KEGG   ENZYME: 2.6.1.86
Entry
EC 2.6.1.86                 Enzyme                                 
Name
2-amino-4-deoxychorismate synthase;
ADIC synthase;
2-amino-2-deoxyisochorismate synthase;
SgcD
Class
Transferases;
Transferring nitrogenous groups;
Transaminases
Sysname
(2S)-2-amino-4-deoxychorismate:2-oxoglutarate aminotransferase
Reaction(IUBMB)
(2S)-2-amino-4-deoxychorismate + L-glutamate = chorismate + L-glutamine [RN:R08956]
Reaction(KEGG)
R08956
Substrate
(2S)-2-amino-4-deoxychorismate [CPD:C18054];
L-glutamate [CPD:C00025]
Product
chorismate [CPD:C00251];
L-glutamine [CPD:C00064]
Comment
Requires Mg2+. The reaction occurs in the reverse direction to that shown above. In contrast to most anthranilate-synthase I (ASI) homologues, this enzyme is not inhibited by tryptophan. In Streptomyces globisporus, the sequential action of this enzyme and EC 1.3.99.24, 2-amino-4-deoxychorismate dehydrogenase, leads to the formation of the benzoxazolinate moiety of the enediyne antitumour antibiotic C-1027 [1,2]. In certain Pseudomonads the enzyme participates in the biosynthesis of phenazine, a precursor for several compounds with antibiotic activity [3,4].
History
EC 2.6.1.86 created 2008
Pathway
ec00405  Phenazine biosynthesis
ec01059  Biosynthesis of enediyne antibiotics
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K13063  2-amino-4-deoxychorismate synthase
K20159  2-amino-4-deoxychorismate synthase
K21175  2-amino-4-deoxychorismate synthase, glutamine amidotransferase component
Genes
KOCAB185_20690
KGRJJJ10_13555
SFWWN53_03385
ECAECA2701(ehpC)
PATREV46_13195
PATOGZ59_18590(ehpC)
PPAVLOZ86_13695
XDOXDD1_2436(phzE)
XPOXPG1_2291(tomD)
XHOA9255_10780
LABLA76x_3333
LAQGLA29479_2366(phzE)
PAEPA1903(phzE2) PA4214(phzE1)
PAEVN297_1963 N297_4346
PAEIN296_1963 N296_4346
PAUPA14_09440(phzE1) PA14_39910(phzE2)
PAPPSPA7_0885 PSPA7_3381
PAGPLES_07131(phzE1) PLES_34211(phzE2)
PAFPAM18_0723(phzE1) PAM18_3139(phzE2)
PNCNCGM2_2820(phzE2) NCGM2_5459(phzE2)
PAEBNCGM1900_0745(phzE2) NCGM1900_4586(phzE2)
PDKPADK2_03600 PADK2_16135
PSGG655_03635 G655_15600
PRPM062_22200
PAEPPA1S_03750 PA1S_16425
PAERPA1R_gp2105 PA1R_gp5532
PAEMU769_03780 U769_16140
PAELT223_17485
PAESSCV20265_0747 SCV20265_3492
PAEUBN889_07250(phzE1_9)
PAEGAI22_00160 AI22_17565
PAECM802_1961 M802_4344
PAEOM801_1962 M801_4212
PCOFPOR16_00740
PFBVO64_0338
POIBOP93_12300
PCHEY04_26290
PCPJM49_05455
PSETTHL1_3622
PYMAK972_2557
PSEPC4K39_3097
BUUWS70_27185
BURBcep18194_B1570
BGLbglu_2g09260
BGUKS03_4991
BVRBVIR_2862
SECHB18_04215 B18_21405
MABMAB_0298
MMVMYCMA_0160
MABBMASS_0299
MABLMMASJCM_0295
MCHEBB28_01470
MIZBAB75_02020
MSTEMSTE_00260
MSAOMYCSP_01300
MSALDSM43276_00253(trpE_1)
CMVCMUST_14530
NBRO3I_033275
NTPCRH09_09655
NAHF5544_33405
SCOSCO2117(SC6E10.11)
SALBXNR_0598 XNR_4762
SMASAVERM_6084(phzE)
SGRSGR_5385
SGBWQO_08390 WQO_33795 WQO_33800
SCBSCAB_12051 SCAB_67631(trpE)
SSXSACTE_1549 SACTE_1751
SVLStrvi_7202
SHYSHJG_3601
SHOSHJGH_3366
SRCM271_35750
SMALSMALA_2052
