KEGG   ENZYME: 3.6.1.74
Entry
EC 3.6.1.74                 Enzyme                                 
Name
mRNA 5'-phosphatase;
5'-polynucleotidase;
polynucleotide 5'-phosphohydrolase;
RNGTT (gene name);
CET1 (gene name);
mRNA 5'-triphosphate monophosphatase
Class
Hydrolases;
Acting on acid anhydrides;
In phosphorus-containing anhydrides
Sysname
5'-triphospho-mRNA 5'-phosphohydrolase
Reaction(IUBMB)
a 5'-triphospho-[mRNA] + H2O = a 5'-diphospho-[mRNA] + phosphate
Substrate
5'-triphospho-[mRNA] [CPD:C20864];
H2O [CPD:C00001]
Product
5'-diphospho-[mRNA] [CPD:C02100];
phosphate [CPD:C00009]
Comment
The enzyme, found in eukaryotes and some plus strand RNA viruses (e.g. alphavirus), is involved in mRNA capping. Unlike the eukaryotic enzyme, the viral enzyme requires a purine in the first position of the mRNA. The human enzyme is a multi domain protein that also has the activity of EC 2.7.7.50, mRNA guanylyltransferase.
History
EC 3.6.1.74 created 2021
Orthology
K01098  polynucleotide 5'-triphosphatase
K13917  mRNA-capping enzyme
Genes
HSA8732(RNGTT)
PTR462878(RNGTT)
PPS100993736(RNGTT)
GGO101136858(RNGTT)
PON100439097(RNGTT)
NLE100588336(RNGTT)
MCC702334(RNGTT)
MCF102125723(RNGTT)
CSAB103240079(RNGTT)
CATY105588991(RNGTT)
PANU101001398(RNGTT)
TGE112623302(RNGTT)
RRO104657908(RNGTT)
RBB108532637(RNGTT)
TFN117097068(RNGTT)
PTEH111538067(RNGTT)
CJC100394937(RNGTT)
SBQ101049020(RNGTT)
CSYR103266422(RNGTT)
MMUR105863602(RNGTT)
LCAT123633057(RNGTT)
OGA100957352(RNGTT)
MMU24018(Rngtt)
MCAL110292288(Rngtt)
MPAH110338665(Rngtt)
RNO313131(Rngtt)
MCOC116087980(Rngtt)
MUN110547371(Rngtt)
CGE100770876(Rngtt) 100773893
MAUA101838881(Rngtt)
PLEU114681721 114695435(Rngtt)
MORG121458403(Rngtt)
AAMP119825929(Rngtt)
NGI103733076(Rngtt)
HGL101697192(Rngtt)
CPOC100732003(Rngtt)
DORD105998216(Rngtt)
DSP122107745(Rngtt)
NCAR124988525
OCU100340242(RNGTT)
OPI101535862 101536652(RNGTT)
TUP102495064(RNGTT)
CFA481914(RNGTT)
VVP112908388(RNGTT)
VLG121500237(RNGTT)
AML100483690(RNGTT)
UMR103659864(RNGTT)
UAH113264837(RNGTT)
UAR123798568(RNGTT)
ELK111158833
LLV125102458
MPUF101690994(RNGTT)
ORO101370328(RNGTT)
EJU114213060(RNGTT)
ZCA113927436(RNGTT)
MLX118001301(RNGTT)
FCA101096893(RNGTT)
PYU121029764(RNGTT)
PBG122489749(RNGTT)
PTG102959264(RNGTT)
PPAD109263391(RNGTT)
AJU106972542(RNGTT)
HHV120231871(RNGTT)
BTA511408(RNGTT)
BOM102269511(RNGTT)
BBUB102408950(RNGTT)
CHX102177341(RNGTT)
OAS101111376(RNGTT)
ODA120862736(RNGTT)
CCAD122422548(RNGTT)
SSC100520874(RNGTT)
CFR102516343(RNGTT)
CBAI105064127(RNGTT)
CDK105095541(RNGTT)
VPC102537528(RNGTT)
BACU103013048(RNGTT)
OOR101288281(RNGTT)
DLE111163638(RNGTT)
