi9913653
Reference
4  [PMID:17117872]
  Authors
Suzuki T, Zhang YW, Koyama T, Sasaki DY, Kurihara K
  Title
Direct observation of substrate-enzyme complexation by surface forces measurement.
  Journal
J Am Chem Soc 128:15209-14 (2006)
DOI:10.1021/ja061822k
Other DBs
ExplorEnz - The Enzyme Database: 2.5.1.30
IUBMB Enzyme Nomenclature: 2.5.1.30
ExPASy - ENZYME nomenclature database: 2.5.1.30
BRENDA, the Enzyme Database: 2.5.1.30
CAS: 74506-59-5

DBGET integrated database retrieval system