KEGG   ENZYME: 3.5.1.14
Entry
EC 3.5.1.14                 Enzyme                                 
Name
N-acyl-aliphatic-L-amino acid amidohydrolase;
aminoacylase 1;
aminoacylase I;
dehydropeptidase II;
histozyme;
hippuricase;
benzamidase;
acylase I;
hippurase;
amido acid deacylase;
L-aminoacylase;
acylase;
aminoacylase;
L-amino-acid acylase;
alpha-N-acylaminoacid hydrolase;
long acyl amidoacylase;
short acyl amidoacylase;
ACY1 (gene name);
N-acyl-L-amino-acid amidohydrolase
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
N-acyl-aliphatic-L-amino acid amidohydrolase (carboxylate-forming)
Reaction(IUBMB)
(1) an N-acyl-aliphatic-L-amino acid + H2O = an aliphatic L-amino acid + a carboxylate [RN:R01263];
(2) an N-acetyl-L-cysteine-S-conjugate + H2O = an L-cysteine-S-conjugate + acetate [RN:R10553]
Reaction(KEGG)
R01263 > R00669;
R10553
Substrate
N-acyl-aliphatic-L-amino acid;
H2O [CPD:C00001];
N-acetyl-L-cysteine-S-conjugate
Product
aliphatic L-amino acid;
carboxylate [CPD:C00060];
L-cysteine-S-conjugate [CPD:C02882];
acetate [CPD:C00033]
Comment
Contains Zn2+. The enzyme is found in animals and is involved in the hydrolysis of N-acylated or N-acetylated amino acids (except L-aspartate). It acts on mercapturic acids (S-conjugates of N-acetyl-L-cysteine) and neutral aliphatic N-acyl-alpha-amino acids. Some bacterial aminoacylases demonstrate substrate specificity of both EC 3.5.1.14 and EC 3.5.1.114. cf. EC 3.5.1.15, aspartoacylase and EC 3.5.1.114, N-acyl-aromatic-L-amino acid amidohydrolase.
History
EC 3.5.1.14 created 1965, modified 2013
Pathway
ec00220  Arginine biosynthesis
ec01100  Metabolic pathways
ec01110  Biosynthesis of secondary metabolites
Orthology
K14677  aminoacylase
Genes
HSA100526760(ABHD14A-ACY1) 95(ACY1)
PTR460414(ACY1)
PPS100989711(ACY1)
PON100171519(ACY1)
NLE100603023
MCC100425407 698851
MCF101867505
CSAB103227712(ACY1)
CATY105574295(ACY1)
PANU101019806
TGE112619217
RRO104663721 115898588
TFN117082568(ACY1)
PTEH111528789 113219581 113223178 113223179
CJC100386117(ACY1)
SBQ101027476(ACY1)
CSYR103260142(ACY1)
MMUR105875031(ACY1)
OGA100943762(ACY1)
MMU109652(Acy1)
MCAL110301037(Acy1)
MPAH110327471
RNO300981(Acy1)
MCOC116072453(Acy1)
MUN110550172(Acy1)
CGE100765707
PLEU114710963(Acy1)
NGI103747435(Acy1)
HGL101696409(Acy1)
CPOC100714326
CCAN109685975(Acy1)
DORD105994617(Acy1)
DSP122095742
OCU100344479(ACY1)
OPI101520640
TUP102503256(ACY1)
CFA476602
VVP112910690
VLG121494440(ACY1)
AML100479661
UMR103675074(ACY1)
UAH113256935
UAR123798716
ELK111143997
LLV125079256
MPUF101678773(ACY1)
ORO101372032(ACY1)
EJU114225067
ZCA113917280
MLX118006024
PYU121025148
PBG122487287
PTG102948715(ACY1)
PPAD109247881
AJU106987018
HHV120239411
BTA768058(ACY1)
BOM102274671(ACY1)
BIU109576040(ACY1)
BBUB102408788(ACY1)
CHX102173979(ACY1)
OAS101113801(ACY1)
ODA120868339(ACY1)
CCAD122424698(ACY1)
SSC396930(ACY1)