PCAD102995880(RNGTT)
PSIU116763608(RNGTT)
ECB100065700(RNGTT)
EPZ103549521(RNGTT)
EAI106826688(RNGTT)
MYB102249374(RNGTT)
MYD102770454(RNGTT)
MMYO118659810(RNGTT)
MLF102432858(RNGTT)
MNA107533435(RNGTT)
PKL118705103(RNGTT)
DRO112296942(RNGTT)
SHON118982278(RNGTT)
AJM119038679(RNGTT)
PDIC114494299(RNGTT)
PHAS123822009(RNGTT)
MMF118621042(RNGTT)
RFQ117020572(RNGTT)
PALE102887732(RNGTT)
PGIG120594956(RNGTT)
PVP105296983(RNGTT)
RAY107508669(RNGTT)
TOD119252683(RNGTT)
SARA101543017(RNGTT)
LAV100671236(RNGTT)
DNM101442726(RNGTT)
MDO100025582(RNGTT)
GAS123247489(RNGTT)
SHR100921795(RNGTT)
PCW110192035(RNGTT)
OAA100082839(RNGTT)
GGA421819(RNGTT)
PCOC116240660(RNGTT)
MGP100541550(RNGTT)
CJO107311978(RNGTT)
NMEL110395836(RNGTT)
ACYG106035397(RNGTT)
AFUL116487622(RNGTT)
TGU100232080(RNGTT)
LSR110478776(RNGTT)
SCAN103819287(RNGTT)
PMOA120511792(RNGTT)
OTC121340035(RNGTT)
PRUF121360529(RNGTT)
GFR102031604(RNGTT)
FAB101811251(RNGTT)
PHI102104686(RNGTT)
PMAJ107201707(RNGTT)
CCAE111925738 111925739
CCW104694262(RNGTT)
ETL114063766(RNGTT)
ZAB102069057(RNGTT)
FPG101910294(RNGTT)
FCH102049627(RNGTT)
CLV102089917
EGZ104135255(RNGTT)
NNI104021950(RNGTT)
ACUN113478600(RNGTT)
TALA104364707(RNGTT)
PADL103924648(RNGTT)
ACHC115335617(RNGTT)
AAM106489707(RNGTT)
AROW112974970(RNGTT)
NPD112946564(RNGTT)
DNE112979324(RNGTT)
ASN102376947(RNGTT)
AMJ102570226(RNGTT)
CPOO109311853(RNGTT)
GGN109299876(RNGTT)
PSS102453098(RNGTT)
CMY102935462(RNGTT)
CPIC101947951(RNGTT)
TST117875114(RNGTT)
CABI116818018
MRV120400375(RNGTT)
ACS100560223(rngtt)
PVT110076717(RNGTT)
SUND121921496(RNGTT)
PBI103048279(RNGTT)
PMUR107296765(RNGTT)
PGUT117660756(RNGTT)
VKO123022255(RNGTT)
PMUA114593943(RNGTT)
ZVI118082264(RNGTT)
GJA107115389(RNGTT)
STOW125431640(RNGTT)
XLA399383(rngtt.S)
XTR407900(rngtt)
NPR108785315(RNGTT)
RTEM120937106(RNGTT)
BBUF120998180(RNGTT)
BGAR122935055(RNGTT)
DRE405803(rngtt)
SRX107731501 107742988(rngtt)
SANH107660510 107680298(rngtt)
SGH107554925
CAUA113037664 113063493
PPRM120474125(rngtt)
MAMB125277738(rngtt)
IPU108256101(rngtt)
PHYP113529038(rngtt)
SMEO124401189(rngtt)
TFD113662347(rngtt)
EEE113590000(rngtt)
TRU101079531(rngtt)
TNGGSTEN00030419G001
LCO104936194(rngtt)
CGOB115028774(rngtt)
ELY117270900(rngtt)
PLEP121955307(rngtt)
SLUC116066138(rngtt)
ECRA117961477(rngtt)
PFLV114573592(rngtt)
GAT120808521(rngtt)
PPUG119194746(rngtt)
MSAM119909597(rngtt)
CUD121520906(rngtt)
ALAT119012706(rngtt)
MZE101470505(rngtt)
ONL100693252(rngtt)
OAU116312742(rngtt)
OLA101173353(rngtt)
OML112158300(rngtt)
XMA102225982(rngtt)
XCO114158778(rngtt)
XHE116734325(rngtt)
PRET103457371(rngtt)
PFOR103133411(rngtt)
