KEGG   ENZYME: 3.2.1.108
Entry
EC 3.2.1.108                Enzyme                                 
Name
lactase;
lactase-phlorizin hydrolase;
LPH (gene name);
LCT (gene name)
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
lactose galactohydrolase (configuration-retaining)
Reaction(IUBMB)
lactose + H2O = beta-D-galactose + D-glucose [RN:R01100 R06114]
Reaction(KEGG)
R01100 R06114(G);
(other) R01678 R06098(G)
Substrate
lactose [CPD:C00243];
H2O [CPD:C00001]
Product
beta-D-galactose [CPD:C00962];
D-glucose [CPD:C00031]
Comment
The enzyme from intestinal mucosa contains two glycosyl hydrolase domains, both of which belong to glycosyl hydrolase family 1 (GH1). While the first domain catalyses the activity described here, the second domain catalyses the reaction of EC 3.2.1.62 glycosylceramidase. cf. EC 3.2.1.33 amylo-alpha-1,6-glucosidase.
History
EC 3.2.1.108 created 1984, modified 2022
Pathway
ec00052  Galactose metabolism
ec01100  Metabolic pathways
Orthology
K01229  lactase-phlorizin hydrolase
Genes
HSA3938(LCT)
PTR459633(LCT)
PPS100993974(LCT)
GGO101136936(LCT)
PON100450303(LCT)
NLE100602088(LCT)
MCC707761(LCT)
MCF102141036(LCT)
MTHB126932258
MNI105492565(LCT)
CSAB103216991(LCT)
CATY105590352(LCT)
PANU101022382(LCT)
TGE112636379(LCT)
RRO104665436(LCT)
RBB108539586(LCT)
TFN117095069(LCT)
PTEH111527488(LCT)
CANG105508813(LCT)
CJC100408648(LCT)
SBQ101044226(LCT)
CSYR103263113(LCT)
MMUR105857603(LCT)
LCAT123643641(LCT)
PCOQ105815373(LCT)
OGA100944107(LCT)
MMU226413(Lct)
MCAL110304188(Lct)
MPAH110322545(Lct)
RNO116569(Lct)
MCOC116098602(Lct)
MUN110555417(Lct)
CGE100766856(Lct)
MAUA101828124(Lct)
PLEU114696961(Lct)
MORG121446300 121446324 121446359
MFOT126504990
AAMP119827729 119827814 119827830
NGI103734619(Lct)
HGL101697396(Lct)
CPOC100732160(Lct)
DORD105996629(Lct)
DSP122110822(Lct)
NCAR124980608
OCU100009256(LPH) 100352497 100353264
OPI101519618 101519868 101520351
TUP102470311(LCT)
GVR103610021(LCT)
CFA483898(LCT)
CLUD112649763(LCT)
VVP112932617(LCT)
VLG121481866(LCT)
AML100484153(LCT)
UMR103667712(LCT)
UAH113263420(LCT)
UAR123782596(LCT)
ELK111143551
LLV125095933
MPUF101676191(LCT)
NVS122902701(LCT)
ORO101373825(LCT)
EJU114207344(LCT)
ZCA113920630(LCT)
MLX118012500(LCT)
NSU110585741(LCT)
LWW102749114(LCT)
FCA101092452(LCT)
PYU121030495(LCT)
PBG122479372(LCT)
LRUF124516018
PTG102949288(LCT)
PPAD109270343(LCT)
AJU106969449(LCT)
HHV120230375(LCT)
BTA514332(LCT)
BOM102279578(LCT)
BIU109568203(LCT)
BBUB102410227(LCT)
BBIS104998486(LCT)
CHX102170472(LCT)
OAS101104042(LCT)
ODA120854489(LCT)
CCAD122453560(LCT)
SSC100625807(LCT) 100625897
CFR102513047(LCT)
CBAI105083393(LCT)
CDK105092305(LCT)
VPC102532468(LCT)
BACU103003658(LCT)
LVE103074579(LCT)
OOR101270599(LCT)
DLE111172712(LCT)
PCAD102988992(LCT)
PSIU116756492(LCT)
ECB100055369(LCT)
EPZ103548925(LCT)
EAI106836543(LCT)
MYB102246527(LCT)
MYD102761793(LCT)
MMYO118662079(LCT)
MLF102430551(LCT)
MNA107531139(LCT)
PKL118709739(LCT)
HAI109392715(LCT)
DRO112313063(LCT)
SHON118974532(LCT)
AJM119040600(LCT)
PDIC114495149(LCT)
PHAS123810272(LCT)
MMF118625706(LCT)
RFQ117025498(LCT)
PALE102887366(LCT)
PGIG120611943(LCT)
PVP105288339(LCT)
RAY107511200(LCT)
MJV108405363(LCT)
TOD119255787(LCT)
SARA101545875(LCT)