SCTSCAT_1246(phzB)
SCYSCATT_12460
SFASfla_4700
SBHSBI_07844
SVESVEN_1778
SDVBN159_6319(phzB)
SALSSLNWT_4920 SLNWT_5765
STRPF750_1981
SFISFUL_1692 SFUL_3323 SFUL_5261
SCIB446_11110
SALLSAZ_14330
SLVSLIV_27120(phzB)
SGUSGLAU_09650 SGLAU_17225(phzB)
SVTSVTN_10295
STREGZL_06398
SCWTU94_09180
SLDT261_6049
SLCSL103_08680
SXISXIM_10580
STRMM444_10845 M444_18415
STRCAA958_06685 AA958_30775
SAMBSAM23877_2213(phzB)
SPRISPRI_5382
SCZABE83_19215 ABE83_26420 ABE83_27390
SCXAS200_32305
SRWTUE45_02649(trpE_2)
STRFASR50_10340
SLEsle_50210(sle_50210) sle_57420(sle_57420)
SRNA4G23_01307(trpG) A4G23_05416(trpE_2)
SPAVSpa2297_08695
STRTA8713_08010 A8713_24030
SCLFBB341_21450
SGSAVL59_37600
STSIA4E84_10935
SKYD0C37_25690
SALWCP975_09305
SGMGCM10017557_24340(phzE1) GCM10017557_60800
SCADDN051_27795 DN051_33895
SLSSLINC_2449 SLINC_5461
SNRSNOUR_29770
SPLULK06_007460 LK06_026285
STRDNI25_24445 NI25_28590
SNWBBN63_05565 BBN63_25995
SAUOBV401_13735
SSIAA7J05_26290
SVUB1H20_07495
SPUNBFF78_15755 BFF78_30585
SGVB1H19_12510
SLAUSLA_1712
SALFSMD44_02324(phzE)
SALJSMD11_1728 SMD11_3252 SMD11_4937(phzE)
SLXSLAV_26615(phnA)
STROSTRMOE7_11390
SFKKY5_2100
SGEDWG14_06212(trpE_1)
SRJSRO_5342
SLKSLUN_10395
SDXC4B68_29570
SGDELQ87_28725
SQZFQU76_00260 FQU76_07185 FQU76_33480
SCYAEJ357_12420
SASTCD934_07150 CD934_24910
SNQCP978_10230
STIRDDW44_03905 DDW44_31310 DDW44_31315
SKACP970_32080
SGZC0216_00430 C0216_23630
SVNCP980_14885 CP980_24250
SNKCP967_25455
SHAWCEB94_11480 CEB94_18880
SRKFGW37_08910 FGW37_24785
SFICEIZ62_24520
SGALCP966_09435
SSPODDQ41_03275
SVRCP971_17415 CP971_24495
SPADDVK44_06530 DVK44_32310
SFYGFH48_29340
SAQUEJC51_01045 EJC51_14230
SGFHEP81_05649
SCAVCVT27_07280 CVT27_15335
SSEOD0Z67_01710 D0Z67_07430
SBYH7H31_26910
SRIMCP984_30290
SSUBCP968_24085
SPHVF9278_10925
SCINCP977_09525 CP977_15860
SFUGCNQ36_09440
SPACB1H29_26765
STSUB7R87_07175
SCHACP983_31690
SGXH4W23_10720
SCOECP976_12245
SBATG4Z16_26055
SSPBCP982_12420 CP982_28275
SNFJYK04_02801(trpE_2)
SPLACP981_10505
SBROGQF42_13570
STUIGCM10017668_17660
SATAC5746_11605
SCHFIPT68_09945
SHUNDWB77_04282(phnA) DWB77_05612(trpE_1)
SHARHUT13_06035
SCYGS1361_11655(trpE2)
SFEUIM697_11280
SLIAHA039_25480 HA039_29905
SDWK7C20_10060
SVDCP969_11695
SCALI6J39_07700
SAUHSU9_008485
SFBCP974_07175 CP974_28835
SMOBJ7W19_08350
SPRACP972_09725
SPEUCGZ69_09165
SROIIAG44_13940 IAG44_29595 IAG44_38340
SXNIAG42_26030
SGJIAG43_07985
SHKJ2N69_09115
SANUK7396_26350
STRYEQG64_07380 EQG64_16155
SDDD9753_26095
SACTDMT42_10600
SMAOCAG99_00485 CAG99_15055 CAG99_22525
SGOBtest1122_03660
SINEKI385_13220
SLFJEQ17_03400 JEQ17_33870
KSKKSE_21580 KSE_58290 KSE_59080
KABB7C62_07380
KAUB6264_19060
KITCFP65_1906 CFP65_3610
STRIC7M71_006375 C7M71_014735
YIALO772_24360
MTSMTES_0388
MIMAKG07_02285
MIOAOA12_15035
MIXAB663_002144
MIPAXH82_13275
MCWA8L33_08180
MPALBO218_15790
MIHBJP65_03865
MICRBMW26_06855
MAURBOH66_12155
MHOSCXR34_12920
MFOLDXT68_06730
MOOBWL13_02068(trpE)
MLVCVS47_00972(trpE)
MWAE4K62_05860
MPRTET475_00770
MSEDE3O41_04100