PLAI106962886(rngtt)
PMEI106911250(rngtt)
GAF122825435(rngtt)
CVG107105147(rngtt)
CTUL119771567(rngtt)
GMU124882252(rngtt)
NFU107377729(rngtt)
KMR108233334(rngtt)
ALIM106525679(rngtt)
NWH119418315(rngtt)
CSEM103381224(rngtt)
SSEN122784009(rngtt)
HHIP117758441(rngtt)
HSP118099331(rngtt)
LCF108873496(rngtt)
SDU111239258(rngtt)
SLAL111661520(rngtt)
XGL120789264(rngtt)
HCQ109508037(rngtt)
BPEC110173038(rngtt)
MALB109962341(rngtt)
BSPL114849883(rngtt)
SASA100380472(rngtt)
OTW112217500(rngtt)
OMY110522104(rngtt)
OGO123994915(rngtt)
ONE115139593(rngtt)
SALP111972809(rngtt)
SNH120019650(rngtt)
ELS105017860(rngtt)
SFM108929338(rngtt)
PKI111843049(rngtt)
AANG118230509(rngtt)
LOC102693195(rngtt)
PSPA121317600(rngtt)
ARUT117411619(rngtt)
LCM102363178(RNGTT)
CMK103186202(rngtt)
RTP109911996(rngtt)
BFO118404722
BBEL109465799
SCLV120337899
SPU577418
DMEDmel_CG1810(mRNA-cap)
DER6550097
DSE6618519
DSIDsimw501_GD15855(Dsim_GD15855)
DYADyak_GE16118
DAN6498788
DSR110180624
DPODpse_GA14791
DPE6599294
DMN108155312
DWI6648910
DGR6565059
DMODmoj_GI15976
DAZ108619342
DNV108656840
DHE111596546
DVI6631663
CCAT101454832
BOD106616714
MDE101893695
SCAC106087451
LCQ111674808
LSQ119607078
HIS119658043
AGAAgaP_AGAP005142
ACOZ120952170
AARA120898157
ASTE118511535
AAG5575290
AALB109431612
CQUCpipJ_CPIJ018510
CPII120425624
CNS116347166 116351382
BCOO119070255 119076789
AME414001
ACER107995092
ALAB122718246
BBIF117207087
BVK117237346
BVAN117160926
BPYO122572696
CCAL108622917
OBB114878850
MGEN117229853
NMEA116428246
CGIG122404724
PFUC122526774
VPS122636961
NVI100113955 100118034
TPRE106649390
CGLO123273181
FAS105269236
DAM107040204
AGIF122852653 122855234 122855508 122855563
CCIN107272598
TCA660879
SOY115879688
CSET123312076
AGB108908923
LDC111513901
APLN108740019
PPYR116172284
OTU111424689
MSEX115456279
BANY112053878
PPOT106099499
PXU106127656
PRAP110994202
SLIU111352536
OFU114350316
API100169498 100169584
HHAL106691572
NLU120350672
PHUPhum_PHUM149080
FOC113217495
TPAL117639158
ZNE110830940
CSEC111869117
DMK116919835 116934547 116936452 123468033 123470576
PVM113804004
PJA122251329
PCHN125047836
HAME121879502
PCLA123746587
PTRU123503527
HAZT108675378
EAF111706330
LSM121118386
PPOI119095886 119095888
DSV119450721
RSAN119383714
RMP119170759
VDE111250669
VJA111261602
DPTE113799883
DFR124495188
SDM118185145
CELCELE_C03D6.3(cel-1)
CBRCBG_03700(Cbr-cel-1) CBG_03761
LGILOTGIDRAFT_213204
BGT106051004
GAE121377111
HRF124148416
HRJ124258779
CRG105322687
PMAX117316469
OSN115223445
NVE5511796
ATEN116300710
XEN124449714
HMG100205625
ATHAT3G09100 AT5G01290 AT5G28210
ALY9307003 9320779
CRB17881235 17890698
