KEGG ENZYME: 2.7.7.73

ENZYME: 2.7.7.73

Entry

EC 2.7.7.73                 Enzyme

Name

sulfur carrier protein ThiS adenylyltransferase;
thiF (gene name)

Class

Transferases;
Transferring phosphorus-containing groups;
Nucleotidyltransferases

Sysname

ATP:[ThiS] adenylyltransferase

Reaction(IUBMB)

ATP + [ThiS] = diphosphate + adenylyl-[ThiS] [RN:R07459]

Reaction(KEGG)

R07459

Substrate

ATP [CPD:C00002];
[ThiS]

Product

diphosphate [CPD:C00013];
adenylyl-[ThiS]

Comment

Binds Zn2+. The enzyme catalyses the adenylation of ThiS, a sulfur carrier protein involved in the biosynthesis of thiamine.
The enzyme shows significant structural similarity to ubiquitin-activating enzyme [3,4]. In Escherichia coli, but not in Bacillus subtilis, the enzyme forms a cross link from Cys-184 to the ThiS carboxy terminus (the position that is also thiolated) via an acyldisulfide [2].

History

EC 2.7.7.73 created 2011

Pathway

ec00730

Thiamine metabolism

ec01100

Metabolic pathways

Orthology

K03148

sulfur carrier protein ThiS adenylyltransferase

Genes

ECO:	b3992(thiF)

ECJ:	JW3956(thiF)

ECD:	ECDH10B_4181(thiF)

EBW:	BWG_3652(thiF)

ECOK:

ECMDS42_3430(thiF)

ECE:	Z5567(thiF)

ECS:	ECs_4915(thiF)

ECF:	ECH74115_5460(thiF)

ETW:	ECSP_5062(thiF)

ELX:	CDCO157_4656(thiF)

EOI:	ECO111_4809(thiF)

EOJ:	ECO26_5101(thiF)

EOH:	ECO103_4741(thiF)

ECOO:

ECRM13514_5113(thiF)

ECOH:

ECRM13516_4849(thiF)

ESL:

O3K_23895

ESO:

O3O_01450

ESM:

O3M_23815

ECK:	EC55989_4477(thiF)

ECG:	E2348C_4299(thiF)

EOK:	G2583_4810(thiF)

ELR:	ECO55CA74_23020

ELH:

ETEC_4252

ECW:	EcE24377A_4534(thiF)

EUN:	UMNK88_4833(thiF)

ECP:

ECP_4205

ECOS:

EC958_5103(thiF)

ECV:	APECO1_2482(thiF)

ECOA:

APECO78_00725

ECX:	EcHS_A4226(thiF)

ECM:	EcSMS35_4440(thiF)

ECY:

ECSE_4280

ECR:	ECIAI1_4207(thiF)

ECQ:	ECED1_4699(thiF)

EUM:	ECUMN_4516(thiF)

ECT:	ECIAI39_4382(thiF)

EOC:	CE10_4672(thiF)

EBR:	ECB_03869(thiF)

EBL:	ECD_03869(thiF)

EBE:	B21_03822(thiF)

EBD:

ECBD_4040

ECI:	UTI89_C3827(thiF)

EIH:	ECOK1_4468(thiF)

ECZ:	ECS88_4453(thiF)

ECC:	c4949(thiF)

ELO:	EC042_4359(thiF)

ELN:	NRG857_19920

ESE:

ECSF_3846

ECL:	EcolC_4033

EKO:	EKO11_4329

EKF:	KO11_02410(thiF)

EAB:	ECABU_c45080(thiF)

EDH:	EcDH1_4002

EDJ:	ECDH1ME8569_3852(thiF)

ELU:	UM146_16705

ELW:	ECW_m4350(thiF)

ELL:	WFL_21175(thiF)

ELC:	i14_4539(thiF)

ELD:	i02_4539(thiF)

ELP:	P12B_c4104(thiF)

ELF:	LF82_2249(thiF)

ECOL:

LY180_20935

ECOI:

ECOPMV1_02797(thiF)

ECOJ:

P423_22130

EFE:	EFER_3762(thiF)

EAL:	EAKF1_ch1924(thiF)

EMA:	C1192_18240(thiF)

ESZ:	FEM44_11695(thiF)

STY:	STY3723(thiF)

STT:	t3469(thiF)

SEX:	STBHUCCB_36480

SENT:

TY21A_17535

STM:	STM4162(thiF)

SEO:	STM14_5003(thiF)

SEV:	STMMW_41151

SEY:	SL1344_4101(thiF)

SEM:	STMDT12_C42990

SEJ:	STMUK_4146(thiF)

SEB:	STM474_4348(thiF)

SEF:	UMN798_4509(thiF)

SETU:

STU288_20915

SETC:

CFSAN001921_19620

SENR:

STMDT2_40151(thiF)

SEND:

DT104_41591(thiF)

SENI:

CY43_21730

SEEN:

SE451236_00090

SPT:	SPA3999(thiF)

SEK:

SSPA3714

SPQ:	SPAB_05152

SEI:	SPC_3993(thiF)

SEC:	SCH_4043(thiF)

SEH:	SeHA_C4492

SHB:

SU5_0241

SENH:

CFSAN002069_11305

SEEH:

SEEH1578_06990

SEE:	SNSL254_A4494(thiF)

SENN:

SN31241_45350

SEW:	SeSA_A4372

SEA:	SeAg_B4405

SENS:

Q786_20385

SED:	SeD_A4568(thiF)

SEG:	SG3444(thiF)

SEL:	SPUL_3422(thiF)

SEGA:

SPUCDC_3408(thiF)

SET:	SEN3948(thiF)

SENA:

AU38_20430

SENO:

AU37_20445

SENV:

AU39_20465

SENQ:

AU40_22840

SENL:

IY59_20935

SENJ:

CFSAN001992_12950

SEEC:

CFSAN002050_03625

SEEB:

SEEB0189_021550

SEEP:

I137_16355

SENB:

BN855_42280(thiG)

SENE:

IA1_20240

SENC:

SEET0819_04605

SES:	SARI_03496

SBG:	SBG_3636(thiF)

SBZ:

A464_4174

SBV:	N643_18330

SALZ:

EOS98_21520(thiF)

SFL:	SF4064(thiF)

SFX:	S3671(thiF)

SFV:	SFV_4064(thiF)

SFE:	SFxv_4431(thiF)