CFR102512036
CBAI105080194(ACY1)
CDK105093821(ACY1)
VPC102529518
BACU103005903(ACY1)
LVE103082569(ACY1)
OOR101286870(ACY1)
DLE111174327(ACY1)
PCAD102982112 112064954
PSIU116762264(ACY1)
ECB100060631(ACY1)
EPZ103546483(ACY1)
EAI106821753
MYB102263075(ACY1)
MYD102753333(ACY1)
MMYO118668420(ACY1)
MLF102430285(ACY1)
MNA107540194(ACY1)
PKL118715612
HAI109375828(ACY1)
DRO112309817
SHON118989417(ACY1)
AJM119036616
PDIC114502048(ACY1)
PHAS123830600(ACY1)
MMF118633130(ACY1)
RFQ117036570(ACY1)
PALE102885238(ACY1)
PGIG120582410(ACY1)
PVP105295124(ACY1)
RAY107504158(ACY1)
MJV108388575(ACY1)
TOD119260910(ACY1)
SARA101552481(ACY1)
LAV100663232
TMU101349746
DNM101412378
MDO100031141(ACY1)
GAS123246081
SHR100934680(ACY1)
PCW110217043(ACY1)
OAA100075293
GGA100858836(ACY1)
PCOC116241064
MGP100550404(ACY1)
CJO107319637
NMEL110405016(ACY1)
APLA101803141
ACYG106042853(ACY1)
AFUL116492932
TGU100219242
LSR110472111
SCAN103824272
PMOA120495689
OTC121347401
PRUF121351117
GFR102033439(ACY1)
FAB101809806(ACY1)
PHI102107169(ACY1)
PMAJ107210075
CCAE111935148(ACY1)
CCW104683245
ETL114058684
ZAB102066246
FPG101918277(ACY1)
FCH102057393
CLV102085226
EGZ104126741(ACY1)
NNI104008693(ACY1)
ACUN113484690(ACY1)
TALA104361246
PADL103915051(ACY1)
ACHC115353853(ACY1)
AAM106487065(ACY1)
AROW112968833(ACY1)
NPD112953100
DNE112986955
ASN102369149(ACY1)
AMJ102566953(ACY1)
GGN109292714(ACY1)
PSS102455304(ACY1)
CMY102938259(ACY1)
CPIC101950834(ACY1)
TST117880489(ACY1)
CABI116831336(ACY1)
MRV120409215(ACY1)
ACS100567123(acy1)
PVT110084475(ACY1)
SUND121920958(ACY1)
PBI103050622(ACY1)
PMUR107291116(ACY1)
TSR106557154(ACY1)
PGUT117678529(ACY1)
VKO123034292(ACY1)
PMUA114591568(ACY1)
ZVI118080610(ACY1)
GJA107115931(ACY1)
XLA399011(acy1.2.L) 446741(acy1.1.S)
XTR101735201 496883(acy1.1)
NPR108793445
RTEM120944933(ACY1)
BBUF120978633
BGAR122943534(ACY1)
DRE100001620 103909643 393970(acy1)
SRX107747934 107752189
SANH107655273 107698043
SGH107550684 107575257
CCAR109047323
CAUA113039794(acy1) 113040498
IPU108254950
PHYP113539457(acy1)
SMEO124402449
AMEX103031539(acy1)
EEE113583811(acy1)
TRU101072726(acy1)
TNGGSTEN00027408G001
LCO104931347(acy1)
NCC104967510(acy1)
CGOB115008844(acy1)
ELY117256181
PLEP121961092
SLUC116041589
ECRA117943525
PFLV114554745(acy1)
GAT120835815
PPUG119229954
MSAM119885960
CUD121507518
MZE101471948(acy1)
ONL100694810(acy1)
OAU116313668
OLA101160548(acy1)
OML112145289
XMA102227096(acy1)
XCO114135714(acy1)
XHE116710200
PRET103464610(acy1)
PFOR103137960(acy1)
PLAI106962485(acy1)
PMEI106914943(acy1)
GAF122834516
CVG107102904(acy1)
CTUL119779981
GMU124856080
NFU107385138(acy1)
KMR108247369
ALIM106516071(acy1)
NWH119412469
AOCE111563222(acy1)
CSEM103385797(acy1)
POV109638451(acy1)
SSEN122768796
HHIP117761139
LCF108889386(acy1)
SDU111222940(acy1)
SLAL111660709(acy1)
XGL120783183
HCQ109522261(acy1)
BPEC110168627(acy1)
MALB109952383(acy1)
SASA100194932(acy1)