CSAT104708977 104734174 104745076 104749285 104764607 104768335
EUSEUTSA_v10000074mg EUTSA_v10020216mg
BRP103855428 103870561 117131528 117131533
BNA106427351 106433828 111215797
BOE106295759 106336393
RSZ108846505 108846508 108860069
THJ104816222 104823280 104827039
CPAP110814241
CIT102609768
CICCICLE_v10011182mg
PVY116134565
MINC123192666 123201994
TCC18597604
GRA105761849 105786527
GHI107898847 107899038 107905646 107941706 107958035
GAB108467315 108473646
DZI111294014 111312022
EGR104426395
GMX100776633 100803597
GSJ114395445 114415507
PVUPHAVU_010G008200g
VRA106753467
VAR108322542
VUN114166056
CCAJ109788592 109815064
APRC113848903 113860645
MTRMTR_4g014920 MTR_7g066810
CAM101496087 101515090
LJALj0g3v0256839.2(Lj0g3v0256839.2) Lj3g3v1603870.2(Lj3g3v1603870.2)
ADU107475134
AIP107625682
AHF112727906 112749454
LANG109339089 109340917
FVE101311588
RCN112193240
PPER18775434
PMUM103338253
PAVI110757331
PDUL117631546
MDM103433569 103450536
PXB103941742 103949448 103959590
ZJU107430745
MNT21402718 21402720
CSV101209960
CMO103491007
BHJ120073197
MCHA111019426
CMAX111466182 111478187
CMOS111438145 111453716
CPEP111803494
RCU8262595
JCU105646640
HBR110661792 110667973
MESC110620874 110622631
POP18100001
PEU105121971
PALZ118037561 118037563 118048390
JRE109000805 109004210
QSU111987380 112006691 112036124
QLO115954106 115976991
TWL119995268 120013062
VVI100256199
VRI117920000
SLY101258480
SPEN107031176
SOT102584702
CANN107847477
NTA107818261
NSY104248191
NTO104100989
NAU109229421
INI109158896 109181493
SIND105161563
OEU111373839
HAN110918265 110936015
ECAD122593609 122608141
LSV111884062
CCAV112507049
DCR108196097 108199385
CSIN114267699 114287661 114319874
BVG104908854
SOE110804970
CQI110719040 110738468
NNU104597018
MING122090070
TSS122660064 122662197
PSOM113303508
OSA4350086 4351709
DOSAOs12t0193200-01(Os12g0193200)
OBR102700564 102702516
BDI100825234 100840366
ATS109749722 109782048
TDC119286366 119295370 119353000 119362063
TAES123043384 123051254 123086552 123091397 123096366 123187213
TUA125530610 125535356
SBI8071691
ZMA100193119 103646942
SITA101755911
SVS117866810
PVIR120644093 120685815
PHAI112902508
PDA103705692 103715187
EGU105048839 105049618
MUS103992112
DCT110110603
PEQ110021775
AOF109832843
ATR18436476
SMOSELMODRAFT_100916
PPP112283864
CRECHLRE_08g376700v5 CHLRE_33g758897v5
CSLCOCSUDRAFT_34803
CVRCHLNCDRAFT_24090
APROF751_1379
OLUOSTLU_36207
OTAOT_ostta02g05080
BPGBathy14g02310
MISMICPUN_112642
MPPMICPUCDRAFT_1330
CCPCHC_T00007589001
SCEYMR180C(CTL1) YPL228W(CET1)
SEUBDI49_4263 DI49_5240
SPAOSPAR_M02950 SPAR_P00480
AGOAGOS_AFL134W
ERCEcym_2161
KLAKLLA0_C16049g