SFN:

SFy_5821

SFS:

SFyv_5888

SFT:	NCTC1_04410(thiF_2)

SSN:	SSON_4165(thiF)

SDY:	SDY_3734(thiF)

SDZ:	Asd1617_04925

SHQ:	A0259_01550

ENC:

ECL_00253

ENL:	A3UG_01270

ECLG:

EC036_02420

ECLE:

ECNIH2_01915

ECLN:

ECNIH4_01240

ECLI:

ECNIH5_01465

ECLX:

LI66_01310

ECLY:

LI62_01720

ECLZ:

LI64_01445

ECLO:

ENC_01310

EHM:	AB284_24095

EXF:	BFV63_01290

ECLA:

ECNIH3_01455

ECLC:

ECR091_01455

EAU:	DI57_17435

EKB:	BFV64_00985

ENO:	ECENHK_01205

EEC:	EcWSU1_00219(thiF)

ELG:	BH714_16265

ECAN:

CWI88_01220

ERN:	BFV67_01050

ECLS:

LI67_002025

ECHG:

FY206_01185(thiF)

ESH:	C1N69_01115

ENT:	Ent638_0205

EAS:	Entas_0227

ENR:	H650_16545

ENX:	NI40_001010

ENF:	AKI40_4716

LNI:	CWR52_11430

EBG:	FAI37_05495

END:	A4308_08220

ENZ:	G0034_01010(thiF)

ENS:	HWQ15_09620(thiF)

ENK:	LOC22_12130(thiF)

EHU:	D5067_0022060

ESA:

ESA_03681

CSK:	ES15_3621(thiF)

CSZ:	CSSP291_17070

CSJ:	CSK29544_00651

CCON:

AFK62_01730

CDM:	AFK67_01695

CSI:	P262_05389

CMJ:	AFK66_018395

CUI:	AFK65_01640

CMW:	AFK63_16915

CTU:	CTU_03030(thiF)

KPN:	KPN_04374(thiF)

KPU:	KP1_0232(thiF)

KPM:	KPHS_02110

KPP:

A79E_4813

KPH:	KPNIH24_01155

KPZ:	KPNIH27_01180

KPV:	KPNIH29_01145

KPW:	KPNIH30_01180

KPY:	KPNIH31_01175

KPG:	KPNIH32_01185

KPC:	KPNIH10_01150

KPQ:	KPR0928_01155

KPT:	VK055_3100(thiF)

KPO:	KPN2242_24820

KPR:	KPR_0312(thiF)

KPJ:

N559_4923

KPI:	D364_22275

KPA:	KPNJ1_05223

KPS:	KPNJ2_00433

KPX:	PMK1_01857(thiF_2)

KPB:	FH42_18230

KPNE:

KU54_025550

KPNU:

LI86_25390

KPNK:

BN49_4466(thiF)

KVA:

Kvar_4868

KPE:	KPK_5300(thiF)

KPK:	A593_11960

KVD:	KR75_09400

KVQ:	SP68_15990

KOX:

KOX_07955

KOE:

A225_0250

KOY:	J415_01790

KOM:	HR38_06605

KMI:	VW41_18145

KOK:	KONIH1_01470

KOC:	AB185_05745

KQU:	AVR78_05730

EAE:

EAE_08170

EAR:

CCG32365

KQV:	B8P98_26355

KLL:	BJF97_01320

KLW:	DA718_27830

KAR:	LGL98_23800

KGR:	JJJ10_26295

ROR:	RORB6_17555

RON:	TE10_03490

RPLN:

B1209_25665

RAO:	DSD31_24495

RTG:	NCTC13098_06934(thiF_2)

REE:	electrica_04813(thiF_2)

CRO:	ROD_37601(thiF)

CKO:

CKO_02994

CFD:	CFNIH1_07565

CBRA:

A6J81_15725

CWE:	CO701_20725

CYO:	CD187_22695

CPOT:

FOB25_09975

CFQ:	C2U38_24480

CSED:

JY391_21100

CAMA:

F384_21975

CAF:	AL524_04750

CIF:	AL515_02610

CFAR:

CI104_23725

CIR:	C2U53_11330

CIE:	AN232_02970

CPAR:

CUC49_20605

CTEL:

GBC03_06190(moeB)

CITZ:

E4Z61_15730(thiF)

CARS:

E1B03_02395

EBT:	EBL_c37150(thiF)

CNT:	JT31_11260

CEM:	LH23_06450

CEN:	LH86_06380

CLAP:

NCTC11466_04347(thiF_2)

PGE:	LG71_01830

ESC:	Entcl_4167

KLE:	AO703_01100

KSA:	C813_14120

KOR:	AWR26_25350

KRD:	A3780_25975

KCO:	BWI95_00705

KOT:	EH164_21075

KPSE:

IP581_01175

KGO:	CEW81_23455

KIE:	NCTC12125_03512(thiF_1)

KAS:	KATP_42780

LAX:	APT61_21160

LEI:	C2U54_05960

LEH:	C3F35_10615

LEE:	DVA44_01005(thiF)

LER:	GNG29_00970(moeB)

LEA:	GNG26_00995(moeB)

LPNU:

KQ929_19520

LAZ:	A8A57_01235

LEF:	LJPFL01_0205

LEW:	DAI21_03275(thiF)

BUF:	D8682_14255

BAGE:

BADSM9389_39210

BFT:	MNO13_23085

SYM:	K6K13_22475

METY:

MRY16398_53680

YRE:	HEC60_19110

SGOE:

A8O29_021330

KIN:	AB182_23775

PDZ:	HHA33_00780

IZH:	FEM41_07025

PSHI:

SAMEA2665130_3035(thiF)

PSGC:

G163CM_33230(thiF)

EBF:

D782_4277

EBC:	C2U52_06705(thiF)

EBU:	CUC76_14225(thiF)

YPE:	YPO3741(thiF)

YPK:	y0489(thiF)

YPA:

YPA_3613

YPN:

YPN_0225

YPM:	YP_3104(thiF)

YPP:	YPDSF_3738

YPG:	YpAngola_A0453(thiF)

YPZ:	YPZ3_3301(thiF)

YPT:	A1122_07155

YPD:	YPD4_3293(thiF)

YPX:	YPD8_3293(thiF)

YPW:

CH59_1664

YPJ:

CH55_3286

YPV:

BZ15_3985

YPL:

CH46_1336

YPS:	YPTB0288(thiF)

YPO:

BZ17_2285

YPI:	YpsIP31758_3855(thiF)

YPY:

YPK_0345

YPB:

YPTS_0309

YPQ:

DJ40_2123

YPU:

BZ21_3655

YPR:

BZ20_1826

YPC:

BZ23_3927

YPF:

BZ19_3758

YEN:	YE0291(thiF)

YEP:	YE105_C0293

YEY:

Y11_35001

YEL:	LC20_04937

YEW:

CH47_3185

YET:

CH48_2000

YEF:	FORC2_3814

YEE:	YE5303_43491(thiF)

YSI:	BF17_09535

YAL:

AT01_2173

YFR:	AW19_2904(thiF)

YIN:

CH53_2100

YKR:

CH54_2885

YRO:

CH64_2889

YRU:

BD65_1644

YRB:	UGYR_08750

YAK:	ACZ76_07440

YMA:	DA391_21355

YHI:	D5F51_01575

YCA:	F0T03_20100

YMO:	HRD69_03670

SMAR:

SM39_4219(thiF)

SMAC:

SMDB11_4386(thiF)

SMW:	SMWW4_v1c02880(thiF)

SPE:

Spro_0282

SRR:	SerAS9_0244

SRL:	SOD_c02430(thiF)

SRY:	M621_01235

SPLY:

Q5A_001345(thiF)

SRS:	SerAS12_0244

SRA:	SerAS13_0243

SMAF:

D781_0260

SLQ:	M495_25105

SERF:

L085_05825

SERS:

SERRSCBI_01300

SFW:	WN53_09940

SFG:	AV650_01055

SRZ:	AXX16_0258

SERA:

Ser39006_002310

SERQ:

CWC46_02310

SERM:

CLM71_01420

SQU:	E4343_14795

SFJ:	SAMEA4384070_0271(thiF)

SOF:	NCTC11214_02089(thiF)

SSUR:

ATE40_022185

SURI:

J0X03_22570

SRHZ:

FO014_08560

SENP:

KHA73_21870

SFO:	Z042_13540

RAH:	Rahaq_4128

RACE:

JHW33_14990

RAQ:	Rahaq2_4237

RAA:

Q7S_20975

ROX:	BV494_18380

RVC:	J9880_06125

GQU:	AWC35_10060

EAME:

GXP68_19760

RBAD:

H2866_09465

ECA:	ECA0230(thiF)

PATR:

EV46_01250

PATO:

GZ59_02540(thiF)

PCT:

PC1_0214

PCC:	PCC21_002200

PCV:	BCS7_01005

PWA:	Pecwa_0218

PPAR:

A8F97_17285

PEC:

W5S_0229

PWS:	A7983_06380

PPOA:

BJK05_17730

PBRA:

B5S52_20710

PPUJ:

E2566_01305

PARI:

I2D83_20790

PAQU:

DMB82_0001035(moeB)

PVZ:	OA04_02590(thiF)

PPAV:

LOZ86_02025

DDD:	Dda3937_00233(thiF)

DZE:	Dd1591_3872

DDC:	Dd586_0229

DZC:	W909_01170

DSO:	A4U42_10335

CED:	LH89_11005

DFN:	CVE23_01260

DDQ:

DDI_4556

DAQ:	DAQ1742_00303(thiF)

DIC:	Dpoa569_000211

DDA:	Dd703_3717

BGJ:	AWC36_17175

BRB:	EH207_16445

BNG:	EH206_21160

BIZ:	HC231_22345

LBQ:	CKQ53_06240

LPOP:

I6N93_15955

SOD:	Sant_3918(thiF)

EBI:	EbC_02650(thiF)

EHD:	ERCIPSTX3056_680(thiF)

ETP:	LU633_01440

PANP:

PSNIH2_19470

PALH:

B1H58_08975

PGZ:	C2E15_19920

PCD:	C2E16_19335

MINT:

C7M51_03123(thiF)

MTHI:

C7M52_03870(thiF)

TCI:	A7K98_18355

TPTY:

NCTC11468_00196(thiF)

PLU:	plu0484(thiF)

PLUM:

A4R40_02375

PAY:	PAU_00392(thiF)

PTT:	VY86_05715

PAKH:

B0X70_02300

PLUI:

CE143_02285

PMR:	PMI2779(thiF)

PMIB:

BB2000_2790(thiF)

PVL:	AOB99_15150

PVG:	CRN77_05695

PHAU:

PH4a_05475

PROT:

BTA34_15200

PCOL:

F1325_17415

PCIB:

F9282_18065

PPEE:

I6G31_11200

XBO:	XBJ1_4030(thiF)

XBV:	XBW1_0430(thiF)

XNE:	XNC1_0584(thiF)

XNM:	XNC2_0571(thiF)

XDO:	XDD1_3617(thiF)

XPO:	XPG1_3149(thiF)

XHO:	A9255_16780

XBU:	HGO23_01605

PSI:

S70_08570

PSX:

DR96_1464

PSTA:

BGK56_11615

PRG:	RB151_004040(thiF)

PALA:

CO695_02885

PHEI:

NCTC12003_00402(thiF)

PRQ:	CYG50_14125

PRJ:	NCTC6933_00458(thiF)

PVC:	G3341_01755

MMK:

MU9_699

AEN:	ARADI_0629(thiF)

ANS:	ArsFIN_41430(thiF)

MWI:	MNY66_01320

ETE:	ETEE_2783(thiF)

EDW:	QY76_15685

EDL:	AAZ33_04770

HAV:	AT03_02270

HPAR:

AL518_03325

OPO:	DSM2777_01720

LPV:	HYN51_11460

PFQ:	QQ39_15885

PRAG:

EKN56_17900

LRI:	NCTC12151_00409(thiF)

PATL:

KGI96_02630

APL:	APL_0533(thiF)

APJ:	APJL_0523(thiF)

APA:	APP7_0572(thiF)

AEU:	ACEE_04500

ALIG:

NCTC10568_01008(thiF)

OOI:	A6A10_00240

VCH:

VC_0063

VCF:	IR04_05255

VCS:

MS6_2554

VCE:	Vch1786_I2343(thiF)

VCQ:	EN18_17215

VCJ:	VCD_001531

VCI:

O3Y_00295

VCO:	VC0395_A2450(thiF)

VCR:	VC395_0117(thiF)

VCM:	VCM66_0063(thiF)

