KEGG   ENZYME: 3.5.1.78
Entry
EC 3.5.1.78                 Enzyme                                 
Name
glutathionylspermidine amidase;
glutathionylspermidine amidohydrolase (spermidine-forming)
Class
Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In linear amides
Sysname
gamma-L-glutamyl-L-cysteinyl-glycine:spermidine amidase
Reaction(IUBMB)
glutathionylspermidine + H2O = glutathione + spermidine [RN:R01918]
Reaction(KEGG)
R01918
Substrate
glutathionylspermidine [CPD:C05730];
H2O [CPD:C00001]
Product
glutathione [CPD:C00051];
spermidine [CPD:C00315]
Comment
Spermidine is numbered so that atom N-1 is in the amino group of the aminopropyl part of the molecule. The enzyme from Escherichia coli is bifunctional and also catalyses the glutathionylspermidine synthase (EC 6.3.1.8) reaction, resulting in a net hydrolysis of ATP.
History
EC 3.5.1.78 created 1999
Pathway
ec00480  Glutathione metabolism
ec01100  Metabolic pathways
Orthology
K01460  glutathionylspermidine amidase/synthetase
Genes
ECOb2988(gss)
ECJJW2956(gss)
ECDECDH10B_3165(gss)
EBWBWG_2706(gsp)
ECOKECMDS42_2466(gss)
ECEZ4342(gsp)
ECSECs_3873(gss)
ECFECH74115_4300(gsp)
ETWECSP_3967(gsp)
ELXCDCO157_3616
EOIECO111_3818(gss)
EOJECO26_4096(gss)
EOHECO103_3672(gss)
ECOOECRM13514_3891(gsp)
ECOHECRM13516_3759(gsp)
ESLO3K_03980
ESOO3O_21670
ESMO3M_04015
ECKEC55989_3404(gss)
ECGE2348C_3275(gsp)
EOKG2583_3714(gsp)
ELRECO55CA74_17695
ELHETEC_3262
ECWEcE24377A_3455(gsp)
EUNUMNK88_3745
ECPECP_3073
ENAECNA114_3071(gsp)
ECOSEC958_3382
ECVAPECO1_3433(gsp)
ECOAAPECO78_18730
ECXEcHS_A3168(gsp)
ECMEcSMS35_3274(gsp)
ECYECSE_3273
ECRECIAI1_3137(gss)
ECQECED1_3638(gss)
EUMECUMN_3472(gss)
ECTECIAI39_3484(gss)
EOCCE10_3521(gss)
EBRECB_02864(gss)
EBLECD_02864(gss)
EBEB21_02813(gsp)
EBDECBD_0750
ECIUTI89_C3410(gsp)
EIHECOK1_3407(gsp)
ECZECS88_3370(gss)
ECCc3725(gsp)
ELOEC042_3278(gsp)
ELNNRG857_14830
ESEECSF_2819
ECLEcolC_0705
EKOEKO11_0725
EKFKO11_07735(gsp)
EABECABU_c33940(gss)
EDHEcDH1_0708
EDJECDH1ME8569_2888(gsp)
ELUUM146_01415
ELWECW_m3262(gsp)
ELLWFL_15935(gsp)
ELCi14_3416(gsp)
ELDi02_3416(gsp)
ELPP12B_c3091
ELFLF82_0937(gsp)
ECOLLY180_15470
ECOIECOPMV1_03293(gsp_1)
ECOJP423_16880
EFEEFER_2930(gss)
EALEAKF1_ch2960(gsp)
EMAC1192_20565
ESZFEM44_06000
STYSTY3310(gsp)
STTt3060(gsp)
SEXSTBHUCCB_32280
SENTTY21A_15460
STMSTM3139(gsp)
SEOSTM14_3802(gsp)
SEVSTMMW_30991
SEYSL1344_3113(gsp)
SEMSTMDT12_C31940
SEJSTMUK_3127(gsp)
SEBSTM474_3289(gsp)
SEFUMN798_3414(gsp)
SETUSTU288_15895