OTW112214706
OMY110494017
OGO124003713
ONE115142734(acy1)
SALP112070374(acy1)
SNH120063802
ELS105028113(acy1)
SFM108933832(acy1)
PKI111860026(acy1)
AANG118211860
LOC102696507(acy1)
PSPA121329388
ARUT117411779 117971353
LCM102363836(ACY1)
CMK103176752
RTP109923199(acy1)
BFO118416611
BBEL109467122
CIN100177060 100185588
SCLV120330375 120334015 120343054 120343197 120343198
SPU115918107 577570 578000
APLC110984341
SKO100377599 102801074
DMEDmel_CG17109(CG17109) Dmel_CG17110(CG17110) Dmel_CG6465(CG6465) Dmel_CG6726(CG6726) Dmel_CG6733(CG6733) Dmel_CG6738(CG6738)
DER6551980 6554750 6554751 6554781 6554782
DSE6606940 6606941 6607531 6607532 6607533 6607534
DSIDsimw501_GD20716(Dsim_GD20716) Dsimw501_GD20717(Dsim_GD20717) Dsimw501_GD20996(Dsim_GD20996) Dsimw501_GD20998(Dsim_GD20998) Dsimw501_GD20999(Dsim_GD20999) Dsimw501_GD21000(Dsim_GD21000) Dsimw501_GD21001(Dsim_GD21001)
DYADyak_GE10351 Dyak_GE10352 Dyak_GE10353 Dyak_GE10354 Dyak_GE10355 Dyak_GE24682 Dyak_GE24683
DAN6499765 6499766 6499767 6499768 6500302
DSR110191004 110191006 110191023 110191242 110191243 110191244 110191245 110191246 110191359
DPODpse_GA19815 Dpse_GA19818 Dpse_GA26984 Dpse_GA27068 Dpse_GA27069 Dpse_GA29129 Dpse_GA29130
DPE6588033 6588046 6595081 6595082 6595083
DMN108156815 108156816 108156818 108157010 108157011
DWI6649841 6649842 6650512 6650513 6650514 6650624
DGR6567872 6567873 6567874 6570878
DMODmoj_GI18128 Dmoj_GI23017 Dmoj_GI23018 Dmoj_GI23019 Dmoj_GI23020
DAZ108611518 108621151 108621152 108621153
DNV108659996 108659997 108659998 108659999 115564591
DHE111599611 111599613 111599614 111599619 111600558
DVI6627508 6630824 6630825 6630956
CCAT101455350 101455522 101455689 101455990
BOD106615864 106615890
MDE101897431 101900051 101900167 101900512
SCAC106087402 106087411
LCQ111680294 111680298 111680303 111680317
AGAAgaP_AGAP000679
ACOZ120955387
AARA120905887
AAG5574529
AALB109397278 109405345 109423208 115254536 115255644
CQUCpipJ_CPIJ009804 CpipJ_CPIJ013669 CpipJ_CPIJ013670 CpipJ_CPIJ013671
CPII120421076 120426017 120426018 120426019
CNS116341916 116341917 116341918 116341944 116341995 116342029 116342070 116342071 116342180 116342182 116344725
AME408969
ACER107993607
ALAB122717031
BIM100746427
BBIF117216864
BVK117236511
BVAN117154557
BTER100645222
BPYO122576424
CCAL108622570
OBB114876678
MGEN117220922
NMEA116425841
SOC105207857
MPHA105839002
AEC105154436
ACEP105621043
PBAR105428375
VEM105569472 105569473
HST105183201 105183202 105191364
DQU106745603 106746890
CFO105252213 105254159 109610062
FEX115238716
LHU105669821
PGC109860040
OBO105276644
PCF106788996
PFUC122514608
VPS122634166
NVI100114283
CSOL105359781
TPRE106658196
MDL103571230
CGLO123260466
FAS105263208
DAM107039965
AGIF122848000 122848393 122848968
CCIN107270550
TCA657516 657592 657672
DPA109540108
SOY115874963
ATD109594510 109595479
CSET123312943 123313320
AGB108908583