VCL:	VCLMA_A0059(thiF)

VCX:	VAA049_55(thiF)

VCZ:	VAB027_55(thiF)

VVU:

VV1_0962

VVY:

VV3205

VVM:	VVMO6_02940

VVL:	VV93_v1c29240(thiF)

VPA:

VP3025

VPB:

VPBB_2856

VPK:	M636_22140

VPF:	M634_01660

VPH:	VPUCM_3104

VHA:	VIBHAR_00364

VCA:	M892_10910

VAG:

N646_2120

VEX:	VEA_002074

VDB:	AL552_24185

VHR:	AL538_09335

VNA:	PN96_13670

VOW:	A9237_08515

VRO:	BSZ04_23660

VSP:

VS_3096

VEJ:	VEJY3_15240

VFU:	vfu_A00370

VAN:

VAA_00206

LAG:	N175_01275

VAU:	VANGNB10_cI2687c(thiF)

VCY:	IX92_00190

VCT:	JV59_39470

VTU:	IX91_15235

VEU:	IXK98_23945

VSI:	MTO69_13415

VFL:	AL536_20485

VMI:	AL543_09260

VBR:	A6E01_12910

VSC:	VSVS12_00161(thiF)

VGA:	BSQ33_08650

VSH:	BSZ05_05980

VQI:	CCZ37_13120

VTA:	A3230(thiF)

VAF:	D1115_00220(thiF)

VNL:	D3H41_16405

VCC:	FAZ90_00170

VAS:	GT360_14475

VAQ:	FIV01_14535(thiF)

VSR:	Vspart_03405(thiF)

VKA:	BTD91_06575

VZI:	G5S32_14685

VNV:	IF132_15540

VGI:	MID13_15295

VFI:	VF_0034(thiF)

VFM:	VFMJ11_0035

VSA:	VSAL_I2985(thiF)

AWD:	AWOD_I_0034(thiF)

PPR:	PBPRA0108(BP0610)

PGB:	H744_2c0352

PDS:	CAY62_13925

GHO:	AL542_09960

SALY:

E8E00_13495

SKS:	FCN78_00145

SCOT:

HBA18_00150

SON:	SO_2443(thiF)

SDN:

Sden_1772

SFR:

Sfri_2199

SAZ:

Sama_1858

SBL:

Sbal_2024

SBM:	Shew185_2321

SBN:	Sbal195_2437

SBP:	Sbal223_2026

SBT:	Sbal678_2438

SBS:	Sbal117_2232

SBB:	Sbal175_2087

SLO:

Shew_2094

SPC:	Sputcn32_1924

SHP:	Sput200_2066

SSE:

Ssed_2015

SPL:

Spea_2381

SHE:	Shewmr4_1883

SHM:	Shewmr7_2095

SHN:	Shewana3_1938

SHW:	Sputw3181_2084

SHL:

Shal_1902

SWD:

Swoo_2590

SWP:

swp_2332

SVO:

SVI_2369

SHF:	CEQ32_05065

SJA:	SJ2017_1844

SPSW:

Sps_01182

SBJ:	CF168_11010

SMAV:

CFF01_08810

SHEW:

CKQ84_20500

SLJ:	EGC82_10145

SMAI:

EXU30_20095

SPOL:

FH971_11060

SBK:	SHEWBE_2568(thiF)

SKH:	STH12_01548(thiF)

SLIT:

JQC75_09430

SPSH:

FM037_15555

SEUR:

FM038_010955

SCYP:

JYB88_08305

SSEM:

JYB85_08495

SGLA:

FJ709_08365

SINV:

K8B83_13645

SXM:	MKD32_09685

ILO:	IL0767(thiF)

ILI:	K734_03845

IPI:	CEW91_07265

IDI:	CWC33_00110

IDT:	C5610_10745

IAB:	K5X84_07080

CPS:	CPS_4642(thiF)

COM:	CMT41_16345

COZ:	A3Q34_07635

COLW:

A3Q33_11740

COLA:

DBO93_15285

COV:	EKO29_03995

CBER:

B5D82_11705

LSD:	EMK97_12980

AMC:	MADE_1000710

AMH:	I633_00790

AMAA:

amad1_00655

AMAL:

I607_00610

AMAE:

I876_00625

AMAO:

I634_00615

AMAD:

I636_00650

AMAI:

I635_00650

AMAG:

I533_00635

AMAC:

MASE_00460

AMB:	AMBAS45_00500

AMG:	AMEC673_00510

AMK:	AMBLS11_00485

ALT:	ambt_18455

AAL:	EP13_00530

AAUS:

EP12_00505

ASP:	AOR13_3378

ASQ:	AVL57_01895

AAW:	AVL56_00705

ALE:	AV939_00710

ALZ:	AV940_00710

APEL:

CA267_010245

GPS:

C427_5286

LAL:	AT746_14825

SALH:

HMF8227_02268(thiF)

AGZ:	M0C34_19530(moeB)

PIN:

Ping_0362

FBL:

Fbal_2032

MII:	BTJ40_16205

MICZ:

GL2_25850(thiF)

ZPL:	ZBT109_1918

AHA:	AHA_0441(thiF)

AHY:	AHML_02280

AHD:	AI20_17180

AHR:	V428_02420

AHP:	V429_02420

AHJ:	V469_20900

AHH:	RY45_02520

AHI:	VU14_20280

AAJ:	BOQ57_02125

ASA:	ASA_3903(thiF)

AEO:	O23A_p4100

AVR:

B565_3623

AVO:	AMS64_21150

AMED:

B224_6005

ASR:	WL1483_1377(thiF)

ADH:	CK627_05995

AEM:	CK911_19050

AEA:	C2U39_01395

ARV:	C7N77_02395

AES:	C2U30_16515

AEL:	NCTC12917_00354(thiF)

ASIM:

FE240_00775

AALL:

I6G90_17955

AJD:	I6H43_08460

ARIV:

KYK33_01735

TAU:

Tola_1076

FCE:	JN531_011410

BCI:	BCI_0043(thiF)

BCIB:

IM45_104

MANC:

IV454_27705

HMS:	HMU13210(moeB)

CJE:	Cj1046c(moeB)

CJB:	BN148_1046c(moeB)

CJJ:	CJJ81176_1067(thiF)

CJU:	C8J_0983(moeB)

CJN:	ICDCCJ07001_1009

CJI:	CJSA_0989(moeB)