SETCCFSAN001921_01320
SENRSTMDT2_30331(gsp)
SENDDT104_31351(gsp)
SENICY43_16365
SEENSE451236_21815
SPTSPA3007(gsp)
SEKSSPA2805
SPQSPAB_03921
SEISPC_3208(gsp)
SECSCH_3080(gsp)
SEHSeHA_C3386
SHBSU5_03637
SENHCFSAN002069_16450
SEEHSEEH1578_01830
SEESNSL254_A3392
SENNSN31241_42660
SEWSeSA_A3321
SEASeAg_B3312
SENSQ786_15245
SEDSeD_A3486
SELSPUL_3145(gsp)
SEGASPUCDC_3131(gsp)
SETSEN2982(gsp)
SENAAU38_15165
SENOAU37_15170
SENVAU39_15170
SENQAU40_17095
SENLIY59_15770
SENJCFSAN001992_17850
SEECCFSAN002050_22640
SEEBSEEB0189_004180
SEEPI137_15025
SENBBN855_32150(gsp)
SENEIA1_15160
SENCSEET0819_22700
SESSARI_04484
SBGSBG_2738(gsp)
SBZA464_3170
SBVN643_13630
SALZEOS98_03620
SFLSF3035(gsp)
SFXS3236(gsp)
SFVSFV_3041(gsp)
SFESFxv_3331(gsp)
SFNSFy_4331
SFSSFyv_4411
SFTNCTC1_03289(gsp_1)
SSNSSON_3133(gsp)
SBOSBO_2875(gsp)
SBCSbBS512_E3415(gsp)
SDYSDY_3085(gsp)
SDZAsd1617_04123
SHQA0259_18680
ENCECL_04317
ENLA3UG_19120
ECLGEC036_37110
ECLEECNIH2_19370
ECLNECNIH4_03990
ECLIECNIH5_18175
ECLXLI66_18940
ECLYLI62_20885
ECLZLI64_17800
ECLOENC_32350
EHMAB284_06740
EXFBFV63_18605
ECLAECNIH3_18260
ECLCECR091_18195
EAUDI57_00510
EKBBFV64_19475
ENOECENHK_18750
EECEcWSU1_03782(gsp)
ELGBH714_20860
ECANCWI88_03460
ERNBFV67_18410
ECLSLI67_020445
ECHGFY206_20675
ESHC1N69_19180
ENTEnt638_3393
EASEntas_3655
ENXNI40_018510
LNICWR52_16310
EBGFAI37_10390
ENDA4308_03425
ENZG0034_19165(gss)
ENSHWQ15_04340(gss)
ENKLOC22_07090(gss)
EHUD5067_0003750
EMORL6Y89_18320(gss)
ENBELK40_19195
KPNKPN_03416(gss)
KPUKP1_4691(gss)
KPMKPHS_45430
KPPA79E_0706
KPHKPNIH24_06165
KPZKPNIH27_21685
KPVKPNIH29_22560
KPWKPNIH30_22880
KPYKPNIH31_21800
KPGKPNIH32_23735
KPCKPNIH10_22635
KPQKPR0928_22145
KPTVK055_4058
KPOKPN2242_20170
KPJN559_0765
KPID364_17595
KPAKPNJ1_00752
KPSKPNJ2_00792
KPXPMK1_00892(gsp_1)
KPBFH42_13350
KPNEKU54_003700
KPNULI86_03705
KPNKBN49_0688(gsp)
KVAKvar_0675
KPEKPK_0702(gsp)
KPKA593_07150
KVDKR75_04635
KVQSP68_11160
KOXKOX_02835
KOEA225_5016
KOYJ415_06930
KMIVW41_20185
KOKKONIH1_25170
KOCAB185_10955
KQUAVR78_00945
EAEEAE_03450
EARCCG33297
KQVB8P98_03730
KLLBJF97_25060
KLWDA718_04280
KARLGL98_03615(gss)
KGRJJJ10_03725(gss)
RORRORB6_22230
RONTE10_24110
RPLNB1209_03500
RAODSD31_03700
RTGNCTC13098_00937(gsp_2)
REEelectrica_00668(gsp_2)
CROROD_35371(gsp)
CKOCKO_04379
CFDCFNIH1_02205
CBRAA6J81_21055
CWECO701_01575
CYOCD187_03895
CPOTFOB25_15235
CFQC2U38_21575
CSEDJY391_18760(gss)
CAMAF384_25290
CAFAL524_22950
CIFAL515_19575
CFARCI104_04065
CIRC2U53_05500
CIEAN232_08340