KMXKLMA_70222(CET1)
LTHKLTH0H03234g
VPOKpol_1002p27
ZROZYRO0E07700g
CGRCAGL0D03630g CAGL0E06050g
NCSNCAS_0G01280(NCAS0G01280)
NDINDAI_0F01410(NDAI0F01410)
TPFTPHA_0H01840(TPHA0H01840)
TBLTBLA_0A02200(TBLA0A02200)
TDLTDEL_0F01840(TDEL0F01840)
TGBHG536_0D03940
KAFKAFR_0F01220(KAFR0F01220)
KNGKNAG_0D00910(KNAG0D00910)
ZMKHG535_0B01230
PPAPAS_chr3_0609
DHADEHA2E22726g
PICPICST_82650
PGUPGUG_02806
SPAASPAPADRAFT_135454
LELLELG_00094
CALCAALFM_CR02090CA(CET1)
CTPCTRG_00889
COTCORT_0A01300
CDUCD36_27010(CET1)
CTENCANTEDRAFT_135735
YLIYALI0F07865g
CLUCLUG_01683
CLUSA9F13_13g01287
CAURCJI97_000998
SLBAWJ20_3443(CET1)
PKZC5L36_0C08020
BBRXBRETT_000668
OPAHPODL_05096
SLUDSCDLUD_001144
SSLSS1G_04534
PSCOLY89DRAFT_597664
PNOSNOG_00818
PTEPTT_11682
BZECOCCADRAFT_94150
BSCCOCSADRAFT_35815
BORCOCMIDRAFT_91090
AALTCC77DRAFT_60424
PFJMYCFIDRAFT_191917
NPAUCRNP2_608
SPOSPAC644.04(pct1)
CNECNF04680
CNBCNBF0220
CNGCNAG_06549
CGICGB_F0190C
TMSTREMEDRAFT_31680
TASAA1Q1_07765
PPLPOSPLDRAFT_134921 POSPLDRAFT_134922
TVSTRAVEDRAFT_137237
DSQDICSQDRAFT_112787
PCOPHACADRAFT_261863
SHSSTEHIDRAFT_58548
HIRHETIRDRAFT_317859
PSQPUNSTDRAFT_97900
ADLAURDEDRAFT_111235 AURDEDRAFT_112500
FMEFOMMEDRAFT_97783
GTRGLOTRDRAFT_81647
MRRMoror_3974
CCICC1G_11704
SCMSCHCODRAFT_233597
ABPAGABI1DRAFT100277(AGABI1DRAFT_100277)
ABVAGABI2DRAFT151998(AGABI2DRAFT_151998)
CPUTCONPUDRAFT_56588
SLASERLADRAFT_470739
WSEWALSEDRAFT_15794
WICJ056_004211
PFPPFL1_04601
MRTMRET_3518
PGRPGTG_13426
MLRMELLADRAFT_76245
EHIEHI_035610(17.t00048)
EDIEDI_019080
ACANACA1_143300
PIFPITG_08375
EHXEMIHUDRAFT_106724
TVATVAG_187730
 » show all
Reference
1  [PMID:6094533]
  Authors
Itoh N, Mizumoto K, Kaziro Y.
  Title
Messenger RNA guanlyltransferase from Saccharomyces cerevisiae. II. Catalytic properties.
  Journal
J Biol Chem 259:13930-6 (1984)
Reference
2  [PMID:9473487]
  Authors
Tsukamoto T, Shibagaki Y, Murakoshi T, Suzuki M, Nakamura A, Gotoh H, Mizumoto K.
  Title
Cloning and characterization of two human cDNAs encoding the mRNA capping enzyme.
  Journal
Biochem Biophys Res Commun 243:101-8 (1998)
DOI:10.1006/bbrc.1997.8038
  Sequence
[hsa:8732]
Reference
3  [PMID:10748213]
  Authors
Vasiljeva L, Merits A, Auvinen P, Kaariainen L.
  Title
Identification of a novel function of the alphavirus capping apparatus. RNA 5'-triphosphatase activity of Nsp2.
  Journal
J Biol Chem 275:17281-7 (2000)
DOI:10.1074/jbc.M910340199
Other DBs
ExplorEnz - The Enzyme Database: 3.6.1.74
IUBMB Enzyme Nomenclature: 3.6.1.74
ExPASy - ENZYME nomenclature database: 3.6.1.74
BRENDA, the Enzyme Database: 3.6.1.74

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