LAV100661599(LCT)
TMU101348169
ETF101659833(LCT)
DNM101441621(LCT)
MDO100016393(LCT)
GAS123241493(LCT)
SHR100933904(LCT)
PCW110223786(LCT)
OAA100084941(LCT)
GGA424294(LCT)
PCOC116227271(LCT)
MGP100540699(LCT)
CJO107316868(LCT)
NMEL110399364(LCT)
APLA101799746(LCT)
ACYG106039329(LCT)
AFUL116490699(LCT)
TGU100229941(LCT)
LSR110480639(LCT)
SCAN103814626(LCT)
PMOA120501755(LCT)
OTC121337155(LCT)
PRUF121356606(LCT)
GFR102041259(LCT)
FAB101814034(LCT)
PHI102100057(LCT)
PMAJ107207677(LCT)
CCAE111932317(LCT)
CCW104694217(LCT)
CBRC103619703(LCT)
ETL114057697(LCT)
ZAB102069527(LCT)
ACHL103797517(LCT)
FPG101921531(LCT)
FCH102058755(LCT)
CLV102086420(LCT)
EGZ104129535(LCT)
NNI104009760(LCT)
PLET104616147(LCT)
PCRI104026793(LCT)
PCAO104053764(LCT)
ACUN113482159(LCT)
TALA104368387(LCT)
PADL103912627(LCT)
AFOR103899524(LCT)
ACHC115342953(LCT)
HALD104323141(LCT)
HLE104836526(LCT)
AGEN126042537
GCL127018545
CCRI104157657(LCT)
CSTI104559163(LCT)
EHS104506311(LCT)
CMAC104479709(LCT)
MUI104545327(LCT)
FGA104076580(LCT)
GSTE104255215(LCT)
LDI104349434(LCT)
MNB103775982(LCT)
OHA104333255(LCT)
NNT104411659(LCT)
DPUB104296940(LCT)
PGUU104467528(LCT)
ACAR104532723(LCT)
CPEA104391143(LCT)
AVIT104277503(LCT)
CVF104287949(LCT)
CUCA104065226(LCT)
BRHI104488766(LCT)
AAM106498455(LCT)
AROW112977064(LCT)
NPD112948593(LCT)
TGT104565650(LCT)
DNE112988323(LCT)
SCAM104145714(LCT)
ASN102370133(LCT)
AMJ102576031(LCT)
CPOO109323207(LCT)
GGN109301357(LCT)
PSS102456860(LCT)
CMY102946778(LCT)
CPIC101933763(LCT)
TST117885118(LCT)
CABI116826106(LCT)
MRV120374328(LCT)
ACS103279188(lct)
PVT110071879(LCT)
SUND121925981(LCT)
PBI103054678(LCT)
PMUR107284386(LCT)
CTIG120307504(LCT)
TSR106540054(LCT)
PGUT117669655(LCT)
VKO123027553(LCT)
PMUA114604625(LCT)
ZVI118092494(LCT)
GJA107115411(LCT)
STOW125426237(LCT)
XLA100127248(lct.1.L) 100127291(lct.2.L)
XTR100145766(lct.2) 101732850
NPR108791336 108791337
RTEM120944145 120944146
BBUF121008829(LCT) 121008830
BGAR122944682 122944685
DRE559107 796436(lct)
SRX107739717 107741586
SANH107688954 107704822
SGH107601359 107601360
CAUA113108294 113108295
PPRM120485547 120487230
MAMB125245778 125245780 125270691
IPU108266116
PHYP113525948(lct)
SMEO124380192
TFD113654132
AMEX103029218(lct)
EEE113577223(lct)
TRU101076621
LCO104939725(lct)
NCC104954911(lct)
CGOB115026493(lct)
ELY117248075 117251770
EFO125899187 125899188
PLEP121949084 121949085
SLUC116044576 116044580
ECRA117953249
ESP116687473(lct)
PFLV114563686 114564430
GAT120834464 120834490
PPUG119229302
MSAM119903852
CUD121522156 121522288 121522602
ALAT119025751 119026172
MZE101465201 101465483
ONL100703330 100703603 100703875 102080755
OAU116324944 116324945 116324946 116333422
OLA101171223(lct)
OML112155270 112155271 112155272
XMA102216752 102217013 102223171(lct)
XCO114147565 114147571 114147572
XHE116722850 116722851 116723643(lct) 116723644
PRET103461729
PFOR103152094(lct) 103153521
PLAI106942509 106945899 106945900
PMEI106908439(lct) 106920698
GAF122840135 122840136
CVG107091021(lct) 107091024
CTUL119796496 119796544
GMU124870853 124870854
NFU107389384(lct)
ALIM106522267(lct)
NWH119418815
AOCE111581081 111586173
MCEP125017507 125017508 125017509 125017510 125017511 125017512 125017513
CSEM103392433(lct)