LDC111502633
NVL108556952 108564069 108564074
APLN108734169
PPYR116161901 116161911 116162037 116162500 116164352 116168720 116169314 116175360 116175491 116175909 116175910 116176242 116176638 116176772 116176794 116181156
OTU111421048
BMOR101742389 101742637 101745900 101746001 101746161 101746807
BMAN114246094 114246984 114248662 114253609
MSEX115441320 115447653 115449340 115449346 115454371
DPLKGM_205806 KGM_209240A KGM_213685
BANY112044749 112045060 112058061
PMAC106711796 106714707 106714708 106714888 106714916 106715066 106717664 106719537 106721337
PPOT106101948 106101949 106101950 106101952 106101987 106101992 106101996 106102026 106102027 106102053 106102439 106105700 106106778
PXU106122141 106123568 106123576 106125456 106125960 106126068 106126077 106126088 106127847 106127864
PRAP110993247 110994002 110999680
ZCE119833720 119834323 119834473
HAW110370672 110370673 110370749 110374988 110376077 110384378
TNL113497189 113497408 113497610 113502487 113505804
OFU114354272 114358927 114359563 114364278
PXY105381157 105384203 105384232 105386459 105386460 105390016 105391324 105392295 105392298 105396315 105396706 105396707
API100162258 100165297 100169089
DNX107171543 107171544
AGS114123475 114123476 114123487
RMD113548242 113551944 113556514
BTAB109043553
DCI103513711
CLEC106670786
HHAL106692067 106692624 106692625 106692629 106692630
NLU111057758
PHUPhum_PHUM047660
FOC113214887
ZNE110832721
CSEC111861965 111875594
FCD110845065 110846477 110846510 110849901 110853823 110863528
DPXDAPPUDRAFT_48442
DMK116924907
PVM113810372 113813709
PJA122247043 122260464
HAME121854745 121854791 121859454 121859456
PCLA123745691 123762308
PTRU123508561 123514320
HAZT108681027
EAF111703871
CELCELE_C06A6.4(C06A6.4) CELE_C10C5.3(C10C5.3) CELE_C10C5.4(C10C5.4) CELE_C10C5.5(C10C5.5)
CBRCBG_17687 CBG_24539
BMYBM_BM17575(Bm17575)
LOALOAG_18850
NAINECAME_02060 NECAME_15660
TSPTsp_07914
HROHELRODRAFT_62927
LGILOTGIDRAFT_197114 LOTGIDRAFT_222505
PCAN112571574
BGT106052797 106052798
GAE121368078 121392474
HRF124133831 124133873
HRJ124289903 124289905
CRG105336810 117682059
MYI110443347
PMAX117330611
MMER123529625
OBI106878078
OSN115217582 115231172
LAK106151703 106160986
SMMSmp_019630
OVIT265_08174 T265_14495
EGLEGR_04365
ADF107353293
AMIL114947117
PDAM113673272
SPIS111322407
TADTRIADDRAFT_50344
AQU100634447
ATHAT1G44180 AT1G44820 AT4G38220(AQI)
ALY9304911 9315618 9317463 9330044 9330057
CRB17880285 17887806 17897659 17899585
CSAT104716103 104716105 104719897 104729133 104742184 104757947 104777562 104777586 104781947 104786010 109124733
EUSEUTSA_v10011489mg EUTSA_v10012140mg EUTSA_v10016717mg EUTSA_v10025205mg
BRP103827501 103828110 103847925
BNA106350679 106350870 106404689 106426515 106433265 106434002 106435544 106438617
BOE106300828 106303509 106309201 106335773
RSZ108813541 108836566 108842805 108846190 108849891 108854796
