KEGG   ENZYME: 3.2.2.5
Entry
EC 3.2.2.5                  Enzyme                                 
Name
NAD+ glycohydrolase;
NAD glycohydrolase;
nicotinamide adenine dinucleotide glycohydrolase;
beta-NAD+ glycohydrolase;
DPNase (ambiguous);
NAD hydrolase (ambiguous);
diphosphopyridine nucleosidase (ambiguous);
nicotinamide adenine dinucleotide nucleosidase (ambiguous);
NAD nucleosidase (ambiguous);
DPN hydrolase (ambiguous);
NADase (ambiguous);
nga (gene name);
NAD+ nucleosidase
Class
Hydrolases;
Glycosylases;
Hydrolysing N-glycosyl compounds
Sysname
NAD+ glycohydrolase
Reaction(IUBMB)
NAD+ + H2O = ADP-D-ribose + nicotinamide [RN:R00102]
Reaction(KEGG)
R00102
Substrate
NAD+ [CPD:C00003];
H2O [CPD:C00001]
Product
ADP-D-ribose [CPD:C00301];
nicotinamide [CPD:C00153]
Comment
This enzyme catalyses the hydrolysis of NAD+, without associated ADP-ribosyl cyclase activity (unlike the metazoan enzyme EC 3.2.2.6, bifunctional ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase). The enzyme from Group A streptococci has been implicated in the pathogenesis of diseases such as streptococcal toxic shock-like syndrome (STSS) and necrotizing fasciitis. The enzyme from the venom of the snake Agkistrodon acutus also catalyses EC 3.6.1.5, apyrase [3].
History
EC 3.2.2.5 created 1961, modified 2013
Pathway
ec00760  Nicotinate and nicotinamide metabolism
ec01100  Metabolic pathways
Orthology
K18153  NAD+ glycohydrolase
K19980  ADP-ribosyltransferase 2 / NAD+ glycohydrolase
Genes
MMU11871(Art2a) 11872(Art2b)
MCAL110298812 110298813
MPAH110336159
RNO293152(Art2b)
MCOC116100665 116101301
MUN110550800
CGE100750708 100751294 100771938 100772218 100772803
MAUA101824175 101839372 121134115 121144298
PLEU114698648 114698743 114698744 114706661 119086245
MFOT126488848
NGI103748863 103749849
HGL106007650
CPOC100714269 100717061
CCAN109678990
NCAR124958806
OPI101536559
TUP102487179
BACU103000152
MYB102252898
MYD102773420
MMYO118664685 118664687
MLF102425244 102438022 102438936
MNA107533657
DRO112315853
SHON119002660
AJM119042110
PDIC114498648 114500760
PHAS123805524 123816978
MMF118627508
RFQ117031024
PALE102895427
PGIG120590522
PVP105303689
SARA101541382
DNM111761064
FCD110841647
SPYSPy_0165(nga)
SPZM5005_Spy0139(nga)
SPYMM1GAS476_0178(nga)
SPYAA20_0190(nga)
SPMspyM18_0163(nga)
SPGSpyM3_0128(nga)
SPSSPs0130
SPHMGAS10270_Spy0141(nga)
SPIMGAS10750_Spy0145(nga)
SPJMGAS2096_Spy0146(nga)
SPKMGAS9429_Spy0141(nga)
SPFSpyM50134(nga)
SPAM6_Spy0186
SPBM28_Spy0137(nga)
STGMGAS15252_0175(nga)
STXMGAS1882_0175(nga)
SOZSpy49_0144(nga)
STZSPYALAB49_000178
SPYHL897_00945
SPYOSTAB901_00975
SEUSEQ_0311
SDSSDEG_2029(nga)
SDGSDE12394_10265
SDAGGS_1869(nga)
SDCSDSE_2125(nga)
SDQSDSE167_2131(nga)
SCAINCTC12191_01452
 » show all
Reference
1  [PMID:4322210]
  Authors
Fehrenbach FJ
  Title
[Purification and crystallization of NAD glycohydrolase of C streptococci].
  Journal
Eur J Biochem 18:94-102 (1971)
DOI:10.1111/j.1432-1033.1971.tb01219.x
Reference
2  [PMID:236282]
  Authors
Grushoff PS, Shany S, Bernheimer AW
  Title
Purification and properties of streptococcal nicotinamide adenine dinucleotide glycohydrolase.
  Journal
J Bacteriol 122:599-605 (1975)
DOI:10.1128/JB.122.2.599-605.1975
Reference
3  [PMID:18952139]
  Authors
Zhang L, Xu X, Luo Z, Shen D, Wu H
  Title
Identification of an unusual AT(D)Pase-like activity in multifunctional NAD glycohydrolase from the venom of Agkistrodon acutus.
  Journal
Biochimie 91:240-51 (2009)
DOI:10.1016/j.biochi.2008.09.003
Reference
4  [PMID:20018886]
  Authors
Ghosh J, Anderson PJ, Chandrasekaran S, Caparon MG
  Title
Characterization of Streptococcus pyogenes beta-NAD+ glycohydrolase: re-evaluation of enzymatic properties associated with pathogenesis.
  Journal
J Biol Chem 285:5683-94 (2010)
DOI:10.1074/jbc.M109.070300
Reference
5  [PMID:21300288]
  Authors
Smith CL, Ghosh J, Elam JS, Pinkner JS, Hultgren SJ, Caparon MG, Ellenberger T
  Title
Structural basis of Streptococcus pyogenes immunity to its NAD+ glycohydrolase toxin.
  Journal
Structure 19:192-202 (2011)
DOI:10.1016/j.str.2010.12.013
Other DBs
ExplorEnz - The Enzyme Database: 3.2.2.5
IUBMB Enzyme Nomenclature: 3.2.2.5
ExPASy - ENZYME nomenclature database: 3.2.2.5
BRENDA, the Enzyme Database: 3.2.2.5
CAS: 9025-46-1

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