KEGG ENZYME: 3.5.5.4

ENZYME: 3.5.5.4

Entry

EC 3.5.5.4                  Enzyme

Name

cyanoalanine nitrilase;
beta-cyanoalanine nitrilase

Class

Hydrolases;
Acting on carbon-nitrogen bonds, other than peptide bonds;
In nitriles

Sysname

3-cyano-L-alanine aminohydrolase

Reaction(IUBMB)

3-cyano-L-alanine + 2 H2O = L-aspartate + NH3 (overall reaction) [RN:R00486];
(1a) 3-cyano-L-alanine + H2O = L-asparagine [RN:R01267];
(1b) L-asparagine + H2O = L-aspartate + NH3 [RN:R00485]

Reaction(KEGG)

R00485 R00486 R01267

Substrate

3-cyano-L-alanine [CPD:C02512];
H2O [CPD:C00001];
L-asparagine [CPD:C00152]

Product

L-aspartate [CPD:C00049];
NH3 [CPD:C00014];
L-asparagine [CPD:C00152]

Comment

L-Asparagine is formed as an intermediate. cf. EC 4.2.1.65, 3-cyanoalanine hydratase and EC 3.5.1.1, asparaginase.

History

EC 3.5.5.4 created 1986

Pathway

ec00460

Cyanoamino acid metabolism

ec01100

Metabolic pathways

ec01110

Biosynthesis of secondary metabolites

Orthology

K13035

beta-cyano-L-alanine hydratase/nitrilase

Genes

AJM:

119059097

BCOO:

119072218 119077145

PJA:

122252283

PCHN:

125042571

PMOO:

119590156

ATH:	AT5G22300(NIT4)

ALY:

9310128

CRB:

17881917

CSAT:

104706517 104736238 104770558

EUS:	EUTSA_v10013949mg

BRP:	103851433(NIT-T4)

BNA:	106384710 106427339

BOE:

106327565

RSZ:

108843167

THJ:

104810929

CPAP:

110806828 110810608

CIT:	102624718 102626612

CIC:	CICLE_v10020864mg CICLE_v10028755mg

PVY:

116105637

MINC:

123209435

TCC:	18590263 18598346

GRA:	105766626 105792876

GHI:	107948779 107953166 107954122

GAB:	108458448 108468291

DZI:	111284196 111302070 111304380 111311679

EGR:	104421327 104421328

GMX:	100778110 100810230

GSJ:	114373604 114380588

PVU:	PHAVU_011G096700g

VRA:

106775914

VAR:

108339531

VUN:

114168387

CCAJ:

109799314

APRC:

113858884

MTR:

25492254

TPRA:

123924730

CAM:

101500877

PSAT:

127107888

LJA:	Lj3g3v2981810.1(Lj3g3v2981810.1) Lj3g3v2981810.2(Lj3g3v2981810.2) Lj3g3v2981810.3(Lj3g3v2981810.3) Lj3g3v2981810.4(Lj3g3v2981810.4)

ADU:

107478319

AIP:

107630108

AHF:	112737863 112789992

LANG:

109351736(NIT4) 109353638(NIT4B)

PCIN:

129312640

FVE:

101314432

RCN:

112165679

PPER:

18770202 18774569

PMUM:

103329255 103341728

PAVI:

110746687 110761217

PDUL:

117631340 117633648

MDM:

103424813

MSYL:

126614863

PXB:	103936462 103966370

ZJU:	107409663 107415031

MNT:

21399366

CSAV:

115701548 115702867

CSV:

101209360

CMO:

103501743

BHJ:

120083021

MCHA:

111012712

CMAX:

111471660

CMOS:

111434356

CPEP:

111784116

RCU:	8263319 8263320 8263321 8263322

JCU:

105635907

HBR:	110639704 110640140

MESC:

110613972 110623491 110626532

POP:	18098088 7465247 7466016

PEU:	105112584 105139048 105141407

PALZ:

118030656 118038453 118038950

JRE:	108990509 108998921 108998953

CILL:

122278121 122291239 122292819

QSU:	111990566 111993928 111993929 111993937

QLO:	115953695 115973063 115978888 115978896

TWL:	119990443 119998437 120002126

VVI:	100240765 100241354 100241359 100243086 100248203 100250098 100253338 100263621 100265384 100853652

VRI:	117906526 117909783 117909785 117916262 117927253 117928568 117928647

SLY:

101266793

SPEN:

107004945

SOT:

102595618

SSTN:

125844989

CANN:

107847983

NTA:	107804379(NIT4A) 107812524 107832013(NIT4B)

NSY:

104235794

NTO:	104091838 104099483

NAU:

109229286

INI:

109160194

ITR:

116004786

SIND:

105170044

OEU:

111383075

EGT:

105970138

SSPL:

121742196 121743274 121743340 121743509 121790517 121790911 121811014 121811478

SMIL:

131000224 131000225 131021509 131021845

APAN:

127253816

HAN:	110899901 110930882

ECAD:

122599842

LSV:

111896404

CCAV:

112529711

DCR:

108215255

CSIN:

114287616

RVL:

131326585

BVG:

104899831

SOE:

110798812

CQI:	110683592 110688144

ATRI:

130812714

MOF:

131149826

NNU:	104589516 104597484 104601345 104603451

MING:

122061235 122068215 122068216 122077137 122077139 122085695

TSS:	122642463 122642544 122649163

PSOM:

113309654 113313402 113322008 113323580

OSA:	4330076 4330077

DOSA:

Os02t0635000-01(Os02g0635000) Os02t0635200-01(Os02g0635200)

OBR:	102701789 102702164

OGL:	127762457 127762458

BDI:	100822233 100822856

ATS:	109746316 109746318

TDC:	119317215 119317216 119317218 119318148 119322478 119322675 119323118

TAES:

100682473 123127803 123127804 123128638 123137562 123137563 123137564 123142683 123144908 123144909

TUA:	125513654 125513655 125514961 125530114

LPER:

127306985 127306986

LRD:	124692503 124692523 124692524

SBI:	110434689 8056027 8074792 8074793

ZMA:	542093 542573

SITA:

101766965 101767363

SVS:	117863514 117863518

PVIR:

120656561 120709983 120709990 120710000 120710008 120710018 120710030

PHAI:

112891912 112891922

PDA:

103705226

EGU:

105042779

MUS:

103994490

ZOF:	121967639 121967641 122052886 122052887

DCT:

110107670

PEQ:

110023533

AOF:

109825016

NCOL:

116267212

ATR:	18426973 18444517

SMO:	SELMODRAFT_133005(NIT4-2) SELMODRAFT_171766(NIT4-1)

PPP:

112274445

CLUP:

CLUP02_16824

CHIG:

CH63R_06581

SGRA:

EX895_006428

» show all

Reference

Authors

Yanase, H., Sakai, T. and Tonomuro, K.

Title

Purification, crystallization and some properties of beta-cyano-L-alanine-degrading enzyme in Pseudomonas sp. 13.

Journal

Agric Biol Chem 47:473-482 (1983)

Other DBs

ExplorEnz - The Enzyme Database:

3.5.5.4

IUBMB Enzyme Nomenclature:

3.5.5.4

ExPASy - ENZYME nomenclature database:

3.5.5.4

BRENDA, the Enzyme Database:

3.5.5.4

CAS:	85638-44-4

DBGET integrated database retrieval system