KEGG ENZYME: 3.5.4.43

ENZYME: 3.5.4.43

Entry

EC 3.5.4.43                 Enzyme

Name

hydroxydechloroatrazine ethylaminohydrolase;
atzB (gene name);
2,4-dihydroxy-6-(isopropylamino)-1,3,5-triazine ethylaminohydrolase

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In cyclic amidines

Sysname

hydroxyatrazine ethylaminohydrolase

Reaction(IUBMB)

hydroxyatrazine + H2O = N-isopropylammelide + ethylamine [RN:R05559]

Reaction(KEGG)

R05559;
(other) R06966

Substrate

hydroxyatrazine [CPD:C06552];
H2O [CPD:C00001]

Product

N-isopropylammelide [CPD:C06553];
ethylamine [CPD:C00797]

Comment

Contains Zn2+. This bacterial enzyme is involved in degradation of the herbicide atrazine. The enzyme has a broad substrate range, and requires a monohydroxylated s-triazine ring with a minimum of one primary or secondary amine substituent and either a chloride or amine leaving group. It catalyses both deamination and dechlorination reactions.

History

EC 3.5.4.43 created 2000 as EC 3.5.99.3, transferred 2016 to EC 3.5.4.43

Pathway

ec00791

Atrazine degradation

ec01100

Metabolic pathways

ec01120

Microbial metabolism in diverse environments

Orthology

K03382

hydroxydechloroatrazine ethylaminohydrolase

Genes

MESM:

EJ066_30455

MERD:

EB233_28550

EAK:	EKH55_4687

RET:	RHE_PC00089

REL:	REMIM1_PB00101(atzB)

REP:	IE4803_PD00305(atzB)

REI:	IE4771_PE00321(atzB)

RLE:	pRL100336(atzB)

RLT:	Rleg2_4464

RLG:

Rleg_6466

RLB:	RLEG3_02410

RLU:	RLEG12_05310

RTR:	RTCIAT899_PC01345

RHL:	LPU83_pLPU83d0291(atzB)

RGA:	RGR602_PC00659(atzB)

RHK:	Kim5_PD00282(atzB)

RHR:	CKA34_23020

RAD:	CO657_34030

RII:	FFM53_035575

RHID:

FFM81_022560

RBQ:	J2J99_24935

RLN:	J0663_28690

RBAN:

J2J98_28180

RRG:	J3P73_33465

RAW:	NE851_06005

RSUL:

N2599_33325

SZO:	K8M09_20720

PDES:

FE840_020510

MOG:	MMB17_11770

MMED:

Mame_02713(atzB)

LIT:	FPZ52_15265

PPAF:

I8N54_15385

PFAA:

MM59RIKEN_34140

VFA:	MM35RIKEN_14260

BLIQ:

INP51_10675

LACY:

A4V08_06920

CSH:	Closa_1628

CBOL:

CGC65_10900

DOR:	Desor_4162

DMI:	Desmer_2264

LTR:	EVS81_12845

ARX:	ARZXY2_4058

AAU:	AAur_pTC10218(atzB)

ABAO:

BEQ56_00965

SSM:	Spirs_1220

OCK:	EXM22_00140

SBU:	SpiBuddy_0452

SGP:	SpiGrapes_0013

SASS:

MUG09_02485

» show all

Reference

1 [PMID:9055410]

Authors

Boundy-Mills KL, de Souza ML, Mandelbaum RT, Wackett LP, Sadowsky MJ

Title

The atzB gene of Pseudomonas sp. strain ADP encodes the second enzyme of a novel atrazine degradation pathway.

Journal

Appl Environ Microbiol 63:916-23 (1997)
DOI:10.1128/AEM.63.3.916-923.1997

Sequence

[ag:AAC45138]

Reference

2 [PMID:17660279]

Authors

Seffernick JL, Aleem A, Osborne JP, Johnson G, Sadowsky MJ, Wackett LP

Title

Hydroxyatrazine N-ethylaminohydrolase (AtzB): an amidohydrolase superfamily enzyme catalyzing deamination and dechlorination.

Journal

J Bacteriol 189:6989-97 (2007)
DOI:10.1128/JB.00630-07

Sequence

[ag:AAC45138]

Other DBs

ExplorEnz - The Enzyme Database:

3.5.4.43

IUBMB Enzyme Nomenclature:

3.5.4.43

ExPASy - ENZYME nomenclature database:

3.5.4.43

BRENDA, the Enzyme Database:

3.5.4.43

DBGET integrated database retrieval system