KEGG   ENZYME: 2.8.3.25
Entry
EC 2.8.3.25                 Enzyme                                 

Name
bile acid CoA-transferase;
baiF (gene name);
baiK (gene name);
bile acid coenzyme A transferase
Class
Transferases;
Transferring sulfur-containing groups;
CoA-transferases
Sysname
lithocholoyl-CoA:cholate CoA-transferase
Reaction(IUBMB)
(1) lithocholoyl-CoA + cholate = lithocholate + choloyl-CoA [RN:R11539];
(2) deoxycholoyl-CoA + cholate = deoxycholate + choloyl-CoA [RN:R07295]
Reaction(KEGG)
R07295 R11539
Substrate
lithocholoyl-CoA [CPD:C21449];
cholate [CPD:C00695];
deoxycholoyl-CoA [CPD:C15560]
Product
lithocholate [CPD:C03990];
choloyl-CoA [CPD:C01794];
deoxycholate [CPD:C04483]
Comment
The enzyme, characterized from the gut bacterium Clostridium scindens, catalyses the last step in bile acid 7alpha-dehydroxylation, the removal of the CoA moiety from the products. By using a transferase rather than hydrolase, the bacteria conserve the thioester bond energy, saving ATP molecules. Clostridium scindens possesses two forms of the enzyme, encoded by the baiF and baiK genes. While the enzymes have a broad acceptor specificity and can use allocholate, ursodeoxycholate, and beta-muricholate, the donor specificity is more strict. BaiF acts on lithocholoyl-CoA and deoxycholoyl-CoA, and BaiK acts only on the latter.
History
EC 2.8.3.25 created 2005 as EC 3.1.2.26, transferred 2016 to EC 2.8.3.25
Pathway
ec00121  Secondary bile acid biosynthesis
Orthology
K15871  bile acid CoA-transferase
Reference
1  [PMID:22021638]
  Authors
Ridlon JM, Hylemon PB
  Title
Identification and characterization of two bile acid coenzyme A transferases from Clostridium scindens, a bile acid 7alpha-dehydroxylating intestinal bacterium.
  Journal
J Lipid Res 53:66-76 (2012)
DOI:10.1194/jlr.M020313
  Sequence
Other DBs
ExplorEnz - The Enzyme Database: 2.8.3.25
IUBMB Enzyme Nomenclature: 2.8.3.25
ExPASy - ENZYME nomenclature database: 2.8.3.25
BRENDA, the Enzyme Database: 2.8.3.25

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