KEGG   ENZYME: 4.1.2.45
Entry
EC 4.1.2.45                 Enzyme                                 
Name
trans-o-hydroxybenzylidenepyruvate hydratase-aldolase;
2'-hydroxybenzalpyruvate aldolase;
NsaE;
tHBPA hydratase-aldolase
Class
Lyases;
Carbon-carbon lyases;
Aldehyde-lyases
Sysname
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate hydro-lyase
Reaction(IUBMB)
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate + H2O = salicylaldehyde + pyruvate [RN:R05136]
Reaction(KEGG)
R05136
Substrate
(3E)-4-(2-hydroxyphenyl)-2-oxobut-3-enoate [CPD:C06203];
H2O [CPD:C00001]
Product
salicylaldehyde [CPD:C06202];
pyruvate [CPD:C00022]
Comment
This enzyme is involved in naphthalene degradation. The enzyme catalyses a retro-aldol reaction in vitro, and it accepts a broad range of aldehydes and 4-substituted 2-oxobut-3-enoates as substrates [4].
History
EC 4.1.2.45 created 2010, modified 2011
Pathway
ec00626  Naphthalene degradation
ec01100  Metabolic pathways
ec01120  Microbial metabolism in diverse environments
Orthology
K14585  trans-o-hydroxybenzylidenepyruvate hydratase-aldolase
Genes
PPIND061(nahE)
PFKPFAS1_28640
PSCA458_06700
PSTUUIB01_22650
PBMCL52_14635
ALTambt_17910
CYQQ91_1157
CZACYCME_1270
CYYCPC19_06820
FCEJN531_005675
GBIPG2T_00740 PG2T_05760 PG2T_12860
RMNTK49_07980
BMKDM80_1449(phdJ)
BUQAC233_10870 AC233_11110
BFNOI25_3035 OI25_3053(phdJ)
PARBCJU94_38635
PNAPnap_2479
ACIDCBP33_05390
ACIPCBP36_05840
ACINCBP34_05225
ACISCBP35_13105
DELDelCs14_1760
MASWAM586_27480
RBUPG1C_04265 PG1C_08125
MAADAZF01_18315
CIDP73_0351
CONTQ29_19110
PGVSL003B_4082
NARSaro_3836
NPPPP1Y_AT31250
NPNJI59_00960 JI59_04200 JI59_21720 JI59_21740 JI59_21760 JI59_21850 JI59_21910 JI59_24690
NOGGKE62_05260
SBARH5V43_22420
SPHYCHN51_07055
SPHJBSL82_13970
CNAAB433_00715 AB433_10235 AB433_10410 AB433_18070
ERYCP97_14265
MMANMMAN_22710
SBATG4Z16_01255
 » show all
Reference
1  [PMID:8486638]
  Authors
Kuhm AE, Knackmuss HJ, Stolz A
  Title
Purification and properties of 2'-hydroxybenzalpyruvate aldolase from a bacterium that degrades naphthalenesulfonates.
  Journal
J Biol Chem 268:9484-9 (1993)
Reference
2  [PMID:16804169]
  Authors
Keck A, Conradt D, Mahler A, Stolz A, Mattes R, Klein J
  Title
Identification and functional analysis of the genes for naphthalenesulfonate catabolism by Sphingomonas xenophaga BN6.
  Journal
Microbiology 152:1929-40 (2006)
DOI:10.1099/mic.0.28783-0
  Sequence
Reference
3  [PMID:8002605]
  Authors
Eaton RW
  Title
Organization and evolution of naphthalene catabolic pathways: sequence of the DNA encoding 2-hydroxychromene-2-carboxylate isomerase and trans-o-hydroxybenzylidenepyruvate hydratase-aldolase from the NAH7 plasmid.
  Journal
J Bacteriol 176:7757-62 (1994)
DOI:10.1128/JB.176.24.7757-7762.1994
  Sequence
Reference
4  [PMID:10831455]
  Authors
Eaton RW
  Title
trans-o-Hydroxybenzylidenepyruvate hydratase-aldolase as a biocatalyst.
  Journal
Appl Environ Microbiol 66:2668-72 (2000)
DOI:10.1128/AEM.66.6.2668-2672.2000
Other DBs
ExplorEnz - The Enzyme Database: 4.1.2.45
IUBMB Enzyme Nomenclature: 4.1.2.45
ExPASy - ENZYME nomenclature database: 4.1.2.45
BRENDA, the Enzyme Database: 4.1.2.45

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