MOYCVS54_02214(trpE)
MSFIT882_04630
MCAWF6J84_10575
MLZF6J85_05585
MCHNHCR76_06035
MRNK8F61_07210
MICSC1N74_11315
RTXTI83_09780
RTCAPU90_07285
RTNA6122_2322
RRYC1O28_10550
RIAC7V51_15390
RFSC1I64_12250
RTEGSU10_03085
AGYATC03_07245
AGMDCE93_03990
AGFET445_09520
HUWFPZ11_06840
GRYD7I44_05760
PLAPEAO79_11405
KSEKsed_06140
SANWG7063_02290
CEZCBP52_12010
SERJSGUI_2558
SERWFY030_13650
ORNDV701_08890
ORZFNH13_03665
TFABW733_09555
NCANoca_4529
NDKI601_1070(phnA_1)
NOYEXE57_12930
NOIFCL41_00890
NOOFE634_20225
NDPE2C04_01520
NSNEXE58_09250
NBEBack2_19310
NANOG5V58_23605
NMESH9L09_08155
NAROCFH99_01700
NAQUENKNEFLB_04326(phnA_2)
NROK8W59_00900
NOQLN652_11950
NCQK6T13_16420
NPCKUV85_12570
PSIMKR76_01360
AERAERYTH_00460
AEZC3E78_17405
AEBC6I20_16275
AEFGEV26_17160
MUZH4N58_18775
THAONI17_009040
TALXFOF52_14765
NDANdas_5031
STRREKD16_18445(phnA)
NOABKM31_19745
FREFranean1_3620 Franean1_3878
FALFRAAL3440
KRAKrad_1199
SACGFDZ84_16250
AMYCCU254_36715
APRECNX65_15960
SSYIEKG83_21245
KALKALB_2053
KPHYAOZ06_16445 AOZ06_18055 AOZ06_22500 AOZ06_25090
ALOCRK58741
STPStrop_2960
SAQSare_3182
MAUMicau_4172
MILML5_4130
MICBMicB006_2906 MicB006_3169
MTUACSH63_03790
MICHFJK98_01245 FJK98_22465
MTEMGCE86_15205
MCABHXZ27_21235
MSAGGCM10017556_03700(phzE1) GCM10017556_11920(phzE1)
VMAVAB18032_25130
ASEACPL_1962(phzE)
AMSAMIS_17120
ACTNL083_2374 L083_5379(phzE)
AFSAFR_11460
ACTSACWT_1840
AIHAiant_63010(phzE1)
PLKCIK06_19030 CIK06_19035
PLABC6361_09300
PLATC6W10_05810
PFLAPflav_059820(phzE1)
PSUUPsuf_006100(phzE1_1) Psuf_020570(phzE1_2) Psuf_091170(phzE1_3)
VERHUT12_21420
PRYPrubr_00870(phzE1_1) Prubr_64690(phzE1_2)
CATICS0771_71150(phzE1)
DVCDvina_11065 Dvina_40335
CCAZCOUCH_28315
CAICaci_0239
GLYK3N28_00675
ACTCCHIBA101_0088
AEYCDG81_16845
 » show all
Reference
1  [PMID:18182490]
  Authors
Van Lanen SG, Lin S, Shen B
  Title
Biosynthesis of the enediyne antitumor antibiotic C-1027 involves a new branching point in chorismate metabolism.
  Journal
Proc Natl Acad Sci U S A 105:494-9 (2008)
DOI:10.1073/pnas.0708750105
  Sequence
Reference
2
  Authors
Yu, L., Mah, S., Otani, T. and Dedon, P.
  Title
The benzoxazolinate of C-1027 confers intercalative DNA binding.
  Journal
J Am Chem Soc 117:8877-8878 (1995)
Reference
3  [PMID:11562236]
  Authors
McDonald M, Mavrodi DV, Thomashow LS, Floss HG
  Title
Phenazine biosynthesis in Pseudomonas fluorescens: branchpoint from the primary shikimate biosynthetic pathway and role of phenazine-1,6-dicarboxylic acid.
  Journal
J Am Chem Soc 123:9459-60 (2001)
DOI:10.1021/ja011243+
  Sequence
Reference
4  [PMID:15008629]
  Authors
Laursen JB, Nielsen J.
  Title
Phenazine natural products: biosynthesis, synthetic analogues, and biological activity.
  Journal
Chem Rev 104:1663-86 (2004)
DOI:10.1021/cr020473j
Other DBs
ExplorEnz - The Enzyme Database: 2.6.1.86
IUBMB Enzyme Nomenclature: 2.6.1.86
ExPASy - ENZYME nomenclature database: 2.6.1.86
BRENDA, the Enzyme Database: 2.6.1.86

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