CJM:	CJM1_1021(thiF)

CJS:	CJS3_1095(thiF)

CJP:	A911_05060

CJEJ:

N564_01013

CJEU:

N565_01059

CJEN:

N755_01052

CJEI:

N135_01082

CJER:

H730_06205

CJV:	MTVDSCj20_1048(thiF)

CJY:	QZ67_01119(moeZ)

CJQ:	UC78_1003(moeZ)

CJL:	PJ17_05375

CJW:	PJ18_05180

CJR:	CJE1190(thiF)

CJD:	JJD26997_0735(thiF)

CJZ:	M635_00690

CJX:	BN867_10350

CFF:	CFF8240_0074(thiF)

CFT:	CFF04554_0074(thiF)

CFV:	CFVI03293_0074(thiF)

CFX:	CFV97608_0074(thiF)

CFZ:

CSG_760

CAMP:

CFT03427_0079(thiF)

CFP:	CR44_00410

CCV:	CCV52592_1866(thiF)

CHA:	CHAB381_1178(thiF)

CCO:	CCC13826_1276(thiF)

CCOC:

CCON33237_0911(thiF)

CLA:	CLA_1148(thiF)

CLR:	UPTC16701_1122(thiF)

CLM:	UPTC16712_1153(thiF)

CLQ:	UPTC4110_1132(thiF)

CLN:	UPTC3659_1365(thiF)

CLL:	CONCH_1103(thiF)

CCOL:

BN865_10850

CCC:	G157_03745

CCQ:	N149_0981(thiF)

CCF:

YSQ_03790

CCY:

YSS_05655

CCOI:

YSU_03820

CCOF:

VC76_05090(moeZ)

CCOO:

ATE51_01528(moeZ)

CAJ:	CIG1485E_0484(thiF)

CIS:	CINS_1110(thiF)

CVO:	CVOL_1123(thiF)

CPEL:

CPEL_1257(thiF)

CAMR:

CAQ16704_1141(thiF)

CSM:	CSUB8521_1315(thiF)

CSF:	CSUB8523_1420(thiF)

CGRA:

CGRAC_1781(thiF)

CHYO:

CHH_1393(thiF)

CHV:	CHELV3228_1280(thiF)

CSPF:

CSF_0426(thiF)

CPIN:

CPIN18020_0736(thiF)

CCUN:

CCUN_1773(thiF)

CLX:	CLAN_0984(thiF)

CAVI:

CAV_1317(thiF)

CHW:	A2J15_001720(thiF)

CAMZ:

CVIC12175_0608(thiF)

CAMY:

CSUIS_1035(thiF)

COJ:	CORN_1220(thiFIII)

CUX:	CUP3940_0613(thiFIII)

CRX:	CRECT_0249(thiF)

CGEO:

CGEO_1455(thiF)

CBLA:

CBLAS_1323(thiF)

CCOR:

CCORG_0922(thiF)

CARM:

CARM_1168(thiFIII)

CMUC:

CMCT_0825(thiFIII)

CSHO:

CSHOW_0391(thiF)

CINF:

CINF_1614(thiFIII)

CNV:	CNZW441b_1030(thiFIII)

SBA:	Sulba_1545

APOC:

APORC_0154(thiFIII)

ATHR:

ATH_0149(thiFIII)

GEF:	FO488_13740(thiF)

GLO:

Glov_3072

GSB:	GSUB_11880

PCA:	Pcar_0611(thiF-2) Pcar_2513(thiF-1)

PACE:

A6070_02710 A6070_12660

PEF:	A7E78_08610

PPD:

Ppro_1833

DES:	DSOUD_2998(thiF)

DEU:

DBW_1048

DVE:	DESUT3_30170

DVU:	DVU_0643 DVU_2092

DVL:

Dvul_1138

DVM:

DvMF_0798

DVG:	Deval_1944

DDS:

Ddes_2168

DFI:	AXF13_14695

DPG:	DESPIGER_1676

DEF:	CNY67_01245

DFL:

DFE_1469

DDE:

Dde_1557

DSA:	Desal_2650

DHY:	DESAM_21862

DAS:

Daes_1269

DPI:

BN4_10828

DEJ:	AWY79_09940

PPRF:

DPRO_1176

PSEL:

GM415_11370(thiF)

DDN:	DND132_0865

LIP:	LI0506(thiF)

LIR:	LAW_00521(thiF)

PBIZ:

LWC08_12450 LWC08_13535(thiF)

DBA:

Dbac_0774

DOA:	AXF15_10050

DRT:

Dret_0729

DSF:

UWK_01895

DPR:	Despr_1927

DOG:	HP555_11265(thiF)

DBK:	DGMP_34630

DTO:	TOL2_C00580(thiF)

DWD:	DSCW_62770

DALK:

DSCA_59020

DEK:	DSLASN_00570 DSLASN_17020

SAT:

SYN_00438

AMA:	AM088(thiF)

AMF:	AMF_063(thiF)

ERU:	Erum8480(probable_thiF)

ERW:	ERWE_CDS_08990(thiF)

ERG:	ERGA_CDS_08890(thiF)

ECN:

Ecaj_0885

RJG:	CCGE525_37415

SDO:	SD1155_02270

BSU:	BSU11700(thiF)

BSR:

I33_1304

BSL:

A7A1_0987

BSH:	BSU6051_11700(thiF)

BSY:	I653_05945

BSUT:

BSUB_01272(thiF)

BSUL:

BSUA_01272(thiF)

BSUS:

Q433_06610

BSO:	BSNT_07613(thiF)

BSN:	BSn5_17765

BSQ:	B657_11700(thiF)

BSX:	C663_1198(thiF)

BSP:	U712_06020

BSS:	BSUW23_05915(thiF)

BST:

GYO_1475

BLI:	BL01587(thiF)

BLD:	BLi01264(thiF)

BLH:	BaLi_c13990(thiF)

BAY:	RBAM_011710

BAQ:	BACAU_1132(thiF)

BYA:	BANAU_1077(thiF)

BAMP:

B938_05695

BQY:	MUS_1215(thiF)

BAML:

BAM5036_1071(thiF)

BAMA:

RBAU_1133(thiF)

BAMN:

BASU_1110(thiF)

BAMB:

BAPNAU_2624(thiF)

BAMT:

AJ82_06580

BAMY:

V529_11190(thiF)

