KEGG   ENZYME: 3.2.1.130
Entry
EC 3.2.1.130                Enzyme                                 
Name
glycoprotein endo-alpha-1,2-mannosidase;
glucosylmannosidase;
endo-alpha-D-mannosidase;
endo-alpha-mannosidase;
endomannosidase;
glucosyl mannosidase;
MANEA (gene name);
glycoprotein glucosylmannohydrolase
Class
Hydrolases;
Glycosylases;
Glycosidases, i.e. enzymes that hydrolyse O- and S-glycosyl compounds
Sysname
glycoprotein glucosylmannohydrolase (configuration-retaining)
Reaction(IUBMB)
GlcMan9GlcNAc2-[protein] + H2O = Man8GlcNAc2-[protein] (isomer 8A1,2,3B1,2) + alpha-D-glucosyl-(1->3)-alpha-D-mannopyranose
Substrate
GlcMan9GlcNAc2-[protein];
H2O [CPD:C00001]
Product
Man8GlcNAc2-[protein] (isomer 8A1,2,3B1,2);
alpha-D-glucosyl-(1->3)-alpha-D-mannopyranose
Comment
The enzyme catalyses the hydrolysis of the terminal alpha-D-glucosyl-(1->3)-D-mannosyl unit from the GlcMan9(GlcNAc)2 oligosaccharide component of N-glucosylated proteins during their processing in the Golgi apparatus. The name for the isomer is based on a nomenclature proposed by Prien et al [7].
History
EC 3.2.1.130 created 1990, modified 2017
Orthology
K15538  glycoprotein endo-alpha-1,2-mannosidase
K21132  alpha-mannan endo-1,2-alpha-mannanase / glycoprotein endo-alpha-1,2-mannosidase
Genes
HSA79694(MANEA)
PTR472074(MANEA)
PPS100996027(MANEA)
GGO101153501(MANEA)
PON100174316(MANEA)
NLE100598520(MANEA)
MCC708164(MANEA)
MCF102131103(MANEA)
CSAB103240128(MANEA)
CATY105588962(MANEA)
PANU101018307(MANEA)
TGE112623791(MANEA)
RRO104668264(MANEA)
RBB108538601(MANEA)
TFN117097103(MANEA)
PTEH111538497(MANEA)
CJC100414257(MANEA)
SBQ101043274(MANEA)
CSYR103269474(MANEA)
MMUR105874023(MANEA)
OGA100946975(MANEA)
MMU242362(Manea)
MCAL110292995(Manea)
MPAH110338830(Manea)
RNO140808(Manea)
MCOC116088007(Manea)
MUN110565898(Manea)
CGE100682532(Manea)
PLEU114709930(Manea)
NGI103739270(Manea)
HGL101724745(Manea)
CPOC100718937(Manea)
CCAN109699781(Manea)
DORD105985935(Manea)
DSP122107747(Manea)
OCU100354038(MANEA)
OPI101529598(MANEA)
TUP102470618(MANEA)
CFA481925(MANEA)
VVP112923161(MANEA)
VLG121494525(MANEA)
AML100465796(MANEA)
UMR103675891(MANEA)
UAH113264815(MANEA)
UAR123798547(MANEA)
ELK111158292
LLV125102690
MPUF101685845(MANEA)
ORO101368286(MANEA)
EJU114209969(MANEA)
ZCA113927187(MANEA)
MLX118001424(MANEA)
FCA101101567(MANEA)
PYU121029795(MANEA)
PBG122489774(MANEA)
PTG102953225(MANEA)
PPAD109263429(MANEA)
AJU106980082(MANEA)
HHV120220542(MANEA)
BTA512853(MANEA)
BOM102266362(MANEA)
BIU109563524(MANEA)
BBUB102397661(MANEA)
CHX102190879(MANEA)
OAS101106778(MANEA)
ODA120862977(MANEA)
CCAD122422740(MANEA)
SSC100154996(MANEA)
CFR102505037(MANEA)
CBAI105063782(MANEA)
CDK105092263(MANEA)
VPC102533232(MANEA)
BACU102997965
LVE103075786(MANEA)
OOR101282781(MANEA)
DLE111163776(MANEA)
PCAD102977743(MANEA)
PSIU116763490(MANEA)
ECB100065922(MANEA)
EPZ103548352(MANEA)
EAI106832462(MANEA)
MYB102244343(MANEA)
MYD102761757(MANEA)
MMYO118660219(MANEA)
MLF102426767(MANEA)
MNA107542775(MANEA)
PKL118705764(MANEA)
HAI109373533(MANEA)
DRO112296961(MANEA)
SHON118982279(MANEA)
AJM119052085(MANEA)
PDIC114495181(MANEA)
PHAS123821762(MANEA)
MMF118621761(MANEA)
RFQ117020608(MANEA)
PALE102881260(MANEA)
PGIG120594768(MANEA)
PVP105292512(MANEA)
RAY107505443(MANEA)
MJV108387510(MANEA)
TOD119252706(MANEA)
SARA101547399(MANEA)
LAV100666598(MANEA)
TMU101343162
DNM101443971(MANEA)
MDO100026589(MANEA)