THJ104817223 104824731
CPAP110816746 110821496
CIT102611318 102622833
CICCICLE_v10028440mg
PVY116110645 116119343 116132915
MINC123203623 123206126 123213983
TCC18592308 18608383
GRA105779666 105782851 105786149 105795027
GHI107894009 107898901 107901663 107907642 107907691 107908978 107920439 107922227 107942367 107944751 107953104 107963849 121215950 121229426
GAB108457661 108460705 108464322 108474591
DZI111282920 111315623
EGR104419053 104450949
GMX100783172 100817383
GSJ114382181 114403160
PVUPHAVU_003G001200g PHAVU_008G164800g
VRA106753643 106765126
VAR108335972 108338118
VUN114173929 114194525
CCAJ109815996 109817938
APRC113846844 113868729 113868802
MTRMTR_1g038920 MTR_1g039010 MTR_5g058430
CAM101489624
LJALj0g3v0037669.1(Lj0g3v0037669.1) Lj0g3v0313609.1(Lj0g3v0313609.1) Lj0g3v0313609.2(Lj0g3v0313609.2) Lj0g3v0313609.3(Lj0g3v0313609.3)
ADU107470122 107485079
AIP107622734 107639661
AHF112715589 112743741 112797111
LANG109356547 109360836
FVE101297614 101306972
RCN112172056 112173713 112184277
PPER18767701 18792444
PMUM103319947 103334632
PAVI110753256 110754679
PDUL117616560 117638599
MDM103440917 103440951 103445495
PXB103932870 103936000 103949509
ZJU107406028 107425640 107426535 107435996
MNT21386492 21396903 21403086
CSV101213747
CMO103490443 103496230
BHJ120069053 120071486
MCHA111011275 111011881
CMAX111469806 111487760
CMOS111451263 111464784
CPEP111794899 111811272
RCU8274101 8285377
JCU105638915
HBR110638919 110665147
MESC110608766 110610477 110610478
POP18096216 18098169 7463166 7463357
PEU105108297 105127919 105136990
PALZ118032276 118038880 118040921 118040930
JRE108988347 108997411
QSU112003024 112032954
QLO115960364 115968776 115969487 115969489
TWL119981547 120010875
VVI100257526 100260562
VRI117907410 117911867
SLY101248259 543751
SPEN107005038 107027000
SOT102590216 102593106
CANN107840659 107868075
NTA107768780 107774716 107817856(NtACY) 107828516
NSY104224953 104242920
NTO104103369 104112374
NAU109207749 109239313
INI109148830 109170791
ITR116011328 116015563 116016317 116031944 116032546
SIND105159481 105172986
OEU111393386
EGT105953697 105954674 105973033
SSPL121741959 121745000 121758150
HAN110864297 110917191
ECAD122579155 122584840 122601374 122609499
LSV111896192 111896194
CCAV112501938 112527698
DCR108197289 108221895 108224269
CSIN114267349 114278969
BVG104904088 104904089 104904090 104904092 104906914
SOE110786634 110786638 110786639 110786640 110795035
CQI110682012 110682044 110682076 110718685 110718687 110718689 110718702 110718954 110725346
NNU104606852 104608485
MING122073972 122073974 122075255 122084700
TSS122659862 122662214 122662713 122662714 122662715 122662798 122662799
PSOM113283129 113287071 113289662 113299177 113299187
NCOL116257676 116257722
OSA4340457 4346012
DOSAOs06t0210200-01(Os06g0210200) Os08t0511900-01(Os08g0511900)
OBR102711640 102713551
BDI100826836 100839108