BMP:	NG74_01185(thiF_1)

BAO:	BAMF_1244(thiF1)

BAZ:	BAMTA208_11750(thiF)

BQL:	LL3_01251(thiF)

BXH:	BAXH7_02399(thiF)

BAMI:

KSO_013985

BAMC:

U471_11680

BAMF:

U722_05970

BSIA:

CWD84_15885

BAE:	BATR1942_03495

BVM:	B9C48_05885

BSON:

S101395_03585

BHT:	DIC78_03725

BMOJ:

HC660_12280(thiF)

BTEQ:

G4P54_06155

BIQ:	AN935_06075

BAN:

BA_0733

BAR:

GBAA_0733

BAT:

BAS0699

BAH:	BAMEG_3826

BAI:

BAA_0840

BAX:	H9401_0702

BANT:

A16_08180

BANR:

A16R_08260

BANS:

BAPAT_0709

BANH:

HYU01_04035

BANV:

DJ46_5213(thiF)

BCE:

BC0750

BCA:

BCE_0803

BCZ:	BCE33L0643(thiF)

BCR:	BCAH187_A0887

BCB:	BCB4264_A0790

BCU:	BCAH820_0810

BCG:	BCG9842_B4541

BCQ:	BCQ_0824(thiF)

BCX:

BCA_0796

BAL:	BACI_c07640(thiF)

BNC:

BCN_0711

BCF:

bcf_03865

BCER:

BCK_04470

BCEF:

BcrFT9_00682

BCY:	Bcer98_0620

BTK:	BT9727_0643(thiF)

BTL:	BALH_0670(thiF)

BTB:	BMB171_C0643

BTT:

HD73_0868

BTHR:

YBT1520_04300

BTHI:

BTK_04325

BTC:	CT43_CH0711

BTF:	YBT020_03945

BTM:

MC28_0013

BTG:	BTB_c08260(thiF1)

BTI:

BTG_17470

BTN:	BTF1_01380

BTHT:

H175_ch0717

BTHU:

YBT1518_04680

BTW:	BF38_2000(thiF)

BTHY:

AQ980_26550

BWE:	BcerKBAB4_0649

BWW:	bwei_4256(thiF)

BMYO:

BG05_5168

BTY:	Btoyo_3431

BMYC:

DJ92_3344

BBY:	CY96_02920

BWD:	CT694_04200(thiF)

BTRO:

FJR70_26075(thiF)

BMOB:

MLA2C4_03960

BNT:	GSN03_04090(thiF)

BPAC:

LMD38_22270(thiF)

BPU:

BPUM_1099

BPUM:

BW16_06235

BPUS:

UP12_05835

BJS:

MY9_1275

BACI:

B1NLA3E_07785

BMET:

BMMGA3_01115(moeB)

GST:	HW35_12430

BACW:

QR42_05670

BACP:

SB24_04015

BACB:

OY17_08555

BACY:

QF06_04800

BACL:

BS34A_12930(thiF_1)

BALM:

BsLM_1224

BGY:	BGLY_1283(thiF)

BWH:	A9C19_14755

BXI:	BK049_02400

BALT:

CFN77_05835

BACS:

AUL54_04775

BSAF:

BSL056_05775

BIT:	BIS30_16555

BACQ:

DOE78_13045(thiS)

BFD:	NCTC4823_00323(thiF_1) NCTC4823_00383(thiF_2)

BDA:	FSZ17_22260

BSHI:

LGQ02_16060

BBAD:

K7T73_07485 K7T73_08895

BCAB:

EFK13_06530(thiF)

BRY:	M0696_06445(thiF)

BCO:	Bcell_0764

BHK:	B4U37_06165

BCOH:

BC6307_03715

BCIR:

C2I06_18825

BMQ:	BMQ_0552(thiF)

BMD:	BMD_0555(thiF)

BMH:	BMWSH_4681

BMEG:

BG04_2844

BEO:

BEH_03355

BFX:	BC359_17765

BGI:	BGM20_00075 BGM20_21155

BON:	A361_02680

CGOT:

J1899_01415 J1899_02375 J1899_21250

CSUA:

IM538_04310

GKA:

GK0626

GTE:	GTCCBUS3UF5_6940

GTK:	GT3570_03015

GTM:	GT3921_16465

GLI:	GLN3_13340

GTN:	GTNG_0539(moeB)

GWC:	GWCH70_0614

GYC:	GYMC61_1418

GYA:	GYMC52_0539

GCT:	GC56T3_2935

GMC:	GY4MC1_3183

GGH:	GHH_c05790(thiF)

GJF:	M493_03265

GEA:	GARCT_00600(moeB)

GEL:	IB49_13405

GSE:	GT50_12645

GSR:	GS3922_12875

GZA:	IC807_16685

GTH:	Geoth_3203

PTL:	AOT13_06610

PTB:	DER53_07650

PCAL:

BV455_01132(moeB)

ANM:	GFC28_1931

AAMY:

GFC30_1003

ANL:	GFC29_3503

AND:	GRQ40_03595

ACAI:

ISX45_00450

LAGR:

FJQ98_02360

TAID:

KS242_01670

VIR:	X953_15605

VHL:	BME96_15090

VIL:	CFK37_18000

VIM:	GWK91_05225

VPT:	KBP50_13580

LAO:	AOX59_02220

FPN:	ABE65_019760

FAR:	ABE41_018765

SJE:	AAV35_010960

APAK:

AP3564_10230

BSJ:	UP17_10615

BMUR:

ABE28_010685

PBUT:

DTO10_11050 DTO10_24130

PASA:

BAOM_1975

PPSR:

I6J18_15320

PFRI:

L8956_11445 L8956_25930

AQT:	FN924_04425

GRC:	GI584_17345

SCIB:

HUG20_16605

SCIA:

HUG15_19525

NMK:	CHR53_16575 CHR53_27340

NTM:	BTDUT50_04350 BTDUT50_10955

MEKU:

HUW50_13750

MSEM:

GMB29_05050

MDG:	K8L98_06040

BLEN:

NCTC4824_01717(thiF)

SEDD:

ERJ70_03260

BVQ:	FHE72_12255

RMF:	D5E69_12075

BSE:

Bsel_2689

GAJ:	MY490_18580

PSUA:

FLK61_23705

HYI:	K2M58_07995

SER:

SERP2074

SEP:

SE_2061

SEPP:

SEB_02067

SEPS:

DP17_1003(thiF)

SHA:

SH0561

SHH:	ShL2_00456

SSP:

SSPP132

SCA:

SCA_2080

SLG:	SLGD_00494

SLN:	SLUG_04920

SSD:	SPSINT_0378

SDT:

SPSE_2076

SDP:	NCTC12225_02297(moeB_2)

SWA:	A284_01960

SPAS:

STP1_1001(thiF)

SXY:	BE24_11320

SXL:	SXYLSMQ121_0154

SXO:	SXYL_00147

SHU:	SHYC_10360

SCAP:

AYP1020_1668(moeB_2)

SSCH:

LH95_09875

SSCZ:

RN70_10640

SAGQ:

EP23_11785

SEQO:

SE1039_24250

SSIF:

AL483_11970

SCV:	A4G25_09815

SPET:

CEP67_07430

SLZ:	B5P37_07785

SKL:	C7J89_03125

SFQ:	C7J90_01370

SHOM:

EGX58_06350

SMUS:

C7J88_08795

SCAR:

DWB96_02280

SCHR:

DWB92_10515

SARL:

SAP2_03670

SPIC:

SAMEA4384060_0468(moeB_1)

SDB:	CNQ82_03135

SLLO:

ISP08_01940

SAUL:

I6G39_00680

SSCU:

CEP64_00935

SFF:	FOB90_09575

MLEN:

H3V22_07375

MVT:	I6J10_09715

MCL:

MCCL_0117

MCAK:

MCCS_02240(moeB_1)

MACR:

BHM04_09680

MBOE:

HT586_01250

MARY:

LAU42_00635

MBRU:

MGG13_00515

SHV:	AAT16_06180

SBAC:

BVH56_01210

JEA:	JEM45_07390

EAT:	EAT1b_1918

EACE:

KKI46_06355

BCOP:

JD108_15870 JD108_16400

PPY:

PPE_03281

PPM:	PPSC2_17355(moeB3)

PPO:	PPM_3501(moeB3)

PPOL:

X809_33395

PPQ:	PPSQR21_034980

PPOY:

RE92_19745

PTA:	HPL003_25965

PPEO:

ABE82_17455

PBV:	AR543_15185

PSWU:

SY83_04170

PCX:	LPB68_19285

PKB:	B4V02_07970

PAIH:

ASL14_20870

PVO:	PVOR_14549

PLUT:

EI981_11375

PBAC:

HUB98_03455

PSOP:

KP014_12365

PCEL:

HUB94_08575 HUB94_20590

PALR:

HGI30_16985

CCHL:

FPL14_22890

ACUR:

JZ785_17800

PANA:

BBH88_04740

JEO:

JMA_36380

SPSY:

AZE41_02005

SPOR:

SporoP33_10375

SPOP:

SporoP37_10790

SURE:

SporoP32a_10775

SPOS:

DV702_14925

SPAE:

E2C16_14615

SPOO:

J3U78_17550

RST:	ATY39_11655

PANC:

E2636_03685

LTA:	H1A07_03825

LBH:

Lbuc_2168

LBN:	LBUCD034_2274(thiF)

LPAR:

FAM21731_01229

LKF:	DNL43_06390

LHIL:

G8J22_02530

LPD:	AYR62_00060

PACI:

A4V11_02040

LME:

LEUM_1157

LMM:

MI1_05105

LMK:

LMES_0966

LKI:

LKI_04015

LEC:	LGMK_08355

WJO:	FOL01_1998

WPA:	CO680_08290(thiF)

WHE:	EFA59_09255(thiF)

ACG:	AWM71_00515 AWM71_06635

CAC:

CA_C2923

CAE:	SMB_G2959(ygdL)

CAY:

CEA_G2930

CPE:

CPE1602

CPF:	CPF_1855(thiF)

CPR:	CPR_1572(thiF)

CTC:	CTC_01750(moeB)

CTET:

BN906_01897(moeB)

CNO:	NT01CX_1503(thiF)

CBN:	CbC4_0676(thiF)

CBE:

Cbei_0647

CBZ:

Cbs_0647

CBEI:

LF65_00720

CKL:	CKL_1638(thiF)

CKR:

CKR_1523

CCB:	Clocel_0683

CLB:	Clo1100_2984

CSR:	Cspa_c49020(thiF1)

CSB:	CLSA_c25150(thiF)

CLT:	CM240_2383

CLD:	CLSPO_c21790(thiF)

CACE:

CACET_c37750(thiF2)

CCK:	Ccar_01320

CBUT:

ATN24_11555

CEU:	A7L45_01980

CFM:	BJL90_04605

CDRK:

B9W14_06305

CDY:	F3K33_03665(thiF)

CMAH:

C1I91_06860(thiF)

CRW:	CROST_037830

CFB:	CLFE_009900

AMT:

Amet_4236

CRS:	FQB35_00640(thiF)

CPRF:

K7H06_20255(thiF)

SARJ:

HH195_05675(thiF)

PBIF:

KXZ80_02645(thiF)

CLO:	HMPREF0868_0736

CTH:

Cthe_0600

CTX:	Clo1313_1629

CCL:	Clocl_2544

HSC:	HVS_07280(thiF)

CCEL:

CCDG5_1408

RAL:	Rumal_2156

FDU:	GXM22_03210

CCOM:

I6K69_08125(thiF)

BYL:	A4V09_20940

BHAN:

CGC63_13275(thiF)

BLAU:

DQQ01_05500

BPRO:

PMF13cell1_01241(tcdA_1)

CSCI:

HDCHBGLK_02706(moeB)

ARF:	AR1Y2_1224

ACAC:

EYQ97_11230(thiF)

CPRO:

CPRO_15970(moeB_1)

RTO:

RTO_25280

DLO:	K5I24_05455(thiF)

QDO:	H9Q78_01885(thiF)

WHJ:	H9Q79_09910(thiF)

CDF:	CD630_17030(thiF)

PDC:	CDIF630_01891(thiF)

CDC:	CD196_1627(thiF)

CDL:	CDR20291_1602(thiF)

PDF:	CD630DERM_17030(thiF)

EAC:	EAL2_c00340

TEM:	JW646_04970(thiF)

TEB:

T8CH_0611

SALQ:

SYNTR_2080

DAI:	Desaci_2489

DCA:	Desca_0063

PTH:

PTH_0642

DRM:

Dred_0075

DRU:	Desru_0137

DAE:

Dtox_1682

TFR:	BR63_18830(thiF)

DGI:	Desgi_0866

ELM:

ELI_0851

EMT:	CPZ25_017195

ELIM:

B2M23_11955

VGU:	HYG85_03360(thiF)

VPY:	HZI73_14335(thiF)

MDV:	C5Q96_03440

ETM:	CE91St48_39550(moeB)

EUU:	ADJ67_02415

TTE:	TTE2471(ThiF)

TEX:	Teth514_1158

THX:

Thet_1753

TIT:

Thit_0463

TMT:	Tmath_0554

TWI:	Thewi_2476

TKI:	TKV_c08380(thiF)

CHY:	CHY_1264(moeB)

TPZ:	Tph_c17850(thiF)

CSC:

Csac_0253

ATE:

Athe_0371

COB:	COB47_0338

CHD:	Calhy_2277

COW:	Calow_0241

CKI:	Calkr_0324

CLC:	Calla_2033

CKN:	Calkro_2257

CCHA:

ELD05_01275(thiF)

CDIA:

CaldiYA01_20700

HHL:	Halha_1309

AFT:	BBF96_07250

FMA:

FMG_0065

APR:

Apre_1633

AVG:	I6H45_07445(thiF)

AOB:	I6H46_01310(thiF)

PIV:	NCTC13079_01345(moeZ_2)

CAD:	Curi_c11650(thiF1)

KPAR:

JL105_02565(thiF)

CAZO:

G3A45_00580(thiF)

VPR:

Vpar_0458

MED:

MELS_2199

MHW:	ACT01_05960

MMAX:

LCQ47_04415(thiF)

MHG:

MHY_09380

MFUN:

GXM21_06365(thiF)

PUF:

UFO1_1305

AIN:	Acin_2102(thiF)

PFAC:

PFJ30894_00304(tcdA_1)

ACIT:

HPK19_14085

AUS:	IPK37_07475(thiF)

BAD:

BAD_0090

BADL:

BADO_0099

BADO:

BBMN23_0097

BDE:	BDP_0148(thiF)

BDN:

BBDE_0138

BTP:

D805_0147

BKA:	AH68_00550

BKS:

BBKW_0104

BCAT:

BBCT_0077

BANG:

BBAG_1425

BPSC:

BBPC_0090

BSCA:

BBSC_0176

BSUE:

BS3272_09765(thiF)

GVA:	HMPREF0424_0777(thiF)

ELE:

Elen_1748

EGD:	GS424_009980(thiF)

CGO:	Corgl_0460

VBS:	EGM51_15070(thiF)

RML:	FF011L_03260

BPIT:

BPIT_21420

JET:	L3J17_01480

PBU:	L21SP3_01124(moeZ)

PBP:	STSP1_01179(moeB_1)

PBAS:

SMSP2_01990(thiF)

ALUS:

STSP2_02472(moeB_1)

TAER:

GT409_15030(thiF)

VBC:	C5Q97_12485

STA:	STHERM_c01450

STQ:	Spith_0133

SPER:

EW093_14530(thiF)

ADIN:

H7849_06695

THYD:

TTHT_1303(thiF)

FNU:

FN1755

FNC:	HMPREF0946_01337

FNT:	HMPREF0405_00058

FUS:	HMPREF0409_01853

FNE:	FSDG_02324

FHW:	RN87_01275

FPD:	CTM68_07160(thiF)

FVA:	FV113G1_17900(thiF)

FUL:	C4N20_13290

FMO:	C4N19_10300

FGO:	C4N16_03840

FNF:	BSQ88_02555(thiF)

FPEI:

C4N17_00145

IPO:	Ilyop_1185

LOT:	AXF11_08180

LEQ:	BCB68_00280

LWD:	JCM16777_1475(thiF)

FSU:	Fisuc_2909

FSC:

FSU_0171

TTZ:	FHG85_01380(thiF)

BLQ:	L21SP5_00494(moeB)

CACI:

CLOAM1487

KOL:

Kole_0998

DDF:	DEFDS_1999(thiF)

DAP:	Dacet_0045

GTL:	EP073_02080(thiF)

CNI:	Calni_0070

FSI:	Flexsi_2169

MSCH:

N508_001672

DTH:	DICTH_1551(thiF)

DTU:

Dtur_1659

TAR:	TALC_00568

MAX:	MMALV_06010

MEAR:

Mpt1_c03090

MARC:

AR505_0103

» show all

Reference

1 [PMID:9632726]

Authors

Taylor SV, Kelleher NL, Kinsland C, Chiu HJ, Costello CA, Backstrom AD, McLafferty FW, Begley TP

Title

Thiamin biosynthesis in Escherichia coli. Identification of ThiS thiocarboxylate as the immediate sulfur donor in the thiazole formation.

Journal

J Biol Chem 273:16555-60 (1998)
DOI:10.1074/jbc.273.26.16555

Sequence

[eco:b3992]

Reference

2 [PMID:11438688]

Authors

Xi J, Ge Y, Kinsland C, McLafferty FW, Begley TP

Title

Biosynthesis of the thiazole moiety of thiamin in Escherichia coli: identification of an acyldisulfide-linked protein--protein conjugate that is functionally analogous to the ubiquitin/E1 complex.

Journal

Proc Natl Acad Sci U S A 98:8513-8 (2001)
DOI:10.1073/pnas.141226698

Sequence

[eco:b3992]

Reference

3 [PMID:15896804]

Authors

Duda DM, Walden H, Sfondouris J, Schulman BA

Title

Structural analysis of Escherichia coli ThiF.

Journal

J Mol Biol 349:774-86 (2005)
DOI:10.1016/j.jmb.2005.04.011

Sequence

[eco:b3992]

Reference

4 [PMID:16388576]

Authors

Lehmann C, Begley TP, Ealick SE.

Title

Structure of the Escherichia coli ThiS-ThiF complex, a key component of the sulfur transfer system in thiamin biosynthesis.

Journal

Biochemistry 45:11-9 (2006)
DOI:10.1021/bi051502y

Sequence

[eco:b3992]

Other DBs

ExplorEnz - The Enzyme Database:

2.7.7.73

IUBMB Enzyme Nomenclature:

2.7.7.73

ExPASy - ENZYME nomenclature database:

2.7.7.73

BRENDA, the Enzyme Database:

2.7.7.73

DBGET integrated database retrieval system