GAS123247597(MANEA)
SHR100925449(MANEA)
PCW110216463(MANEA)
OAA100073892(MANEA)
GGA421807(MANEA)
PCOC116240450(MANEA)
MGP100538669(MANEA)
CJO107311928(MANEA)
NMEL110395795(MANEA)
APLA101799379(MANEA)
ACYG106035412(MANEA)
AFUL116487243(MANEA)
TGU100227255(MANEA)
LSR110478567(MANEA)
SCAN103819054(MANEA)
PMOA120511589(MANEA)
OTC121340028(MANEA)
PRUF121359292(MANEA)
GFR102034556(MANEA)
FAB101815462(MANEA)
PHI102108129(MANEA)
PMAJ107201796(MANEA)
CCAE111925715(MANEA)
CCW104692141(MANEA)
ETL114062078(MANEA)
ZAB102068561(MANEA)
FPG101917050(MANEA)
FCH102050839(MANEA)
CLV102091008(MANEA)
EGZ104135406(MANEA)
NNI104021939(MANEA)
ACUN113478199(MANEA)
TALA104364678(MANEA)
PADL103913624(MANEA)
ACHC115333249(MANEA)
AAM106489716(MANEA)
AROW112971017(MANEA)
NPD112947157(MANEA)
DNE112996553(MANEA)
ASN102374994(MANEA)
AMJ102571324(MANEA)
CPOO109311874(MANEA)
GGN109299675(MANEA)
PSS102444522(MANEA)
CMY102943683(MANEA)
CPIC101940123(MANEA)
TST117875095(MANEA)
CABI116828688(MANEA)
MRV120400651(MANEA)
ACS100561214(manea)
PVT110074132(MANEA)
SUND121928732(MANEA)
PBI103065662(MANEA)
PMUR107294537(MANEA)
TSR106538554(MANEA)
PGUT117660778(MANEA)
VKO123022110(MANEA)
PMUA114593966(MANEA)
ZVI118082245(MANEA)
GJA107121220(MANEA)
XLA446517(manea.L)
XTR100485932(manea)
NPR108788904(MANEA)
RTEM120937124(MANEA)
BBUF120998198(MANEA)
BGAR122922858(MANEA)
DRE447827(manea)
SRX107751747 107755773
SANH107680802 107693121
SGH107564943 107583472
CCAR109069166 109069171
CAUA113060719 113071723 113078772
IPU108270006(manea)
PHYP113534085
SMEO124389710(manea)
TFD113651016(manea)
AMEX103022491
EEE113590640
TRU101078369
TNGGSTEN00031982G001
LCO104931611
NCC104964384(manea)
CGOB115027033(manea)
ELY117266707(manea)
PLEP121954045(manea)
SLUC116057593(manea)
ECRA117935605(manea)
PFLV114546760
GAT120833107(manea)
PPUG119227970(manea)
MSAM119909916(manea)
CUD121526024(manea)
MZE101476482
ONL100709960
OAU116326073(manea)
OLA101164688
OML112147972(manea)
XMA102223704(manea)
XCO114134734(manea)
XHE116708907(manea)
PRET103458747
PFOR103154973
PLAI106964680
PMEI106906599
GAF122845568
CVG107096905
CTUL119775087(manea)
GMU124884923(manea)
NFU107392157
KMR108233348(manea)
ALIM106518445 106537193
NWH119427875(manea)
AOCE111573274
CSEM103378701(manea)
POV109636231(manea)
SSEN122783482(manea)
HHIP117755517(manea)
LCF108874963(manea)
SDU111225671
SLAL111651003
XGL120794940(manea)
HCQ109517224
BPEC110153642
MALB109968006
SASA106561892
OTW112263438(manea)
OMY110506252(manea)
OGO124035937
ONE115113438
SALP111969169
SNH120024856(manea)
ELS105031619
SFM108933186
PKI111857975
AANG118207778(manea)
LOC102691324(manea)
PSPA121315458(manea)
ARUT117403064 117410572
LCM102347276(MANEA)
CMK103178280
RTP109927936
BFO118422672
BBEL109464637
SCLV120345699
SPU591903
APLC110986171
SKO100377430
AGAAgaP_AGAP011905
AAG110678383
AALB109410578 109421149
CQUCpipJ_CPIJ018073
CPII120431001
NVI100116153
CSOL105362854
TPRE106656890
MDL103576516
CGLO123258658
FAS105268886
DAM107041417
AGIF122853397
CCIN107274770
ATD109598528
AGB108917217
NVL108558901
PPYR116163693
BTAB109037547
CLEC106667568
HHAL106686935
NLU111060037
PHUPhum_PHUM600230
FOC113211220
ZNE110836294
CSEC111865826
DPXDAPPUDRAFT_216236
DMK116915644
EAF111704340
LOALOAG_11660
TSPTsp_10277
HROHELRODRAFT_132254
PCAN112566822
BGT106057620
GAE121370380
HRF124111840
HRJ124259947
CRG105334771
MYI110467305
PMAX117327825
OBI106874611
OSN115219846