ATS109742087 109779347
TDC119328664 119329542 119336964 119337630
TAES123148906 123151271 123157518 123159172 123165396 123170358
SBI8055167 8077344
ZMA100282205 100283955
SITA101779195 101785165
SVS117852436 117860640
PVIR120669764 120678342 120704629 120712824
PHAI112888652 112897073
PDA103697173 103705188 120105761
EGU105052838 105060929
MUS103976998 103989500
DCT110099803 110100754 110101417
PEQ110026318 110031913 110034827
AOF109841919 109846270
ATR18425845 18435830 18439756
SMOSELMODRAFT_120255 SELMODRAFT_406809
PPP112284932 112295617
CMECYME_CMA011C
GSLGasu_22570
CCPCHC_T00000215001
MBRMONBRDRAFT_32786
SREPTSG_01855
DDIDDB_G0270562(acy1)
DPPDICPUDRAFT_53427
DFADFA_03140(acy1)
ACANACA1_156080
SMINv1.2.025325.t1(symbB.v1.2.025325.t1)
PTIPHATRDRAFT_15083
FCYFRACYDRAFT_181231
TPSTHAPSDRAFT_15114
PIFPITG_00028 PITG_00029 PITG_02852
PSOJPHYSODRAFT_344735 PHYSODRAFT_349947 PHYSODRAFT_358729
SPARSPRG_01415
NGRNAEGRDRAFT_77264 NAEGRDRAFT_81120
 » show all
Reference
1  [PMID:14927637]
  Authors
BIRNBAUM SM, LEVINTOW L, KINGSLEY RB, GREENSTEIN JP.
  Title
Specificity of amino acid acylases.
  Journal
J Biol Chem 194:455-70 (1952)
Reference
2  [PMID:13061423]
  Authors
FONES WS, LEE M.
  Title
Hydrolysis of N-acyl derivatives of alanine and phenylalanine by acylase I and carboxypeptidase.
  Journal
J Biol Chem 201:847-56 (1953)
Reference
3  [PMID:3355856]
  Authors
Henseling J, Rohm KH
  Title
Aminoacylase I from hog kidney: anion effects and the pH dependence of kinetic parameters.
  Journal
Biochim Biophys Acta 959:370-7 (1988)
DOI:10.1016/0005-2760(88)90211-1
Reference
4  [PMID:2318199]
  Authors
Heese D, Berger S, Rohm KH
  Title
Nuclear magnetic relaxation studies of the role of the metal ion in Mn2(+)-substituted aminoacylase I.
  Journal
Eur J Biochem 188:175-80 (1990)
DOI:10.1111/j.1432-1033.1990.tb15385.x
Reference
5  [PMID:7662111]
  Authors
Palm GJ, Rohm KH
  Title
Aminoacylase I from porcine kidney: identification and characterization of two major protein domains.
  Journal
J Protein Chem 14:233-40 (1995)
DOI:10.1007/bf01886764
Reference
6  [PMID:9671543]
  Authors
Uttamsingh V, Keller DA, Anders MW
  Title
Acylase I-catalyzed deacetylation of N-acetyl-L-cysteine and S-alkyl-N-acetyl-L-cysteines.
  Journal
Chem Res Toxicol 11:800-9 (1998)
DOI:10.1021/tx980018b
Reference
7  [PMID:10727768]
  Authors
Lindner H, Hopfner S, Tafler-Naumann M, Miko M, Konrad L, Rohm KH
  Title
The distribution of aminoacylase I among mammalian species and localization of the enzyme in porcine kidney.
  Journal
Biochimie 82:129-37 (2000)
DOI:10.1016/S0300-9084(00)00191-7
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.14
IUBMB Enzyme Nomenclature: 3.5.1.14
ExPASy - ENZYME nomenclature database: 3.5.1.14
BRENDA, the Enzyme Database: 3.5.1.14
CAS: 9012-37-7

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