KEGG   ENZYME: 2.7.7.100
Entry
EC 2.7.7.100                Enzyme                                 
Name
SAMP-activating enzyme;
UbaA (ambiguous);
SAMP-activating enzyme E1 (ambiguous)
Class
Transferases;
Transferring phosphorus-containing groups;
Nucleotidyltransferases
Sysname
ATP:[SAMP]-Gly-Gly adenylyltransferase
Reaction(IUBMB)
ATP + [SAMP]-Gly-Gly = diphosphate + [SAMP]-Gly-Gly-AMP [RN:R12099]
Reaction(KEGG)
R12099
Substrate
ATP [CPD:C00002];
[SAMP]-Gly-Gly [CPD:C21900]
Product
diphosphate [CPD:C00013];
[SAMP]-Gly-Gly-AMP [CPD:C21901]
Comment
Contains Zn2+. The enzyme catalyses the activation of SAMPs (Small Archaeal Modifier Proteins), which are ubiquitin-like proteins found only in the Archaea, by catalysing the ATP-dependent formation of a SAMP adenylate in which the C-terminal glycine of SAMP is bound to AMP via an acyl-phosphate linkage. The product of this activity can accept a sulfur atom to form a thiocarboxylate moiety that acts as a sulfur carrier involved in thiolation of tRNA and other metabolites such as molybdopterin. Alternatively, the enzyme can also catalyse the transfer of SAMP from its activated form to an internal cysteine residue, leading to a process termed SAMPylation (see EC 6.2.1.55, E1 SAMP-activating enzyme).
History
EC 2.7.7.100 created 2018
Orthology
K22837  SAMP-activating enzyme
Genes
CCROCMC5_032930
PAUUE8A73_024155
CTHMCFE_0903
PROSCHL67_09830
AFGAFULGI_00005400
APOArcpr_0731
AVEArcve_1268
ASTAsulf_01031
MBUMbur_0130(moeB)
MMETMCMEM_0684
MELOJ7W08_01410(moeB)
MMHMmah_0709
MZIHWN40_06855(moeB)
HALVNG_0084G(moeB)
HSLOE_1140R(ubaA)
HDLHALDL1_04665
HHBHhub_1334(ubaA)
HAGSJT689_01790(moeB)
HABOJRZ79_00280(moeB)
HNOLT974_01580(moeB)
HLULT972_12105(moeB)
HANRLJ422_00400(moeB)
HJEHacjB3_13610
HALHHTSR_0272
HHSRHSR6_0258(moeB)
HSUHLASF_0452
HSFHLASA_0450
SALRFQU85_02825(moeB)
HALREFA46_005410(moeB)
HARAAArcS_3002(thiF)
HAADMW046_10260(moeB)
HALXM0R89_09695(moeB)
HMArrnAC2090(moeB)
HHIHAH_2583(moeB)
HHNHISP_13140
HABSG26_05415
HTABVU17_12495
HALJG9465_06130(moeB)
HSINKDQ40_00450(moeB)
NPHNP_0052A(ubaA)
NMONmlp_1065(ubaA)
NHOHWV23_07470(moeB)
NSALHWV07_10275(moeB)
HUTHuta_0150
HTIHTIA_0141
HALAHrd1104_11100(moeB)
HMUHmuk_0279
HALZE5139_04685(moeB)
HALLLC1Hm_2808(ubaA)
HAZPGBQ70_04680(moeB)
HALIBV210_06270
HSNDV733_02735
HDSHSR122_2365(thiF)
HRRHZS55_04935(moeB)
HPELHZS54_05010(moeB)
HLTI7X12_08145(moeB)
HARCHARCEL1_07380
HDOMUK72_03880(moeB)
HWAHQ_1425A(ubaA)
HWCHqrw_1502(ubaA)
HVOHVO_0558(ubaA)
HMEHFX_0529(thiF)
HGIABY42_02550
HALEG3A49_02830(moeB)
HBOHbor_26540
HAQDU484_06075
HAJDU500_06515
HAERDU502_04115(moeB)
HRAEI982_09525(moeB)
HLMDV707_13865
HALMFCF25_09510(moeB)
HLAHlac_0421 Hlac_1265
HALPDOS48_02040
HALBEKH57_09530(moeB)
HEZZEO776_02865(moeB)
HALQHrr1229_004440(moeB) Hrr1229_015430(moeB)
HSSJ7656_13900(moeB)
HSAIHPS36_04750(moeB) HPS36_15130(moeB)
HAZZKI388_00500(moeB) KI388_12720(moeB)
SRUBC2R22_02680
HAEhalTADL_1694
HALNB4589_010465 B4589_016105(moeB)
HALGHUG10_17095(moeB)
HALUHUG12_18905(moeB) HUG12_19325(moeB)
HMPK6T50_11800
HREK6T36_11975
HRMK6T25_04600(moeB)
HACBHbl1158_03845(moeB)
HDFAArcSl_3027(moeB)
HAHHalar_2897
HTUHtur_3268
HDABB347_00300
HJTDVR14_11280
HSALJMJ58_12110(moeB)
HLOJ0X27_06555(moeB)
HAKZJ0X25_04400(moeB)
NMGNmag_1394(ubaA1)
HXAHalxa_1982
NATNJ7G_2757
NPENatpe_0399
NVRFEJ81_02060(moeB)
NPLFGF80_08945(moeB)
NAYHYG81_08715(moeB)
HALYHYG82_11695(moeB)
NGENatgr_1185
HRUHalru_0276
NOUNatoc_2086
SALIL593_07740
SAIMK0C01_07460(moeB)
HLRHALLA_16470
HLCCHINAEXTREME06765 CHINAEXTREME10020
NAJB1756_10410
NAGAArcMg_0423
NANAArc1_0432
NBGDV706_02105 DV706_03840
NASGCU68_09020(moeB)
 » show all
Reference
1  [PMID:21368171]
  Authors
Miranda HV, Nembhard N, Su D, Hepowit N, Krause DJ, Pritz JR, Phillips C, Soll D, Maupin-Furlow JA
  Title
E1- and ubiquitin-like proteins provide a direct link between protein conjugation and sulfur transfer in archaea.
  Journal
Proc Natl Acad Sci U S A 108:4417-22 (2011)
DOI:10.1073/pnas.1018151108
  Sequence
[hvo:HVO_0558]
Reference
2  [PMID:23140889]
  Authors
Maupin-Furlow JA
  Title
Ubiquitin-like proteins and their roles in archaea.
  Journal
Trends Microbiol 21:31-8 (2013)
DOI:10.1016/j.tim.2012.09.006
Reference
3  [PMID:27459543]
  Authors
Hepowit NL, de Vera IM, Cao S, Fu X, Wu Y, Uthandi S, Chavarria NE, Englert M, Su D, Sll D, Kojetin DJ, Maupin-Furlow JA
  Title
Mechanistic insight into protein modification and sulfur mobilization activities  of noncanonical E1 and associated ubiquitin-like proteins of Archaea.
  Journal
FEBS J 283:3567-3586 (2016)
DOI:10.1111/febs.13819
  Sequence
[hvo:HVO_0558]
Other DBs
ExplorEnz - The Enzyme Database: 2.7.7.100
IUBMB Enzyme Nomenclature: 2.7.7.100
ExPASy - ENZYME nomenclature database: 2.7.7.100
BRENDA, the Enzyme Database: 2.7.7.100

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