CPARCUC49_15485
CTELGBC03_26840
CITZE4Z61_20710
CARSE1B03_22250
EBTEBL_c18200
CNTJT31_15700
CEMLH23_02000
CENLH86_01980
PGELG71_07735
ESCEntcl_0756
KLEAO703_17795
KGOCEW81_03845
KIENCTC12125_04615(gsp_2)
KASKATP_07050
LPNUKQ929_03030(gss)
LAZA8A57_17815
LEFLJPFL01_3492
LEWDAI21_08385
BUFD8682_25560
BFTMNO13_14590(gss)
SYMK6K13_18935(gss)
YREHEC60_14520(gss)
IZHFEM41_08605
PSGCG163CM_43150(gss)
EBFD782_0692
EBUCUC76_19205
GQUAWC35_03140
BGJAWC36_13915
BIZHC231_18740(gss)
TCIA7K98_07995
TPTYNCTC11468_01666(gsp_2)
PMRPMI0183(gsp)
PMIBBB2000_0343(gsp)
PVLAOB99_03715
PVGCRN77_00315
PHAUPH4a_10915
PROTBTA34_02605
PCOLF1325_03865
PCIBF9282_03685
PPEEI6G31_05610(gss)
PALACO695_14560
PRJNCTC6933_01941(gsp_1)
MMKMU9_3160
MWIMNY66_11630(gss)
EICNT01EI_2998
ETRETAE_2690
ETDETAF_2424
ETEETEE_0790(gsp)
ETCETAC_12900
EDWQY76_07070
EDLAAZ33_14195(gss)
EHOA9798_03845
HAVAT03_19415
HPARAL518_07170
OPODSM2777_05980
PFQQQ39_10560
LRINCTC12151_03225(gsp_2)
HPRPARA_09880
HAYC3V42_05715
HPITNCTC13334_00926(gsp_2)
HHZNCTC10839_00106(gsp_1)
HPAAE5Q53_02020
HSEML3077_03535(gss)
MSUMS0671(gsp)
BSUNA4G13_01175
ASUAsuc_0804
AAPNT05HA_2010
AAZADJ80_02690
AATD11S_0204
AAOANH9381_0534
AAND7S_01327
AAHCF65_00544
AACNAANUM_0246
AACTACT75_06460
ASEGNCTC10977_00377(gsp_2)
EMODM558_01575
SDKKHX94_15980(gss)
SYKKDN34_11135(gss)
SAVIJYB87_06100(gss)
COZA3Q34_03840
PHAPSHAa2455(gssA)
PTNPTRA_a2949(gsp)
PIAPI2015_0588
PBWD172_014785
PALNB0W48_04760
PARTPARC_a0816(gsp)
PTUPTUN_a3222(gsp)
PNGPNIG_a3134(gsp)
PDVFFU37_02065
GNIGNIT_0241(gsp)
GPSC427_5446(gsp)
PMESFX988_01210
PINPing_1235
SAGAM5M_11030
ZALAZF00_15680
KAKKalk_19435
MMWMmwyl1_0216
MPRIMP3633_0175
MARDIBG28_01045(gss)
MFOIJSY38_10155(gss)
MPRFJ8N69_08685(gss)
NCUF0U83_13140
GAPGAPWK_0217
FPPFPB0191_02064
ORBIPMB12_02005(gss)
AZQG3580_01035(gss)
PMASNCF86_16320(gss)
RRFF11_19045
 » show all
Reference
1  [PMID:7775463]
  Authors
Bollinger JM Jr, Kwon DS, Huisman GW, Kolter R, Walsh CT
  Title
Glutathionylspermidine metabolism in Escherichia coli. Purification, cloning, overproduction, and characterization of a bifunctional glutathionylspermidine synthetase/amidase.
  Journal
J Biol Chem 270:14031-41 (1995)
DOI:10.1074/jbc.270.23.14031
  Sequence
[eco:b2988]
Other DBs
ExplorEnz - The Enzyme Database: 3.5.1.78
IUBMB Enzyme Nomenclature: 3.5.1.78
ExPASy - ENZYME nomenclature database: 3.5.1.78
BRENDA, the Enzyme Database: 3.5.1.78
CAS: 171040-71-4

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