POV109631322
SSEN122759778 122759794
HHIP117755058
HSP118120062
SDU111218818
SLAL111667436
XGL120801628
HCQ109515508(lct)
MALB109966332(lct)
BSPL114847775
SASA106586119(lct)
STRU115160641(lct)
OTW112235127
OMY110519997
OGO124043772
ONE115107792(lct)
SALP111973682(lct)
SNH120057744
CCLU121536644
ELS105005862(lct)
SFM108928209(lct)
PKI111842887(lct)
AANG118222690
LOC102691495(lct)
PSPA121323070
ARUT117407121
PSEX120530640
LCM102361997(LCT)
CMK103180388
RTP109919181(lct)
BBEL109468489 109470924
SCLV120331019
BGT106067751 106069349 106069463
HRF124113779 124151882
HRJ124256256 124256662 124256664 124266902
CRG105317659 117692405
PMAX117332095
MMER123533272 123534954
TWL119987970
 » show all
Reference
1  [PMID:5822067]
  Authors
Asp NG, Dahlqvist A, Koldovsky O.
  Title
Human small-intestinal beta-galactosidases. Separation and characterization of one lactase and one hetero beta-galactosidase.
  Journal
Biochem J 114:351-9 (1969)
DOI:10.1042/bj1140351
Reference
2  [PMID:5010299]
  Authors
Schlegel-Haueter S, Hore P, Kerry KR, Semenza G.
  Title
The preparation of lactase and glucoamylase of rat small intestine.
  Journal
Biochim Biophys Acta 258:506-19 (1972)
DOI:10.1016/0005-2744(72)90242-2
Reference
3  [PMID:5082068]
  Authors
Lorenz-Meyer H, Blum AL, Haemmerli HP, Semenza G.
  Title
A second enzyme defect in acquired lactase deficiency: lack of small-intestinal phlorizin-hydrolase.
  Journal
Eur J Clin Invest 2:326-31 (1972)
DOI:10.1111/j.1365-2362.1972.tb00658.x
Reference
4  [PMID:810166]
  Authors
Ramaswamy S, Radhakrishnan AN.
  Title
Lactase-phlorizin hydrolase complex from monkey small intestine. Purification, properties and evidence for two catalytic sites.
  Journal
Biochim Biophys Acta 403:446-55 (1975)
DOI:10.1016/0005-2744(75)90072-8
Reference
5  [PMID:6786877]
  Authors
Skovbjerg H, Sjostrom H, Noren O.
  Title
Purification and characterisation of amphiphilic lactase/phlorizin hydrolase from human small intestine.
  Journal
Eur J Biochem 114:653-61 (1981)
DOI:10.1111/j.1432-1033.1981.tb05193.x
Reference
6  [PMID:6814489]
  Authors
Skovbjerg H, Noren O, Sjostrom H, Danielsen EM, Enevoldsen BS.
  Title
Further characterization of intestinal lactase/phlorizin hydrolase.
  Journal
Biochim Biophys Acta 707:89-97 (1982)
DOI:10.1016/0167-4838(82)90400-9
Reference
7  [PMID:9762914]
  Authors
Zecca L, Mesonero JE, Stutz A, Poiree JC, Giudicelli J, Cursio R, Gloor SM, Semenza G.
  Title
Intestinal lactase-phlorizin hydrolase (LPH): the two catalytic sites; the role of the pancreas in pro-LPH maturation.
  Journal
FEBS Lett 435:225-8 (1998)
DOI:10.1016/s0014-5793(98)01076-x
Reference
8  [PMID:11106409]
  Authors
Arribas JC, Herrero AG, Martin-Lomas M, Canada FJ, He S, Withers SG.
  Title
Differential mechanism-based labeling and unequivocal activity assignment of the two active sites of intestinal lactase/phlorizin hydrolase.
  Journal
Eur J Biochem 267:6996-7005 (2000)
DOI:10.1046/j.1432-1327.2000.01784.x
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.108
IUBMB Enzyme Nomenclature: 3.2.1.108
ExPASy - ENZYME nomenclature database: 3.2.1.108
BRENDA, the Enzyme Database: 3.2.1.108
CAS: 9031-11-2

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