LAK106155033
SMMSmp_016880
SHXMS3_0017952
NVE5512845
EPA110235549
ATEN116303163
ADF107347975
AMIL114959590
PDAM113672047
SPIS111335006
DGT114534807
XEN124441309 124442369
HMG100199440
TADTRIADDRAFT_31721
VCNVOLCADRAFT_99671
CVRCHLNCDRAFT_134058
ACANACA1_042010
SMINv1.2.001961.t1(symbB.v1.2.001961.t1)
FCYFRACYDRAFT_139403 FRACYDRAFT_149315 FRACYDRAFT_178285
AAFAURANDRAFT_2470 AURANDRAFT_2525 AURANDRAFT_29570
EHXEMIHUDRAFT_210275 EMIHUDRAFT_241315
GTTGUITHDRAFT_110280 GUITHDRAFT_117863
NGRNAEGRDRAFT_62591(FM155)
TVATVAG_040840 TVAG_117050 TVAG_137190 TVAG_150290 TVAG_297390 TVAG_319490
LABLA76x_2943(manEA)
LAQGLA29479_246
LMBC9I47_2704
LYJFKV23_02920
SAZSama_0297
SVLStrvi_5963
SMALSMALA_0936
STRCAA958_26800
SAUOBV401_08475
NPIG7071_01355
SROSros_3784
AMYYYIM_39930
PFLAPflav_004350
TTFTHTE_1595
PCORKS4_30670
MCADPan265_25160
PBPSTSP1_00024
ALUSSTSP2_03000
ABACLuPra_04144
BTHBT_3862
BTHOBtheta7330_00449
BXYBXY_34140
BFEK6V30_07760
PGSCPT03_06560
MUHHYN43_006815
MGKFSB76_30165
MRUBDEO27_007395
TMARMARIT_1314
AQBD1818_20890
AQAD1815_10990
AQDD1816_23440
CHZCHSO_4479
CGNOK18_05290
CARHEGY05_19145
CNKEG343_11535
CJTEG359_12225
CCAUEG346_09240
CBENEG339_03120
CGLENCTC11432_02793
 » show all
Reference
1  [PMID:3818665]
  Authors
Lubas WA, Spiro RG.
  Title
Golgi endo-alpha-D-mannosidase from rat liver, a novel N-linked carbohydrate unit processing enzyme.
  Journal
J Biol Chem 262:3775-81 (1987)
Reference
2  [PMID:2407194]
  Authors
Tulsiani DR, Coleman VD, Touster O.
  Title
Asparagine-linked glycoprotein biosynthesis in rat brain: identification of glucosidase I, glucosidase II, and and endomannosidase (glucosyl mannosidase).
  Journal
Arch Biochem Biophys 277:114-21 (1990)
DOI:10.1016/0003-9861(90)90558-G
Reference
3  [PMID:8106437]
  Authors
Hiraizumi S, Spohr U, Spiro RG
  Title
Ligand affinity chromatographic purification of rat liver Golgi endomannosidase.
  Journal
J Biol Chem 269:4697-700 (1994)
Reference
4  [PMID:9361017]
  Authors
Spiro MJ, Bhoyroo VD, Spiro RG
  Title
Molecular cloning and expression of rat liver endo-alpha-mannosidase, an N-linked oligosaccharide processing enzyme.
  Journal
J Biol Chem 272:29356-63 (1997)
DOI:10.1074/jbc.272.46.29356
  Sequence
[rno:140808]
Reference
5  [PMID:15677381]
  Authors
Hamilton SR, Li H, Wischnewski H, Prasad A, Kerley-Hamilton JS, Mitchell T, Walling AJ, Davidson RC, Wildt S, Gerngross TU
  Title
Intact {alpha}-1,2-endomannosidase is a typical type II membrane protein.
  Journal
Glycobiology 15:615-24 (2005)
DOI:10.1093/glycob/cwi045
  Sequence
[hsa:79694] [rno:140808]
Reference
6  [PMID:15760709]
  Authors
Hardt B, Volker C, Mundt S, Salska-Navarro M, Hauptmann M, Bause E
  Title
Human endo-alpha1,2-mannosidase is a Golgi-resident type II membrane protein.
  Journal
Biochimie 87:169-79 (2005)
DOI:10.1016/j.biochi.2004.11.004
  Sequence
[hsa:79694]
Reference
7  [PMID:19181540]
  Authors
Prien JM, Ashline DJ, Lapadula AJ, Zhang H, Reinhold VN
  Title
The high mannose glycans from bovine ribonuclease B isomer characterization by ion trap MS.
  Journal
J Am Soc Mass Spectrom 20:539-56 (2009)
DOI:10.1016/j.jasms.2008.11.012
Other DBs
ExplorEnz - The Enzyme Database: 3.2.1.130
IUBMB Enzyme Nomenclature: 3.2.1.130
ExPASy - ENZYME nomenclature database: 3.2.1.130
BRENDA, the Enzyme Database: 3.2.1.130
CAS: 108022-16-8

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