glyceraldehyde-3-phosphate:L-arginine 2-N-(2-hydroxy-3-oxopropyl) transferase (2-carboxyethyl-forming)
Transferring alkyl or aryl groups, other than methyl groups;
Transferring alkyl or aryl groups, other than methyl groups (only sub-subclass identified to date)
glyceraldehyde-3-phosphate:L-arginine N2-(2-hydroxy-3-oxopropyl) transferase (2-carboxyethyl-forming)
D-glyceraldehyde 3-phosphate + L-arginine = N2-(2-carboxyethyl)-L-arginine + phosphate [RN:
D-glyceraldehyde 3-phosphate [CPD:
The enzyme requires thiamine diphosphate and catalyses the first step in the clavulanic-acid-biosynthesis pathway. The 2-hydroxy-3-oxo group transferred from glyceraldehyde 3-phosphate is isomerized during transfer to form the 2-carboxyethyl group.
EC 188.8.131.52 created 2004
ec00331 Clavulanic acid biosynthesis
ec01110 Biosynthesis of secondary metabolites
K12673 N2-(2-carboxyethyl)arginine synthase
SCLF: BB341_07805 BB341_29465 » show all
Caines ME, Elkins JM, Hewitson KS, Schofield CJ.
Crystal structure and mechanistic implications of N2-(2-carboxyethyl)arginine synthase, the first enzyme in the clavulanic acid biosynthesis pathway.
Khaleeli, N., Li, R. and Townsend, C.A.
Origin of the beta-lactam carbons in clavulanic acid from an unusual thiamine pyrophosphate-mediated reaction.
J Am Chem Soc 121:9223-9224 (1999)
ExplorEnz - The Enzyme Database: 184.108.40.206
ExPASy - ENZYME nomenclature database: 220.127.116.11